| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 572,528 | C→T | 14.3% | V189M (GTG→ATG) | PP_0486 ← | GntR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 572,528 | 0 | C | T | 14.3% | 79.3 / 6.5 | 35 | V189M (GTG→ATG) | PP_0486 | GntR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (13/17); new base T (3/2); total (16/19) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.42e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01 | |||||||||||
GCCTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGAGTACCTGCCCCAAGTCCGA > NC_002947/572386‑572675 | gCCTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGGTGGCCATCagcagc < 1:508110/150‑1 (MQ=255) tCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGGTGGCCATCagcagc < 2:129187/147‑1 (MQ=255) tCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGGTGGCCATCagcagc > 1:129187/1‑147 (MQ=255) ggATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGtt < 1:227603/150‑1 (MQ=255) gTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAg < 1:812287/150‑1 (MQ=255) ccATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGta > 1:446600/1‑150 (MQ=255) ccATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGACCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGta < 1:119127/150‑1 (MQ=255) cgccgcGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACctgct < 1:808489/150‑1 (MQ=255) aGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGa < 2:77182/150‑1 (MQ=255) gCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAgg < 1:186351/150‑1 (MQ=255) ccAGGCTGTGGGGCATGGATATGCTGCCTGGTTAGCAGAGCGACTCGCACAGCACCACATCGTCACTATTCGACAGGCTGCTGCTGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGca > 2:54111/1‑150 (MQ=255) ccAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGca < 1:110624/150‑1 (MQ=255) cagcctgGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGcagcc > 2:814695/6‑150 (MQ=255) tgGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAgcgc < 2:206021/150‑1 (MQ=255) ccAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTa > 2:232276/1‑150 (MQ=255) tgCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCAc > 1:411998/1‑150 (MQ=255) gggTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCGGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCt > 1:152431/1‑150 (MQ=255) gCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGGGGTCAGtactac > 1:270912/1‑93 (MQ=255) gCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGtactac < 2:270912/93‑1 (MQ=255) gagCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGcagc < 2:350763/115‑1 (MQ=255) gagCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGcagc > 1:350763/1‑115 (MQ=255) gCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCa < 2:226309/95‑1 (MQ=255) gCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCa > 1:226309/1‑95 (MQ=255) aTCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTgc < 2:295381/150‑2 (MQ=255) gTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTcac < 2:446600/150‑1 (MQ=255) tCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTcaca > 2:774260/1‑150 (MQ=255) aCTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAg > 1:198536/1‑83 (MQ=255) aCTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAg < 2:198536/83‑1 (MQ=255) ttCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCg > 2:655808/1‑150 (MQ=255) tCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCGGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGa < 2:152431/150‑1 (MQ=255) cAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCttgtttgt > 2:176479/1‑113 (MQ=255) cAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCttgtttgt < 1:176479/113‑1 (MQ=255) gctgctGGTGGCCATCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGAGTACCTg > 1:723840/1‑150 (MQ=255) tgctgGTGGCCATCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGAGTACCTGcc > 2:309019/1‑150 (MQ=255) gATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGAGTACCTGCCCCaa < 1:232276/150‑1 (MQ=255) caccaGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGAGTACCTGCCCCAAGTCCGa < 1:774260/150‑1 (MQ=255) | GCCTCCGGGATGAGCCCGTGTTCGTCCATGGCGACGCCGCGCAGGCGCCGGCCCAGGCTGTGGGCCAGGGATATGATGCCCGGGTAGCAGAGCGACTCGCACAGCACCACATCGTCACTCTTCGACAGGCTGCTGATGGCCACCAGCAGCCCATGTTGGGCGCCAGCGGTCAGTACTACCTGCTGCCAGCGAGCATCAGGCAGCCAGCGCTGTATCCAGGTCGCACCGGCTTGTTTGTGGGACGTGTGACCACCCTCTGTCACATAGTCGAGTACCTGCCCCAAGTCCGA > NC_002947/572386‑572675 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |