Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,995,072 G→A 8.2% intergenic (+144/+33) PP_5251 → / ← PP_5252 transporter/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,995,0720GA 8.2% 125.9 / 3.1 49intergenic (+144/+33)PP_5251/PP_5252transporter/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (22/23);  new base A (2/2);  total (24/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.96e-01

ATTGATGCCGGCCCTTACGGCCCATTCGCGGGTGAACCC‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCACACCACAAGGCC  >  NC_002947/5994924‑5995217
                                                                                                                                                     |                                                                                                                                                 
aTTGATGCCGGCCCTTACGGCCCATTCGCGGGTGAACCCAGGGCCT‑GGCAGAGCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGt                                                                                                                                                  >  2:590523/1‑150 (MQ=37)
aTTGATGCCGGCCCTTACGGCCCATTCGCGGGTGAACCC‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGt                                                                                                                                                  <  1:758500/150‑1 (MQ=255)
    aTGCCGGCCCTTACGGCCCATTCGCGGGTGAACCC‑GCTCCAACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCAGGTTTACCCGCGAAAGGGCCGGCACTGACGACACAAGAAAGGGACCCGTTAtt                                                                                                                                              >  1:813836/1‑150 (MQ=38)
     tGCCGGCCCTTACGGCCCATTCGCGGGTGAACCC‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTg                                                                                                                                             <  2:511387/150‑1 (MQ=255)
     tGCCGGCCCTTACGGCCCATTCGCGGGTGAACCC‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTg                                                                                                                                             <  1:830603/150‑1 (MQ=255)
          gCCCTTACGGCCCATTCGCGGGTGATCCC‑GTTTTTACATAGATCTGCGTTAGACACACACCGGACGTTGGAAACATGCCGTTGTAGGAGCGGGTTTACCCGCGAAATGGCCGTCACTTAAGACATATGAAAGGTGCCAGTTATTGGcccc                                                                                                                                        <  2:813836/150‑1 (MQ=25)
                  ggCCCATTCGCGGGTGAACCC‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTc                                                                                                                                >  2:515702/1‑150 (MQ=255)
                      cATTCGCGGGTGAACCC‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACAt                                                                                                                            >  1:341144/1‑150 (MQ=255)
                        ttCGCGGGTGAACCC‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCAGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACAtca                                                                                                                          >  1:414843/1‑150 (MQ=255)
                         tCGCGGGTGAACCC‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACAtcag                                                                                                                         >  2:582208/1‑150 (MQ=255)
                           gcgGGTGAACCC‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTg                                                                                                                                             >  1:822628/1‑128 (MQ=255)
                           gcgGGTGAACCC‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTg                                                                                                                                             <  2:822628/128‑1 (MQ=255)
                                    ccc‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCGGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGa                                                                                                              >  1:473010/1‑150 (MQ=255)
                                    ccc‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAATAACTGGcccctttct                                                                                                                                   <  1:749347/129‑6 (MQ=37)
                                    ccc‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAATAACTGGcccctttct                                                                                                                                   >  2:749347/1‑124 (MQ=37)
                                     cc‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTa                                                                                                                                                <  1:749067/115‑1 (MQ=255)
                                     cc‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTa                                                                                                                                                >  2:749067/1‑115 (MQ=255)
                                     cc‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGaa                                                                                                             >  2:140300/1‑150 (MQ=255)
                                     cc‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGaa                                                                                                             >  2:620385/1‑150 (MQ=255)
                                       ctcTCCGACACGGGTCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGaaa                                                                                                            <  2:234570/148‑1 (MQ=255)
                                        gCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAg                                                                                                           <  1:709191/150‑1 (MQ=255)
                                        gCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAg                                                                                                           <  2:828358/150‑1 (MQ=255)
                                          tCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCg                                                                                                         <  1:228144/150‑1 (MQ=255)
                                             tACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAg                                                                                                      <  2:89187/150‑1 (MQ=255)
                                                acaGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGccccc                                                                                                                                       <  1:478070/114‑1 (MQ=255)
                                                acaGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCAc                                                                                                   >  1:502446/1‑150 (MQ=255)
                                                acaGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTAGccccc                                                                                                                                       >  2:478070/1‑114 (MQ=255)
                                                    aTCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGTAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACgggc                                                                                               <  1:590523/150‑4 (MQ=255)
                                                       tGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGc                                                                                            >  2:172638/1‑150 (MQ=255)
                                                             aGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGTGTAGAAAGCGAAGCACGAACAGCCCAgcg                                                                                      <  1:582208/150‑1 (MQ=255)
                                                               acacacacCGGACGTTGGAAACAGGCCGTTGTAGGCGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTGCGCTTAGAAAGCGAAGCACGAACAGCCCACCGCa                                                                                    >  1:158223/1‑150 (MQ=255)
                                                                 acacacCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCAcc                                                                                  >  1:21795/1‑150 (MQ=255)
                                                                    cacCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGaa                                                                                                >  1:202300/1‑133 (MQ=255)
                                                                    cacCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGaa                                                                                                <  2:202300/133‑1 (MQ=255)
                                                                                    cAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTTGAAGTCa                                                               <  2:212982/150‑1 (MQ=255)
                                                                                       gCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGccc                                                                        <  2:98008/138‑1 (MQ=255)
                                                                                       gCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGccc                                                                        >  1:98008/1‑138 (MQ=255)
                                                                                                   gCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAATAACTGGcccct                                                                                                                                       >  2:619339/1‑62 (MQ=21)
                                                                                                   gCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAATAACTGGcccct                                                                                                                                       <  1:619339/63‑2 (MQ=21)
                                                                                                      ggTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGaa                                             <  1:172638/150‑1 (MQ=255)
                                                                                                                        ccGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTgg                           >  1:143539/1‑150 (MQ=255)
                                                                                                                                  aGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCAt                 <  1:69995/150‑1 (MQ=255)
                                                                                                                                    acacAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGCACCCCTGGAAGTCATTTCCCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGc               <  2:341144/150‑1 (MQ=255)
                                                                                                                                    acacAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGc               >  2:761043/1‑150 (MQ=255)
                                                                                                                                        aaGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCacac           <  2:473010/150‑1 (MQ=255)
                                                                                                                                          gAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCAcacca         <  1:515702/150‑1 (MQ=255)
                                                                                                                                                 gCCAGTTATTGGCCACCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCACACCACAAGGcc  >  2:480585/1‑150 (MQ=255)
                                                                                                                                                    aGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTgg                           <  2:558735/122‑1 (MQ=255)
                                                                                                                                                    aGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTgg                           >  1:558735/1‑122 (MQ=255)
                                                                                                                                                     |                                                                                                                                                 
ATTGATGCCGGCCCTTACGGCCCATTCGCGGGTGAACCC‑GCTCCTACACAGATCTGCGGTAGACACACACCGGACGTTGGAAACAGGCCGTTGTAGGAGCGGGTTTACCCGCGAAAGGGCCGGCACTGAAGACACAAGAAAGGGGCCAGTTATTGGCCCCCGTTCGTCACATCAGCTCAGCTTAGAAAGCGAAGCACGAACAGCCCAGCGCACCCCAGAAGCCCTGGAAGTCATTTACCGGCACGCTCGAATGCCGCGCCTTTTCGTGGCTGTGCGCATGCACACCACAAGGCC  >  NC_002947/5994924‑5995217

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑