Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,385,840:1 +A 5.7% coding (81/576 nt) PP_2093 ← two‑component system response regulator NasT

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,385,8401.A 5.7% 429.0 / 17.2 106coding (81/576 nt)PP_2093two‑component system response regulator NasT
Reads supporting (aligned to +/- strand):  ref base . (55/45);  new base A (3/3);  total (58/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

Only 100 of 110 total aligned reads displayed.

GATGGGCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCG  >  NC_002947/2385693‑2385988
                                                                                                                                                    |                                                                                                                                                    
gATGGGCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAtc‑tc                                                                                                                                                    <  1:2181283/150‑1 (MQ=255)
gATGGGCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGAtc‑tc                                                                                                                                                    >  1:1257226/1‑150 (MQ=255)
   gggCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGaa                                                                                                                                                 <  1:405501/150‑1 (MQ=255)
       cgcgGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGA‑GATCATCGAAGccc                                                                                                                                             <  2:1990554/150‑2 (MQ=255)
               gTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCctcact                                                                                                                                     >  2:1515366/1‑150 (MQ=255)
                tCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCctcactc                                                                                                                                    >  2:855584/1‑150 (MQ=255)
                tCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCACCGCAGGCGGGCAGGTAGATGGTGAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCctgactc                                                                                                                                    >  2:1017247/1‑150 (MQ=255)
                    ggCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACg                                                                                                                                >  1:844226/1‑150 (MQ=255)
                       tGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAg                                                                                                                             <  1:990664/150‑1 (MQ=255)
                       tGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAg                                                                                                                             >  2:1038351/1‑150 (MQ=255)
                         aCCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGcc                                                                                                                           >  2:2387764/1‑150 (MQ=255)
                             gCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCa                                                                                                                       >  2:1780680/1‑150 (MQ=255)
                              cACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAg                                                                                                                      <  2:844226/150‑1 (MQ=255)
                              cACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGAGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCtgcg                                                                                                                      >  1:2209963/1‑147 (MQ=255)
                               aCTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAgg                                                                                                                     <  1:1368512/150‑1 (MQ=255)
                               aCTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAgg                                                                                                                     <  2:1538502/150‑1 (MQ=255)
                                   ccTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGcc                                                                                                                           >  1:1313632/1‑140 (MQ=255)
                                   ccTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGcc                                                                                                                           <  2:1313632/140‑1 (MQ=255)
                                   ccTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGAc                                                                                                                 >  1:375075/1‑150 (MQ=255)
                                    cTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGAcc                                                                                                                <  2:1415958/150‑1 (MQ=255)
                                          gaTCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCtt                                                                                                          >  2:1648911/2‑150 (MQ=255)
                                           aTCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTg                                                                                                         >  1:1089641/1‑150 (MQ=255)
                                           aTCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTg                                                                                                         >  2:1928898/1‑150 (MQ=255)
                                           aTCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTg                                                                                                         >  1:1417418/1‑150 (MQ=255)
                                            tCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGc                                                                                                        >  2:728954/1‑150 (MQ=255)
                                            tCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGc                                                                                                        <  1:1515366/150‑1 (MQ=255)
                                              acaTCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGcc                                                                                                                                              >  1:846814/1‑110 (MQ=255)
                                              acaTCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGcc                                                                                                                                              <  2:846814/110‑1 (MQ=255)
                                                aTCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTg                                                                                                    >  1:1338069/1‑150 (MQ=255)
                                                aTCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTg                                                                                                    >  1:1090448/1‑150 (MQ=255)
                                                 tCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTgg                                                                                                   <  1:2387764/150‑1 (MQ=255)
                                                 tCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTgg                                                                                                   <  2:120433/150‑1 (MQ=255)
                                                 tCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTgg                                                                                                   >  2:112908/1‑150 (MQ=255)
                                                 tCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTgg                                                                                                   <  1:855584/150‑1 (MQ=255)
                                                 tCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTgg                                                                                                   <  2:1090448/150‑1 (MQ=255)
                                                         tGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTgtcgtc                                                                                           <  2:1417418/150‑1 (MQ=255)
                                                           gCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAAc                                                                                                                                 >  1:1483174/1‑110 (MQ=255)
                                                           gCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAAc                                                                                                                                 <  2:1483174/110‑1 (MQ=255)
                                                                tCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTTTCGTCGATcagt                                                                                    <  2:2404608/150‑2 (MQ=255)
                                                                tCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATcagc                                                                                    <  2:2404800/150‑1 (MQ=255)
                                                                tCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATcagc                                                                                    >  2:315660/1‑150 (MQ=255)
                                                                 cGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATcagca                                                                                   <  1:1602758/150‑1 (MQ=255)
                                                                 cGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATcagca                                                                                   <  1:788560/150‑1 (MQ=255)
                                                                   gTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAgg                                                                                 >  2:2341164/1‑150 (MQ=255)
                                                                     aTCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGA‑GATCATCGAAg                                                                                                                                                <  1:35805/85‑1 (MQ=255)
                                                                       cGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATgc                                                                             <  2:1257226/150‑1 (MQ=255)
                                                                        gATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTggg                                                                                                  <  1:2197522/128‑1 (MQ=255)
                                                                         aTCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGATGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATgcgc                                                                           >  1:2338080/1‑150 (MQ=255)
                                                                           tggCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGa                                                                                <  1:799934/141‑1 (MQ=255)
                                                                           tggCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGa                                                                                >  2:799934/3‑143 (MQ=255)
                                                                             gCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTg                                                                                                    <  2:1754728/121‑1 (MQ=255)
                                                                             gCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTg                                                                                                    >  1:1754728/1‑121 (MQ=255)
                                                                                ccacGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTgg                                                                                                   >  1:2021360/1‑119 (MQ=255)
                                                                                ccacGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTgg                                                                                                   <  2:2021360/119‑1 (MQ=255)
                                                                                ccacGTCCGGGCGCACCCTTTCGACGCAGGCGGGCAGGTCGTTCGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGt                                                                    <  1:1052785/150‑1 (MQ=255)
                                                                                 cacGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGtt                                                                   >  2:2367559/1‑150 (MQ=255)
                                                                                 cacGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCACACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCACGGTCTCGTCGATCAGCAGGATGCGCAACATGtt                                                                   >  1:43479/1‑150 (MQ=255)
                                                                                 cacGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCAGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGtt                                                                   >  1:343385/1‑150 (MQ=255)
                                                                                  acGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTc                                                                  >  1:1167602/1‑150 (MQ=255)
                                                                                  acGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTc                                                                  <  1:112908/150‑1 (MQ=255)
                                                                                    gTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTgg                                                                                                   >  1:1092533/1‑115 (MQ=255)
                                                                                    gTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTgg                                                                                                   <  2:1092533/115‑1 (MQ=255)
                                                                                     tCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTgg                                                                                                   >  1:1337379/1‑114 (MQ=255)
                                                                                     tCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTgg                                                                                                   <  2:1337379/114‑1 (MQ=255)
                                                                                     tCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTc                                                               <  2:1529180/150‑1 (MQ=255)
                                                                                       cGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATca                                                                                      >  1:1082184/1‑125 (MQ=255)
                                                                                         ggCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTccccc                                                           <  1:728954/150‑1 (MQ=255)
                                                                                            gcACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCTGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCa                                                        >  1:1008274/1‑150 (MQ=255)
                                                                                            gcACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTgg                                                                                                   >  1:534962/1‑107 (MQ=255)
                                                                                            gcACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTgg                                                                                                   <  2:534962/107‑1 (MQ=255)
                                                                                             cACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCgg                                                                                                                                            <  2:1303135/65‑1 (MQ=255)
                                                                                             cACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCAc                                                       >  2:787986/1‑150 (MQ=255)
                                                                                              aCCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCTACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCAcg                                                      >  2:1695420/1‑150 (MQ=255)
                                                                                              aCCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCAcg                                                      >  2:2119552/1‑150 (MQ=255)
                                                                                                      gACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGaca                                              >  1:1526958/1‑150 (MQ=255)
                                                                                                      gACGCAGGCAGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑GCTAATCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGaca                                              >  2:1296951/1‑150 (MQ=255)
                                                                                                         gCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCTGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACgg                                           <  2:1008274/150‑1 (MQ=255)
                                                                                                                ggCGGGTAGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTGGGACGACCGGGCGTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCgg                                    <  2:687758/150‑1 (MQ=255)
                                                                                                                 gCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCggg                                   >  2:2052485/1‑150 (MQ=255)
                                                                                                                 gCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCggg                                   >  1:1300533/1‑150 (MQ=255)
                                                                                                                     gTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTgtcgtc                                                                                           >  2:2282077/1‑90 (MQ=255)
                                                                                                                       tgagggTAAGGTCTGGGGCGTCGCTGATC‑TCGAAGCCAGCCTCACGCAACGCAGCCCGCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGCGGCGTAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTc                             <  2:343385/146‑1 (MQ=255)
                                                                                                                       cGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCATCGCCGGTGTCATCCGTCAGGCGTCCGAGCTTGCTCTTTGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTc                             <  1:1740334/150‑1 (MQ=255)
                                                                                                                        gATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACggg                                          >  2:641146/1‑136 (MQ=255)
                                                                                                                        gATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACggg                                          <  1:641146/136‑1 (MQ=255)
                                                                                                                             tCAGGGCTGGGTCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCaa                       >  2:1610379/1‑150 (MQ=255)
                                                                                                                                ggTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGCTCAGCAGGATGCGCAACATATTCGTCCCCCTCCCGCGCCGACACGGGCATCGGGCAACTCGCCCacgag                    >  1:187180/1‑146 (MQ=255)
                                                                                                                                 gTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCCCTGGGAGTAGTCGATCGCCAATATTCGCGACAGGGGCGTCCCTCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCa                   >  2:1477119/1‑150 (MQ=255)
                                                                                                                                  tCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCACCTCGACCAAGGCAt                  <  2:1300533/150‑1 (MQ=255)
                                                                                                                                  tCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGAGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCAt                  <  2:2338080/150‑1 (MQ=255)
                                                                                                                                  aggccggCTTCGA‑GATCATCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCAt                  <  2:352758/145‑1 (MQ=255)
                                                                                                                                     ccggCTTCGA‑GATCATCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCa               >  2:1567780/3‑150 (MQ=255)
                                                                                                                                      cggCTTCGA‑GATCATCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCTAGGCATGCaa              >  1:2174158/2‑150 (MQ=255)
                                                                                                                                          ttCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTg          >  1:1319771/1‑150 (MQ=255)
                                                                                                                                             gatgatC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTc                                                                  >  1:1692906/1‑91 (MQ=255)
                                                                                                                                             gatgatC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTc                                                                  <  2:1692906/91‑1 (MQ=255)
                                                                                                                                              atgatC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAAc      >  2:1456154/1‑150 (MQ=255)
                                                                                                                                              atgatC‑TCGAAGCCGGCCTCACGCAAGGAAGCGCTCAGGCGACCGAGGTTGCTATGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCGCCCTCAAGCGCCGAAACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAAc      >  2:980525/1‑150 (MQ=255)
                                                                                                                                                  tc‑tcGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCg  <  1:2250354/150‑1 (MQ=255)
                                                                                                                                                  tc‑tcGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCg  <  1:992734/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GATGGGCCGCGGCTGGTCACGGCTGACCAGCACTACCTGTTCCATCACATCGCGGTCTGGCGAGTCGGTATCGATCAGCACCACGTCCGGGCGCACCGTTTCGACGCAGGCGGGCAGGTCGATGGTCAGGTCTGGGGCTTCGATGATC‑TCGAAGCCGGCCTCACTCAACGCAGCCCTCAGGCGACCGAGCTTGCTCTGGGTGTCGTCGATCAGCAGGATGCGCAACATGTTCGTCCCCCTCACGCGCCGACACGGGCATCGGGCAACTCGCCCAAGGCATGCAAGCTGAAACTGCG  >  NC_002947/2385693‑2385988

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑