Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	772,182	T→G	13.8%	intergenic (+40/‑159)	PP_0663 → / → PP_0664	AsnC family transcriptional regulator/homoserine dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	772,182	0	T	G	13.8%	62.7 / 5.2	29	intergenic (+40/‑159)	PP_0663/PP_0664	AsnC family transcriptional regulator/homoserine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (13/12);  new base G (2/2);  total (15/14)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.72e-01

CGAGGCCTCCAGCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGCCTGGAC  >  NC_002947/772037‑772327                                                                                                                                                  |                                                                                                                                                  cGAGGCCTCCAGCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTcgcg                                                                                                                                               <  2:228194/150‑1 (MQ=255)      ccTCCAGCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAg                                                                                                                                          >  1:772494/1‑150 (MQ=255)                           aCCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTtgt                                                                                                                                >  2:284049/1‑139 (MQ=255)                           aCCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTtgt                                                                                                                                <  1:284049/139‑1 (MQ=255)                            ccATGTGTGGAAGGAAATCGCTGCCATGCCCTGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCt                                                                                                                    >  1:710074/1‑150 (MQ=255)                                 tgtgGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGgcgc                                                                                                               >  2:517791/1‑150 (MQ=255)                                  gtgGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTTGTCTGCACCGCGGCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCACTgacaca                                                                                                              >  1:274532/1‑145 (MQ=255)                                     gaaggaaATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGtt                                                                                                                                  >  2:651017/1‑127 (MQ=255)                                     gaaggaaATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGtt                                                                                                                                  <  1:651017/127‑1 (MQ=255)                                     gaaggaaATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGCGCGAACCCAGGCGGCGtt                                                                                                                                  <  2:256937/127‑1 (MQ=38)                                     gaaggaaATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGCGCGAACCCAGGCGGCGtt                                                                                                                                  >  1:256937/1‑127 (MQ=38)                                     gaaggaaAACGCTGACATGCTCGAAGTCTAGAACACGGAGGCCCAGGTCGTCCTGGCCGCGGTGCACTGAACCGCGTCGACCCATTAGCTAGCACAAAGCCGCCTCGGGTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGAACAGGt                                                                                                           >  2:65653/1‑150 (MQ=255)                                           aaTCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTgcgcgc                                                                                                     >  2:221806/1‑150 (MQ=255)                                                 tGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGCTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCa                                                                                               >  2:151968/1‑150 (MQ=255)                                                   ccATGCCTGGGGTGGAGAAGACGGTGACTTCCTTCGTCCTGGCCGCGGTTGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACt                                                                                             <  2:274532/150‑1 (MQ=255)                                                   ccATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCa                                                                                                                                           >  1:785799/1‑104 (MQ=255)                                                   ccATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCa                                                                                                                                           <  2:785799/104‑1 (MQ=255)                                                    cATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTg                                                                                            <  2:707622/150‑1 (MQ=255)                                                     aTGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGCGCGAACCCAGGCGGCGTTGTGctcgc                                                                                                                          >  1:215177/1‑115 (MQ=37)                                                     aTGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGCGCGAACCCAGGCGGCGTTGTGctagc                                                                                                                          <  2:215177/119‑5 (MQ=37)                                                     aTGCCCGGGGTGGAGAACACGGCGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTgg                                                                                           >  1:630947/1‑150 (MQ=255)                                                               tGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGc                                                                                 <  2:710074/150‑1 (MQ=255)                                                                  agaACACGGCGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCaa                                                                              <  2:630947/150‑1 (MQ=255)                                                                                     cgtCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCa                                                           <  1:221806/150‑1 (MQ=255)                                                                                         cTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATAtt                                                       >  1:651486/1‑150 (MQ=255)                                                                                            gCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCACATTCaa                                                    <  2:595143/150‑1 (MQ=255)                                                                                             ccGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGTTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAAc                                                   <  2:303189/150‑1 (MQ=255)                                                                                                                   tttGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGc                             >  1:569558/1‑150 (MQ=255)                                                                                                                                              gggTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGCCTggac  <  2:569558/150‑1 (MQ=255)                                                                                                                                                 ttCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAAc                                                   >  2:203206/1‑98 (MQ=255)                                                                                                                                                 ttCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAAc                                                   <  1:203206/98‑1 (MQ=255)                                                                                                                                                  |                                                                                                                                                  CGAGGCCTCCAGCCCGGAGCGGGTGCACCATGTGTGGAAGGAAATCGCTGCCATGCCCGGGGTGGAGAACACGGTGACTTCGTTCGTCCTGGCCGCGGTGGTCTGAACCGCGTCTTTGCATTGGCTAGCACAACGCCGCCTGGGTTCGCGCCCAGGCGGCGTTGTGCGTTTTGCCCTGGCGCAGGTGCGCGCTTTGCACTGGCCAGATTGGCCAATAAATACAGCGGATTGCCATATTCAACTGGCAGTGCACGGCTCTTACGCTAGTCCTCATCGACCCACTGCCTGGAC  >  NC_002947/772037‑772327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑