| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 626,572 | C→A | 9.0% | L208L (CTG→CTT) | dedA ← | inner membrane protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 626,572 | 0 | C | A | 9.0% | 132.2 / 2.0 | 44 | L208L (CTG→CTT) | dedA | inner membrane protein |
| Reads supporting (aligned to +/- strand): ref base C (22/18); new base A (2/2); total (24/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
CGTCCAGCATTGGCAGCCGCTCGCGTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGACCAGGCCGCCGACCCAAAGCAGCGAACC > NC_002947/626425‑626721 | cGTCCAGCATTGGCAGCCGCTCGCGTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAg < 2:559234/150‑1 (MQ=255)cGTCCAGCATTGGCAGCCGCTCGCGTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTAACTGTTCAGTGAGCCTTGGCCGTACGGCCCAg > 1:559234/1‑150 (MQ=255) gTCCAGCATTGGCAGCCGCTCGCGTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTGGGCCGTACGGCCAAgg < 1:347710/150‑1 (MQ=255) ggCAGCCTCTCGCGTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTATTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACgca < 1:412311/150‑1 (MQ=255) cGCTCGCGTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTTGCCGTACGGCCCAGTCGCCCAGGCAGGAAgg > 2:394301/1‑149 (MQ=255) ccgaggaCGGCCTGCCACTGCTGTGGCGGGATCCGGTAACGCGCCAGAGTACGCTTGGGCCGCCAGCGCCTGAATGACCACATGCCGTTCGTTCTTTAGCTGTGCAGTGAGCCTTGGCCGTACGGCCCAGGCTCCCACGCAGCAAGCCga < 2:116184/144‑1 (MQ=255) gTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAat > 1:524235/1‑150 (MQ=255) ggCCTGCCATTGCTGTGGCGTGATCGGGGAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAatcatc > 2:503446/1‑150 (MQ=255) tGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCACCTACGCAGCAAGCCGAGAATCATCGGCa < 1:794749/150‑1 (MQ=255) cTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACg < 1:323541/119‑1 (MQ=255) cTGTGGCGTGATCGGGTAACGCGCCAGAGGACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACg > 2:323541/1‑119 (MQ=255) gggTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGc < 2:521168/134‑1 (MQ=255) gggTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGc > 1:521168/1‑134 (MQ=255) cTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCCTCAGCGACAgg > 1:558364/1‑150 (MQ=255) gccgccAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGtgctg > 1:159581/1‑150 (MQ=255) cgccAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTgctgct > 2:369067/1‑150 (MQ=255) cAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTtga < 2:575898/150‑1 (MQ=255) gAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGc > 2:507517/1‑150 (MQ=255) aCCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGtt < 1:126231/150‑1 (MQ=255) ccACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTtgat < 2:471246/137‑1 (MQ=255) ccACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTtgat > 1:471246/1‑137 (MQ=255) caTGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAatcat > 1:24016/1‑77 (MQ=255) caTGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAatcat < 2:24016/77‑1 (MQ=255) ccGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCgaaga > 1:352041/1‑150 (MQ=255) gttcTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGc < 2:159581/150‑1 (MQ=255) ttcTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGcc < 1:503446/150‑1 (MQ=255) aCCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGt < 2:352041/150‑1 (MQ=255) ccTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTg > 1:105678/1‑150 (MQ=255) cTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGa < 1:369067/150‑1 (MQ=255) cTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGa > 2:279732/1‑150 (MQ=255) cTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGa > 1:724823/1‑150 (MQ=255) tGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCCCGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGAc < 1:394301/150‑1 (MQ=255) gTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGAcc > 2:801569/1‑150 (MQ=255) tCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGACCAg > 1:461466/1‑150 (MQ=255) aGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGACCAGgc > 2:501841/1‑150 (MQ=255) tGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTg > 1:273238/1‑120 (MQ=255) tGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTg < 2:273238/120‑1 (MQ=255) gcgCCTGGGCCGTACGGCCAAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGACCAGgccgc > 1:751173/3‑150 (MQ=255) gCCTGGGCCGTACGGCCAAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGgatgatga < 2:76281/70‑1 (MQ=39) gCCTGGGCCGTACGGCCAAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGgatgatga > 1:76281/1‑70 (MQ=39) cTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGa > 2:121276/1‑87 (MQ=255) cTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGa < 1:121276/87‑1 (MQ=255) ttGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAAt > 2:148157/1‑105 (MQ=255) ttGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAAt < 1:148157/105‑1 (MQ=255) cccAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCtt < 2:168012/86‑1 (MQ=255) cccAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCtt > 1:168012/1‑86 (MQ=255) cccAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCAACAGCGAAAGGAAGATGATGCACACAAATTTCCGCCACAGGTGTTGCTGGACGAATTTCACGTTGCCGAAAAAGTAGCCCAGGGTGACCAGGCCGCCGACCCAAAGCAGCGaa < 2:524235/150‑1 (MQ=255) ccAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGACCAGGCCGCCGACCCAAAGCAGCGAAc < 1:279732/150‑1 (MQ=255) cAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGACCAGGCCGCCGACCCAAAGCAGCGAAcc < 2:105678/150‑1 (MQ=255) | CGTCCAGCATTGGCAGCCGCTCGCGTACGGCCTGCCATTGCTGTGGCGTGATCGGGTAACGCGCCAGAGTACGCTTGCGCCGCCAGGCGCTGAATGACCACATGCCGTTCGTTCTTTACCTGTTCAGTGAGCCTTGGCCGTACGGCCCAGGCGCCCACGCAGCAAGCCGAGAATCATCGGCACCAGCGACAGGATGATGATGCCCACCACCATCAGCGACAGGTGCTGCTTGATGAATGGCACGTTGCCGAAGAAGTAGCCCAAGGTGACCAGGCCGCCGACCCAAAGCAGCGAACC > NC_002947/626425‑626721 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |