Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 1,707,335 Δ1 bp 7.7% intergenic (+111/+31) PP_1501 → / ← PP_1502 hypothetical protein/OmpA family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029471,707,3350T. 7.7% 283.6 / 17.1 91intergenic (+111/+31)PP_1501/PP_1502hypothetical protein/OmpA family protein
Reads supporting (aligned to +/- strand):  ref base T (38/46);  new base . (3/4);  total (41/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.01e-01

CATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAGTTGTCCGCTACCG  >  NC_002947/1707191‑1707481
                                                                                                                                                  |                                                                                                                                                  
cATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCgg                                                                                                                                               <  2:814252/150‑1 (MQ=255)
   gggCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGATCGACGCGGGGc                                                                                                                                            <  2:887075/150‑1 (MQ=255)
     gCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCtt                                                                                                                                          <  2:2123189/150‑1 (MQ=255)
       gAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGt                                                                                                                                        <  1:1926671/150‑1 (MQ=255)
       gAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGt                                                                                                                                        >  1:1632301/1‑150 (MQ=255)
       gAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGt                                                                                                                                        >  1:794097/1‑150 (MQ=255)
         ggAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTcc                                                                                                                                      >  2:2100269/1‑150 (MQ=255)
          gAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCCGAATTTGTGGGGCAGGCCAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGATCGACGCGGGGCttttctt                                                                                                                                     <  1:2341869/150‑6 (MQ=37)
          gAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCt                                                                                                                                     <  2:1781924/150‑1 (MQ=255)
           aaaCACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGATCGACGCGGGGCttttcttt                                                                                                                                    <  2:2109385/150‑7 (MQ=38)
             acacCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTc                                                                                                                                  <  1:1111422/150‑1 (MQ=255)
              cacCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGCGCTTGCCCTTCCa                                                                                                                                 >  2:1111422/1‑150 (MQ=255)
               acCGCGGCAGGTGCAGTGGGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAg                                                                                                                                >  2:1474271/1‑150 (MQ=255)
               acCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAg                                                                                                                                >  1:105257/1‑150 (MQ=255)
               acCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAg                                                                                                                                >  1:549862/1‑150 (MQ=255)
                ccGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGc                                                                                                                               >  2:268288/1‑150 (MQ=255)
                      cAGGTGCAGTGAGGTCTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTagtca                                                                                                                         <  2:1678677/150‑1 (MQ=255)
                        ggTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTagccagc                                                                                                                       <  2:533697/150‑1 (MQ=255)
                        ggTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTagccagc                                                                                                                       <  1:2100269/150‑1 (MQ=255)
                        ggTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTagccagc                                                                                                                       <  2:626895/150‑1 (MQ=255)
                         gTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCATAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTagccagcc                                                                                                                      <  1:581026/150‑1 (MQ=255)
                         gTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTc                                                                                                                                  >  1:141769/1‑138 (MQ=255)
                         gTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTc                                                                                                                                  <  2:141769/138‑1 (MQ=255)
                              gTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCgggg                                                                                                                                             >  1:36328/1‑122 (MQ=255)
                              gTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCgggg                                                                                                                                             <  2:36328/122‑1 (MQ=255)
                                 aGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCAGCAAGTGGCTTTGTCGGTCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCt                                                                                                              <  2:1872736/150‑1 (MQ=255)
                                         gcgcAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTgg                                                                                                      <  1:268288/150‑1 (MQ=255)
                                         gcgcAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTgg                                                                                                      <  1:300592/150‑1 (MQ=255)
                                         gcgcAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTgg                                                                                                      <  2:1632301/150‑1 (MQ=255)
                                         gcgcAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTgg                                                                                                      <  2:549862/150‑1 (MQ=255)
                                           gcagcaTTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCt                                                                                                    >  1:1097561/1‑150 (MQ=255)
                                               caTTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTagcca                                                                                                                         >  2:1334652/1‑125 (MQ=255)
                                               caTTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTagcca                                                                                                                         <  1:1334652/125‑1 (MQ=255)
                                                        cgcCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTGAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCgtggt                                                                                       <  2:1748961/150‑1 (MQ=255)
                                                           ccGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTagccagc                                                                                                                       <  2:742809/115‑1 (MQ=255)
                                                           ccGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTagccagc                                                                                                                       >  1:742809/1‑115 (MQ=255)
                                                             gtgagaTCGAGCGCCGCCCGCGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCAc                                                                                  <  2:2214376/148‑1 (MQ=255)
                                                                     gAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGt                                                                          >  1:1025658/1‑150 (MQ=255)
                                                                      aGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCt                                                                                                                                     >  1:1005346/1‑90 (MQ=255)
                                                                      aGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCt                                                                                                                                     <  2:1005346/90‑1 (MQ=255)
                                                                      aGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGtt                                                                         <  1:784344/150‑1 (MQ=255)
                                                                       gcgcCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGtt                                                                         >  1:1829964/1‑149 (MQ=255)
                                                                       gcgcCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGtt                                                                         <  2:1829964/149‑1 (MQ=255)
                                                                        cgccgcGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCAc                                                                                                 <  1:120181/124‑1 (MQ=255)
                                                                        cgccgcGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCAc                                                                                                 >  2:120181/1‑124 (MQ=255)
                                                                        cgccgcGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGt                                                                       <  2:1018594/150‑1 (MQ=255)
                                                                             cgcgGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTc                                                                                                                                  <  1:664468/86‑1 (MQ=255)
                                                                             cgcgGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTc                                                                                                                                  >  2:664468/1‑86 (MQ=255)
                                                                              gcgggcggCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTgg                                                                 <  1:105578/150‑1 (MQ=255)
                                                                                    cggcgCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATg                                                            <  2:1097561/150‑1 (MQ=255)
                                                                                       cgcTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCCCCGGCTGGTTGTCGTTGGAGATGGTc                                                         <  1:2133940/150‑1 (MQ=255)
                                                                                       cgcTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTc                                                         <  2:1025658/150‑1 (MQ=255)
                                                                                        gcTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCCCCGCGCGTGGTGCCATCGGCTGGTTGTGGTTGGAGAtcggaa                                                        >  2:1054045/1‑145 (MQ=255)
                                                                                            gATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTg                                                                  >  1:1291764/1‑136 (MQ=255)
                                                                                            gATCTCACAGGCGGCACAACACTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTg                                                                  <  2:1291764/136‑1 (MQ=255)
                                                                                                         gcACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTc                                       >  1:912601/1‑150 (MQ=255)
                                                                                                         gcACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTc                                       >  2:1358160/1‑150 (MQ=255)
                                                                                                          cacaACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCt                                      >  1:1171609/1‑150 (MQ=255)
                                                                                                           acaacaTTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTg                                     <  1:1315069/150‑1 (MQ=255)
                                                                                                             aacaTTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCt                                                     <  1:1007141/132‑1 (MQ=255)
                                                                                                             aacaTTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCt                                                     >  2:1007141/1‑132 (MQ=255)
                                                                                                                aTTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGtt                                                                   <  1:2169207/115‑1 (MQ=255)
                                                                                                                aTTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGtt                                                                   >  2:2169207/1‑115 (MQ=255)
                                                                                                                 ttATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTg                                                                                                       <  1:216562/78‑1 (MQ=255)
                                                                                                                 ttATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTg                                                                                                       >  2:216562/1‑78 (MQ=255)
                                                                                                                  tATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGt                              <  2:173899/150‑1 (MQ=255)
                                                                                                                  tATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCACTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGt                              >  1:968348/1‑150 (MQ=255)
                                                                                                                     cGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTccc                           <  1:1474271/150‑1 (MQ=255)
                                                                                                                       gCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGa                                             <  2:900512/130‑1 (MQ=255)
                                                                                                                       gCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGa                                             >  1:900512/1‑130 (MQ=255)
                                                                                                                         gAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGt                       >  1:848922/1‑150 (MQ=255)
                                                                                                                            cAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGACTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGc                    >  1:1095084/1‑150 (MQ=255)
                                                                                                                             aaaGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTgc                                    <  1:240512/133‑1 (MQ=255)
                                                                                                                             aaaGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTgc                                    >  2:240512/1‑133 (MQ=255)
                                                                                                                             aaaGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCt                   >  2:497605/1‑150 (MQ=255)
                                                                                                                             aaaGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCAACCGGCGGTTCTGCGCACGTCCCGAGTTGCt                   >  2:796638/1‑150 (MQ=255)
                                                                                                                                gAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGa                <  1:1358160/150‑1 (MQ=255)
                                                                                                                                 aaaaGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAg               >  1:1395132/1‑150 (MQ=255)
                                                                                                                                   aaGCCCCGCGATCGACGCGGGGCTTGTCCTTTCAGCTAGCGAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGga                                                                >  1:985465/1‑99 (MQ=39)
                                                                                                                                   aaGCCCCGCGATCGACGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGga                                                                <  2:985465/99‑1 (MQ=39)
                                                                                                                                   aaGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCAc                                                       <  1:2309066/108‑1 (MQ=255)
                                                                                                                                   aaGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCAc                                                       >  2:2309066/1‑108 (MQ=255)
                                                                                                                                     gCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATg                                                            >  2:579441/1‑101 (MQ=255)
                                                                                                                                     gCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATg                                                            <  1:579441/101‑1 (MQ=255)
                                                                                                                                      ccccGCGATCGACGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGTCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAGTTGTc          >  1:234795/1‑150 (MQ=255)
                                                                                                                                      ccccGCGATCGACGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAGTTGTc          >  2:1705803/1‑150 (MQ=255)
                                                                                                                                      ccccGCGCCGATCGCCGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAGCCTCGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTGGACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAGTTGTc          <  2:794097/150‑1 (MQ=255)
                                                                                                                                        ccGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCATACGCGCTTAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGCCGGTTCTGCGCACGTCCCGAGTTGCTCGAGTTGTCCg        >  1:422942/1‑150 (MQ=255)
                                                                                                                                         cgcgTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGTGCACGcccc                           <  1:1175468/130‑1 (MQ=255)
                                                                                                                                         cgcgTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGTGCACGcccc                           >  2:1175468/1‑130 (MQ=255)
                                                                                                                                               cGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAGTTGTCCGCTACCg  <  2:1935809/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CATGGGCGAGGAAACACCGCGGCAGGTGCAGTGAGGTTTCGGCGCAGCATTTTTGGCGCCCGCGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGGCACAACATTATCGGCGAACAAAGAAAAGCCCCGCGTCGATCGCGGGGCTTGTCCTTTCAGCTAGCCAGCCTTAGCGCTGAACCTGGCTCACCGAGCGTGGTGCCACCGGCTGGTTGTCGTTGGAGATGGTCACCTCCACCCGACGGTTCTGCGCACGTCCCGAGTTGCTCGAGTTGTCCGCTACCG  >  NC_002947/1707191‑1707481

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: