Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 5,914,046 +A coding (1259/1377 nt) spuI → glutamylpolyamine synthetase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,914,0441.A99.8% 1510.8 / ‑4.0 415coding (1257/1377 nt)spuIglutamylpolyamine synthetase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base A (184/230);  total (184/231)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

Only 100 of 442 total aligned reads displayed.

GCTGCCGTACTGGCTGGCGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTG‑AACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGG  >  NC_002947/5913895‑5914186
                                                                                                                                                      |                                                                                                                                              
  tGCCGTACTGGCTGGCGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGATAGCGAGATCCTG‑aa                                                                                                                                              >  2:7115430/1‑150 (MQ=255)
  tGCCGTACTGGCTGGCGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTG‑aa                                                                                                                                              >  2:3760930/1‑150 (MQ=255)
  tGCCGTACTGGCTGGCGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTG‑aa                                                                                                                                              >  1:3074816/1‑150 (MQ=255)
      gTACTGGCTGGCGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTG‑aaacaa                                                                                                                                          <  2:6546877/150‑5 (MQ=255)
      gTACTGGCTGGCGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTG‑aaacaa                                                                                                                                          <  2:4822228/150‑5 (MQ=255)
      gTACTGGCTGGCGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTG‑aaacaa                                                                                                                                          <  2:1162436/150‑5 (MQ=255)
        aCTGGCTGGCGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGt                                                                                                                                         <  2:4561461/150‑1 (MQ=255)
         ctggctggCGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTa                                                                                                                                        >  2:6454345/1‑150 (MQ=255)
          tggctggcGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTCCGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCGGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTAc                                                                                                                                       >  1:4648518/1‑150 (MQ=255)
          tggctggcGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTAc                                                                                                                                       <  1:3405436/150‑1 (MQ=255)
          tggctggcGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTAc                                                                                                                                       >  1:9839491/1‑150 (MQ=255)
             ctggcGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATc                                                                                                                                    <  1:9538437/150‑1 (MQ=255)
              tggcGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCg                                                                                                                                   >  1:6898802/1‑150 (MQ=255)
              tggcGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCg                                                                                                                                   >  1:1778937/1‑150 (MQ=255)
                     cACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGaa                                                                                                                            >  2:3255530/1‑150 (MQ=255)
                     cACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGaa                                                                                                                            <  2:7502991/150‑1 (MQ=255)
                      aCCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAg                                                                                                                           >  2:2677884/1‑150 (MQ=255)
                        cATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACGAGTACAGCGCTCCGAAGtc                                                                                                                         >  2:10148552/1‑149 (MQ=18)
                        cATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTa                                                                                                                         >  1:5423833/1‑150 (MQ=255)
                        cATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTa                                                                                                                         >  1:4211672/1‑150 (MQ=255)
                        cATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTa                                                                                                                         >  2:552193/1‑150 (MQ=255)
                        cATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTa                                                                                                                         <  1:4592251/150‑1 (MQ=255)
                         aTGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTAc                                                                                                                        <  2:1768506/150‑1 (MQ=255)
                          tGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACa                                                                                                                       <  2:617246/150‑1 (MQ=255)
                          tGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACa                                                                                                                       >  2:7704595/1‑150 (MQ=255)
                           ggCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACAt                                                                                                                      <  1:5529528/150‑1 (MQ=255)
                            gCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATc                                                                                                                     <  2:528437/150‑1 (MQ=255)
                            gCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATc                                                                                                                     <  2:8799474/150‑1 (MQ=255)
                               tGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGAc                                                                                                                  <  1:7221953/150‑1 (MQ=255)
                                gACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACa                                                                                                                 <  1:5104127/150‑1 (MQ=255)
                                    aacaaGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCtt                                                                                                             >  1:407501/1‑150 (MQ=255)
                                    aacaaGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCtt                                                                                                             >  1:9271879/1‑150 (MQ=255)
                                    aacaaGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCtt                                                                                                             >  1:7554211/1‑150 (MQ=255)
                                     acaaGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGTACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTtc                                                                                                            <  2:9927847/150‑1 (MQ=255)
                                     acaaGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTtc                                                                                                            >  2:1689060/1‑150 (MQ=255)
                                      caaGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTtcg                                                                                                           >  2:1957336/1‑150 (MQ=255)
                                              aGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGc                                                                                                   <  2:8517636/150‑1 (MQ=255)
                                              aGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGACGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGc                                                                                                   >  1:8218412/1‑150 (MQ=255)
                                                ccAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCaa                                                                                                 <  2:1396758/150‑1 (MQ=255)
                                                ccAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCaa                                                                                                 >  1:3298703/1‑150 (MQ=255)
                                                 cAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAg                                                                                                <  1:62104/150‑1 (MQ=255)
                                                   ggCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGa                                                                                              <  1:1653107/150‑1 (MQ=255)
                                                     cgcgcCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGaaa                                                                                            <  1:1470904/150‑1 (MQ=255)
                                                         ccGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGa                                                                                        <  1:3730546/150‑1 (MQ=255)
                                                           gATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGc                                                                                      <  2:9698104/150‑1 (MQ=255)
                                                            gtCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCt                                                                                     >  1:10012409/2‑150 (MQ=255)
                                                              cGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTgg                                                                                   <  2:7207315/150‑1 (MQ=255)
                                                               gAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGa                                                                                  <  1:4815403/150‑1 (MQ=255)
                                                                aaGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGaa                                                                                 <  1:8903280/150‑1 (MQ=255)
                                                                aaGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGaa                                                                                 <  2:10003964/150‑1 (MQ=255)
                                                                aaGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGaa                                                                                 <  1:3128779/150‑1 (MQ=255)
                                                                aaGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGaa                                                                                 <  1:9960968/150‑1 (MQ=255)
                                                                aaGCCAACTAGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTCCGCGATGCCCTGCTCGAGCAGGACGACAGCGAGATCATGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGaa                                                                                 <  2:214788/150‑1 (MQ=255)
                                                                 aGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAag                                                                                >  1:4411271/1‑150 (MQ=255)
                                                                 aGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAag                                                                                >  2:3418160/1‑150 (MQ=255)
                                                                  ggCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAaga                                                                               <  2:2879491/150‑1 (MQ=255)
                                                                   gCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAagag                                                                              >  2:4155167/1‑150 (MQ=255)
                                                                   gCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAagag                                                                              >  2:9285410/1‑150 (MQ=255)
                                                                   gCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAagag                                                                              >  2:4053305/1‑150 (MQ=255)
                                                                      aCTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTc                                                                           <  2:5673234/150‑1 (MQ=255)
                                                                      aCTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTc                                                                           <  1:573621/150‑1 (MQ=255)
                                                                        tCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGa                                                                         <  2:1584625/150‑1 (MQ=255)
                                                                        tCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGa                                                                         <  2:3066893/150‑1 (MQ=255)
                                                                          gTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGc                                                                       >  2:4641616/1‑150 (MQ=255)
                                                                               agcagcTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGc                                                                                          <  1:637935/126‑1 (MQ=255)
                                                                               agcagcTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCg                                                                  >  1:3991119/1‑150 (MQ=255)
                                                                                 cagcTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGAt                                                                >  1:9378756/1‑150 (MQ=255)
                                                                                     tGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTcc                                                            <  1:6414206/150‑1 (MQ=255)
                                                                                     tGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTcc                                                            >  1:6616457/1‑150 (MQ=255)
                                                                                         gCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGAcc                                                        >  1:8440296/1‑150 (MQ=255)
                                                                                          cAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCt                                                       >  1:5230763/1‑150 (MQ=255)
                                                                                           agagCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTc                                                      <  1:1982000/150‑1 (MQ=255)
                                                                                           agagCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTc                                                      >  2:9101998/1‑150 (MQ=255)
                                                                                              gCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAg                                                   <  1:9647733/150‑1 (MQ=255)
                                                                                              gCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAg                                                   <  1:7768855/150‑1 (MQ=255)
                                                                                              gCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAg                                                   <  1:7965196/150‑1 (MQ=255)
                                                                                               ccTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGt                                                  >  2:5159995/1‑150 (MQ=255)
                                                                                                 tGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCtggtggac                                                >  2:782388/1‑143 (MQ=255)
                                                                                                     gAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTtc                                                                                                            <  2:9401873/86‑1 (MQ=255)
                                                                                                     gAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACt                                            >  2:2466504/1‑150 (MQ=255)
                                                                                                      aacaacCTGCGCGATTCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTg                                           <  2:7391848/150‑1 (MQ=255)
                                                                                                         aacCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACCTCGACAACTTCGTCGCGTGCAAGGAACGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTa                                        >  1:7482486/1‑150 (MQ=37)
                                                                                                         aacCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTa                                        >  1:2578610/1‑150 (MQ=255)
                                                                                                           ccTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTAcc                                      >  1:1294008/1‑150 (MQ=255)
                                                                                                            cTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCt                                     >  2:5858281/1‑150 (MQ=255)
                                                                                                            cTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCt                                     <  1:9012885/150‑1 (MQ=255)
                                                                                                            cTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCt                                     >  2:4343289/1‑150 (MQ=255)
                                                                                                              gcgcGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGc                                   <  1:5876069/150‑1 (MQ=255)
                                                                                                                  gATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATAc                               >  1:8546378/1‑150 (MQ=255)
                                                                                                                          gcgcGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTaaa                       <  1:2774433/150‑1 (MQ=255)
                                                                                                                          gcgcGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTaaa                       <  1:114709/150‑1 (MQ=255)
                                                                                                                          gcgcGAGCTGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTaaa                       <  1:4493129/150‑1 (MQ=255)
                                                                                                                                  tGGACGACAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAAcg               <  2:1326824/150‑1 (MQ=255)
                                                                                                                                    gacgacAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAAcgcc             >  1:8708479/1‑150 (MQ=255)
                                                                                                                                    gacgacAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAAcgcc             <  1:1791524/150‑1 (MQ=255)
                                                                                                                                    gacgacAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAAcgcc             >  2:3621150/1‑150 (MQ=255)
                                                                                                                                      cgacAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAAcgccgc           <  2:6918199/150‑1 (MQ=255)
                                                                                                                                       gacAGCGAGATCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACgccgcc          >  1:403837/1‑150 (MQ=255)
                                                                                                                                             gagaTCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAAAGCCGCCCCTCaa    >  1:2268497/1‑150 (MQ=255)
                                                                                                                                              agaTCCTGAAACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCTATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAg   <  1:9699818/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                              
GCTGCCGTACTGGCTGGCGTGCACCATGGCCTGACCAACAAGATCGAGCCAGGCGCGCCGATCGAAGGCAACTCGTACGAGCAGCTGGAGCAGAGCCTGCCGAACAACCTGCGCGATGCCCTGCGCGAGCTGGACGACAGCGAGATCCTG‑AACAAGTACATCGATCCGAAGTACATCGACATCTTCGTCGCGTGCAAGGAAAGCGAGCTGGAAGAGTTCGAGCACTCGATCTCCGACCTCGAGTACAACTGGTACCTGCATACCGTGTAAACGAAAACGCCGCCCCTCAAGG  >  NC_002947/5913895‑5914186

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑