Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,852,3050GC73.3% 71.1 / 32.1 45R450P (CGC→CCC) relAATP:GTP 3'‑pyrophosphotransferase
Reads supporting (aligned to +/- strand):  ref base G (5/7);  new base C (18/15);  total (23/22)
Fisher's exact test for biased strand distribution p-value = 5.14e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.68e-01
Rejected as consensus: Frequency below/above cutoff threshold.

GGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGG  >  NC_002947/1852160‑1852447
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ggcctggccGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTAATATGGTCACCCCCGACGGCCACGCCATGAAGGTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCccggc                                                                                                                                            <  1:794809/150‑1 (MQ=255)
           gCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCaa                                                                                                                                 <  1:636829/150‑1 (MQ=255)
                   gggTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTa                                                                                                                         >  2:944103/1‑150 (MQ=255)
                                     aCCGGGTTTATATGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACa                                                                                                       >  1:269603/1‑150 (MQ=255)
                                          gTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTg                                                                                                  >  2:598862/1‑150 (MQ=255)
                                              aTGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCATAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGAACACCAAGAGCGGCCACAACTGCCCCGGCTCGGAGATCAGCGGCCGTAACGTGGTGCTTGACTGTAGCCTGCAGa                                                                                              >  2:432002/1‑150 (MQ=255)
                                                  gtTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTgg                                                                                          >  1:117618/1‑150 (MQ=255)
                                                     cACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGa                                                                                       <  1:944103/150‑1 (MQ=255)
                                                            gACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTg                                                                                <  1:598862/150‑1 (MQ=255)
                                                                 ccACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGc                                                                                                     >  1:122688/1‑124 (MQ=255)
                                                                 ccACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGc                                                                                                     <  2:122688/124‑1 (MQ=255)
                                                                     gCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGatcatc                                                                       >  1:179836/1‑150 (MQ=255)
                                                                         tCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCa                                                                   <  2:642211/150‑1 (MQ=255)
                                                                           gACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCagc                                                                 >  1:56481/1‑150 (MQ=255)
                                                                             ccTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTg                                                                                           >  2:272504/1‑122 (MQ=255)
                                                                             ccTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTg                                                                                           <  1:272504/122‑1 (MQ=255)
                                                                             ccTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGCACACAACTGCCCCGTCTCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCagcaa                                                               >  1:685373/1‑150 (MQ=255)
                                                                              cTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGCCGAGCAGGTGGAGATCATCACCagcaag                                                              >  2:406820/1‑150 (MQ=255)
                                                                                gCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCaacaagca                                                            >  1:962846/1‑150 (MQ=255)
                                                                                 cccAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCAc                                                           <  1:556571/150‑1 (MQ=255)
                                                                                   cAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCccggc                                                                                                                                            <  1:541331/67‑1 (MQ=255)
                                                                                   cAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCccggc                                                                                                                                            >  2:541331/1‑67 (MQ=255)
                                                                                   cAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACgg                                                         <  2:1021501/150‑1 (MQ=255)
                                                                                          gccacgccaTTTGATGTCCCCTACCGCGTGCACCCCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTTGAGATCATCACCAGCAAGCACGGCAAcccc                                                  <  1:206516/150‑1 (MQ=255)
                                                                                          gccacgccaTTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAAcccc                                                  >  1:928519/1‑150 (MQ=255)
                                                                                                ccaTTGTACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGt                                            <  2:525963/150‑1 (MQ=255)
                                                                                                  aTTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGc                                                                                         <  2:582888/103‑1 (MQ=255)
                                                                                                  aTTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGc                                                                                         >  1:582888/1‑103 (MQ=255)
                                                                                                   ttGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAg                                                                                   >  1:358630/1‑108 (MQ=255)
                                                                                                   ttGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAg                                                                                   <  2:358630/108‑1 (MQ=255)
                                                                                                        cTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCagcaa                                                               <  1:847629/123‑1 (MQ=255)
                                                                                                        cTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCagcaa                                                               >  2:847629/1‑123 (MQ=255)
                                                                                                         ttCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGcc                                              >  1:173874/1‑139 (MQ=255)
                                                                                                         ttCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGcc                                              <  2:173874/139‑1 (MQ=255)
                                                                                                         ttCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTg                                   >  2:1002633/1‑150 (MQ=255)
                                                                                                              cTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTACCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGCTTGAACTc                              >  2:112052/1‑150 (MQ=255)
                                                                                                                      tGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTgg                      <  2:269603/150‑1 (MQ=255)
                                                                                                                        cacaCCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGc                    <  2:56481/150‑1 (MQ=255)
                                                                                                                        cacaCCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGc                    <  2:117618/150‑1 (MQ=255)
                                                                                                                        cacaCCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGc                    <  1:1002633/150‑1 (MQ=255)
                                                                                                                           acCGAAATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCGGAATGACAGCCTGCAGAGTGGCGAAGAGGTGGAGATCATCACCACCAGACACGGCAACCCCAGCCGTGACTGGTAGAACTCTAACCTGGGGTAc                 >  2:316176/1‑150 (MQ=255)
                                                                                                                                      ccACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGc                                                                                         <  2:379371/67‑1 (MQ=255)
                                                                                                                                      ccACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGc                                                                                         >  1:379371/1‑67 (MQ=255)
                                                                                                                                          aaCTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAACAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTcgcgg  <  2:962846/150‑1 (MQ=255)
                                                                                                                                            cTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACg                                                          >  2:279751/1‑92 (MQ=255)
                                                                                                                                            cTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACg                                                          <  1:279751/92‑1 (MQ=255)
                                                                                                                                            cTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTa                  >  2:520109/1‑132 (MQ=255)
                                                                                                                                            cTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTa                  <  1:520109/132‑1 (MQ=255)
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GGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTGAACTCCAACCTGGGCTACGTCACCACCTCGCGG  >  NC_002947/1852160‑1852447

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑