Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 646,275 T→A 11.1% intergenic (‑178/‑106) PP_0556 ← / → acoR acetoin catabolism protein/acetoin catabolism regulatory protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947646,2750TA11.1% 124.4 / 7.0 45intergenic (‑178/‑106)PP_0556/acoRacetoin catabolism protein/acetoin catabolism regulatory protein
Reads supporting (aligned to +/- strand):  ref base T (17/23);  new base A (3/2);  total (20/25)
Fisher's exact test for biased strand distribution p-value = 6.42e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.15e-01

GCAGGAGCAAGCGGGGTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGCCCATGTGGACTGCGTCAGCAG  >  NC_002947/646126‑646424
                                                                                                                                                     |                                                                                                                                                     
gCAGGAGCAAGCGGGGTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCt                                                                                                                                                       <  2:532103/150‑1 (MQ=255)
 cAGGAGCAAGCGGGGTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGACCGCGTTGCCGAGACACTGAGACGCTg                                                                                                                                                      <  1:204077/150‑1 (MQ=255)
  aGGAGCAAGCGGGGTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGc                                                                                                                                                     <  1:445636/150‑1 (MQ=255)
    gAGCAAGCGGGGTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGt                                                                                                                                                   <  2:510648/150‑1 (MQ=255)
          gCGGGGTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCAGCGTCTCagt                                                                                                                                             <  2:171314/150‑3 (MQ=255)
             gggTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTAGTGGTTGTTCAGGTTGTTGCGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAagcca                                                                                                                                          <  1:316954/150‑1 (MQ=255)
               gTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGAGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAagccagc                                                                                                                                        >  1:827542/1‑150 (MQ=255)
                  ccAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGt                                                                                                                                                   >  2:653665/1‑136 (MQ=255)
                  ccAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGt                                                                                                                                                   <  1:653665/136‑1 (MQ=255)
                    aGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGGTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTa                                                                                                                                   >  2:226627/1‑150 (MQ=255)
                       tttCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATc                                                                                                                                <  2:596427/150‑1 (MQ=255)
                           ttcGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATaacac                                                                                                                            >  2:842395/1‑150 (MQ=255)
                               cgATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTg                                                                                                                        <  1:556698/150‑1 (MQ=255)
                                   ggCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCt                                                                                                                                                       >  2:462250/1‑115 (MQ=255)
                                   ggCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCt                                                                                                                                                       <  1:462250/115‑1 (MQ=255)
                                     cTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTc                                                                                                                  <  1:639084/150‑1 (MQ=255)
                                        aaGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCg                                                                                                                                      <  2:465282/127‑1 (MQ=255)
                                        aaGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCg                                                                                                                                      <  2:37854/127‑1 (MQ=255)
                                        aaGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCg                                                                                                                                      >  1:465282/1‑127 (MQ=255)
                                        aaGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCg                                                                                                                                      >  1:37854/1‑127 (MQ=255)
                                          gTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACCGTGCTTGTCGGGGc                                                                                                             >  1:533353/1‑150 (MQ=255)
                                             gCCGGGCTAGAGGGCTCCGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATg                                                                                                          >  2:520072/1‑150 (MQ=255)
                                                        gggCTCTGGGCGTGGTCGGGCAGGTTTGCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGgcgc                                                                                               <  2:35236/150‑1 (MQ=255)
                                                              tGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATg                                                                                         <  1:848751/150‑1 (MQ=255)
                                                                gTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAagccag                                                                                                                                         >  1:500956/1‑100 (MQ=255)
                                                                gTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAagccag                                                                                                                                         <  2:500956/100‑1 (MQ=255)
                                                                gTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGc                                                                                       <  1:520072/150‑1 (MQ=255)
                                                                 tCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGcc                                                                                      >  2:100511/1‑150 (MQ=255)
                                                                 tCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGcc                                                                                      <  2:827542/150‑1 (MQ=255)
                                                                           gCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGAt                                                                            <  2:616537/150‑1 (MQ=255)
                                                                               gTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCt                                                                                                                                                       <  1:565712/71‑1 (MQ=255)
                                                                               gTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCt                                                                                                                                                       >  2:565712/1‑71 (MQ=255)
                                                                                     aCGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCNGCGTTGNCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCGTAATGGCCGGACAACGATAAAAACAGTa                                                                  >  1:618181/1‑150 (MQ=255)
                                                                                       gggTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCt                                                                                                                                                       >  1:592869/1‑63 (MQ=255)
                                                                                       gggTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCt                                                                                                                                                       <  2:592869/63‑1 (MQ=255)
                                                                                              gTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAAcc                                                         >  2:836024/1‑150 (MQ=255)
                                                                                              gTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAAcc                                                         >  2:836018/1‑150 (MQ=255)
                                                                                                   gagaCGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAgg                                                    >  2:680872/1‑150 (MQ=255)
                                                                                                       cGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCt                                                <  1:100511/150‑1 (MQ=255)
                                                                                                              tGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGACGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCt                                         <  2:117511/150‑1 (MQ=255)
                                                                                                                 cAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGc                                      <  1:226627/150‑1 (MQ=255)
                                                                                                                      ccGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGcc                                                                                      <  2:284242/97‑1 (MQ=255)
                                                                                                                      ccGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGcc                                                                                      >  1:284242/1‑97 (MQ=255)
                                                                                                                              gCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGa                         <  2:795846/150‑1 (MQ=255)
                                                                                                                                      gagaCACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACa                                                                     <  2:594627/98‑1 (MQ=255)
                                                                                                                                      gagaCACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACa                                                                     >  1:594627/1‑98 (MQ=255)
                                                                                                                                          gcttGAGACGCAGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGACCCCATGTGGAc             >  2:351901/4‑150 (MQ=255)
                                                                                                                                          cacTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGAAAACGATAAAAACAGTAACGAGAGCCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGCCCATGTGGAc             <  1:445861/150‑1 (MQ=255)
                                                                                                                                           cttGAGACGCAGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCCAACTCCCGAGCCCATGTGGACt            >  2:61141/3‑150 (MQ=255)
                                                                                                                                           acTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGAAGTGCGTGAAGGCTTGCCGCGAACTCCCGAGCCCATGTAGACt            >  2:300933/1‑150 (MQ=255)
                                                                                                                                            ttGAGACGCAGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTaa                                                                                           >  1:840700/2‑70 (MQ=255)
                                                                                                                                            ttGAGACGCAGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTaa                                                                                           <  2:840700/69‑1 (MQ=255)
                                                                                                                                                     tGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGAt                                                                            >  1:320864/1‑76 (MQ=255)
                                                                                                                                                     tGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGAt                                                                            <  2:320864/76‑1 (MQ=255)
                                                                                                                                                     tGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACAGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGCCCATGTGGACTGCGTcagcag  >  2:748349/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCAGGAGCAAGCGGGGTGCCAGCTTTCTTCGCGATGGCTGAAGTGGCCGGGCTAGAGGGCTCTGGTCGTGTTCGGGCAGGTTTTCACGGGTAGGGTGGCGAGACGCCGGGTGCCAGCGCCGGGCCTGCGTTGCCGAGACACTGAGACGCTGCGTCTCAAGCCAGCCGGTAATCACACAGTGCTTGTCGGGGCATGCCCAGAGGCGCTTAATGGCCGGACAACGATAAAAACAGTAACGAGAACCGGAGGGCCTGAATGCTTGCCGCGAACTCCCGAGCCCATGTGGACTGCGTCAGCAG  >  NC_002947/646126‑646424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑