Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 6,164,793 C→A 11.8% G182G (GGG→GGT)  PP_5405 ← transposase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029476,164,7930CA11.8% 94.6 / 3.8 34G182G (GGG→GGT) PP_5405transposase
Reads supporting (aligned to +/- strand):  ref base C (13/17);  new base A (2/2);  total (15/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.05e-01

GTAAGGATGTCGCTGTAGATCTTATTTTGAGTCTTGTGGGGGGATTTATTGGCGAGGAACCCCTTTAGCAACCTCTGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGTAGAACCAGAGCCTTGTCCTGGCTGCGTTCTGATGGGGTCGTTGAGCTTTGGCGTAGGCG  >  NC_002947/6164644‑6164937
                                                                                                                                                     |                                                                                                                                                
gTAAGGATGTCGCTGTAGATCTTATTTTGAGTCTTGTGGGGGGATTTATTGGCGAGGAACCCCTTTAGCAACCTCTGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGc                                                                                                                                                  >  1:76781/1‑150 (MQ=255)
           gCTGTAGATCTTATTTTGAGTCTTGTGGGGGGATTTATTGGCGAGGAACCCCTTTAGCAACCTCTGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTtc                                                                                                                                       <  2:554027/150‑1 (MQ=255)
             tGTAGATCTTATTTTGAGTCTTGTGGGGGGATTTATTGGCGAGGAACCCCTTTAGCAACCTCTGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTtctc                                                                                                                                     >  1:749992/1‑150 (MQ=255)
               tAGATCTTATTTTGAGTCTTGTGGGGGGATTTATTGGCGAGGAACCCCTTTAGCAACCTCTGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGa                                                                                                                                   <  2:352683/150‑1 (MQ=255)
                 gATCTTATTTTGAGTCTTGTGGGGGGATTTATTGGCGAGGAACCCCTTTAGCAACCTCTGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGAtt                                                                                                                                 <  1:413818/150‑1 (MQ=255)
                     ttATTTTGAGTCTTGTGGGGGGATTTATTGGCGAGGAACCCCTTTAGCAACCTCTGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGcc                                                                                                                                           >  2:83437/1‑136 (MQ=255)
                     ttATTTTGAGTCTTGTGGGGGGATTTATTGGCGAGGAACCCCTTTAGCAACCTCTGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGcc                                                                                                                                           <  1:83437/136‑1 (MQ=255)
                     ttATTTTGAGTCTTGTGGGGGGATTTATTGGCGAGGAACCCCTTTAGCAACCTCTGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCAt                                                                                                                             >  1:327092/1‑150 (MQ=255)
                                cTTGTGGGGGGATTTATTGGCGAGGAACCCCTTTAGCAACCTCTGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACg                                                                                                                  <  2:749992/150‑1 (MQ=255)
                                                         aaCCCCTTTAGCAACCTCTGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCAc                                                                                         >  1:398815/1‑150 (MQ=255)
                                                              cTTTAGCAACCTCTGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGt                                                                                    >  2:430888/1‑150 (MQ=255)
                                                                ttAGCAACCTCTGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTcc                                                                                  <  2:327092/150‑1 (MQ=255)
                                                                  aGCACCCTCTGCTGTATTGCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGa                                                                                <  2:239323/150‑1 (MQ=255)
                                                                  aGCAACCTCTGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGa                                                                                >  1:401074/1‑150 (MQ=255)
                                                                     aaCCTCTGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCa                                                                             >  2:591951/1‑150 (MQ=255)
                                                                        ctctGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTa                                                                                      <  2:798512/138‑1 (MQ=255)
                                                                        ctctGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAac                                                                          >  1:95114/1‑150 (MQ=255)
                                                                        ctctGCTGCATTTCTGCATCGCAACGACAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTa                                                                                      >  1:798512/1‑138 (MQ=255)
                                                                                 aTTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCCCTTATTCCGACCAAACTCCATGTAg                                                                 <  2:76781/150‑1 (MQ=255)
                                                                                   ttCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGa                                                               <  2:401074/150‑1 (MQ=255)
                                                                                       gCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGTAg                                                           <  2:798808/150‑1 (MQ=255)
                                                                                              aaCGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGTAGAACCaga                                                    >  2:465788/1‑150 (MQ=255)
                                                                                                   tAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGTAGAACCAGAGCCtt                                               <  1:591951/150‑1 (MQ=255)
                                                                                                      ccATGCCTTTTTCCCTTGCGAGAGGGCCGACCAGGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGt                                                             >  2:799993/1‑133 (MQ=255)
                                                                                                      ccATGCCTTTTTCCCTTGCGAGAGGGCCGACCAGGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGt                                                             <  1:799993/133‑1 (MQ=255)
                                                                                                         tGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGTAGAACCAGAGCCTTGTCCTg                                         >  1:66637/1‑150 (MQ=255)
                                                                                                                   cctagacagagGGCCGATAAAGCTTCGTCATATGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGTAGAACCAGAGCCTTGTCCTGGCTGCGTTCt                               <  2:398815/143‑1 (MQ=255)
                                                                                                                        cGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGTAGAACCAGAGCCTTGTCCTGGCTGCGTTCTGATgg                          <  1:465788/150‑1 (MQ=255)
                                                                                                                                            cGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGTAGAACCAGAGCCTTGTCCTGGCTGCGTTCTGATGGGGTCGTTGAGCTTTGGCGTa      <  2:95114/150‑1 (MQ=255)
                                                                                                                                              tCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGTAGAACCAGAGCCTTGTCCTGGCTGCGTTCTGATGGGGTCGTTGAGCTTTGGCGTAgg    <  2:66637/150‑1 (MQ=255)
                                                                                                                                                aTACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGTAGAACCAGAGCCTTGTCCTGGCTGCGTTCTGATGGGGTCGTTGAGCTTTGGCGTAGGCg  >  2:549877/1‑150 (MQ=255)
                                                                                                                                                aTACGACCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGTAGAACCAGAGCCTTGTCCTGGCTGCGTTCTGATggg                         <  2:340439/127‑1 (MQ=255)
                                                                                                                                                aTACGACCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGTAGAACCAGAGCCTTGTCCTGGCTGCGTTCTGATggg                         <  2:340476/127‑1 (MQ=255)
                                                                                                                                                aTACGACCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGTAGAACCAGAGCCTTGTCCTGGCTGCGTTCTGATggg                         >  1:340476/1‑127 (MQ=255)
                                                                                                                                                aTACGACCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGTAGAACCAGAGCCTTGTCCTGGCTGCGTTCTGATggg                         >  1:340439/1‑127 (MQ=255)
                                                                                                                                                     |                                                                                                                                                
GTAAGGATGTCGCTGTAGATCTTATTTTGAGTCTTGTGGGGGGATTTATTGGCGAGGAACCCCTTTAGCAACCTCTGCTGCATTTCTGCATCGCAACGATAACCATGCCTTTTTCCCTTGCGAGAGGGCCGACCAAGCTTCGTCATACGCCCTGGCCCTTCTCGATTCCATGCCCCAATACGGTGAAGAGGTGGCAGCAAGGACCACTTAGTCCGACCAAACACCATGTAGGAGTAGAACCAGAGCCTTGTCCTGGCTGCGTTCTGATGGGGTCGTTGAGCTTTGGCGTAGGCG  >  NC_002947/6164644‑6164937

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑