BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,441,738:1	+A	5.2%	coding (894/1653 nt)	PP_4780 ←	acyl‑CoA dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,441,738	1	.	A	5.2%	391.9 / 14.3	96	coding (894/1653 nt)	PP_4780	acyl‑CoA dehydrogenase
Reads supporting (aligned to +/- strand): ref base . (44/47); new base A (3/2); total (47/49)
Fisher's exact test for biased strand distribution p-value = 6.74e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.18e-01

Only 100 of 102 total aligned reads displayed.

TCGGCCAGCACGTTCTGCATCAATGGCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAACTGGTTGCGGTTGTCATCGGGGC  >  NC_002947/5441589‑5441886

tCGGCCAGCACGTTCTGCATCAATGGCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCAtc                                                                                                                                                       <  2:1415853/150‑1 (MQ=255)

tCGGCCAGCACGTTCTGCATCAATGGCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCAtc                                                                                                                                                       <  2:568463/150‑1 (MQ=255)

   gCCAGCACGTTCTGCATCAATGGCTGCTCGTTCAGCACCCGCCCGCTGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCg                                                                                                                                                   >  2:952661/1‑150 (MQ=255)

     cAGCACGTTCTGCATCAATGGCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCgat                                                                                                                                                 <  1:1263864/150‑1 (MQ=255)

     cAGCACGTTCTGCATCAATGGCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCgat                                                                                                                                                 >  2:1975160/1‑150 (MQ=255)

     cAGCACGTTCTGAATCAATGGCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCgat                                                                                                                                                 >  2:501998/1‑150 (MQ=255)

         aCGTTCTGCATCAATGGCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGatgatg                                                                                                                                             <  2:932466/150‑1 (MQ=255)

                 cATCAATGGCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCAc                                                                                                                                     >  2:327823/1‑150 (MQ=255)

                 cATCAATGGCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCAc                                                                                                                                     >  1:875392/1‑150 (MQ=255)

                 cATCAATGGCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCAc                                                                                                                                     >  1:2173267/1‑150 (MQ=255)

                 cATCAATGGCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCAc                                                                                                                                     <  2:2258083/150‑1 (MQ=255)

                 cATCAATGGCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCAc                                                                                                                                     >  1:1529270/1‑150 (MQ=255)

                  aTCAATGGCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCAcg                                                                                                                                    >  2:2169290/1‑150 (MQ=255)

                   tCAATGGCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCAcgc                                                                                                                                   >  1:1311114/1‑150 (MQ=255)

                   tCAATGGCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCAcgc                                                                                                                                   <  1:2169290/150‑1 (MQ=255)

                        ggCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGt                                                                                                                              <  1:88283/150‑1 (MQ=255)

                         gctgctCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCCGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTc                                                                                                                             >  2:770914/1‑150 (MQ=255)

                         gctgctCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTc                                                                                                                             >  2:711304/1‑150 (MQ=255)

                         gctgctCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTc                                                                                                                             >  2:85382/1‑150 (MQ=255)

                          ctgctCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGCCCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTcc                                                                                                                            <  1:2285093/150‑1 (MQ=255)

                          ctgctCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTcc                                                                                                                            <  1:2283355/150‑1 (MQ=255)

                          cggctCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGCACCGGGTCATGGCGACCCTC‑TCGATGATGGTCGGCACGCCGCGTcc                                                                                                                            <  2:1952499/148‑1 (MQ=255)

                           tgctCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTccc                                                                                                                           >  1:1054594/1‑150 (MQ=255)

                              tCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCctct                                                                                                                        >  1:25768/1‑150 (MQ=255)

                              tCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCctct                                                                                                                        >  1:1554074/1‑150 (MQ=255)

                              tCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCctct                                                                                                                        <  1:1481788/150‑1 (MQ=255)

                                gTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTc                                                                                                                      >  2:465438/1‑150 (MQ=255)

                                gTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTc                                                                                                                      <  1:650300/150‑1 (MQ=255)

                                  tCAGCACCCGCCCGCCGACCTTGCGATGCGCGCACTGGCGCTTCCGATCTACTGACGCCTTGCGCATCAGTGCGGCGTGGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGc                                                                                                                    <  1:541434/150‑1 (MQ=255)

                                    aGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCg                                                                                                                  >  1:320513/1‑150 (MQ=255)

                                       aCCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGatc                                                                                                               >  1:215187/1‑150 (MQ=255)

                                          cgcccgctGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGatcatc                                                                                                            <  1:952661/150‑1 (MQ=255)

                                                 cgACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCa                                                                                                     >  2:2038818/1‑150 (MQ=255)

                                                  gACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAg                                                                                                    >  2:2361316/1‑150 (MQ=255)

                                                      ttGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCCCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCa                                                                                                >  2:858099/1‑150 (MQ=255)

                                                            tGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCgg                                                                                          <  2:2173267/150‑1 (MQ=255)

                                                            tGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGGCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCgg                                                                                          >  1:869743/1‑150 (MQ=255)

                                                                 cgcaaggtcgGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTc                                                                                     <  1:1003463/145‑1 (MQ=255)

                                                                   aaTGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCa                                                                                   >  1:817500/1‑150 (MQ=255)

                                                                   aaTGGTGGGCCGCCTGCGTCAACGACTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCa                                                                                   <  2:564536/150‑1 (MQ=255)

                                                                         gggCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGatc                                                                                                               <  1:378676/116‑1 (MQ=255)

                                                                         gggCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGatc                                                                                                               >  2:378676/1‑116 (MQ=255)

                                                                             cgccTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGa                                                                         <  2:1898074/150‑1 (MQ=255)

                                                                               ccTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAgg                                                                       <  2:817500/150‑1 (MQ=255)

                                                                                     tCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTgg                                                                 >  2:1939566/1‑150 (MQ=255)

                                                                                       aaCGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAg                                                               <  1:85382/150‑1 (MQ=255)

                                                                                       aaCGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGCCGACCATCATCGA‑GATGGTCgc                                                                                                                                        <  1:661844/77‑2 (MQ=37)

                                                                                       aaCGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGCCGACCATCATCGA‑GATGGTCgc                                                                                                                                        >  2:661844/1‑76 (MQ=37)

                                                                                            cTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGtt                                                          >  2:417594/1‑150 (MQ=255)

                                                                                             tGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGa                                                                         <  1:2300328/134‑1 (MQ=255)

                                                                                             tGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGa                                                                         >  2:2300328/1‑134 (MQ=255)

                                                                                                 gcATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCa                                                     >  2:1255761/1‑150 (MQ=255)

                                                                                                 gcATCAGTGCGCTGGACCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCa                                                     <  1:1246747/150‑1 (MQ=255)

                                                                                                  cATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGCCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAg                                                    <  1:2407768/150‑1 (MQ=255)

                                                                                                      aGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTg                                                <  2:1054594/150‑1 (MQ=255)

                                                                                                      aGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTg                                                >  2:1711329/1‑150 (MQ=255)

                                                                                                      aGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGCCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTg                                                <  1:547065/150‑1 (MQ=255)

                                                                                                        tGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGatc                                                                                                               >  1:1178923/1‑85 (MQ=255)

                                                                                                        tGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGatc                                                                                                               <  2:1178923/85‑1 (MQ=255)

                                                                                                        tGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGtt                                              <  1:2038818/150‑1 (MQ=255)

                                                                                                         gcgcTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCCCGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGttc                                             <  2:25768/150‑1 (MQ=255)

                                                                                                         gcgcTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGttc                                             <  1:501998/150‑1 (MQ=255)

                                                                                                             tGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAg                                                                        >  1:91611/1‑119 (MQ=255)

                                                                                                             tGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAg                                                                        <  2:91611/119‑1 (MQ=255)

                                                                                                             tGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCa                                         >  1:1922126/1‑150 (MQ=255)

                                                                                                               gAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGttcttc                                          >  2:2044868/1‑147 (MQ=255)

                                                                                                               gAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGttcttc                                          <  1:2044868/147‑1 (MQ=255)

                                                                                                                  ccGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGgcgc                                    <  2:246189/150‑1 (MQ=255)

                                                                                                                   cGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCCCCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTGGCCCAGCTTGTTCTTCAGGCGCt                                   <  1:327823/150‑1 (MQ=255)

                                                                                                                    gACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTg                                  >  1:314799/1‑150 (MQ=255)

                                                                                                                      ccATGCAGTCGCACCGGGCCATTGCGACCTTC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGa                                <  2:2358377/150‑1 (MQ=255)

                                                                                                                         tGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGatat                             >  2:1732051/1‑150 (MQ=255)

                                                                                                                             gTCGAACCTGGTCATGGCGACCATC‑TCTACCATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCTCCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGAATATAGaa                         <  2:215187/150‑1 (MQ=255)

                                                                                                                             gTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGaa                         <  2:1529270/150‑1 (MQ=255)

                                                                                                                             gTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGaa                         >  1:1783543/1‑150 (MQ=255)

                                                                                                                              tCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAAc                        <  1:417594/150‑1 (MQ=255)

                                                                                                                              tCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAAc                        >  2:1033869/1‑150 (MQ=255)

                                                                                                                                  aCCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAACTGGt                    >  2:239538/1‑150 (MQ=255)

                                                                                                                                   ccGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTg                                                <  1:944833/121‑1 (MQ=255)

                                                                                                                                   ccGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTg                                                >  2:944833/1‑121 (MQ=255)

                                                                                                                                   ccGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGACCTGGtt                   <  2:1049539/150‑1 (MQ=255)

                                                                                                                                   ccGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAACTGGtt                   >  2:1853561/1‑150 (MQ=255)

                                                                                                                                     gggTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAACTGGTTGc                 <  1:1975160/150‑1 (MQ=255)

                                                                                                                                      ggTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTcc                                                                                    >  1:266484/1‑82 (MQ=255)

                                                                                                                                      ggTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTcc                                                                                    <  2:266484/82‑1 (MQ=255)

                                                                                                                                       gTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAACTGGTTGCgg               <  1:711304/150‑1 (MQ=255)

                                                                                                                                         cATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAACTGGTTGCGGtt             <  2:2027780/150‑1 (MQ=255)

                                                                                                                                           tgccGACCATCATCGA‑GATGGTCGCCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGa                                                                <  1:1218231/94‑1 (MQ=38)

                                                                                                                                           tgccGACCATCATCGA‑GATGGTCGCCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGa                                                                >  2:1218231/4‑97 (MQ=38)

                                                                                                                                            ggCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAACTGGTTGCGGTTGTc          >  2:129908/1‑150 (MQ=255)

                                                                                                                                             gCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAACTGGTTGCGGTTGTCa         <  2:764965/150‑1 (MQ=255)

                                                                                                                                             gCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAACTGGTTGCGGTTGTCa         >  2:1177461/1‑150 (MQ=255)

                                                                                                                                               gACCATCATCGA‑GATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAACTGGTTGCGGTTGTCATc       >  2:344013/1‑150 (MQ=255)

                                                                                                                                               gACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAACTGGTTGCGGTTGTCATc       <  2:320513/150‑1 (MQ=255)

                                                                                                                                                aCCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAACTGGTTGCGGTTGTCATCg      <  1:465438/150‑1 (MQ=255)

                                                                                                                                                  cATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAACTGGTTGCGGTTGTCATCggg    <  2:1554074/150‑1 (MQ=255)

                                                                                                                                                    tc‑tcGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGAtata                            <  1:128339/124‑1 (MQ=255)

                                                                                                                                                    tc‑tcGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGAtata                            >  2:128339/1‑124 (MQ=255)

                                                                                                                                                    tc‑tcGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAACTGGTTGCGGTTGTCATCGGGGc  >  1:2214131/1‑150 (MQ=255)

                                                                                                                                                    tc‑tcGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAACTGGTTGCGGTTGTCATCGGGGc  >  1:1632492/1‑150 (MQ=255)

TCGGCCAGCACGTTCTGCATCAATGGCTGCTCGTTCAGCACCCGCCCGCCGACCTTGCGATGCGCGCAATGGTGGGCCGCCTGCGTCAACGCCTGGCGCATCAGTGCGCTGGAGCCGACCATGCAGTCGAACCGGGTCATGGCGACCATC‑TCGATGATGGTCGGCACGCCGCGTCCCTCTTCGCCGATCATCCACGCCAGCGCACCGCGGAACTCCACCTCGCTGGAGGCGTTGGAGCTGTTGCCCAGCTTGTTCTTCAGGCGCTGGATATAGAACTGGTTGCGGTTGTCATCGGGGC  >  NC_002947/5441589‑5441886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`