Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,138,500 T→A 9.5% F300Y (TTC→TAC) ‡ czcC → CzcC family metal RND transporter outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,138,5000TA 9.5% 118.0 / 2.8 42F300Y (TTC→TAC) ‡czcCCzcC family metal RND transporter outer membrane protein
Reads supporting (aligned to +/- strand):  ref base T (17/21);  new base A (2/2);  total (19/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.56e-01

GGGGCCATGACCCGTGAAGCACAAGCCCAGGTGCAGCAGGCCATCGCCGAGAAAAAGTCGGATTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGAAGCGGCACTGCGTGAGCACACCGCGCAACTCGAAGCTGACCTGGCGGAATACCAACGCCTACAACGGGCAGTGACGC  >  NC_002947/6138352‑6138649
                                                                                                                                                    |                                                                                                                                                     
ggggCCATGACCCGTGAAGCACAAGCCCAGGTGCAGCAGGCCATCGCCGAGAAAAAGTCGGATTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTc                                                                                                                                                      >  1:553553/1‑150 (MQ=255)
  ggCCATGACCCGTGAAGCACACGCCCAGGAGCAGCCGGTCATCGCCGAGAAAAAGTCGGATTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAg                                                                                                                                                    <  2:496244/150‑1 (MQ=255)
          cccGTGAAGCACAAGCCCAGGTGCAGCAGGCCATCGCCGAGAAAAAGTCGGATTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTcgc                                                                                                                                            <  1:278810/150‑1 (MQ=255)
              tGAAGCACAAGCCCAGGTGCAGCAGGCCATCGCCGAGAAAAAGTCGGATTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCa                                                                                                                                        >  1:243945/1‑150 (MQ=255)
              tGAAGCACAAGCCCAGGTGCAGCAGGCCATCGCCGAGAAAAAGTCGGATTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGAACAGCGGcaatt                                                                                                                                            <  2:798111/146‑5 (MQ=255)
              tGAAGCACAAGCCCAGGTGCAGCAGGCCATCGCCGAGAAAAAGTCGGATTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGAACAGCGGcaatt                                                                                                                                            >  1:798111/1‑142 (MQ=255)
                     caAGCCCAGGTGCAGCAGGCCATCGCCGAGAAAAAGTCGGATTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGAACAGCGGcaa                                                                                                                                              <  2:449984/137‑3 (MQ=255)
                     caAGCCCAGGTGCAGCAGGCCATCGCCGAGAAAAAGTCGGATTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGAACAGCGGcaa                                                                                                                                              >  1:449984/1‑135 (MQ=255)
                      aaGCCCAGGTGCAGCAGGCCATCGCCGAGAAAAAGACGGATTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGCATTCAGCAACATGGTAAGTGTCCAGGGCAGTTTTCAATTGCCGCTGCTCATCGGCTCGCGGCAAGACCCga                                                                                                                                >  1:170735/1‑150 (MQ=255)
                              gTGCAGCAGGCCATCGCCGAGAAAACGCCGTTTTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCgct                                                                                                                        >  1:240479/1‑150 (MQ=255)
                              gTGCAGCAGGCCATCGCCGAGAAAAAGTCGGATTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCgct                                                                                                                        <  2:287762/150‑1 (MQ=255)
                              gTGCAGCAGGCCATCGCCGAGAAAAAGTCGGATTGGGGCTGGCAGGTCGACGACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCgct                                                                                                                        <  1:480092/150‑1 (MQ=255)
                                        ccATCGCCGAGAAAAAGTCGGATTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCggg                                                                                                              <  1:804375/150‑1 (MQ=255)
                                              ccGAGAAAAAGTCGGATTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAgg                                                                                                        >  1:822582/1‑150 (MQ=255)
                                                       aGTCGGATTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGc                                                                                               <  2:445325/150‑1 (MQ=255)
                                                             aTTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTggagg                                                                                         >  1:10402/1‑150 (MQ=255)
                                                                       gCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTgga                                                                                           >  1:60341/1‑138 (MQ=255)
                                                                       gCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTgga                                                                                           <  2:60341/138‑1 (MQ=255)
                                                                        cAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGaa                                                                              <  2:240479/150‑1 (MQ=255)
                                                                            tCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGAAGCgg                                                                          >  1:306109/1‑150 (MQ=255)
                                                                                   ccAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGAAGCGGCACTGCg                                                                   >  1:456493/1‑150 (MQ=255)
                                                                                   ccAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATAGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGAAGCGGCACTGCg                                                                   <  2:553553/150‑1 (MQ=255)
                                                                                    cAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGAAGCGGCACTGCGt                                                                  <  2:243945/150‑1 (MQ=255)
                                                                                     aaCGCCGCGGGCCCGAATTCATCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACTGGAAGCGGCACTGCGTg                                                                 <  2:439746/150‑1 (MQ=255)
                                                                                      aCGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGAAGCGGCACTGCGTGa                                                                <  1:697648/150‑1 (MQ=255)
                                                                                         ccgcGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGTTGCGTTAGCTGGAGGTCGGACAGGAGTCGGCACTGCGTGAGca                                                             >  2:393989/1‑150 (MQ=255)
                                                                                                 ccGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCgat                                                                                                                               >  2:827322/1‑76 (MQ=255)
                                                                                                 ccGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCgat                                                                                                                               <  1:827322/76‑1 (MQ=255)
                                                                                                            aaCATGGTCAGTGTCCAGGTGAGTTTTCAATTGCCGCTGTTCAGCGGCGCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGGGAGCTGGAGGACGAACAGGAAGCGGCACTGCGTGAGGCCCCCGCGCAACTCGAAGCt                                          >  1:373253/1‑150 (MQ=255)
                                                                                                                   tCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGATGACGAACAGGAAGCGGCACTGCGTGAGCACACCGCGCAACTCGAAGCTGACCTgg                                   <  1:532542/150‑1 (MQ=255)
                                                                                                                   tCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGAAGCGGCACTGCGTGAGCACACCGCGCAACTCGAAGCTGACCTgg                                   >  2:456217/1‑150 (MQ=255)
                                                                                                                        gcccAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGAAGCGGCACTGCGTGAGCACACCGCGCAACTCGAAGCTGACCTGGCGGaa                              <  1:393989/148‑1 (MQ=255)
                                                                                                                            aGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCt                                                                                              >  1:43575/1‑82 (MQ=255)
                                                                                                                            aGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCt                                                                                              <  2:43575/82‑1 (MQ=255)
                                                                                                                               tCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGAAGCGGCACTGCGTGAGCACACCGCGCAACTCGAAGCTGACCTGGCGGAATACCAAc                       <  1:412440/150‑1 (MQ=255)
                                                                                                                               tCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGAAGCGGCACTGCGTGAGCACACCGCGCAACTCGAAGCTGACCTGGCGGAATACCAAc                       <  2:163245/150‑1 (MQ=255)
                                                                                                                                   ttttCACTTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGAAGCGGCACTGCGTGAGCACACCGCGCAACTCGAAGCTGACCTGGCGGAATACCAACGCCt                   >  2:374725/1‑150 (MQ=255)
                                                                                                                                   ttttCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGAAGCGGCACTGCGTGAGCACACCGCGCAACTCGAAGCTGACCTGGCGGAATACCAACGCCt                   >  1:452467/1‑150 (MQ=255)
                                                                                                                                             ccGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGAAGCGGCACTGCGTGAGCACACCGCGCAACTCGAAGCTGACCTGGCGGAATACCAACGCCTACAACGGGCa         <  2:456493/150‑1 (MQ=255)
                                                                                                                                                cTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGAAGCGGCACTGCGTGAGCACACCGCGCAACTCGAAGCTGACCTGGCGGAATACCAACGc                     >  1:624598/1‑135 (MQ=255)
                                                                                                                                                cTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGAAGCGGCACTGCGTGAGCACACCGCGCAACTCGAAGCTGACCTGGCGGAATACCAACGc                     <  2:624598/135‑1 (MQ=255)
                                                                                                                                                  gTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCGGTTTCGTCAGCTGGAGGACGAACAGGAAGCGGCACTGCGTGAGCACACCGCGCAACTCGAAGCTGACCTGGCGGAATACCAACGCCTACAACGGGCAGTGAc    <  1:53088/150‑1 (MQ=255)
                                                                                                                                                    tCAGCGGCTCGCGTCAAGACCCGATGATCGCTGTTCGTCGAGCCGAGGTGTGTTAGCTGAAGGGCGAACAGTAAGCTTCACTGCGTGAGCACACCGCACAACTCGAAGCGGGCCTGGCGGAATACCAACGCCTACGACGAGCAGTGAcgc  <  2:170735/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
GGGGCCATGACCCGTGAAGCACAAGCCCAGGTGCAGCAGGCCATCGCCGAGAAAAAGTCGGATTGGGGCTGGCAGGTCGACTACCAACGCCGCGGGCCCGAATTCAGCAACATGGTCAGTGTCCAGGTCAGTTTTCAATTGCCGCTGTTCAGCGGCTCGCGGCAAGACCCGATGATCGCTGCTCGCCGGGCCCAGGTGCGTCAGCTGGAGGACGAACAGGAAGCGGCACTGCGTGAGCACACCGCGCAACTCGAAGCTGACCTGGCGGAATACCAACGCCTACAACGGGCAGTGACGC  >  NC_002947/6138352‑6138649

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑