| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 58,750 | T→A | 18.9% | intergenic (‑661/+27) | PP_5424 ← / ← PP_0050 | hypothetical protein/hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 58,750 | 0 | T | A | 18.9% | 83.9 / 15.1 | 37 | intergenic (‑661/+27) | PP_5424/PP_0050 | hypothetical protein/hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base T (16/14); new base A (4/3); total (20/17) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.35e-01 | |||||||||||
GTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGGCGCGCTTTCCAGT > NC_002947/58605‑58898 | gTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCgg > 2:351212/1‑150 (MQ=255) tAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCAGCAGCggg > 1:493554/1‑150 (MQ=255) tttCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCttttt < 1:351212/150‑1 (MQ=255) tttCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCAGCAGCGGGCttttt < 2:493554/150‑1 (MQ=255) ttCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCaa > 2:417126/1‑150 (MQ=255) cTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAAc < 1:417126/150‑1 (MQ=255) gtgAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACaa > 1:260880/1‑150 (MQ=255) aGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTa < 2:260880/150‑1 (MQ=255) aCCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGcc > 2:666833/1‑150 (MQ=255) cacaAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGtt < 1:666833/150‑1 (MQ=255) acaAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTc > 1:505795/1‑150 (MQ=255) aaaaaaaaTGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCAc < 2:505795/150‑1 (MQ=255) aaaaaaaaTGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGAAACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTAAc > 2:828438/1‑150 (MQ=255) gAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATca < 2:140939/143‑1 (MQ=255) gAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATca > 1:140939/1‑143 (MQ=255) ttCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCaa < 2:24782/150‑1 (MQ=255) tCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAAt > 2:448711/1‑150 (MQ=255) aCCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACAAGAACATAGTCGCCATTca > 2:298560/1‑150 (MQ=255) cTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTcaca > 1:680296/1‑150 (MQ=255) cTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTcaca < 1:448711/150‑1 (MQ=255) cTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTcaca < 1:518465/150‑1 (MQ=255) tgtgAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGc < 2:504427/150‑1 (MQ=255) cTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACgat > 2:368212/1‑150 (MQ=255) tACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACgatg > 2:298993/1‑150 (MQ=255) aCAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGcc < 1:839108/56‑1 (MQ=255) aCAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGcc > 2:839108/1‑56 (MQ=255) aCAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTgg > 2:647669/1‑103 (MQ=255) aCAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTgg < 1:647669/103‑1 (MQ=255) ggTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCAc < 1:610005/150‑1 (MQ=255) gtAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAg < 2:680296/150‑1 (MQ=255) caggcacTAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCTCCATTc < 2:224181/112‑1 (MQ=38) cAGGCACTAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCTCCATTc > 1:224181/1‑116 (MQ=38) tAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTg < 1:834866/137‑1 (MQ=255) tAAAAAGCCCGCAGCAGCGGGCTTTTTAGTGTCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTg > 2:834866/1‑137 (MQ=255) aaaaaGCCCGCAGCAGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTgg > 2:742796/1‑150 (MQ=255) aaGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGgcgc > 2:793869/1‑150 (MQ=255) aaGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGgcgc > 1:222578/1‑150 (MQ=255) tgctgcGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGGCGCGCTTTCca > 1:688361/1‑150 (MQ=255) ctgcGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGGCGCGCTTTCcagt < 2:222578/150‑1 (MQ=255) | GTAGGGATTTCTCGGCTTTTCTGTGAAAAGCACCCCTTCATTTCACAAAAAAAATGGCGAAGATTCCAATTTCACGGGGTCTGGCCTATCACCTATTTTGTGAACCTACAAGGTGTAATTCGCCAGACACTAAAAAGCCCGCTGCTGCGGGCTTTTTAGTGCCTGCAAACAATCAGTAGCCGGTGAGAATGTTCACGATCACACCGCTGGCAATGGCCACCAGAACATAGTCGCCATTCACATACAGCCAACGATGATCACGAGGTGGCGCATACAGGTGGCGCGCTTTCCAGT > NC_002947/58605‑58898 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |