| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 5,967,695 | G→T | 12.3% | P18P (CCC→CCA) | amtB ← | ammonium transporter AmtB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,967,695 | 0 | G | T | 12.3% | 105.3 / 3.8 | 39 | P18P (CCC→CCA) | amtB | ammonium transporter AmtB |
| Reads supporting (aligned to +/- strand): ref base G (18/16); new base T (3/2); total (21/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01 | |||||||||||
GACAGCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGTTTCGCCGGTACGGATGC > NC_002947/5967546‑5967832 | gACAGCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCg < 2:186008/150‑1 (MQ=255) aGCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGc > 1:263686/1‑150 (MQ=255) cACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCggg > 1:761309/1‑147 (MQ=255) cACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCggg < 2:761309/147‑1 (MQ=255) cTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAg < 1:14620/150‑1 (MQ=255) cTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAg < 2:263686/150‑1 (MQ=255) ttGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAgg > 1:208236/1‑150 (MQ=255) gATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGGCGGCA‑CTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGAc > 1:225722/1‑150 (MQ=255) gccgccATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTcc < 2:531003/150‑1 (MQ=255) cATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGcc > 2:757680/1‑150 (MQ=255) tAGAACAGGGCCAAGCCCGGAATAGTCCAGAACAGCTCCTACACCGTCGCTGTCAGCATTCAGGCGGTGGCGCCGATGTCCCACCTCGA‑CCCTGCTGCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGCCACTAAGGCTCCTATCCCt > 1:196048/1‑150 (MQ=255) cAGGGCCAAGGCCTGAATCGTCACGAACCGCCACAGCGACTCTGCTGTCAGTATTCGGTCCCTGCCGCCGGAATTAAGCGCTGT‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTAGGAGCCCtattgt < 2:141302/150‑6 (MQ=255) ccAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTAc < 2:381184/150‑1 (MQ=255) ccGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCagag > 2:562743/1‑150 (MQ=255) gAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTAGGAGCCCtatt > 2:670704/1‑131 (MQ=255) tCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTa > 1:593111/1‑134 (MQ=255) tCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTa < 2:593111/134‑1 (MQ=255) aTGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGa > 1:308834/1‑100 (MQ=255) aTGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGa < 2:308834/100‑1 (MQ=255) tGAACAGCACCAACGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGtttt > 2:463767/1‑150 (MQ=255) aCCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTggg > 2:765207/1‑150 (MQ=255) aGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCCGGGCGCCGCCTGGCAGTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCg > 2:457775/1‑150 (MQ=255) cgcCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGtt < 2:208236/150‑1 (MQ=255) tGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGttt < 2:403695/150‑1 (MQ=255) gTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTCCGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTg > 2:246717/1‑150 (MQ=255) gTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTggg > 2:802292/1‑135 (MQ=255) gTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTggg < 1:802292/135‑1 (MQ=255) tCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGa < 1:833554/150‑1 (MQ=255) cAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAg < 1:528825/150‑1 (MQ=255) ccGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTggg > 2:356739/1‑114 (MQ=255) ccGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTggg < 1:356739/114‑1 (MQ=255) ttCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTTTTTTTTTTATCGCGTCGGTATCGGTTTCGCCgg > 2:359743/1‑150 (MQ=255) ttCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGTTTCGCCgg > 2:588040/1‑150 (MQ=255) tGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGTTTCGCCGGTACGGa > 2:175706/1‑150 (MQ=255) cAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGTTTCGCCGGTACGGATGc < 2:196048/150‑1 (MQ=255) cAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGTTTCGCCGGTACGGATGc < 1:562743/150‑1 (MQ=255) gggcttgCCCTGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGt > 2:252801/7‑131 (MQ=255) gggcttgCCCTGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGt < 1:251307/125‑1 (MQ=255) gggcttgCCCTGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGt < 1:252801/125‑1 (MQ=255) gggcttgCCCTGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGt > 2:251307/7‑131 (MQ=255) gCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCAtgtt > 2:134168/1‑61 (MQ=255) gCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCAtgtt < 1:134168/61‑1 (MQ=255) | GACAGCACGTTCTTGGATCGCACCATGCCGCCATAGAACAGGGCCAAGCCCGGAATAGTCATGAACAGCACCAGCGCCGTTGCGGTCAGCATCCAGGCGGTGTCGCCGGAATTAAGCGCTGG‑GGCAGCTTCCTCTGCCAGGGCAAGCCCGGGCATTACGAGGGACAATAGGGCTCCTAGCCCTGCGATCTTACGCAGAGTCATGTTGTTTTCTCCTGGGGCGTTGGGTTTGGTGAGGCTTTTTTGCTGCTTAGATCGCGTCGGTATCGGTTTCGCCGGTACGGATGC > NC_002947/5967546‑5967832 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |