| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 644,143 | G→C | 21.5% | R293G (CGC→GGC) | acoA ← | acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 644,143 | 0 | G | C | 21.5% | 81.9 / 22.4 | 42 | R293G (CGC→GGC) | acoA | acetoin:2,6‑dichlorophenolindophenol oxidoreductase subunit alpha |
| Reads supporting (aligned to +/- strand): ref base G (21/12); new base C (6/3); total (27/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.29e-01 | |||||||||||
ATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTCGAAA > NC_002947/643995‑644285 | aTCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCgg > 2:800084/1‑150 (MQ=255)aTCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCgg < 1:188708/150‑1 (MQ=255)aTCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCgg < 2:360399/150‑1 (MQ=255)aTCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCgg < 2:269137/150‑1 (MQ=255) ttcttGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGt < 2:361065/150‑1 (MQ=255) tGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGAt < 2:276015/150‑1 (MQ=255) tGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCCCGCGTCGag < 2:810983/150‑2 (MQ=255) ccATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATgg > 1:337836/1‑150 (MQ=255) cATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGc < 1:401873/150‑1 (MQ=255) ttGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGc > 1:14204/1‑150 (MQ=255) ttGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTAGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTAGCGCGGCGAACGGCGTCTTCGATAAGGTCCTCAACGCGGGCGTCGGTGGCGTCAAGTTGCTCGGCCGTGAGc > 1:330800/1‑150 (MQ=255) tCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCg > 1:380079/1‑150 (MQ=255) tCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCg > 2:189540/1‑150 (MQ=255) aGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCATCGACGCCCGCGTCGaggacc < 1:117104/113‑6 (MQ=255) aGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCATCGACGCCCGCGTCGaggacc > 2:117104/1‑108 (MQ=255) ggggTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGggtggt < 2:380079/150‑1 (MQ=255) caccGACGTCTACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTc < 2:219350/115‑1 (MQ=255) gACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTg > 1:613865/1‑150 (MQ=255) cGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCa < 2:613865/150‑1 (MQ=255) gTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGACGACTTCGATCAGGTCCTCGACGTGGGCGTCGATGGCGTCCAGTTGCTCGAACGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCAt > 2:344816/1‑150 (MQ=255) ggTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCCCGCGTCGagg < 1:421430/88‑3 (MQ=255) ggTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCCCGCGTCGagg > 2:421430/1‑86 (MQ=255) aGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGc < 2:286674/93‑1 (MQ=255) aGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGc > 1:286674/1‑93 (MQ=255) gTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTcgcg > 2:443281/1‑150 (MQ=255) gTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAAGCAGTcgcg > 1:391876/1‑150 (MQ=255) gcggGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGAt > 1:397244/1‑150 (MQ=255) tgtgGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCgcgc > 1:533712/1‑150 (MQ=255) tgGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCa > 1:286659/1‑132 (MQ=255) tgGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCa < 2:286659/132‑1 (MQ=255) gggATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGttctt > 2:260914/1‑150 (MQ=255) gcgAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGACGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGcgcg > 1:245194/1‑150 (MQ=255) aaCGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCAt > 1:383469/1‑99 (MQ=255) aaCGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCAt < 2:383469/99‑1 (MQ=255) ggCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGTGTAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGt < 2:330800/150‑1 (MQ=255) ttCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTgcgc > 2:191396/1‑150 (MQ=255) ttCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTgcgc > 2:190805/1‑150 (MQ=255) gccatCGACGCGCGCGTCGATCGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGGAGGTTTGCGCATCGCCTTc > 2:316029/5‑150 (MQ=255) tCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCtt < 2:394924/148‑1 (MQ=255) tCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCtt > 1:394924/1‑148 (MQ=255) ccatCGACGCCCGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTCg > 2:633821/4‑150 (MQ=255) catCGACGCCCGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTCGa > 1:90166/3‑150 (MQ=255) catCGACGCCCGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTCGa > 1:762545/3‑150 (MQ=255) atCGACGCCCGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGGGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTCGaa > 1:251309/2‑150 (MQ=255) tCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTCGaaa < 2:533712/150‑1 (MQ=255) tCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTCGaaa > 1:167728/1‑150 (MQ=255) | ATCTTTCTTGCCATGGTGGTTCTCCTGTTGTTCTTGTAATCCGCGGCTCAGGGGTAGGCGACGTAGACGTCGGTGAGCAGGTCGGCGGGCTGTGGCTTGGGATCGGATTTGGCGCGGCGAACGGCGTCTTCGATCAGGTCCTCGACGCGGGCGTCGATGGCGTCCAGTTGCTCGGCCGTGAGCAGGCCGGCGCGGGTGGTCTTGTTGCGAAATTGCATGAGGCAGTCGCGGGATTCGCGCAGGTTCTTCACTTCATCAGGGGCGCGGTAGGTTTGCGCATCGCCTTCGAAA > NC_002947/643995‑644285 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |