| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,005,029 | G→C | 11.6% | intergenic (+11/‑165) | PP_5259 → / → ydcJ | LysR family transcriptional regulator/metalloprotein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,005,029 | 0 | G | C | 11.6% | 116.9 / 8.5 | 43 | intergenic (+11/‑165) | PP_5259/ydcJ | LysR family transcriptional regulator/metalloprotein |
| Reads supporting (aligned to +/- strand): ref base G (16/22); new base C (2/3); total (18/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.55e-01 | |||||||||||
CGGTAGCCTGGTGGTGCCGTGGAACCATGCAATGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCC > NC_002947/6004883‑6005171 | cGGTAGCCTGGTGGTGCCGTGGAACCATGCAATGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCgg < 2:756723/150‑1 (MQ=255) gtgCCGTGGAACCATGCAATGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTTTAGCCGcgaa < 2:110016/150‑4 (MQ=255) gTGGAACCATGCAATGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACgg > 2:723932/1‑150 (MQ=255) aaCCATGCAATGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCACTCCTACGGGGAc < 1:200722/150‑1 (MQ=255) aaCCATGCAATGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGAATTGGTCGTGTAG‑GGCAAGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGAc > 2:22558/1‑150 (MQ=255) agtGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAgg > 2:526726/3‑150 (MQ=255) tGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGCGGTCCCCGTAggag < 1:686/150‑3 (MQ=255) cgcAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAGGagcg < 1:526726/150‑1 (MQ=39) cgcAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCCCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAGgagc > 1:298910/1‑149 (MQ=38) cgcAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCCCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAGgagc < 2:298910/149‑1 (MQ=38) cGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGc < 2:227310/150‑1 (MQ=255) aGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCgg < 2:159264/150‑1 (MQ=255) aGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCgg < 1:723932/150‑1 (MQ=255) ttACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAgg < 1:308030/150‑1 (MQ=255) ttACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCCCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAGGAGCGGCTTTAGCCGCGaagag < 2:743070/150‑4 (MQ=25) tGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGt > 2:345200/1‑150 (MQ=255) gCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCaaaaaa < 2:101937/150‑1 (MQ=255) cAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGaa < 2:640345/150‑1 (MQ=255) cAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGaa < 1:373288/150‑1 (MQ=255) agGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGa < 1:345200/150‑1 (MQ=255) gTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGaca > 2:457315/1‑150 (MQ=255) aaaGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCaaaaaa < 2:318795/136‑1 (MQ=255) aaaGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCaaaaaa > 1:318795/1‑136 (MQ=255) aaaGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCCCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAGgagc < 2:117404/96‑1 (MQ=21) aaaGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCCCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAGgagc > 1:117404/1‑96 (MQ=21) aaaGGTGCGGGCGCTGGTGGAATGGATTGG‑CGACAAGTTGCAGGTTTCAATGTCCCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACAcactc > 1:149730/1‑150 (MQ=255) tggtggAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCTCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCTCtt > 1:647653/1‑150 (MQ=255) gtggAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAg < 2:535267/76‑1 (MQ=21) gtggAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCGCCTACGCGGTCCCCGTAg > 1:535267/1‑76 (MQ=25) gtggAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCAACGGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGTCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTtgt > 1:794371/1‑150 (MQ=255) tggAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGCGGTCCCCGTAg > 1:3798/1‑75 (MQ=25) tggAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGCGGTCCCCGTAg < 2:3798/75‑1 (MQ=25) aaTGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAgg < 1:557370/94‑1 (MQ=255) aaTGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAgg > 2:557370/1‑94 (MQ=255) aTTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCAATTTTTTTCGCTTGTGAGCAAAGt > 1:509092/1‑150 (MQ=255) tGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCAATTTTTTTCGCTTGTGAGCAAAGTGc < 2:509092/150‑1 (MQ=255) gAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTc > 2:154516/1‑150 (MQ=255) gTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGNCCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCa > 1:688101/1‑141 (MQ=255) gTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCa < 2:688101/141‑1 (MQ=255) tGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTaa < 1:457315/150‑1 (MQ=255) ccTCTTCGGGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGACTTTAGCCGGGAAAGGGCCGTAAAAAAAAAAAAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTcc < 2:149730/150‑1 (MQ=255) ctctTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTtgt < 1:817647/113‑1 (MQ=255) ctctTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTtgt > 2:817647/1‑113 (MQ=255) | CGGTAGCCTGGTGGTGCCGTGGAACCATGCAATGCGCAGTGCCGGAGCGCATTACCTGGCGTATGCCGAGCATGCGGCAGAGGTGCCAAAGGTGCGGGCGCTGGTGGAATGGATTGG‑CGAGCAGTTGCAGGTTTGACGGCCTCTTCGCGGCTAAAGCCGCTCCTACGGGGACCGCGTAGGCGCGGCTTTAGCCGGGAAAGGGCCGTCGAGGTCGATCAAAAAAGGAATGACACACTCACTTTTTTTCGCTTGTGAGCAAAGTGCATTCCCAGCTTTCATGTAAGCGTCC > NC_002947/6004883‑6005171 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |