Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,129,242 G→T 14.5% intergenic (+48/+19) PP_5376 → / ← PP_5377 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,129,2420GT14.5% 88.6 / 5.3 35intergenic (+48/+19)PP_5376/PP_5377hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (13/16);  new base T (3/2);  total (16/19)
Fisher's exact test for biased strand distribution p-value = 6.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.13e-01

TGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATACCTG  >  NC_002947/6129093‑6129379
                                                                                                                                                     |                                                                                                                                         
tgtgACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTg                                                                                                                                           >  1:500023/1‑150 (MQ=255)
  tgACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCg                                                                                                                                         >  2:290693/1‑150 (MQ=255)
    aCGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGtt                                                                                                                                       <  1:290693/150‑1 (MQ=255)
         aTGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGaa                                                                                                                                  <  1:74177/150‑1 (MQ=255)
                cgcAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTg                                                                                                                           >  2:710636/1‑150 (MQ=255)
                            aTGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCt                                                                                                               >  1:303552/1‑150 (MQ=255)
                                 gACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATa                                                                                                          >  2:624281/1‑150 (MQ=255)
                                   cTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATAct                                                                                                        <  1:793203/150‑1 (MQ=255)
                                      gACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTg                                                                                                     <  1:710636/150‑1 (MQ=255)
                                               gCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCGttt                                                                                                                                      <  1:322688/108‑2 (MQ=37)
                                               gCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGCAAACGGCGCTTTTAAGGCGCCGTTTTCGttt                                                                                                                                      >  2:322688/1‑107 (MQ=37)
                                                   aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCAATTTAta                                                                                        >  1:342088/1‑149 (MQ=255)
                                                   aaCATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCCTTGCTGCCGTTCGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATc                                                                                        <  2:423244/150‑1 (MQ=255)
                                                            gAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCa                                                                                               >  2:143334/1‑134 (MQ=255)
                                                            gAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCa                                                                                               <  1:143334/134‑1 (MQ=255)
                                                            gAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGct                                                                               >  2:403935/1‑150 (MQ=255)
                                                               tACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCtt                                                                            <  1:158046/150‑1 (MQ=255)
                                                                   gcgcAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCttgttg                                                                        >  2:353174/1‑150 (MQ=255)
                                                                    cgctccTGTCATCAATTGGCGCGTGCCAAATTGATTTCTGAATGCTGTCCAAACGAAGACGCCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTga                                                                       <  1:740181/145‑1 (MQ=255)
                                                                       aGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGcc                                                                                                                <  2:829982/106‑1 (MQ=255)
                                                                       aGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGcc                                                                                                                >  1:829982/1‑106 (MQ=255)
                                                                            gCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATg                                                               <  1:654249/150‑1 (MQ=255)
                                                                              aTCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCttgtt                                                                         <  1:720497/138‑1 (MQ=255)
                                                                              aTCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCttgtt                                                                         >  2:720497/1‑138 (MQ=255)
                                                                                  aTTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTgg                                                         <  2:500023/150‑1 (MQ=255)
                                                                                             cAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATgaga                                              <  1:403935/150‑1 (MQ=255)
                                                                                                            aaTGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTAGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTc                               >  1:811757/1‑150 (MQ=255)
                                                                                                              tGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCa                             >  1:731939/1‑150 (MQ=255)
                                                                                                                       aaCGCAAACGGCGCTTTTAAGGCGCCGTTTTCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGgag                    >  1:190955/1‑150 (MQ=255)
                                                                                                                           caaaCGGCGCTTTTAAGGCGCCGTTTTCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGa                                                                 <  1:393768/100‑1 (MQ=38)
                                                                                                                           caaaCGGCGCTTTTAAGGCGCCGTTTTCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACGTCATCCCGTCCGCTCTTGTTGAGACCGa                                                                 >  2:393768/2‑101 (MQ=37)
                                                                                                                                ggCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCg           <  2:811757/150‑1 (MQ=255)
                                                                                                                                   gcCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCa        <  1:624281/150‑1 (MQ=255)
                                                                                                                                         aaaGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATACCTg  <  1:353174/150‑1 (MQ=255)
                                                                                                                                                 gTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGAcc                                                            <  2:285039/84‑1 (MQ=255)
                                                                                                                                                 gTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGAcc                                                            >  1:285039/1‑84 (MQ=255)
                                                                                                                                                     |                                                                                                                                         
TGTGACGTGATGCCTGCGCAAATGACCTATGAAGACTCGACAGGCAAGCTCAACATTCTCGAATACCGCGCAGCTGGCATCAATTGCCGCGGCCAAAATTGAGCTCTGAATGCTGTCCAAACGAAAACGGCGCCTTAAAAGCGCCGTTTGCGTTGGGAAAACGGTGGGTCAGTGGCCTGGATACTCTGCAACCACTTCATCCCGTCCGCTCTTGTTGAGACCGATGACCTGGTATGCATGAGAGACACCATCCACTTCCATACCTGGAGAGCCTGCCGGCATACCTG  >  NC_002947/6129093‑6129379

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑