Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_002947 1,852,005 G→A R350H (CGT→CAT)  relA → ATP:GTP 3'‑pyrophosphotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,852,0050GA99.2% 1335.9 / ‑2.6 372R350H (CGT→CAT) relAATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/3);  new base A (196/173);  total (196/176)
Fisher's exact test for biased strand distribution p-value = 1.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 372 total aligned reads displayed.

ACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCG  >  NC_002947/1851858‑1852151
                                                                                                                                                   |                                                                                                                                                  
  acCGCGCAGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATAGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATAcc                                                                                                                                                >  1:9112550/1‑150 (MQ=255)
   ccGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATAccc                                                                                                                                               >  2:4544850/1‑150 (MQ=255)
    cgcgCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCa                                                                                                                                              <  2:6387024/150‑1 (MQ=255)
      cgcTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACg                                                                                                                                            <  1:7059117/150‑1 (MQ=255)
         tGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCa                                                                                                                                         >  1:6867126/1‑150 (MQ=255)
         tGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCa                                                                                                                                         >  2:838144/1‑150 (MQ=255)
          gggCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCAt                                                                                                                                        <  1:2130475/150‑1 (MQ=255)
           ggCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATg                                                                                                                                       <  2:8500142/150‑1 (MQ=255)
           ggCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATg                                                                                                                                       >  1:4346310/1‑150 (MQ=255)
           ggCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTACTGGAGGTGCAGATCCATACCCACGGCATg                                                                                                                                       >  1:8899153/1‑150 (MQ=255)
              aTCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCAc                                                                                                                                    >  2:7052467/1‑150 (MQ=255)
                 gTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGaa                                                                                                                                 <  1:586390/150‑1 (MQ=255)
                 gTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGaa                                                                                                                                 >  1:2951991/1‑150 (MQ=255)
                   gCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAaga                                                                                                                               <  2:2831110/150‑1 (MQ=255)
                   gCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAaga                                                                                                                               >  1:744785/1‑150 (MQ=255)
                    cacaCCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAagag                                                                                                                              >  1:5942482/1‑150 (MQ=255)
                     acacCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAgg                                                                                                                             >  2:7677013/1‑150 (MQ=255)
                     acacCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAgg                                                                                                                             <  2:9046238/150‑1 (MQ=255)
                     acacCTTGTGACGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAgg                                                                                                                             >  2:6068164/1‑150 (MQ=255)
                       acCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCTTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGcc                                                                                                                           <  2:1239681/150‑1 (MQ=255)
                       acCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGcc                                                                                                                           <  1:4135843/150‑1 (MQ=255)
                       acCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGcc                                                                                                                           >  1:6393748/1‑150 (MQ=255)
                        ccTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCg                                                                                                                          >  1:4513515/1‑150 (MQ=255)
                         cTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGa                                                                                                                         >  2:194811/1‑150 (MQ=255)
                          ttGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGaa                                                                                                                        >  2:4281551/1‑150 (MQ=255)
                             tgGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACtt                                                                                                                     >  1:2786621/1‑150 (MQ=255)
                              ggcggcATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTg                                                                                                                    <  2:6724464/150‑1 (MQ=255)
                                 ggcATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCg                                                                                                                 >  2:7479563/1‑150 (MQ=255)
                                    atatTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTAt                                                                                                              >  1:9816860/1‑150 (MQ=255)
                                      atTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATgc                                                                                                            >  1:5725818/1‑150 (MQ=255)
                                      atTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATgc                                                                                                            <  1:1997755/150‑1 (MQ=255)
                                       ttCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATgcg                                                                                                           >  2:6928513/1‑150 (MQ=255)
                                       ttCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATgcg                                                                                                           >  1:3830465/1‑150 (MQ=255)
                                        tCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGTCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGTGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGTCGTATgcgc                                                                                                          <  2:8269932/150‑1 (MQ=255)
                                        tCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATgcgc                                                                                                          >  1:7808328/1‑150 (MQ=255)
                                        tCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTGGGCGTATgcgc                                                                                                          >  2:4183803/1‑150 (MQ=255)
                                         cccAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCATATGCGcc                                                                                                         <  2:9672735/150‑1 (MQ=255)
                                          ccAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGccc                                                                                                        <  1:8608560/150‑1 (MQ=255)
                                          ccAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGccc                                                                                                        >  2:5389111/1‑150 (MQ=255)
                                            aaGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCAc                                                                                                      >  1:4803032/1‑150 (MQ=255)
                                            aaGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCAc                                                                                                      >  1:824508/1‑150 (MQ=255)
                                            aaGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCAc                                                                                                      >  1:8714979/1‑150 (MQ=255)
                                            aaGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCAc                                                                                                      >  1:7184598/1‑150 (MQ=255)
                                                 gTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgcg                                                                                                 <  2:9854250/150‑1 (MQ=255)
                                                 gTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgcg                                                                                                 <  2:8115452/150‑1 (MQ=255)
                                                  ttCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATCCCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGgcgc                                                                                                <  2:6542709/150‑1 (MQ=255)
                                                        gacTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAg                                                                                          >  2:1024010/1‑150 (MQ=255)
                                                           tACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGc                                                                                       >  2:4647191/1‑150 (MQ=255)
                                                           tACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGc                                                                                       >  2:9433982/1‑150 (MQ=255)
                                                           tACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGc                                                                                       >  2:7338198/1‑150 (MQ=255)
                                                              aTCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAcc                                                                                    <  1:3543566/150‑1 (MQ=255)
                                                              aTCGCCAACCCCAAGGAGAACGGCTACCACTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCAcc                                                                                    >  2:5045281/1‑150 (MQ=255)
                                                               tCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCg                                                                                   >  2:5567831/1‑150 (MQ=255)
                                                                      ccccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCaa                                                                            <  2:1655196/150‑1 (MQ=255)
                                                                      ccccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCaa                                                                            <  2:1829983/150‑1 (MQ=255)
                                                                       cccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAg                                                                           >  1:1042225/1‑150 (MQ=255)
                                                                       cccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAg                                                                           <  1:7643332/150‑1 (MQ=255)
                                                                        ccAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGc                                                                          >  1:6068881/1‑150 (MQ=255)
                                                                          aaGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGccc                                                                        <  1:1511215/150‑1 (MQ=255)
                                                                           aGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCa                                                                       >  2:3544255/1‑150 (MQ=255)
                                                                            ggAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAg                                                                      >  1:936432/1‑150 (MQ=255)
                                                                               gaACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTc                                                                   <  2:9157173/150‑1 (MQ=255)
                                                                                 aCGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCa                                                                 >  1:5556411/1‑150 (MQ=255)
                                                                                   ggCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAAc                                                               >  2:6420640/1‑150 (MQ=255)
                                                                                    gCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAAcc                                                              >  2:9140112/1‑150 (MQ=255)
                                                                                            tCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACgaa                                                      <  2:4917874/150‑1 (MQ=255)
                                                                                               ttGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACgaagaa                                                   >  1:8152847/1‑150 (MQ=255)
                                                                                                 gCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGaaaa                                                 <  2:5184630/150‑1 (MQ=255)
                                                                                                 gCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGaaaa                                                 <  2:5775506/150‑1 (MQ=255)
                                                                                                 gCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGaaaa                                                 <  2:5694777/150‑1 (MQ=255)
                                                                                                      cTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCTTGCACGAAGAGGCCGTACTTGGCGTATGCGCCCTCTGGCGCTACAAGGGCACCGACGTCTAGCCCAGCTCCAACCACTACGAAGAAAAGAttt                                            <  1:10121128/150‑1 (MQ=255)
                                                                                                       tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTc                                           >  1:4299371/1‑150 (MQ=255)
                                                                                                       tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTc                                           <  1:333606/150‑1 (MQ=255)
                                                                                                       tGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTc                                           <  2:621251/150‑1 (MQ=255)
                                                                                                          ggTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTg                                        >  1:6685777/1‑150 (MQ=255)
                                                                                                          ggTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTg                                        <  2:2319536/150‑1 (MQ=255)
                                                                                                          ggTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGCGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTg                                        >  2:7999220/1‑150 (MQ=255)
                                                                                                           gTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTgg                                       <  1:9431434/150‑1 (MQ=255)
                                                                                                            tAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGt                                      <  1:4448816/150‑1 (MQ=255)
                                                                                                             aaTCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGtt                                     <  1:3127733/150‑1 (MQ=255)
                                                                                                                  gCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTc                                >  1:1643671/1‑150 (MQ=255)
                                                                                                                   ccccGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCa                               >  1:498014/1‑150 (MQ=255)
                                                                                                                     ccGAGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAgg                             >  1:9577641/1‑150 (MQ=255)
                                                                                                                        aGGGCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGc                          <  2:5980533/150‑1 (MQ=255)
                                                                                                                         gggCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCt                         <  2:2534264/150‑1 (MQ=255)
                                                                                                                          ggCAAGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGGCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTg                        <  1:6238341/150‑1 (MQ=255)
                                                                                                                             aaGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAg                     <  2:6180822/150‑1 (MQ=255)
                                                                                                                              aGGTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGt                    <  2:8769887/150‑1 (MQ=255)
                                                                                                                               ggTGCTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTg                   <  2:3895470/150‑1 (MQ=255)
                                                                                                                                   cTGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCAc               >  2:5440874/1‑150 (MQ=255)
                                                                                                                                    tGGAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGTTTTCGTGGTTGCGTCAGGTGCTGGAGTGGCACg              >  2:5645149/1‑150 (MQ=255)
                                                                                                                                     ggAGGTGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGa             >  2:7782038/1‑150 (MQ=255)
                                                                                                                                          tGCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGc        >  2:10034015/1‑150 (MQ=255)
                                                                                                                                           gCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCt       >  1:243346/1‑150 (MQ=255)
                                                                                                                                           gCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCt       <  2:3451203/150‑1 (MQ=255)
                                                                                                                                           gCAGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCt       >  2:3083359/1‑150 (MQ=255)
                                                                                                                                             aGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTgg     <  1:5257318/150‑1 (MQ=255)
                                                                                                                                             aGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTgg     >  1:5319197/1‑150 (MQ=255)
                                                                                                                                             aGATCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTgg     >  1:3708055/1‑150 (MQ=255)
                                                                                                                                                tCCATACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCg  >  2:955442/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
ACACCGCGCTGGGCATCGTGCACACCTTGTGGCGGCATATTCCCAAGGAGTTCGACGACTACATCGCCAACCCCAAGGAGAACGGCTACCGCTCGTTGCACACTGCGGTAATCGGCCCCGAGGGCAAGGTGCTGGAGGTGCAGATCCGTACCCACGGCATGCACGAAGAGGCCGAACTTGGCGTATGCGCCCACTGGCGCTACAAGGGCACCGACGTCAAGCCCAGCTCCAACCACTACGAAGAAAAGATTTCCTGGTTGCGTCAGGTGCTGGAGTGGCACGAAGAGCTGGGCG  >  NC_002947/1851858‑1852151

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑