| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | NC_002947 | 699,725 | A→G | 45.7% | intergenic (+115/‑129) | PP_t06 → / → PP_23SD | tRNA‑Ala/23S ribosomal RNA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 699,725 | 0 | A | G | 45.7% | 2.5 / 41.0 | 35 | intergenic (+115/‑129) | PP_t06/PP_23SD | tRNA‑Ala/23S ribosomal RNA |
| Reads supporting (aligned to +/- strand): ref base A (10/9); new base G (7/9); total (17/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01 | |||||||||||
GAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCG > NC_002947/699577‑699808 | gaggTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGAc < 2:760480/150‑1 (MQ=39) tCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGaa < 2:441591/150‑1 (MQ=255) tCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGaa > 2:310470/1‑150 (MQ=255) cAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAac > 2:478279/1‑150 (MQ=255) cGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACAcc < 2:74522/150‑1 (MQ=38) ccGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCActg > 2:424794/1‑150 (MQ=39) cTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCActgctg < 1:310470/150‑1 (MQ=255) tGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCtggt < 1:73417/150‑1 (MQ=255) ggCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCtggtg < 1:424794/150‑1 (MQ=39) ccaccaCTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATc < 2:278207/150‑1 (MQ=255) caccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATGGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCa < 1:396779/150‑1 (MQ=255) accacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAg > 2:18146/1‑150 (MQ=255) ccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAgg < 1:668121/150‑1 (MQ=255) ccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAgg > 1:67097/1‑150 (MQ=39) ccacTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAgg > 2:793602/1‑150 (MQ=39) cTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTa > 2:356796/1‑150 (MQ=38) gCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGCTCAGGCTCAGGTAAAATTtg > 2:177288/1‑150 (MQ=255) cAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt < 2:843649/150‑1 (MQ=255) cAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt < 2:829252/150‑1 (MQ=255) cAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAACTtgt < 2:67097/150‑1 (MQ=37) tACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGt > 1:370233/1‑150 (MQ=35) ttGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCt > 2:357447/1‑150 (MQ=21) cAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt < 2:224380/139‑1 (MQ=37) cAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt > 1:224380/1‑139 (MQ=37) gAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt > 1:672692/1‑137 (MQ=37) gAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTtgt < 2:672692/137‑1 (MQ=37) aCTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGaaa < 2:705851/150‑1 (MQ=35) cTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAag > 2:279045/1‑150 (MQ=35) ttAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGGGGTGCTCGAAaga > 1:176989/1‑150 (MQ=255) caGAAATCAACATTCGAGCT‑GAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAagag > 1:638528/2‑150 (MQ=11) agtGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAAc < 1:219461/148‑1 (MQ=25) aTTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGGCTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGaa > 1:113448/1‑150 (MQ=25) ttC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCaa > 2:647863/1‑141 (MQ=34) ttC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCaa < 1:647863/141‑1 (MQ=34) cTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCg < 1:411762/150‑1 (MQ=11) | GAGGTCAGCGGTTCGATCCCGCTTGGCTCCACCACTTTCGCCGTACGCAGTTACTTGTCAGAACTTAGAAATGAACATTC‑AGCTCGAATGTTGATTTCTGGCTTTTGTCAGATCGTTCTTTAAAAATTCGGATATGTGATAGATATAGACTGAACACCAGTTTCACTGCTGGTGGATCAGGCTAAGGTAAAATTTGTGAGTTCTGCTCGAAAGAGCAACGTACGAATTTTCG > NC_002947/699577‑699808 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |