Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 571,620 G→A 13.7% intergenic (+43/+48) ssb → / ← PP_0486 single‑stranded DNA‑binding protein/GntR family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947571,6200GA13.7% 120.5 / 11.6 51intergenic (+43/+48)ssb/PP_0486single‑stranded DNA‑binding protein/GntR family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (23/21);  new base A (5/2);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.48e-01

CCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCGATGCGCAC  >  NC_002947/571484‑571769
                                                                                                                                        |                                                                                                                                                      
cccGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGtt                                                                                                                                                      <  1:344092/139‑1 (MQ=255)
cccGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGtt                                                                                                                                                      >  2:344092/1‑139 (MQ=255)
          gCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAACTGCCCTggg                                                                                                                                              >  2:55648/1‑137 (MQ=255)
          gCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAACTGCCCTggg                                                                                                                                              <  1:55648/137‑1 (MQ=255)
           ccccTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGgca                                                                                                                                >  2:207246/1‑150 (MQ=255)
           ccccTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGgca                                                                                                                                <  2:235722/150‑1 (MQ=255)
           ccccTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGACATCAAGAATTCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGgca                                                                                                                                <  1:149283/150‑1 (MQ=255)
            cccTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGgcag                                                                                                                               >  1:587000/1‑150 (MQ=255)
             ccTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTg                                                                                                                                                <  2:254120/132‑1 (MQ=255)
             ccTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTg                                                                                                                                                >  1:254120/1‑132 (MQ=255)
             ccTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGgcagc                                                                                                                              >  1:523527/1‑150 (MQ=255)
              cTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGAAGCAGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGTTCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGgcagca                                                                                                                             >  2:313349/1‑150 (MQ=255)
                   cgcccagcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACcgcg                                                                                                                        <  1:207246/150‑1 (MQ=255)
                       cagcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATc                                                                                                                    <  2:291260/150‑1 (MQ=255)
                         gcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAg                                                                                                                  >  2:182703/1‑150 (MQ=255)
                         gcgccGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAg                                                                                                                  <  1:631855/150‑1 (MQ=255)
                                aaCAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCAAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGc                                                                                                           >  1:480302/1‑150 (MQ=255)
                                     ccTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCa                                                                                                      <  2:480302/150‑1 (MQ=255)
                                      cTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTgg                                                                                                                                               >  1:644332/1‑108 (MQ=255)
                                      cTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTgg                                                                                                                                               <  2:644332/108‑1 (MQ=255)
                                        gcgcCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTa                                                                                                   <  1:23392/150‑1 (MQ=255)
                                                  cggcggCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCATAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAg                                                                                                                  <  1:36321/125‑1 (MQ=255)
                                                  cggcggCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAg                                                                                                                  >  2:36321/1‑125 (MQ=255)
                                                             ttCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATc                                                                                                                    <  1:688845/112‑1 (MQ=255)
                                                             ttCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATc                                                                                                                    >  2:688845/1‑112 (MQ=255)
                                                             ttCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCg                                                                                 >  2:12303/1‑146 (MQ=255)
                                                             ttCCACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCg                                                                                 <  1:12303/146‑1 (MQ=255)
                                                                     cTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTa                                                                                                   <  2:344261/121‑1 (MQ=255)
                                                                     cTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTa                                                                                                   >  1:344261/1‑121 (MQ=255)
                                                                      ttCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGa‑a                                                                                               <  2:837408/123‑1 (MQ=255)
                                                                      ttCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGa‑a                                                                                               >  1:837408/1‑123 (MQ=255)
                                                                      ttCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAAAAACCCAGGGCAACTGCCCTGGGtttctg                                                                                                                                        >  2:109930/1‑80 (MQ=37)
                                                                      ttCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAAAAACCCAGGGCAACTGCCCTGGGtttctg                                                                                                                                        <  1:109930/83‑4 (MQ=37)
                                                                                   ttCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTg                                                       >  2:248050/1‑150 (MQ=255)
                                                                                       gTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCa                                                   <  2:523527/150‑1 (MQ=255)
                                                                                            gATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCa                                              >  2:775372/1‑150 (MQ=255)
                                                                                             aTCAGAGCGGTAGGGCGTTCAGCAAGACCAGACACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGGGAATCCGGGGAGCCGAGCAGCATATCCAGCCGGGGC‑GTGTCGAATACAGCAGGCTTGCCGGCTTGGTCAGGGCaa                                             >  1:740195/1‑150 (MQ=255)
                                                                                             aTCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCAACGGATAGCAGGCATCGCTTTCAGTAGA‑AAACTCGGGGTTCCGGTTAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCaa                                             >  1:405542/1‑150 (MQ=255)
                                                                                              tCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCCTTCAGTAGA‑ACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCaaa                                            <  2:138120/150‑1 (MQ=255)
                                                                                                         gggCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCtt                                 <  1:313349/150‑1 (MQ=255)
                                                                                                         gggCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCtt                                 <  2:548283/150‑1 (MQ=255)
                                                                                                                           gAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGACAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCa               >  1:520793/1‑150 (MQ=255)
                                                                                                                              aCCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTgg                               <  1:66222/131‑1 (MQ=255)
                                                                                                                              aCCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTgg                               >  2:66222/1‑131 (MQ=255)
                                                                                                                               cccAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATc                                                                                                             <  2:125274/53‑1 (MQ=255)
                                                                                                                               cccAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATc                                                                                                             >  1:125274/1‑53 (MQ=255)
                                                                                                                               cccAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGc           >  1:47740/1‑150 (MQ=255)
                                                                                                                               cccAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGc           >  2:433198/1‑150 (MQ=255)
                                                                                                                               cccAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGc           >  2:363246/1‑150 (MQ=255)
                                                                                                                                ccAGGGCAACTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCg          >  1:792771/1‑150 (MQ=255)
                                                                                                                                       aGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCGATGCGca   >  1:811935/1‑150 (MQ=255)
                                                                                                                                        gTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCGTCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCGATGCGcac  <  2:740195/150‑1 (MQ=255)
                                                                                                                                        |                                                                                                                                                      
CCCGCAACAGGCCCCTCAGCGCCCAGCGCCGCAACAGCCTGCGCCGCAGCCGGCGGCTGACTTCGACAGCTTCGATGACGATATTCCGTTCTGATCAGAGCGGTAGGGCGTTCAGCAAGACCAGAAACCCAGGGCAGTTGCCCTGGGTTTTTTTATGGGCAGCACCGCGTATCAGGCATCGCTTTCAGTAGA‑ACAACCGGGGCTCCGGCGAATCCAGCAGGCTTGCCAGCTTGGTCAGGGCAAAGCCCAGTTCTTGGCGGCTGGGTGCCATCAGGGCGATGCGCAC  >  NC_002947/571484‑571769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑