| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 1,852,305 | G→C | R450P (CGC→CCC) | relA → | ATP:GTP 3'‑pyrophosphotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 1,852,305 | 0 | G | C | 95.5% | 66.0 / ‑3.3 | 22 | R450P (CGC→CCC) | relA | ATP:GTP 3'‑pyrophosphotransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base C (11/10); total (11/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.62e-01 | |||||||||||
GTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTG > NC_002947/1852158‑1852414 | gTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCACCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCcccg < 1:422252/150‑1 (MQ=255) gTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATc < 1:18603/150‑1 (MQ=255) aCATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGc < 2:46787/150‑1 (MQ=255) cATCGAGCCTGCCCGTGTTTATGTGTTCACCCCCGAAGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGcc < 2:96590/150‑1 (MQ=255) aTCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCg > 2:482328/1‑150 (MQ=255) aaGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCGACGCCATCGACCTGCCCAAAGGCGCCAAGCCATTGGACTTCGCCTACCACGTGCACACCGAGATCGGCCACAACTGCGCCTGCGCTAAGATCAACGCGCGTATCGATCCGCTg > 2:255588/2‑150 (MQ=255) aCCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACa > 2:580471/1‑150 (MQ=255) aTGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGa > 1:279687/1‑150 (MQ=255) ttCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGc < 1:482328/150‑1 (MQ=255) aCCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAg > 2:441072/1‑150 (MQ=255) ccccGACGGCCACGCCATCAACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCCAAGATNAACGGCCGTNTCGTGCCGNTGAACTACAGCCTGCAGACTGGCGAGcn < 1:280048/150‑2 (MQ=255) cGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGt > 2:359928/1‑150 (MQ=255) cATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCAc < 1:359928/150‑1 (MQ=255) gACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCagc < 1:441072/150‑1 (MQ=255) cacgccaTTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAg < 1:580471/150‑1 (MQ=255) cacgccaTTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAg > 1:250939/1‑150 (MQ=255) ccaTTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGt < 1:76833/150‑1 (MQ=255) aTTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGa > 1:313333/1‑150 (MQ=255) ttCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTg > 1:96872/1‑150 (MQ=255) tGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAAc > 1:469907/1‑119 (MQ=255) tGCACACCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAAc < 2:469907/119‑1 (MQ=255) acCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGt > 2:364234/1‑123 (MQ=255) acCGAGATCGGCCACAACTGCCCCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGt < 1:364234/123‑1 (MQ=255) | GTGGCCTGGCCGAGCAGTTGCGGGTCGACATCGAGCCTGACCGGGTTTATGTGTTCACCCCCGACGGCCACGCCATCGACCTGCCCAAAGGCGCCACGCCATTGGACTTCGCCTACCGCGTGCACACCGAGATCGGCCACAACTGCCGCGGCGCGAAGATCAACGGCCGTATCGTGCCGCTGAACTACAGCCTGCAGACTGGCGAGCAGGTGGAGATCATCACCAGCAAGCACGGCAACCCCAGCCGTGACTGGTTG > NC_002947/1852158‑1852414 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |