Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,329,648 Δ1 bp 5.9% intergenic (+109/+22) PP_2927 → / ← PP_2928 hypothetical protein/saccharopine dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,329,6480T. 5.9% 328.1 / 12.5 102intergenic (+109/+22)PP_2927/PP_2928hypothetical protein/saccharopine dehydrogenase
Reads supporting (aligned to +/- strand):  ref base T (47/48);  new base . (3/3);  total (51/51)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 103 total aligned reads displayed.

CGCAGACGCCGTTCAACCTGATTCTGGAGGCCCGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGACGTTGACCATGCGCTTGGC  >  NC_002947/3329499‑3329795
                                                                                                                                                       |                                                                                                                                                   
cGCAGACGCCGTTCAACCTGATTCTGGAGGCCCGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCAt                                                                                                                                                     <  2:1449793/150‑1 (MQ=255)
  cAGACGCCGTTCAACCTGATTCTGGAGGCCCGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCNGGGCTGNGGCGATCCTGGANCTGGAAAGCAGAAGGGGT‑CC‑TGCATgg                                                                                                                                                   <  1:2056773/150‑1 (MQ=255)
   aGACGGCGTTCTGCCTGATTCTGGAGGCCAGCAAGTAGAGCGTGATCGTGCGCCTTCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGTCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGc                                                                                                                                                  >  2:92138/1‑150 (MQ=255)
    gACGCCGTTCAACCTGATTCTGGAGGCCCGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCa                                                                                                                                                 <  2:545996‑M2/150‑2 (MQ=255)
        ccGTTCAACCTGATTCTGGAGGCCCGCAAGTAGAGCGTGATCTTGCGCAGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAACATCGTGGTGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCacccc                                                                                                                                             >  1:442185‑M2/1‑145 (MQ=255)
              aaCCTGATTCTGGAGGCCCGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGt                                                                                                                                       <  2:918681/150‑1 (MQ=255)
                ccTGATTCTGGAGGCCCGCAAGTAGAGCGTGATCTTGCACCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGttt                                                                                                                                     >  2:1392622/1‑150 (MQ=255)
                 cTGATTCTGGAGGCCCGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATgg                                                                                                                                                   <  2:2076346/135‑1 (MQ=255)
                 cTGATTCTGGAGGCCCGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATgg                                                                                                                                                   >  1:2076346/1‑135 (MQ=255)
                 cTGATTCTGGAGGCCCGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTg                                                                                                                                    >  1:1321105/1‑150 (MQ=255)
                 cTGATTCTGGAGGCCCGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAACAGAAGGGGTGCCATGCA‑GGccccttctgctt                                                                                                                                       >  1:1294923‑M2/1‑137 (MQ=255)
                 cTGATTCTGGAGGCCCGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAACAGAAGGGGTGCCATGCA‑GGccccttctgctt                                                                                                                                       <  2:1294923‑M2/149‑13 (MQ=255)
                  tGATTCTGGAGGCCCGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGt                                                                                                                                   >  2:1963601/1‑150 (MQ=255)
                         ggAGGCCCGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAg                                                                                                                            >  1:197600/1‑150 (MQ=255)
                               ccGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCa                                                                                                                      >  2:1974161/1‑150 (MQ=255)
                               ccGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCa                                                                                                                      <  1:7867/150‑1 (MQ=255)
                               ccGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCa                                                                                                                      <  1:1602941/150‑1 (MQ=255)
                                cGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAg                                                                                                                     >  2:2232801/1‑150 (MQ=255)
                                        gagCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAg                                                                                                             <  1:511797/150‑1 (MQ=255)
                                        gagCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAg                                                                                                             <  2:1936272/150‑1 (MQ=255)
                                         agCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAgg                                                                                                            >  1:157772/1‑150 (MQ=255)
                                         agCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAgg                                                                                                            >  1:345452/1‑150 (MQ=255)
                                           cGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGa                                                                                                          <  1:1495134/150‑1 (MQ=255)
                                           cGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGa                                                                                                          <  2:1636732/150‑1 (MQ=255)
                                            gTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTCCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGAc                                                                                                         <  1:1981462/150‑1 (MQ=255)
                                             tGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACg                                                                                                        >  1:1132339/1‑150 (MQ=255)
                                                tCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATc                                                                                                     >  1:677562/1‑150 (MQ=255)
                                                  ttGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGTGCCATGCA‑GGccccttctgctttccag                                                                                                                                  <  1:1135341‑M2/121‑18 (MQ=255)
                                                  ttGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGTGCCATGCA‑GGccccttctgctttccag                                                                                                                                  >  2:1135341‑M2/1‑104 (MQ=255)
                                                      gcCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACATGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCttt                                                                                               <  1:1369896/150‑1 (MQ=255)
                                                          gcatctTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAATTTGAAGACGT‑CC‑TGAACAGCAACCCGACTGGATGTGTCTTAGGCTTCAGCGTCCCAGGGCCGATCGCCTTTTTCa                                                                                           >  1:543112/4‑150 (MQ=255)
                                                          gCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCa                                                                                           <  1:1514391/150‑1 (MQ=255)
                                                           ccTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCAt                                                                                          <  2:1659014/150‑1 (MQ=255)
                                                            ctctTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGTGCCATGCA‑GGccccttctgctttccaggtccag                                                                                                                            >  2:1748091‑M2/1‑94 (MQ=255)
                                                            ctctTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGTGCCATGCA‑GGccccttctgctttccaggtccag                                                                                                                            <  1:1748091‑M2/117‑24 (MQ=255)
                                                            ctctTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTAAtt                                                                                         >  1:338461/1‑149 (MQ=255)
                                                             tctTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCt                                                                                        >  2:255373/1‑150 (MQ=255)
                                                                    gggCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGAcgc                                                                                 <  2:677562/150‑1 (MQ=255)
                                                                      gCTTGCCCGTTCCCCCAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCgg                                                                               <  2:345452/150‑1 (MQ=255)
                                                                      gCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCgg                                                                               >  2:591917/1‑150 (MQ=255)
                                                                      gCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCgg                                                                               <  1:1392622/150‑1 (MQ=255)
                                                                      gCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGGGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTGCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCgg                                                                               <  1:1757985/150‑1 (MQ=255)
                                                                       cTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCggg                                                                              >  2:683624/1‑150 (MQ=255)
                                                                        ttGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCa                                                                                                                      <  2:342058/109‑1 (MQ=255)
                                                                        ttGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCa                                                                                                                      >  1:342058/1‑109 (MQ=255)
                                                                        ttGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCTTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGt                                                                             >  1:1600551/1‑150 (MQ=255)
                                                                        ttGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGt                                                                             >  2:627031/1‑150 (MQ=255)
                                                                          gCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCg                                                                           >  1:1240652/1‑150 (MQ=255)
                                                                            ccGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTc                                                                                                                       >  2:2159849/1‑104 (MQ=255)
                                                                            ccGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTc                                                                                                                       <  1:2159849/104‑1 (MQ=255)
                                                                            ccGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTCTTTCATCTTTGACGCGGGTCGGc                                                                         >  1:1844186/1‑150 (MQ=255)
                                                                              gTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAg                                                                       >  1:1890476/1‑150 (MQ=255)
                                                                                   cacaGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCa                                                                  >  2:822735/1‑150 (MQ=255)
                                                                                   cacaGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCa                                                                  >  1:525080/1‑150 (MQ=255)
                                                                                    acaGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCAt                                                                 <  1:187695/150‑1 (MQ=255)
                                                                                        gTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACg                                                             >  2:1417519/1‑150 (MQ=255)
                                                                                         ttACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGAAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGAGTCCCAGGGACGATAGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGt                                                            >  1:398889/1‑150 (MQ=255)
                                                                                            cGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCa                                                         <  1:822735/150‑1 (MQ=255)
                                                                                             gTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAg                                                        <  2:281614/150‑1 (MQ=255)
                                                                                              tGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGCCGCGGGTCGGCAGGCCCATCACGTCCatc                                                       <  1:92138/150‑3 (MQ=255)
                                                                                               gCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTc                                                                                                                       <  2:1379874/85‑1 (MQ=255)
                                                                                               gCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTc                                                                                                                       >  1:1379874/1‑85 (MQ=255)
                                                                                                 ttGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGcc                                                    >  1:81452/1‑150 (MQ=255)
                                                                                                 ttGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGATTCGGCTTCAGCGTCCCATTGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGcc                                                    <  1:2099449/150‑1 (MQ=255)
                                                                                                   gTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCtt                                                  >  2:1701591/1‑150 (MQ=255)
                                                                                                     ttCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGa                                                <  1:255373/150‑1 (MQ=255)
                                                                                                     ttCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGa                                                <  1:1701591/150‑1 (MQ=255)
                                                                                                           gCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAAcg                                          <  2:1292731/150‑1 (MQ=255)
                                                                                                                 gCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAAcggctcgg                                    >  1:1057461/1‑150 (MQ=255)
                                                                                                                   gATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAAcgtctcggct                                  <  1:1417519/150‑1 (MQ=255)
                                                                                                                    aTCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTg                                 >  1:1410553/1‑150 (MQ=255)
                                                                                                                    aTCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTg                                 <  1:627031/150‑1 (MQ=255)
                                                                                                                         ggacctggaAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGc                            <  1:545996/150‑1 (MQ=255)
                                                                                                                           gcctggaAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTGGTCGCTTAGGGTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGGTGGCAGctc                          >  1:1762980/2‑150 (MQ=255)
                                                                                                                             ctggaAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGcc                                                    <  2:2378061/122‑1 (MQ=255)
                                                                                                                                aaaaGCAGGAGGGGT‑CC‑GGCATGGCGCCCCTGCTGTGTGTCGCTTAGGCTTCAGCGTCCCAGGGACGGGCGCCTTTTTCATCTTTGACGCGGGGGGGGAGGCGCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCGGGCAGCTCCTCGa                     <  2:197600/149‑1 (MQ=255)
                                                                                                                                 aaaGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGAc                    <  1:1974161/150‑1 (MQ=255)
                                                                                                                                  aaGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGatg                   >  2:623018/1‑148 (MQ=255)
                                                                                                                                    gccgcaagggT‑CC‑TCCATGGCCCCCCTTCTGTTTATTTCTAAGGCTTCACCGTCCCAGAGACGATCGCCTTTTTCATCTTTGACGCGGGTAAACAGAACCGTCACGTCCAGCGCCTTGGGGAACGGCTCGGCTGGCAGCTCCTCGACGtt                 <  2:338461/143‑1 (MQ=255)
                                                                                                                                    gCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGACGtt                 <  2:1321105/150‑1 (MQ=255)
                                                                                                                                    gCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGACGtt                 <  2:1410553/150‑1 (MQ=255)
                                                                                                                                      aGAAGGGGT‑CC‑TGCATGGCaccccttctgctttccaggtccaggatcgccacagccctgaaacaagcacgtaacctgtgggaacgggcaagcccgcgaagaggccggcgcaagatcacgctctacttgcgggcctccagaatcaggttga               <  1:832228‑M2/150‑132 (MQ=255)
                                                                                                                                      aGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGATCCTCGAccctga               >  2:1587110/1‑145 (MQ=255)
                                                                                                                                       gAAGGGGT‑CC‑TGCATGGCaccccttctgctttccaggtccaggatcgccacagccctgaaacaagcacgtaacctgtgggaacgggcaagcccgcgaagaggccggcgcaagatcacgctctacttgcgggcctccagaatcaggttgaa              <  1:2263481‑M2/150‑133 (MQ=255)
                                                                                                                                       gAAGGGGT‑CC‑TGCATGGCaccccttctgctttccaggtccaggatcgccacagccctgaaacaagc                                                                                                  >  1:1783745‑M2/1‑18 (MQ=255)
                                                                                                                                       gAAGGGGT‑CC‑TGCATGGCaccccttctgctttccaggtccaggatcgccacagccctgaaacaagc                                                                                                  <  2:1783745‑M2/66‑49 (MQ=255)
                                                                                                                                         aGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGACGTTGACCa            <  2:81452/150‑1 (MQ=255)
                                                                                                                                          ggggT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGACGTTGACCAt           >  2:1578351/1‑150 (MQ=255)
                                                                                                                                            ggT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGACGTTGa               >  1:850070/1‑144 (MQ=255)
                                                                                                                                            ggT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGACGTTGa               <  2:850070/144‑1 (MQ=255)
                                                                                                                                            ggT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGCCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGACGTTGACCATgc         >  2:233083/1‑150 (MQ=255)
                                                                                                                                             gT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGACGTTGACCATgcg        >  2:1082242/1‑150 (MQ=255)
                                                                                                                                              t‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGACGTTGACCATgcgc       >  2:1781666/1‑150 (MQ=255)
                                                                                                                                              t‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGACGTTGACCATgcgc       <  1:2232801/150‑1 (MQ=255)
                                                                                                                                                 c‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGACGTTGACCATGCGCtt     >  2:2155913/1‑150 (MQ=255)
                                                                                                                                                 c‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTCTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGCCGTTGACCATGCGCtt     <  2:1844186/150‑1 (MQ=255)
                                                                                                                                                   tGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGACGTTGACCATGCGCTTg    <  2:157772/150‑1 (MQ=255)
                                                                                                                                                    gCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGa                     <  2:1186459/132‑1 (MQ=255)
                                                                                                                                                    gCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGa                     >  1:1186459/1‑132 (MQ=255)
                                                                                                                                                     cATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGACGTTGACCATGCGCTTGGc  >  1:961975/1‑150 (MQ=255)
                                                                                                                                                       |                                                                                                                                                   
CGCAGACGCCGTTCAACCTGATTCTGGAGGCCCGCAAGTAGAGCGTGATCTTGCGCCGGCCTCTTCGCGGGCTTGCCCGTTCCCACAGGTTACGTGCTTGTTTCAGGGCTGTGGCGATCCTGGACCTGGAAAGCAGAAGGGGT‑CC‑TGCATGGCACCCCTTCTGTTTGTCGCTTAGGCTTCAGCGTCCCAGGGACGATCGCCTTTTTCATCTTTGACGCGGGTCGGCAGGCCCATCACGTCCAGCGCCTTGAGGAACGGCTCGGCTGGCAGCTCCTCGACGTTGACCATGCGCTTGGC  >  NC_002947/3329499‑3329795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑