Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 5,430,445:1 +A 7.1% intergenic (‑79/+82) PP_4769 ← / ← PP_4770 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029475,430,4451.A 7.1% 506.2 / 31.0 126intergenic (‑79/+82)PP_4769/PP_4770hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (58/59);  new base A (6/3);  total (64/62)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.84e-01

Only 100 of 139 total aligned reads displayed.

TGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTAT  >  NC_002947/5430296‑5430593
                                                                                                                                                      |                                                                                                                                                     
tGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAt                                                                                                                                                        <  1:1516297/150‑1 (MQ=255)
   tgacaacggtggctggggcagtgaccagggcggatacgccgacggtggttttggcagcgatgacggtgggttcttctcggacgacgattcttacgtctgacaggtctggcatactgctggcatttccgggggcgctttgcgcccctT‑cgc                                                                                                                                                    <  2:1360437‑M1/4‑1 (MQ=255)
   cAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgc                                                                                                                                                    <  1:1085003/150‑1 (MQ=255)
   cAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgc                                                                                                                                                    <  1:2409385/150‑1 (MQ=255)
    aGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgcg                                                                                                                                                   >  1:722306/1‑150 (MQ=255)
     gCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgcgc                                                                                                                                                  <  1:826131/150‑1 (MQ=255)
     gCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgcgc                                                                                                                                                  <  1:988644/150‑1 (MQ=255)
              gctggggcagtgaccagggcggatacgccgacggtggttttggcagcgatgacggtgggttcttctcggacgacgattcttacgtctgacaggtctggcatactgctggcatttccgggggcgctttgcgcccctT‑cgc                                                                                                                                                    <  1:769812‑M1/4‑1 (MQ=255)
              gctggggcagtgaccagggcggatacgccgacggtggttttggcagcgatgacggtgggttcttctcggacgacgattcttacgtctgacaggtctggcatactgctggcatttccgggggcgctttgcgcccctT‑cgc                                                                                                                                                    >  2:769812‑M1/136‑139 (MQ=255)
              cTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCg                                                                                                                                         >  1:338343/1‑150 (MQ=255)
                       ggTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCgg                                                                                                                                >  2:2033487/1‑150 (MQ=255)
                         tgtAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGc                                                                                                                              <  1:1350670/150‑1 (MQ=37)
                             gtagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGc                                                                                                                          >  2:1975583/1‑150 (MQ=255)
                             gtagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGc                                                                                                                          <  1:795496/150‑1 (MQ=255)
                             gtagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGc                                                                                                                          >  2:1222716/1‑150 (MQ=255)
                              tagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGcc                                                                                                                         >  2:1129317/1‑150 (MQ=255)
                              tagAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGcc                                                                                                                         >  2:329082/1‑150 (MQ=255)
                                    atacgccgacggtggttttggcagcgatgacggtgggttcttcgcggacgacgattcttacgtctgacaggtctggcatactgctggcatttccgggggcgctttgcgcccctT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGaa                                                                                                                   <  2:1744877‑M1/37‑1 (MQ=255)
                                     tCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAg                                                                                                                  <  2:338343/150‑1 (MQ=255)
                                        gCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGACCCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCAAAGGTAT‑CGCGCGTACACACGTGGGAGCGGGCGTGCCCGCGAAgggg                                                                                                               <  1:1558844/150‑1 (MQ=255)
                                        gCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAgggg                                                                                                               >  2:1769207/1‑150 (MQ=255)
                                        gCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAgggg                                                                                                               <  1:26362/150‑1 (MQ=255)
                                         cacaTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAgg                                                                                                                 <  2:221373/147‑1 (MQ=255)
                                         cacaTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAgg                                                                                                                 >  1:221373/1‑147 (MQ=255)
                                          acaTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGgcg                                                                                                             >  1:301706/1‑150 (MQ=255)
                                            aTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGc                                                                                                                              <  2:1167996/131‑1 (MQ=25)
                                            aTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGccc                                                                                                                        <  1:954955/137‑1 (MQ=255)
                                            aTCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGccc                                                                                                                        >  2:954955/1‑137 (MQ=255)
                                              cGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCaaa                                                                                                         >  1:1625680/1‑150 (MQ=255)
                                                gCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAgc                                                                                                       <  2:1951551/150‑1 (MQ=255)
                                                gCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAgc                                                                                                       <  1:993131/150‑1 (MQ=255)
                                                  gAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAgggg                                                                                                               >  2:645207/1‑140 (MQ=255)
                                                   aaCAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCTCGGACACGCCCGCTCCCACAGGTAG‑CGCGCGTACACCCGTGGGAGCGGGCATGCCCGCGAAGGGGCGCAAAGCG‑cc                                                                                                   >  1:169563/1‑150 (MQ=255)
                                                   aaCAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑cc                                                                                                   <  2:261211/150‑1 (MQ=255)
                                                     cAGCGCGATCTTTTTTACCTGGGAACCTCATTTTTGGCCTGGAGTGGCGGGGGGCTGCTGGGCAGACCTTCGCGGACACGCTCGTTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGCGCCCGCGAAGGGGCGCTTTGCG‑cccc                                                                                                 <  1:937177/150‑1 (MQ=255)
                                                       gcgcGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCg                                                                                               >  2:614102/1‑150 (MQ=255)
                                                         gcgATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑cgcgc                                                                                                                                                  >  2:362785/1‑98 (MQ=255)
                                                          cgATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCg                                                                                                                                 <  2:458007/114‑1 (MQ=255)
                                                          cgATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCg                                                                                                                                 >  1:458007/1‑114 (MQ=255)
                                                          cgATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGaa                                                                                            >  1:1995805/1‑150 (MQ=255)
                                                           gATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTacac                                                                                                                                            >  2:219838/1‑102 (MQ=255)
                                                                 tttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACCGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCcag                                                                                     <  1:1975583/150‑1 (MQ=255)
                                                                 tttCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCcag                                                                                     <  1:2357218/150‑1 (MQ=255)
                                                                   tCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCcagca                                                                                   >  2:1216467/1‑150 (MQ=255)
                                                                     aCCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAgc                                                                                                       >  1:67258/1‑129 (MQ=255)
                                                                        tGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATg                                                                              >  1:381130/1‑150 (MQ=255)
                                                                         gggAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg                                                                                                                                      <  2:1368090/94‑1 (MQ=255)
                                                                         gggAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTgg                                                                                                                                      >  1:1368090/1‑94 (MQ=255)
                                                                             aCCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGa                                                                         <  2:741097/150‑1 (MQ=255)
                                                                             aCCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGa                                                                         <  1:614102/150‑1 (MQ=255)
                                                                             aCCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGa                                                                         <  1:613962/150‑1 (MQ=255)
                                                                             aCCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGa                                                                         <  1:451993/150‑1 (MQ=255)
                                                                                tCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCt                                                                      >  2:1446077/1‑150 (MQ=255)
                                                                                 cATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTg                                                                     <  2:43861/150‑1 (MQ=255)
                                                                                     tttGGCCTGGAGTGCC‑GGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCGCAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCGCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGa                                                                <  2:1275609/150‑1 (MQ=255)
                                                                                          ccTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAgg                                                                                                                 >  2:1698781/1‑98 (MQ=255)
                                                                                          ccTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAgg                                                                                                                 <  1:1698781/98‑1 (MQ=255)
                                                                                          ccTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGAc                                                                        <  1:1517672/138‑1 (MQ=255)
                                                                                          ccTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGAc                                                                        >  2:1517672/1‑138 (MQ=255)
                                                                                           cTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTaa                                                           <  1:1222716/150‑1 (MQ=255)
                                                                                             ggAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGa                                                         >  2:2055997/1‑150 (MQ=255)
                                                                                              gAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCACCCAGTTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCGCCCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGa                                                         <  1:530692/150‑1 (MQ=255)
                                                                                                gTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtc                                                      <  2:584873/150‑1 (MQ=255)
                                                                                                 tGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGAc                                                                        <  1:2133130/131‑1 (MQ=255)
                                                                                                 tGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGAc                                                                        >  2:2133130/1‑131 (MQ=255)
                                                                                                  gCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcgt                                                    <  1:1769207/150‑1 (MQ=255)
                                                                                                   ccGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCaaa                                                                                                         >  1:1629492/1‑97 (MQ=255)
                                                                                                   ccGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGCGCGTGCCCGCGAAGGGGCGCaaa                                                                                                         <  2:1629492/97‑1 (MQ=255)
                                                                                                    cGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAAtcgtcg                                                  >  2:1091801/1‑150 (MQ=255)
                                                                                                        aggtctggcatactgctggcatttccgggggcgctttgcgcccctT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAgggg                                                                                                               <  1:1431751‑M1/41‑1 (MQ=255)
                                                                                                          gctgctGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCgag                                            <  2:722306/150‑1 (MQ=255)
                                                                                                          gctgctGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCga                                             <  2:1971996/149‑1 (MQ=255)
                                                                                                          gctgctGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCga                                             >  1:1971996/1‑149 (MQ=255)
                                                                                                                ccgcCGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCGGTGGGAGCGGGCGTGCCCGTGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAgaagaaa                                      <  2:1657567/149‑2 (MQ=255)
                                                                                                                   cgccccTTCGCGGGCACGCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGTCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCa                                   >  2:2247651/5‑150 (MQ=18)
                                                                                                                   cAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCca                                                                                      >  1:9464/1‑99 (MQ=255)
                                                                                                                   cAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCca                                                                                      <  2:9464/99‑1 (MQ=255)
                                                                                                                   cAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCa                                   >  2:2290433/1‑150 (MQ=255)
                                                                                                                        cTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTc                              >  1:1787235/1‑150 (MQ=255)
                                                                                                                          tCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCAt                            <  1:1058096/150‑1 (MQ=255)
                                                                                                                               gACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTg                       <  1:1129317/150‑1 (MQ=255)
                                                                                                                                    gCCCGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGTCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCaaa                  >  1:323640/1‑150 (MQ=25)
                                                                                                                                    gCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGc                                                                                        >  1:1754325/1‑80 (MQ=255)
                                                                                                                                    gCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCaaa                  >  1:343028/1‑150 (MQ=255)
                                                                                                                                      ccGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGaa                                                        <  1:2400511/110‑1 (MQ=21)
                                                                                                                                      ccGCTCCCACGGGTGTACGCGCG‑ATACCTGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGaa                                                        >  2:2400511/1‑110 (MQ=21)
                                                                                                                                      ccGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGTAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAac                >  1:1632576/1‑150 (MQ=255)
                                                                                                                                      ccGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTa                                                                                >  2:1967607/1‑86 (MQ=255)
                                                                                                                                        gCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAacca              >  1:1590638/1‑150 (MQ=255)
                                                                                                                                          tCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAaccacc            >  1:404003/1‑150 (MQ=255)
                                                                                                                                            ccACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTaa                                                           >  2:145166/1‑101 (MQ=255)
                                                                                                                                            ccACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTaa                                                           <  1:145166/101‑1 (MQ=255)
                                                                                                                                            ccACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCAt                            <  1:380978/132‑1 (MQ=255)
                                                                                                                                             cacaGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCAt                            <  2:2313251/131‑1 (MQ=255)
                                                                                                                                             cacaGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCAt                            >  1:2313251/1‑131 (MQ=255)
                                                                                                                                              acaGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCca                                                                                      <  2:57677/72‑1 (MQ=255)
                                                                                                                                              acaGGTAC‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCg        <  1:210917/150‑1 (MQ=255)
                                                                                                                                              acaGCTAT‑CGCGCGTAAACTCGTTGGAGCGCGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCTAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCg        <  1:124184/150‑1 (MQ=255)
                                                                                                                                                aGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGc      <  2:381130/150‑1 (MQ=255)
                                                                                                                                                    aT‑CGCGCGTACACCGGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTAt  <  2:169563/150‑1 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
TGGCAGCCGTGGGCCTGGCGCACGGTGTAGTAGAGGTTCTGCACATCGGCGAACAGCGCGATCTTTTTCACCTGGGAACCTCATTTTTGGCCTGGAGTGCCGGGGGGCTGCTGCGCAGACCTTCGCGGACACGCCCGCTCCCACAGGTAT‑CGCGCGTACACCCGTGGGAGCGGGCGTGCCCGCGAAGGGGCGCAAAGCG‑CCCCCGGAAATGCCAGCAGTATGCCAGACCTGTCAGACGTAAGAATCGTCGTCCGAGAAGAACCCACCGTCATCGCTGCCAAAACCACCGTCGGCGTAT  >  NC_002947/5430296‑5430593

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑