BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,857,746	Δ1 bp	6.1%	coding (239/1371 nt)	PP_5134 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,857,746	0	A	.	6.1%	405.1 / 20.0	131	coding (239/1371 nt)	PP_5134	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (62/60); new base . (4/4); total (66/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.99e-01

Only 100 of 135 total aligned reads displayed.

CGGTTTTGGCGAAGTGTCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGC  >  NC_002947/5857597‑5857887

cGGTTTTGGCGAAGTGTCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGa                                                                                                                                                <  2:909597/150‑1 (MQ=255)

cGGTTTTGGCGAAGTGTCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGa                                                                                                                                                <  1:2283914/150‑1 (MQ=255)

            tttgtCTCACCGCCCCAGTTCTACTCTCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGG‑GCGCTCCAGGCgt                                                                                                                                   <  2:1339918/148‑2 (MQ=255)

            aGTGTCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTTGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGa                                                                                                                                   <  2:878434/150‑1 (MQ=255)

              tgtCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAgc                                                                                                                                 <  1:2095555/150‑1 (MQ=255)

              tgtCCCACCGCCCCAGCACTACCCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAgc                                                                                                                                 <  1:2014776/150‑1 (MQ=255)

                tCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGT‑CCAGGCgctcgc                                                                                                                               >  1:2249281/1‑145 (MQ=255)

                tCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAgcgc                                                                                                                               <  1:1017368/150‑1 (MQ=255)

                tCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCGTGAACGCCTTGAGCGC‑CCAGCCGAgcgc                                                                                                                               >  2:1598347/1‑150 (MQ=255)

                  ccACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGc                                                                                                                                     >  2:1128839/1‑142 (MQ=255)

                  ccACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGc                                                                                                                                     <  1:1128839/142‑1 (MQ=255)

                  ccACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCt                                                                                                                             >  1:835499/1‑150 (MQ=255)

                   cACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTg                                                                                                                            >  1:585272/1‑150 (MQ=255)

                   cACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTg                                                                                                                            >  2:443327/1‑150 (MQ=255)

                     ccGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGa                                                                                                                          <  1:1295348/150‑1 (MQ=255)

                     ccGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGa                                                                                                                          <  1:576668/150‑1 (MQ=255)

                      cGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGCTCGCCTgggc                                                                                                                         <  2:1959771/150‑3 (MQ=255)

                       gCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCCGGCGAGCGCCTGGACg                                                                                                                        <  1:1891423/150‑1 (MQ=255)

                       gCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACg                                                                                                                        >  2:2185205/1‑150 (MQ=255)

                            aGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGccca                                                                                                                   <  1:49646/150‑2 (MQ=255)

                            aGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCt                                                                                                                   >  1:466139/1‑150 (MQ=255)

                             gCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGcc                                                                                                                     >  2:1047293/1‑147 (MQ=255)

                             gCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGcc                                                                                                                     <  1:1047293/147‑1 (MQ=255)

                                   ctCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACgg                                                                                                                       >  2:2091257/1‑139 (MQ=255)

                                    tCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCt                                                                                                           >  1:134750/1‑150 (MQ=255)

                                      gCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGAGTACCTGGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTgg                                                                                                         >  2:572595/1‑150 (MQ=255)

                                       ccATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGa                                                                                                        >  2:1519109/1‑150 (MQ=255)

                                       ccATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGa                                                                                                        <  2:134750/150‑1 (MQ=255)

                                             ggAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTTGAGCGC‑CCGGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGttt                                                                                                  >  1:531070/1‑150 (MQ=255)

                                                   cgcccGGCTGGCGGTGGACGGTGAACCTTTGCTGTAGCTGTACGACACGCCCGTCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCa                                                                                            <  2:580390/146‑1 (MQ=255)

                                                    gCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAg                                                                                           <  1:235041/150‑1 (MQ=255)

                                                    gCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAg                                                                                           <  2:2403764/150‑1 (MQ=255)

                                                    gCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAg                                                                                           <  2:835499/150‑1 (MQ=255)

                                                      aCGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTg                                                                                                                            >  2:902522/1‑115 (MQ=255)

                                                      aCGGCTGTCGGTGGACGGTGAACCTTTGCTGAAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCCTTTTCTGCAggg                                                                                         >  2:582372/1‑150 (MQ=255)

                                                        ggCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCa                                                                                       >  1:315099/1‑150 (MQ=255)

                                                          cTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCt                                                                                                           <  2:1822997/128‑1 (MQ=255)

                                                          cTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCt                                                                                                           >  1:1822997/1‑128 (MQ=255)

                                                          cTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGc                                                                                     >  2:512241/1‑150 (MQ=255)

                                                                   gACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAgcgcgc                                                                            >  2:1814159/1‑150 (MQ=255)

                                                                   gACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAgcgcgc                                                                            <  1:1519109/150‑1 (MQ=255)

                                                                      ggTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCaa                                                                         <  2:1437221/150‑1 (MQ=255)

                                                                        tGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACg                                                                       >  2:472401/1‑150 (MQ=255)

                                                                        tGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTCGAACGCCTGGAGCGT‑CCAGGCGCTCGCCTgggcgc                                                                                                                       >  2:397078/1‑98 (MQ=255)

                                                                        tGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTCGAACGCCTGGAGCGT‑CCAGGCGCTCGCCTGGGCgc                                                                                                                       <  1:397078/102‑2 (MQ=255)

                                                                            ccTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTc                                                                   <  2:842140/150‑1 (MQ=255)

                                                                            ccTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTc                                                                   <  2:680754/150‑1 (MQ=255)

                                                                              tttGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCGTCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCTAACGCTTCGa                                                                 >  1:2297066/1‑150 (MQ=255)

                                                                              tttGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGa                                                                                                        <  2:1414007/111‑1 (MQ=255)

                                                                               ttGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAg                                                                <  2:1934425/150‑1 (MQ=255)

                                                                                   tGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGTTCGCCTGGACGGCCCTGCCCGGCTGGAGCGCGTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCagg                                                            >  1:1738012/1‑150 (MQ=255)

                                                                                     gAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCaggag                                                          <  1:2273722/150‑1 (MQ=255)

                                                                                          gcaCGACACGCCCGTCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGACCGCCTGGCGCGC‑CCAGGCGAGCGCCTGCACGGCCCTGCCCGGCTGGAGCGTTTTCGGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCaa                                                     <  1:2073286/148‑1 (MQ=255)

                                                                                          gTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCaa                                                     >  2:400133/1‑150 (MQ=255)

                                                                                           tACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAg                                                    >  2:168670/1‑150 (MQ=255)

                                                                                                  cGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgc                                             <  1:1814159/150‑1 (MQ=255)

                                                                                                  cGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgc                                             <  2:466139/150‑1 (MQ=255)

                                                                                                   gCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGa                                                                                                        >  1:142289/1‑90 (MQ=255)

                                                                                                   gCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcg                                            >  1:1008506/1‑150 (MQ=255)

                                                                                                    cccGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTAtt                                               <  1:908988/146‑1 (MQ=255)

                                                                                                      cGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCa                                         >  2:304293/1‑150 (MQ=255)

                                                                                                             gAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCaggag                                                          >  2:527412/1‑126 (MQ=255)

                                                                                                             gAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgctg                                  >  2:117076/1‑150 (MQ=255)

                                                                                                             gAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgctg                                  <  1:545205/150‑1 (MQ=255)

                                                                                                             gAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgctg                                  <  1:2399288/150‑1 (MQ=255)

                                                                                                                    ccATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTc                           >  2:357022/1‑150 (MQ=255)

                                                                                                                      aTCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGCGCCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCAt                         >  1:1835643/1‑150 (MQ=255)

                                                                                                                      aTCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCAt                         <  1:2185205/150‑1 (MQ=255)

                                                                                                                       tCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATg                        >  2:1913421/1‑150 (MQ=255)

                                                                                                                         gCCCTGCTCGACTACCTTGAACGCCTGGAGCGT‑CCAGGCGCTCGCCTgggc                                                                                                                         >  2:540072/1‑49 (MQ=21)

                                                                                                                         gCCCTGCTCGACTACCTTGAACGCCTGGAGCGT‑CCAGGCGCTCGCCTgggc                                                                                                                         <  1:540072/51‑3 (MQ=21)

                                                                                                                            cTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGc                   <  2:2282881/150‑1 (MQ=255)

                                                                                                                                tCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTAACGCCGGATGCCCAGGCATTTCGCCAACTGCTGGCCAGTCATGCCGGCCTGt               <  1:304293/150‑1 (MQ=255)

                                                                                                                                  gACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCTCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAt             <  1:109919/150‑1 (MQ=255)

                                                                                                                                  gACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAt             >  1:2191772/1‑150 (MQ=255)

                                                                                                                                     tACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAtgtg          >  2:1635644/1‑150 (MQ=255)

                                                                                                                                       ccTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcgc                                           >  1:118653/1‑115 (MQ=255)

                                                                                                                                       ccTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcgc                                           <  2:118653/115‑1 (MQ=255)

                                                                                                                                        cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCt                 <  1:2281517/140‑1 (MQ=255)

                                                                                                                                        cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCt                 >  2:2281517/1‑140 (MQ=255)

                                                                                                                                        cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc       >  2:1800207/1‑150 (MQ=255)

                                                                                                                                        cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc       <  1:168670/150‑1 (MQ=255)

                                                                                                                                        cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc       <  1:1913421/150‑1 (MQ=255)

                                                                                                                                        cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc       >  2:1619186/1‑150 (MQ=255)

                                                                                                                                        cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc       <  1:1280953/150‑1 (MQ=255)

                                                                                                                                        cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc       >  2:1644340/1‑150 (MQ=255)

                                                                                                                                         ttGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGACACGCCCCGACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCg      >  1:899378/1‑150 (MQ=255)

                                                                                                                                         ttGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgctg                                  >  2:809311/1‑122 (MQ=255)

                                                                                                                                         ttGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgctg                                  <  1:809311/122‑1 (MQ=255)

                                                                                                                                         ttGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGc                                >  2:180242/1‑124 (MQ=255)

                                                                                                                                         ttGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCg      >  2:969511/1‑150 (MQ=255)

                                                                                                                                          tGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTTCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGa         >  1:56903/1‑146 (MQ=255)

                                                                                                                                             tcGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCg                                                                  >  2:2019005/2‑86 (MQ=255)

                                                                                                                                             tcGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCg                                                                  <  1:2019005/85‑1 (MQ=255)

                                                                                                                                             tcGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAgg                                                   <  2:1097246/100‑1 (MQ=255)

                                                                                                                                             tcGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATgc  >  1:141447/2‑150 (MQ=255)

                                                                                                                                             aCGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATgc  <  1:2367570/150‑1 (MQ=255)

                                                                                                                                               gCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGc                   <  1:1635750/131‑1 (MQ=255)

                                                                                                                                                    gAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTg                                              <  2:2325583/99‑1 (MQ=255)

                                                                                                                                                    gAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTg                                              >  1:2325583/1‑99 (MQ=255)

CGGTTTTGGCGAAGTGTCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGC  >  NC_002947/5857597‑5857887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`