Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	6,129,226	C→T	12.8%	intergenic (+32/+35)	PP_5376 → / ← PP_5377	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	6,129,226	0	C	T	12.8%	87.0 / 5.8	39	intergenic (+32/+35)	PP_5376/PP_5377	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (16/18);  new base T (3/2);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.43e-01

GGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCA  >  NC_002947/6129085‑6129373                                                                                                                                              |                                                                                                                                                    ggggAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAgc                                                                                                                                             <  2:828711/150‑1 (MQ=255)    gAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGcc                                                                                                                                          >  2:793203/1‑150 (MQ=255)         tgtgACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTg                                                                                                                                     >  1:500023/1‑150 (MQ=255)           tgACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCg                                                                                                                                   >  2:290693/1‑150 (MQ=255)             aCGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGtt                                                                                                                                 <  1:290693/150‑1 (MQ=255)                  aTGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGaa                                                                                                                            <  1:74177/150‑1 (MQ=255)                         cgcAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTg                                                                                                                     >  2:710636/1‑150 (MQ=255)                            aaaTGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTaaaa                                                                                                                                               >  1:530131/1‑121 (MQ=255)                            aaaTGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTaaaa                                                                                                                                               <  2:530131/121‑1 (MQ=255)                             aaTGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGttt                                                                                                                                      <  1:465446/129‑1 (MQ=255)                             aaTGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGttt                                                                                                                                      >  2:465446/1‑129 (MQ=255)                                     aTGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCt                                                                                                         >  1:303552/1‑150 (MQ=255)                                          gACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATa                                                                                                    >  2:624281/1‑150 (MQ=255)                                            cTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATAct                                                                                                  <  1:793203/150‑1 (MQ=255)                                               gACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTg                                                                                               <  1:710636/150‑1 (MQ=255)                                                        gCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCGttt                                                                                                                                <  1:322688/108‑2 (MQ=37)                                                        gCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCGttt                                                                                                                                >  2:322688/1‑107 (MQ=37)                                                            aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCAATTTAta                                                                                  >  1:342088/1‑149 (MQ=255)                                                            aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCCTTGCTGCCGTTCGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATc                                                                                  <  2:423244/150‑1 (MQ=255)                                                                     gAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCa                                                                                         >  2:143334/1‑134 (MQ=255)                                                                     gAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCa                                                                                         <  1:143334/134‑1 (MQ=255)                                                                     gAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGct                                                                         >  2:403935/1‑150 (MQ=255)                                                                        tACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCtt                                                                      <  1:158046/150‑1 (MQ=255)                                                                            gcgcAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCttgttg                                                                  >  2:353174/1‑150 (MQ=255)                                                                             cgctccTGTCATCAATTGGCGCGTGCCAAATTGATTTCTGAATGCTGTCCAAACGAAGACGCCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTga                                                                 <  1:740181/145‑1 (MQ=255)                                                                                aGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGcc                                                                                                          >  1:829982/1‑106 (MQ=255)                                                                                aGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGcc                                                                                                          <  2:829982/106‑1 (MQ=255)                                                                                     gCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATg                                                         <  1:654249/150‑1 (MQ=255)                                                                                       aTCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCttgtt                                                                   <  1:720497/138‑1 (MQ=255)                                                                                       aTCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCttgtt                                                                   >  2:720497/1‑138 (MQ=255)                                                                                           aTTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTgg                                                   <  2:500023/150‑1 (MQ=255)                                                                                                      cAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATgaga                                        <  1:403935/150‑1 (MQ=255)                                                                                                                     aaTGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTAGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTc                         >  1:811757/1‑150 (MQ=255)                                                                                                                       tGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCa                       >  1:731939/1‑150 (MQ=255)                                                                                                                                aaCGCAAACGGCGCTTTTAAGGCGCCGTTTTCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGgag              >  1:190955/1‑150 (MQ=255)                                                                                                                                    caaaCGGCGCTTTTAAGGCGCCGTTTTCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGa                                                           <  1:393768/100‑1 (MQ=38)                                                                                                                                    caaaCGGCGCTTTTAAGGCGCCGTTTTCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACGTCATCCCGTCCGCTCTTGTTGAGACCGa                                                           >  2:393768/2‑101 (MQ=37)                                                                                                                                         ggCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCg     <  2:811757/150‑1 (MQ=255)                                                                                                                                            gcCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCa  <  1:624281/150‑1 (MQ=255)                                                                                                                                              |                                                                                                                                                    GGGGAAGCTGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCA  >  NC_002947/6129085‑6129373

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑