Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,127,220 C→G 11.8% V241L (GTG→CTG) ‡ PP_5375 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,127,2200CG11.8% 91.4 / 5.4 34V241L (GTG→CTG) ‡PP_5375LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base C (15/15);  new base G (2/2);  total (17/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.82e-01

CGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTT  >  NC_002947/6127078‑6127354
                                                                                                                                              |                                                                                                                                      
cGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTgg                                                                                                                                 >  1:518410/1‑150 (MQ=255)
     gccttCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCGTGGCCCTGGcctgg                                                                                                                            <  2:786835/147‑4 (MQ=255)
       tttCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTTCCCCAGGCCAGGGCCACGCCCTGGCCAATGa                                                                                                                          >  2:344823/1‑150 (MQ=255)
                              gacgacTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCGGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCg                                                                                                   <  1:16837/150‑1 (MQ=255)
                              gacgacTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCGTGGCCCTGGc                                                                                                                                >  2:152955/1‑121 (MQ=255)
                                cgacTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGAAGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTa                                                                                                 <  1:481207/150‑1 (MQ=255)
                                       agcagGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCGTGGCCCTGGcc                                                                                                                               <  2:767719/113‑1 (MQ=255)
                                       agcagGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCGTGGCCCTGGcc                                                                                                                               >  1:767719/1‑113 (MQ=255)
                                       agcagGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTg                                                                                          >  1:647178/1‑150 (MQ=255)
                                           ggcagAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGatat                                                                                      <  1:167590/150‑1 (MQ=255)
                                               gAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTCGCTGTTGATATTGAc                                                                                  >  2:595092/1‑150 (MQ=255)
                                                 aCTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGc                                                                                                                      >  1:557744/1‑112 (MQ=255)
                                                 aCTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGc                                                                                                                      <  2:557744/112‑1 (MQ=255)
                                                     ggCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATg                                                                            <  1:286259/150‑1 (MQ=255)
                                                              cTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGt                                                                   <  1:709196/150‑1 (MQ=255)
                                                                ggAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTggg                                                                      <  1:482317/145‑1 (MQ=255)
                                                                ggAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTggg                                                                      >  2:482317/1‑145 (MQ=255)
                                                                       cacaTCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTc                                                          >  1:290587/1‑150 (MQ=255)
                                                                         caTCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTgg                                                                       <  2:561100/135‑1 (MQ=255)
                                                                         caTCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTgg                                                                       >  1:561100/1‑135 (MQ=255)
                                                                             gTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTc                                                                  <  2:87948/136‑1 (MQ=255)
                                                                             gTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTc                                                                  >  1:87948/1‑136 (MQ=255)
                                                                                gggCTTGACCAGCGCCCCGGTACTCAACGCATCGTCCTCCAGGTTGCCCCAGGCCAGGGCCACCCCTTGGACACTGCTCGCCTACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAg                                                 <  2:517754/150‑1 (MQ=255)
                                                                                gggCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGAcgc                                                                                >  2:345760/1‑119 (MQ=255)
                                                                                gggCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGAcgc                                                                                <  1:345760/119‑1 (MQ=255)
                                                                                  gCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGgc                                               <  1:595092/150‑1 (MQ=255)
                                                                                                   gTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGc                              >  2:671910/1‑150 (MQ=255)
                                                                                                         aaCGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCt                        >  1:566228/1‑150 (MQ=255)
                                                                                                                      ccaGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGCTGCTTGGTTGTCATGGGCTc                                                          <  2:635264/103‑1 (MQ=255)
                                                                                                                      ccaGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTc                                                          >  1:635264/1‑103 (MQ=255)
                                                                                                                             gCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTc    <  2:290587/150‑1 (MQ=255)
                                                                                                                               cccAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGACCCGTATCACCGACGACTGACGCATTGGTACCAACCGACCCAGTCGGGCTGGGAGTCtt  >  2:8213/1‑150 (MQ=255)
                                                                                                                                  aggccaggGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGAcgc                                                                                <  2:319162/69‑1 (MQ=255)
                                                                                                                                  aggccaggGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGAcgc                                                                                >  1:319162/1‑69 (MQ=255)
                                                                                                                                              |                                                                                                                                      
CGCACGCTTTCACGAGGACCACGCTCGGTGGACGACTCCAGCAGGCAGAACTTGGCGTCGGTCTGGAGTACCACATCGTTGGGCTTGACCAGCGCACCGGTGCTCAACGCATCGTCCACCAGGTTGCCCCAGGCCAGGGCCACGCCCTGGCCACTGATCGCCGACTGGATCAGCATACCGTAGCTGTTGATATTGACGCGATGCTTGGGGGTCATGGGCTCGTATCCCAGGCGCTTGAACCATTCGGGCCAACCTACCCAGTCGCGCTGGGAGTCTT  >  NC_002947/6127078‑6127354

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑