| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_002947 | 5,609,896 | G→A | R224C (CGT→TGT) | PP_4929 ← | LysR family transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_002947 | 5,609,896 | 0 | G | A | 95.5% | 62.4 / NA | 22 | R224C (CGT→TGT) | PP_4929 | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base G (1/0); new base A (11/10); total (12/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACCAAGGCCGGCGGCCGCCAGTCGCTGTCCAGTTCGACCATGTGCGCCTGGTAGGTCGGGTACTGCACCACATGCCGGGGTAACCAGGCCCAGCCAAGGCCGCGCATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACGCCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTTTGTTGTGGCGTGATCAGCAACTGCCGGTGGCGGGCCAATTGTTGGCGGGTGACCCGGCCTTCGCCCGCCAGCGGGTGGCCGACCGCGCACACCGTGACCATC > NC_002947/5609754‑5610041 | accaAGGCCGGCGGCCGCCAGTCGCTGTCCAGTTCGACCATGTGCGCCTGGTAGGTCGGGTACTGCACCACATGCCGGGGTAACCAGGCCCAGCCAAGGCCGCGCATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACGCCATAcc > 2:144293/1‑150 (MQ=255) ccGGCGGCCGCCAGTCGCTGTCCAGTTCGACCATGTGCGCCTGGTAGGTCGGGTACTGCACCACATGCCGGGGTAACCAGGCCCAGCCAAGGCCGCGCATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACACCATACCTGCGGGc < 1:506799/150‑1 (MQ=255) ccgccAGTCGCTGTCCAGTTCGACCATGTGCGCCTGGTAGGTCGGGTACTGCACCACATGCCGGGGTAACCAGGCCCAGCCAAGGCCGCGCATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACACCATACCTGCg < 1:104786/140‑1 (MQ=255) ccgccAGTCGCTGTCCAGTTCGACCATGTGCGCCTGGTAGGTCGGGTACTGCACCACATGCCGGGGTAACCAGGCCCAGCCAAGGCCGCGCATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACACCATACCTGCg > 2:104786/1‑140 (MQ=255) tCGCTGTCCAGTTCGACCATGTGCGCCTGGTAGGTCGGGTACTGCACCACATGCCGGGGTAACCAGGCCCAGCCAAGGCCGCGCATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACACCATACCTGCGGGCTGAtcgccacaaag > 2:391106/1‑144 (MQ=255) ttGTCCAGTTCGACCATGTGCGCGTGGTAGGTCGGGTACTGCACCACATGCCGGGGTAACCAGGCCCAGCCAAGGCCACGCATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACACCATACCTGCGGGCTGATCGCCTCGccgccg < 1:573566/149‑1 (MQ=255) cATGTGCGCCTGGTAGGTCGGGTACTGCACCACATGCCGGGGGAACCAGGCCCAGCCAAGGCCGCGCATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACACCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTttg > 1:180636/1‑150 (MQ=255) tgCGCCTGGTAGGTCGGGTACTGCACCACATGCCGGGGTAACCAGGCCCAGCCAAGGCCGCGCATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACACCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTTtgttgt < 1:299344/150‑1 (MQ=255) ggTAGGTCGGGTACTGCACCACATGCCGGGGTAACCAGGCCCAGCCAAGGCCGCGCATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACACCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTTTGTTGTGGCGTGa > 2:545270/1‑150 (MQ=255) tGCACCACATGCCGGGGTAACCAGGCCCAGCCAAGGCCGCGCATCAGCAGTTCGGCCCTGGCGTAGAAACTGTCGGCACACCTTACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTTTGTTGTGGCGTGATCAGCAACTGCCgg > 1:399211/1‑150 (MQ=255) acaTGCCGGGGTAACCAGGCCCAGCCAAGGCCGCGCATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACACCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTTTGTTGTGGCGTGATCAGCAACTGCCGGTGGCgg > 2:326359/1‑150 (MQ=255) acaTGCCGGGGTAACCAGGCCCAGCCAAGGCCGCGCATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACACCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTTTGTTGTGGCGTGATCAGCAACTGCCGGTGGCgg < 2:270073/150‑1 (MQ=255) gggTAACCAGGCCCAGCCAAGGCCGCGCATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACACCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTTTGTTGTGGCGTGATCAGCAACTGCCGGTGGCGGGCCAAttg < 2:303396/150‑1 (MQ=255) ggTAACCAGGCCCAGCCAAGGCCGCGCATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACACCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTTTGTTGTGGCGTGATCAGCAACTGCCGGTGGCGGGCCAAttgt > 2:270580/1‑150 (MQ=255) aCCAGGCCCAGCCAAGGCCGCGCATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACACCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTTTGTTGTGGCGTGATCAGCAACTGCCGGTGGCGGGCCAATTGTTGGc < 1:326359/150‑1 (MQ=255) gcgcATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACACCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTTTGTTGTGGCGTGATCAGCAACTGCCGGTGGCGGGCCAATTGTTGGCGGGTGACCCGGCCTTcgcc < 1:545270/150‑1 (MQ=255) gcATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACACCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTTTGTTGTGGCGTGATCAGCAACTGCCGGTGGCGGGCCAATTGTTGGCGGGTGACCCGGCCTTcgcccg > 2:171297/1‑150 (MQ=255) cagTTCGGCCATGGCGTAGAAACTGTCGGCACACCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTTTGTTGTGGCGTGATCAGCAACTGCCGGTGGCGGGCCAATTGTTGGCGGGTGACCCGGCCTTCGCCCGCCAGCgg < 1:391106/150‑1 (MQ=255) ggCCATGGCGTAGAAACTGTCGGCACACCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTTTGTTGTGGCGTGATCAGCAACTGCCGGTGGCGGGCCAATTGTTGGCGGGTGACCCGGCCTTCGCCCGCCAGCGGGTGGcc < 1:171297/150‑1 (MQ=255) cATGGCGTAGAAACTGTCGGCACACCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTTTGTTGTGGCGTGATCAGCAACTGCCGGTGGCGGGCCAATTGTTGGCGGGTGACCCGGCCTTCGCCCGCCAGCGGGTGGCCGAc > 2:300652/1‑150 (MQ=255) tAGAAACTGTCGGCACACCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTTTGTTGTGGCGTGATCAGCAACTGCCGGTGGCGGGCCAATTGTTGGCGGGTGACCCGGCCTTCGCCCGCCAGCGGGTGGCCGACCGCGcac > 1:491000/1‑150 (MQ=255) gCACACCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTNACCGCTTTGTTGTGGCGTGATCAGCAACTGCCGGTGGCGGGCCAATTGTTGGCGGGTGACCCGGCCTTCGCCCGCCAGCGGGTGGCCGACCGCGCACACCGTGACCAtc > 1:602371/1‑150 (MQ=255) | ACCAAGGCCGGCGGCCGCCAGTCGCTGTCCAGTTCGACCATGTGCGCCTGGTAGGTCGGGTACTGCACCACATGCCGGGGTAACCAGGCCCAGCCAAGGCCGCGCATCAGCAGTTCGGCCATGGCGTAGAAACTGTCGGCACGCCATACCTGCGGGCTGATCGCCTCGCCGCCGGGGTAACCGCTTTGTTGTGGCGTGATCAGCAACTGCCGGTGGCGGGCCAATTGTTGGCGGGTGACCCGGCCTTCGCCCGCCAGCGGGTGGCCGACCGCGCACACCGTGACCATC > NC_002947/5609754‑5610041 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |