| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 654,406 | T→G | 8.8% | K114Q (AAG→CAG) | ctpL ← | methyl‑accepting chemotaxis protein CtpL |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 654,406 | 0 | T | G | 8.8% | 116.3 / 3.7 | 46 | K114Q (AAG→CAG) | ctpL | methyl‑accepting chemotaxis protein CtpL |
| Reads supporting (aligned to +/- strand): ref base T (23/17); new base G (2/2); total (26/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.49e-01 | |||||||||||
GGTGGCGTCTAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGGCGCAGGGCGTCGC > NC_002947/654266‑654555 | ggTGGCGTCTAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCGCCCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGCCGCCGGCAAGCTGGCcggcga < 2:374000/150‑2 (MQ=255) cGTCTAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCcagca < 2:427918/150‑1 (MQ=255) cGTCTAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCcagca < 1:323897/150‑1 (MQ=255) cGTCTAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTCGCCGGCGGCcagca > 1:1453/1‑150 (MQ=255) gTCTAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGCCGCCGGCAAGCTGGCcggcgaccca < 1:277530/147‑6 (MQ=255) gTCTAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGCCGCCGGCAAGCTGGCcggcgaccca > 2:277530/1‑142 (MQ=255) tAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTc < 2:778414/150‑1 (MQ=255) gCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCtt < 2:205861/130‑1 (MQ=255) gCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCtt > 1:205861/1‑130 (MQ=255) gCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGt > 2:322555/1‑150 (MQ=255) ggCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCCGTTCGTTGGCg > 2:147050/1‑150 (MQ=255) caAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGaa > 1:613297/1‑150 (MQ=255) aaGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTCGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAg < 2:1453/150‑1 (MQ=255) gCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTg > 2:16103/1‑150 (MQ=255) cTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATc > 2:824005/1‑150 (MQ=255) cGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGTGCGTGTTGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCGTGCAGGCTATCCa > 2:330878/1‑150 (MQ=255) gCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTgg > 1:74176/1‑149 (MQ=255) gCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTgg < 2:74176/149‑1 (MQ=255) cACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGgc > 2:127519/1‑150 (MQ=255) aCTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGgcg > 1:89646/1‑150 (MQ=255) cgGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGGCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTtc > 1:690173/1‑150 (MQ=255) cgGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGAAGGGCTTGGGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTtc > 2:420563/1‑150 (MQ=255) gCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCg < 1:741864/150‑1 (MQ=255) gCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGACGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCg < 2:613297/150‑1 (MQ=255) cGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCgg < 1:424113/150‑1 (MQ=255) tGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTg < 1:711483/98‑1 (MQ=255) tGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTg > 2:711483/1‑98 (MQ=255) cTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCa > 2:374519/1‑150 (MQ=255) cTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCa > 1:430650/1‑150 (MQ=255) gAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAgg < 1:174441/103‑1 (MQ=255) gAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAgg > 2:174441/1‑103 (MQ=255) gAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTc > 1:373206/1‑150 (MQ=255) gAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTAGCCGACCGGCTTGCCGGCGGCCAGCAGGTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTc > 1:531882/1‑150 (MQ=255) tCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCa < 1:824005/150‑1 (MQ=255) tCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCa > 1:382659/1‑150 (MQ=255) cccGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCa > 1:298966/1‑150 (MQ=255) cGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAgtc < 1:829644/94‑3 (MQ=255) cGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAgtc > 2:829644/1‑92 (MQ=255) ggCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTg < 1:374519/150‑1 (MQ=255) ggCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATggc < 2:382659/150‑1 (MQ=255) ggCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATggc > 2:502414/1‑150 (MQ=255) ctggccGCCGGCAAGCTGGCCGGCGACCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATggcggcggc > 1:251350/6‑150 (MQ=255) gggTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGcc < 2:373206/150‑1 (MQ=255) gTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGgc > 2:441278/1‑71 (MQ=255) gTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGgc < 1:441278/71‑1 (MQ=255) cccgCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGTCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGGCGCAggg > 2:643955/4‑150 (MQ=255) cAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGGCGCAGGGc < 1:330878/150‑1 (MQ=255) cTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGGCGCAGGGCGTc < 1:420563/150‑1 (MQ=255) tGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGGCGCAGGGCGTcgc < 2:91869/150‑1 (MQ=255) | GGTGGCGTCTAGCAAGGGCAGCAAGTAGCGATTGGCCTCGGCGTTGCCACTGTCGCGGGCGTAGCTGGCCATCTGCTCGAAGTTCG‑CCCCCAGCTCCCGCTCGGCTTGCAGCAGCAGGGCTTGTGGGTCGCCGGCCAGCTTGCCGGCGGCCAGCAGTTCGTTGGCGGTGAAGGCCTGCAGGCTATCCAGGCTGGGGCGCAACTTCTCGGCCAGCTCTACCGGCCAGTCCGTCAGTGCGGCCTGCAACTGTTGGTTCGCCTCCATGGCGGCGGCCTGGCGCAGGGCGTCGC > NC_002947/654266‑654555 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |