Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,843,140 G→A Q566* (CAG→TAG)  gacS ← sensor protein GacS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,843,1400GA99.4% 1153.7 / ‑3.9 324Q566* (CAG→TAG) gacSsensor protein GacS
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (178/143);  total (180/144)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.25e-01

Only 100 of 331 total aligned reads displayed.

TCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCTTCGGCAT  >  NC_002947/1842993‑1843286
                                                                                                                                                   |                                                                                                                                                  
  gggCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTacagc                                                                                                                                                >  1:1652445/1‑150 (MQ=255)
   ggCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTacagca                                                                                                                                               >  2:9289553/1‑150 (MQ=255)
        aGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTgg                                                                                                                                          >  1:6288106/1‑150 (MQ=255)
         gCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGt                                                                                                                                         <  2:236013/150‑1 (MQ=255)
             tACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTa                                                                                                                                     <  1:1066031/150‑1 (MQ=255)
             tACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTa                                                                                                                                     >  2:9188915/1‑150 (MQ=255)
             tACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTa                                                                                                                                     >  1:3688078/1‑150 (MQ=255)
              aCGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTag                                                                                                                                    <  1:2221816/150‑1 (MQ=255)
               cGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTagg                                                                                                                                   >  1:7472724/1‑150 (MQ=255)
               cGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTagg                                                                                                                                   <  2:6233448/150‑1 (MQ=255)
                gCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTagga                                                                                                                                  >  2:5287305/1‑150 (MQ=255)
                gCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTagga                                                                                                                                  >  1:8025962/1‑150 (MQ=255)
                 cATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTaggag                                                                                                                                 <  2:1344942/150‑1 (MQ=255)
                 cATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTaggag                                                                                                                                 <  1:9546878/150‑1 (MQ=255)
                      gCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGaa                                                                                                                            >  2:8776617/1‑150 (MQ=255)
                        aGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACa                                                                                                                          <  2:2653222/150‑1 (MQ=255)
                            gTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCt                                                                                                                      >  1:3100434/1‑150 (MQ=255)
                                 gCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGc                                                                                                                 >  2:9807173/1‑150 (MQ=255)
                                 gCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGc                                                                                                                 >  1:4522806/1‑150 (MQ=255)
                                 gCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGc                                                                                                                 >  1:3916111/1‑150 (MQ=255)
                                  cTCGAGCTGCTGATGAGAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCACCTGGTCGTAGGAGGCGAACAGGCTCACGCt                                                                                                                <  2:1588844/150‑1 (MQ=255)
                                    cGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGa                                                                                                              <  2:3815696/150‑1 (MQ=255)
                                    cGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGa                                                                                                              <  1:1564248/150‑1 (MQ=255)
                                    cGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGa                                                                                                              <  2:3212699/150‑1 (MQ=255)
                                    cGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGa                                                                                                              <  2:3420369/150‑1 (MQ=255)
                                     gAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAg                                                                                                             >  2:6658800/1‑150 (MQ=255)
                                           ctgATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCa                                                                                                       <  1:8264898/150‑1 (MQ=255)
                                           ctgATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCa                                                                                                       <  1:10084923/150‑1 (MQ=255)
                                              atgatAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTc                                                                                                    <  1:5792984/150‑1 (MQ=255)
                                                gatAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCtt                                                                                                  >  2:8430095/1‑150 (MQ=255)
                                                  tAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTc                                                                                                    >  2:3288647/1‑146 (MQ=255)
                                                  tAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTc                                                                                                    <  1:3288647/146‑1 (MQ=255)
                                                      ggCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCt                                                                                            <  2:7221432/150‑1 (MQ=255)
                                                       gCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTg                                                                                           >  2:7979769/1‑150 (MQ=255)
                                                         ccAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGt                                                                                         >  2:9718854/1‑150 (MQ=255)
                                                             ttGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTc                                                                                     >  1:1426800/1‑150 (MQ=255)
                                                             ttGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCACACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTc                                                                                     <  2:368254/150‑1 (MQ=255)
                                                               gCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCa                                                                                   >  2:5882250/1‑150 (MQ=255)
                                                               gCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCa                                                                                   <  1:5029686/150‑1 (MQ=255)
                                                                 tCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGt                                                                                 >  2:895185/1‑150 (MQ=255)
                                                                 tCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGt                                                                                 <  2:4147848/150‑1 (MQ=255)
                                                                 tCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGt                                                                                 >  1:9588945/1‑150 (MQ=255)
                                                                    ggCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGcc                                                                              <  2:9384614/150‑1 (MQ=255)
                                                                       gACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACGCGAAGGGCAGGCCGGCCTGGCAGGCGGCCTGCACCCCCTACAGCAACTGGACGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTgg                                                                           >  2:4898638/1‑150 (MQ=255)
                                                                          aGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGgcgc                                                                        <  1:3503494/150‑1 (MQ=255)
                                                                            gTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGgcgcgc                                                                      <  1:8146627/150‑1 (MQ=255)
                                                                            gTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGgcgcgc                                                                      >  2:2521416/1‑150 (MQ=255)
                                                                            gTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGgcgcgc                                                                      >  2:4580844/1‑150 (MQ=255)
                                                                             ttACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGAAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGcc                                                                     >  2:8368286/1‑150 (MQ=255)
                                                                              tACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCa                                                                    <  1:7713082/150‑1 (MQ=255)
                                                                                 ccAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGtt                                                                 >  1:9852595/1‑150 (MQ=255)
                                                                                 ccAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGtt                                                                 >  1:9440770/1‑150 (MQ=255)
                                                                                 ccAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGtt                                                                 >  1:7295553/1‑150 (MQ=255)
                                                                                 ccAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGtt                                                                 >  2:2119383/1‑150 (MQ=255)
                                                                                  cAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTc                                                                <  2:1102288/150‑1 (MQ=255)
                                                                                  cAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTc                                                                <  2:281270/150‑1 (MQ=255)
                                                                                   aGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCg                                                               >  2:7379407/1‑150 (MQ=255)
                                                                                     gTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTg                                                             <  1:2676977/150‑1 (MQ=255)
                                                                                      ttGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTgg                                                            >  1:8493665/1‑150 (MQ=255)
                                                                                      ttGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTgg                                                            >  2:426521/1‑150 (MQ=255)
                                                                                       tGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGc                                                           >  2:8513889/1‑150 (MQ=255)
                                                                                          cACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCAt                                                        >  2:552636/1‑150 (MQ=255)
                                                                                             cGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGa                                                     >  1:1819095/1‑150 (MQ=255)
                                                                                                 cACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAAt                                                 >  1:8547664/1‑150 (MQ=255)
                                                                                                 cACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAAt                                                 <  1:6737588/150‑1 (MQ=255)
                                                                                                  aCCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATg                                                >  1:7110894/1‑150 (MQ=255)
                                                                                                    cgcgAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATggc                                              >  1:8876544/1‑150 (MQ=255)
                                                                                                    cgcgAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATggc                                              >  1:9005002/1‑150 (MQ=255)
                                                                                                    cgcgAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATggc                                              <  1:3403832/150‑1 (MQ=255)
                                                                                                           tCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCAcgg                                       >  2:9890900/1‑150 (MQ=255)
                                                                                                             gAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCAcggcg                                     >  1:7629464/1‑150 (MQ=255)
                                                                                                             gAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCAcggcg                                     >  2:6462035/1‑150 (MQ=255)
                                                                                                              aaGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACATGCTCACGCTGAGGCCGCAGTCTTCCAGATGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCAcggcgg                                    <  1:6089635/150‑1 (MQ=255)
                                                                                                              aaGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCAcggcgg                                    >  1:9194685/1‑150 (MQ=255)
                                                                                                                gggCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGcc                                  >  1:5435215/1‑150 (MQ=255)
                                                                                                                  gCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCa                                <  1:8389161/150‑1 (MQ=255)
                                                                                                                   cAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAg                               <  2:9111724/150‑1 (MQ=255)
                                                                                                                    aGGCCGGCCTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGc                              >  2:1826921/1‑150 (MQ=255)
                                                                                                                     ggccggccTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCa                             >  1:9100112/1‑150 (MQ=255)
                                                                                                                       ccggccTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAAt                           <  2:6348195/150‑1 (MQ=255)
                                                                                                                       ccggccTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAAt                           <  2:2877680/150‑1 (MQ=255)
                                                                                                                        cggccTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATg                          >  1:2942412/1‑150 (MQ=255)
                                                                                                                         ggccTGGCTGGCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATgg                         <  2:5121769/150‑1 (MQ=255)
                                                                                                                            ctggctggCGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTg                      >  2:7119256/1‑150 (MQ=255)
                                                                                                                             tggctggcGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGc                     >  2:4976198/1‑150 (MQ=255)
                                                                                                                             tggctggcGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGc                     >  1:4968293/1‑150 (MQ=255)
                                                                                                                              ggctggcGGCCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATTGACAATGGCGGCACGGCGGCCCAGCACTGGCTGCg                    >  2:6957859/1‑150 (MQ=255)
                                                                                                                                 tggcGGCCTGCACCCCCTACAGCAACTGGTCGTAGGTGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTg                 <  2:7834032/150‑1 (MQ=255)
                                                                                                                                     ggcCTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAg             >  2:373484/1‑150 (MQ=255)
                                                                                                                                        cTGCACCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAgcgc          >  2:2553414/1‑150 (MQ=255)
                                                                                                                                            aCCCCCTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCTTCg      >  2:7569927/1‑150 (MQ=255)
                                                                                                                                             cccccTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCTTCgg     >  1:994519/1‑150 (MQ=255)
                                                                                                                                              ccccTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCTTCGGc    >  1:5940731/1‑150 (MQ=255)
                                                                                                                                              ccccTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCTTCGGc    >  1:6440851/1‑150 (MQ=255)
                                                                                                                                              ccccTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCTTCGGc    <  1:4242424/150‑1 (MQ=255)
                                                                                                                                              ccccTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCTTCGGc    <  1:3141673/150‑1 (MQ=255)
                                                                                                                                              ccccTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCTTCGGc    >  1:2521889/1‑150 (MQ=255)
                                                                                                                                               cccTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCTTCGGCa   >  1:4818322/1‑150 (MQ=255)
                                                                                                                                                ccTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCTTCGGCAt  >  1:5322604/1‑150 (MQ=255)
                                                                                                                                                ccTACAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCTTCGGCAt  >  1:3822178/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
TCGGGCACAGCACTACGCATTGGCAGTGGTAACGCTCGAGCTGCTGATGATAATGGCCCAGTTGCTCGGGCGACAGGTTACCCAGGTTGGCACCGAGCACCGCGAACTCGAAGGGCAGGCCGGCCTGGCTGGCGGCCTGCACCCCCTGCAGCAACTGGTCGTAGGAGGCGAACAGGCTCACGCTGAGGCCGCAGTCTTCCAGCTGGTGCTCCAGTGCCTGGCGCGCCAGTTCGTGGCCATCGACAATGGCGGCACGGCGGCCCAGCAATGGCTGCGGTGGCAGCGCTTCGGCAT  >  NC_002947/1842993‑1843286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑