| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 6,127,218 | C→G | 11.8% | V241V (GTG→GTC) ‡ | PP_5375 ← | LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 6,127,218 | 0 | C | G | 11.8% | 90.5 / 5.5 | 34 | V241V (GTG→GTC) ‡ | PP_5375 | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (15/15); new base G (2/2); total (17/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01 | |||||||||||
CGGAACAGCCGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTT > NC_002947/6127069‑6127354 | cGGAACAGCCGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGcc > 2:16837/1‑150 (MQ=255) cGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTgg > 1:518410/1‑150 (MQ=255) gccttCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCGTGGCCCTGGcctgg < 2:786835/147‑4 (MQ=255) tttCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTTCCCCAGGCCAGGGCCACGCCCTGGCCAATGa > 2:344823/1‑150 (MQ=255) gacgacTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCGGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCg < 1:16837/150‑1 (MQ=255) gacgacTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCGTGGCCCTGGc > 2:152955/1‑121 (MQ=255) cgacTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGAAGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTa < 1:481207/150‑1 (MQ=255) agcagGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCGTGGCCCTGGcc < 2:767719/113‑1 (MQ=255) agcagGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCGTGGCCCTGGcc > 1:767719/1‑113 (MQ=255) agcagGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTg > 1:647178/1‑150 (MQ=255) ggcagAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGatat < 1:167590/150‑1 (MQ=255) gAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTCGCTGTTGATATTGAc > 2:595092/1‑150 (MQ=255) aCTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGc > 1:557744/1‑112 (MQ=255) aCTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGc < 2:557744/112‑1 (MQ=255) ggCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATg < 1:286259/150‑1 (MQ=255) cTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGt < 1:709196/150‑1 (MQ=255) ggAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTggg < 1:482317/145‑1 (MQ=255) ggAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTggg > 2:482317/1‑145 (MQ=255) cacaTCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTc > 1:290587/1‑150 (MQ=255) caTCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTgg < 2:561100/135‑1 (MQ=255) caTCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTgg > 1:561100/1‑135 (MQ=255) gTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTc < 2:87948/136‑1 (MQ=255) gTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTc > 1:87948/1‑136 (MQ=255) gggCTTGACCAGCGCCCCGGTACTCAACGCATCGTCCTCCAGGTTGCCCCAGGCCAGGGCCACCCCTTGGACACTGCTCGCCTACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAg < 2:517754/150‑1 (MQ=255) gggCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGAcgc > 2:345760/1‑119 (MQ=255) gggCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGAcgc < 1:345760/119‑1 (MQ=255) gCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGgc < 1:595092/150‑1 (MQ=255) gTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGc > 2:671910/1‑150 (MQ=255) aaCGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCt > 1:566228/1‑150 (MQ=255) ccaGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGCTGCTTGGTTGTCATGGGCTc < 2:635264/103‑1 (MQ=255) ccaGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTc > 1:635264/1‑103 (MQ=255) gCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTc < 2:290587/150‑1 (MQ=255) cccAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGACCCGTATCACCGACGACTGACGCATTGGTACCAACCGACCCAGTCGGGCTGGGAGTCtt > 2:8213/1‑150 (MQ=255) aggccaggGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGAcgc < 2:319162/69‑1 (MQ=255) aggccaggGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGAcgc > 1:319162/1‑69 (MQ=255) | CGGAACAGCCGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTT > NC_002947/6127069‑6127354 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |