Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	626,559	T→G	9.0%	K213Q (AAG→CAG)	dedA ←	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	626,559	0	T	G	9.0%	116.9 / 3.4	44	K213Q (AAG→CAG)	dedA	inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (23/17);  new base G (2/2);  total (25/19)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

GTCGCTGATGCCGTCCAGCATTGGCAGCCGCTCGCGTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGACCAGGCCGC  >  NC_002947/626414‑626702                                                                                                                                                  |                                                                                                                                                gTCGCTGATGCCGTCCAGCATTGGCAGCCGCTCGCGTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGcc                                                                                                                                             >  2:794749/1‑150 (MQ=255)     ttGATGCCGTCCAGCATTGGCAGCCGCTCGCGTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTAc                                                                                                                                         >  2:705457/2‑150 (MQ=255)            cGTCCAGCATTGGCAGCCGCTCGCGTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAg                                                                                                                                  <  2:559234/150‑1 (MQ=255)            cGTCCAGCATTGGCAGCCGCTCGCGTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTAACTGTTCAGTGAGCCTTGGCCGTACGGCCCAg                                                                                                                                  >  1:559234/1‑150 (MQ=255)             gTCCAGCATTGGCAGCCGCTCGCGTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTGGGCCGTACGGCCAAgg                                                                                                                                 <  1:347710/150‑1 (MQ=255)                       ggCAGCCTCTCGCGTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTATTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACgca                                                                                                                       <  1:412311/150‑1 (MQ=255)                             cGCTCGCGTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTTGCCGTACGGCCCAGTCGCCCAGGCAGGAAgg                                                                                                                 >  2:394301/1‑149 (MQ=255)                                ccgaggaCGGCCTGCCACTGCTGTGGCGGGATCCGGTAACGCGCCAGAGTACGCTTGGGCCGCCAGCGCCTGAATGACCACATGCCGTTCGTTCTTTAGCTGTGCAGTGAGCCTTGGCCGTACGGCCCAGGCTCCCACGCAGCAAGCCga                                                                                                              <  2:116184/144‑1 (MQ=255)                                    gTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAat                                                                                                          >  1:524235/1‑150 (MQ=255)                                        ggCCTGCCATTGCTGTGGCGTGATCGGGGAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAatcatc                                                                                                      >  2:503446/1‑150 (MQ=255)                                            tGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCACCTACGCAGCAAGCCGAGAATCATCGGCa                                                                                                  <  1:794749/150‑1 (MQ=255)                                                    cTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACg                                                                                                                         <  1:323541/119‑1 (MQ=255)                                                    cTGTGGCGTGATCGGGTAACGCGCCAGAGGACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACg                                                                                                                         >  2:323541/1‑119 (MQ=255)                                                                 gggTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGc                                                                                             >  1:521168/1‑134 (MQ=255)                                                                 gggTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGc                                                                                             <  2:521168/134‑1 (MQ=255)                                                                                     cTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCCTCAGCGACAgg                                                         >  1:558364/1‑150 (MQ=255)                                                                                          gccgccAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGtgctg                                                    >  1:159581/1‑150 (MQ=255)                                                                                            cgccAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTgctgct                                                  >  2:369067/1‑150 (MQ=255)                                                                                               cAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTtga                                               <  2:575898/150‑1 (MQ=255)                                                                                                       gAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGc                                       >  2:507517/1‑150 (MQ=255)                                                                                                            aCCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGtt                                  <  1:126231/150‑1 (MQ=255)                                                                                                             ccACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTtgat                                              <  2:471246/137‑1 (MQ=255)                                                                                                             ccACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTtgat                                              >  1:471246/1‑137 (MQ=255)                                                                                                                caTGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAatcat                                                                                                       >  1:24016/1‑77 (MQ=255)                                                                                                                caTGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAatcat                                                                                                       <  2:24016/77‑1 (MQ=255)                                                                                                                    ccGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCgaaga                          >  1:352041/1‑150 (MQ=255)                                                                                                                          gttcTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGc                    <  2:159581/150‑1 (MQ=255)                                                                                                                           ttcTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGcc                   <  1:503446/150‑1 (MQ=255)                                                                                                                                 aCCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGt             <  2:352041/150‑1 (MQ=255)                                                                                                                                  ccTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTg            >  1:105678/1‑150 (MQ=255)                                                                                                                                   cTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGa           >  2:279732/1‑150 (MQ=255)                                                                                                                                   cTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGa           >  1:724823/1‑150 (MQ=255)                                                                                                                                   cTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGa           <  1:369067/150‑1 (MQ=255)                                                                                                                                    tGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCCCGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGAc          <  1:394301/150‑1 (MQ=255)                                                                                                                                     gTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGAcc         >  2:801569/1‑150 (MQ=255)                                                                                                                                       tCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGACCAg       >  1:461466/1‑150 (MQ=255)                                                                                                                                         aGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGACCAGgc     >  2:501841/1‑150 (MQ=255)                                                                                                                                           tGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTg                                 <  2:273238/120‑1 (MQ=255)                                                                                                                                           tGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTg                                 >  1:273238/1‑120 (MQ=255)                                                                                                                                            gcgCCTGGGCCGTACGGCCAAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGACCAGgccgc  >  1:751173/3‑150 (MQ=255)                                                                                                                                              gCCTGGGCCGTACGGCCAAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGgatgatga                                                                                >  1:76281/1‑70 (MQ=39)                                                                                                                                              gCCTGGGCCGTACGGCCAAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGgatgatga                                                                                <  2:76281/70‑1 (MQ=39)                                                                                                                                                cTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGa                                                             >  2:121276/1‑87 (MQ=255)                                                                                                                                                cTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGa                                                             <  1:121276/87‑1 (MQ=255)                                                                                                                                                 ttGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAAt                                          >  2:148157/1‑105 (MQ=255)                                                                                                                                                 ttGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAAt                                          <  1:148157/105‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                GTCGCTGATGCCGTCCAGCATTGGCAGCCGCTCGCGTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGACCAGGCCGC  >  NC_002947/626414‑626702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑