| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,089,472 | G→T | 13.3% | D199Y (GAC→TAC) | potF‑IV → | putrescine‑binding protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,089,472 | 0 | G | T | 13.3% | 77.2 / 4.1 | 30 | D199Y (GAC→TAC) | potF‑IV | putrescine‑binding protein |
| Reads supporting (aligned to +/- strand): ref base G (10/16); new base T (2/2); total (12/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01 | |||||||||||
TCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCATATCACCTACTTCCACTCCTCGAAGTACATGGCAGACATCGCCAATGGCGACATCTGTGTGGCAGTGGGCTACAGCGGCAGCTTCTCGCAGGCA > NC_002947/6089323‑6089621 | tCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCg > 1:719663/1‑150 (MQ=255)tCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTGGCCGCCGAACAGCg > 1:694956/1‑150 (MQ=255) cgcgCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACcggc < 2:21710/150‑1 (MQ=255) gcCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGa < 1:304970/150‑1 (MQ=255) aGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACaagaag < 1:80300/150‑1 (MQ=255) cTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGag > 1:356828/1‑149 (MQ=255) tCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGa < 2:565268/150‑1 (MQ=255) tCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGa < 2:356828/150‑1 (MQ=255) tCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGa < 1:765133/150‑1 (MQ=255) aGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGa < 2:311859/150‑1 (MQ=255) agaACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTc < 1:750377/150‑1 (MQ=255) cGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCa < 2:843222/150‑1 (MQ=255) cGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCa < 1:618206/150‑1 (MQ=255) cAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCTACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCatat > 1:166219/1‑150 (MQ=255) aaaGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCTACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCatat > 1:166224/2‑150 (MQ=255) aaGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCTACAAACCGGCGGCCTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCATATc < 2:166219/150‑1 (MQ=255) aaGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCTACAAACCGGCGGAATACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCATATc < 2:166224/150‑1 (MQ=255) ccTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCATATCACCTACTTCCACTCCTCGAAGTa > 2:126857/1‑150 (MQ=255) tCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCATATCACCTACTTCCACTCCTCGAAGTACATgg < 1:126857/150‑1 (MQ=255) cGACTCGCCGTCGGAGATTCTACCTCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCATATCACCTACTTCCACTCCTCGAAGTACATGGc < 2:749814/150‑1 (MQ=255) gACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCATATCACCTACTTCCACTCCTCGAAGTACATGGCa > 1:110171/1‑150 (MQ=255) gACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCATATCACCTACTTCCACTCCTCGAAGTACATGGCa < 2:719663/150‑1 (MQ=255) gACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCATATCACCTACTTCCACTCCTCGAAGTACATGGCa > 1:526559/1‑150 (MQ=255) aCCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGc > 2:650162/1‑80 (MQ=255) aCCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGc < 1:650162/80‑1 (MQ=255) gcgcTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCATATCACCTACTTCCACTCCTCGAAGTACATGGCAGACATCGCCAATGGCGACATCtgtgtg > 2:50128/1‑150 (MQ=255) gcTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCATATCACCTACTTCCACTCCTCGAAGTACATGGCAGACATCGCCAATGGCGACATCTGTGTGGc > 2:618601/1‑150 (MQ=255) gcTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGCCCGACGACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCATATCACCTACTTCCACTCCTCGAAGTACATGGCAGACATCGCCAATGGCGACATCTGTGTGGc > 1:623306/1‑150 (MQ=255) cAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCATATCACCTACTTCCACTCCTCGAAGTACATGGCAGACATCGCCAATGGCGACATCTGTGTGGCAGTg < 2:624368/150‑1 (MQ=255) cTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCATATCACCTACTTCCACTCCTCGAAGTACATGGCAGACATCGCCAATGGCGACATCTGTGTGGCAGTGGGCTAc > 1:635078/1‑150 (MQ=255) aCAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAGCTGAAGCTGAAGATACGCCCGCAAATCACCAACTTCAACTTCTCGAAGGACATGCAAGACATCGACAATGGCGTCATCTGCGGGGTATTGTGCTACAGCGGGAGATTCTCgc > 2:358222/1‑150 (MQ=255) cAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCATATCACCTACTTCCACTCCTCGAAGTACATGGCAGACATCGCCAATGGCGACATCTGTGTGGCAGTGGGCTACAGCGGCAGCTTCTCgca < 1:618601/150‑1 (MQ=255) gACAAACCGGCGGACTACAAGAAGGCCGAGGAGCTGATGCTGAAGATTCGTCCGCATATCACCTGCTTCCACTCCTCGAAGTACATGGCAGACATCGCCAATGGCGACATCTGTGTGGCAGTGGGCTACAGCGGCAGCTTCTCgcaggca < 1:358222/150‑1 (MQ=255) | TCGGCGACAACGCGCCGGTGGACAGCTGGGACCTGATCTTCAAGGAAGAGAACATCGCCAAGCTCAAGCAGTGCGGCGTGGCCCTGCTCGACTCGCCGTCGGAGATTCTACCGCTGGCGCTGCAGTACCTGGGCTTGCCGCCGAACAGCGACAAACCGGCGGACTACAAGAAGGCCGAGGAACTGATGCTGAAGATTCGTCCGCATATCACCTACTTCCACTCCTCGAAGTACATGGCAGACATCGCCAATGGCGACATCTGTGTGGCAGTGGGCTACAGCGGCAGCTTCTCGCAGGCA > NC_002947/6089323‑6089621 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |