BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	5,857,750:1	+T	6.1%	coding (243/1371 nt)	PP_5134 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	5,857,750	1	.	T	6.1%	527.7 / 26.6	131	coding (243/1371 nt)	PP_5134	hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (62/61); new base T (4/4); total (66/65)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.67e-01

Only 100 of 131 total aligned reads displayed.

AGTGTCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGC  >  NC_002947/5857609‑5857887

tttgtCTCACCGCCCCAGTTCTACTCTCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGG‑GCGCTCCAGGCgt                                                                                                                                   <  2:1339918/148‑2 (MQ=255)

aGTGTCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTTGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGa                                                                                                                                   <  2:878434/150‑1 (MQ=255)

  tgtCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAgc                                                                                                                                 <  1:2095555/150‑1 (MQ=255)

  tgtCCCACCGCCCCAGCACTACCCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAgc                                                                                                                                 <  1:2014776/150‑1 (MQ=255)

    tcgcACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAgcgc                                                                                                                               >  1:2403764/4‑150 (MQ=255)

    tCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGT‑CCAGGCgctcgc                                                                                                                               >  1:2249281/1‑145 (MQ=255)

    tCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAgcgc                                                                                                                               <  1:1017368/150‑1 (MQ=255)

      ccACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGc                                                                                                                                     <  1:1128839/142‑1 (MQ=255)

      ccACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGc                                                                                                                                     >  2:1128839/1‑142 (MQ=255)

      ccACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCt                                                                                                                             >  1:835499/1‑150 (MQ=255)

       cACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTg                                                                                                                            >  2:443327/1‑150 (MQ=255)

        aCCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGT‑CCAGGCGCTCGCCTgg                                                                                                                           <  2:2249281/150‑1 (MQ=255)

         ccGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGa                                                                                                                          <  1:576668/150‑1 (MQ=255)

         ccGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGa                                                                                                                          <  1:1295348/150‑1 (MQ=255)

          cGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGCTCGCCTgggc                                                                                                                         <  2:1959771/150‑3 (MQ=255)

          cGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGAc                                                                                                                         <  2:1830501/150‑1 (MQ=255)

           gCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCCGGCGAGCGCCTGGACg                                                                                                                        <  1:1891423/150‑1 (MQ=255)

           gCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACg                                                                                                                        >  2:2185205/1‑150 (MQ=255)

                aGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGccca                                                                                                                   <  1:49646/150‑2 (MQ=255)

                 gCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGcc                                                                                                                     <  1:1047293/147‑1 (MQ=255)

                 gCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGcc                                                                                                                     >  2:1047293/1‑147 (MQ=255)

                       ctCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACgg                                                                                                                       <  1:2091257/139‑1 (MQ=255)

                       ctCGCCATGTGGAAGGTGCACGGCTGTCGCTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGc                                                                                                            <  1:1839761/150‑1 (MQ=255)

                        tCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCt                                                                                                           >  1:134750/1‑150 (MQ=255)

                           ccATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGa                                                                                                        >  2:1519109/1‑150 (MQ=255)

                           ccATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGa                                                                                                        <  2:134750/150‑1 (MQ=255)

                                tgGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGtt                                                                                                   <  2:585272/150‑1 (MQ=255)

                                 ggAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTTGAGCGC‑CCGGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGttt                                                                                                  >  1:531070/1‑150 (MQ=255)

                                  gAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGtttt                                                                                                 >  2:840501/1‑150 (MQ=255)

                                       cgcccGGCTGGCGGTGGACGGTGAACCTTTGCTGTAGCTGTACGACACGCCCGTCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCa                                                                                            <  2:580390/146‑1 (MQ=255)

                                        gCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAg                                                                                           <  2:2403764/150‑1 (MQ=255)

                                        gCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAg                                                                                           <  2:835499/150‑1 (MQ=255)

                                          aCGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTg                                                                                                                            >  2:902522/1‑115 (MQ=255)

                                          aCGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTg                                                                                                                            <  1:902522/115‑1 (MQ=255)

                                          aCGGCTGTCGGTGGACGGTGAACCTTTGCTGAAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCCTTTTCTGCAggg                                                                                         >  2:582372/1‑150 (MQ=255)

                                            ggCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCa                                                                                       >  1:315099/1‑150 (MQ=255)

                                              cTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCt                                                                                                           <  2:1822997/128‑1 (MQ=255)

                                                   gggggACGGTGAACCTGTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAgc                                                                                <  1:798121/147‑1 (MQ=255)

                                                     tGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCTCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAgcgc                                                                              >  2:109919/1‑150 (MQ=255)

                                                       gACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAgcgcgc                                                                            >  2:1814159/1‑150 (MQ=255)

                                                       gACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAgcgcgc                                                                            <  1:1519109/150‑1 (MQ=255)

                                                         cGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCGGGGCAGCGAAGCGCGCCa                                                                          >  1:1573685/1‑150 (MQ=255)

                                                          ggTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCaa                                                                         <  2:1437221/150‑1 (MQ=255)

                                                            tGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACg                                                                       >  2:472401/1‑150 (MQ=255)

                                                            tGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTCGAACGCCTGGAGCGT‑CCAGGCGCTCGCCTgggcgc                                                                                                                       >  2:397078/1‑98 (MQ=255)

                                                            tGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTCGAACGCCTGGAGCGT‑CCAGGCGCTCGCCTGGGCgc                                                                                                                       <  1:397078/102‑2 (MQ=255)

                                                             gAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGc                                                                      <  2:1993561/150‑1 (MQ=255)

                                                                  tttGGTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGa                                                                 <  1:407566/150‑1 (MQ=255)

                                                                  tttGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCGTCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCTAACGCTTCGa                                                                 >  1:2297066/1‑150 (MQ=255)

                                                                  tttGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGa                                                                                                        >  1:1414007/1‑111 (MQ=255)

                                                                  tttGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGa                                                                                                        <  2:1414007/111‑1 (MQ=255)

                                                                   ttGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAg                                                                <  2:1934425/150‑1 (MQ=255)

                                                                     gCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCa                                                              <  2:315099/150‑1 (MQ=255)

                                                                     gCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTCATGCAGGCCAGCGAAGCGCGCCAACGCTTCGAGCa                                                              <  1:55179/150‑1 (MQ=255)

                                                                       tGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGTTCGCCTGGACGGCCCTGCCCGGCTGGAGCGCGTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCagg                                                            >  1:1738012/1‑150 (MQ=255)

                                                                         gAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCaggag                                                          <  1:2273722/150‑1 (MQ=255)

                                                                               tACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAg                                                    >  2:168670/1‑150 (MQ=255)

                                                                                      cGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgc                                             <  1:1814159/150‑1 (MQ=255)

                                                                                      cGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgc                                             <  2:466139/150‑1 (MQ=255)

                                                                                       gCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGa                                                                                                        >  1:142289/1‑90 (MQ=255)

                                                                                       gCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcg                                            >  1:1008506/1‑150 (MQ=255)

                                                                                        cccGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTAtt                                               >  2:908988/1‑146 (MQ=255)

                                                                                        cccGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTAtt                                               <  1:908988/146‑1 (MQ=255)

                                                                                        cccGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcgc                                           >  2:1280953/1‑150 (MQ=255)

                                                                                                 gAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCaggag                                                          >  2:527412/1‑126 (MQ=255)

                                                                                                 gAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCaggag                                                          <  1:527412/126‑1 (MQ=255)

                                                                                                 gAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgctg                                  >  2:117076/1‑150 (MQ=255)

                                                                                                 gAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgctg                                  <  1:545205/150‑1 (MQ=255)

                                                                                                 gAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgctg                                  <  1:2399288/150‑1 (MQ=255)

                                                                                                    gATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGACCGC‑CCAGGCGAGCGCCGGGACGGCCCTACCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGGATTGCGCCAACTGCTGGcc                               >  1:1988302/1‑150 (MQ=255)

                                                                                                        ccATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTc                           >  2:357022/1‑150 (MQ=255)

                                                                                                          aTCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGCGCCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCAt                         >  1:1835643/1‑150 (MQ=255)

                                                                                                          aTCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCAt                         <  1:2185205/150‑1 (MQ=255)

                                                                                                             gCCCTGCTCGACTACCTTGAACGCCTGGAGCGT‑CCAGGCGCTCGCCTgggc                                                                                                                         >  2:540072/1‑49 (MQ=21)

                                                                                                                 tGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGcc                  >  1:121259/1‑150 (MQ=255)

                                                                                                                    tCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGt               >  2:1800759/1‑150 (MQ=255)

                                                                                                                       aCTACCTTCAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGGCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGTAGGCCAAGGTATTGCGCCAACTGCTGGCCCGTCATGCCGGCCAGTTtg            >  2:481565/1‑150 (MQ=255)

                                                                                                                         tACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAtgtg          >  2:1635644/1‑150 (MQ=255)

                                                                                                                         tACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTAtgtg          >  2:1564939/1‑150 (MQ=255)

                                                                                                                           ccTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTgcgc                                           >  1:118653/1‑115 (MQ=255)

                                                                                                                            cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCt                 >  2:2281517/1‑140 (MQ=255)

                                                                                                                            cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCt                 <  1:2281517/140‑1 (MQ=255)

                                                                                                                            cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc       <  1:168670/150‑1 (MQ=255)

                                                                                                                            cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc       >  2:1800207/1‑150 (MQ=255)

                                                                                                                            cTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATc       >  2:1619186/1‑150 (MQ=255)

                                                                                                                             ttGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgctg                                  >  2:809311/1‑122 (MQ=255)

                                                                                                                             ttGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAActgctg                                  <  1:809311/122‑1 (MQ=255)

                                                                                                                             ttGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGc                                <  1:180242/124‑1 (MQ=255)

                                                                                                                              tGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTTCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGa         >  1:56903/1‑146 (MQ=255)

                                                                                                                              tGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTTCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGa         <  2:56903/146‑1 (MQ=255)

                                                                                                                               gAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGAt    <  1:2204764/150‑1 (MQ=255)

                                                                                                                                 tcGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCg                                                                  >  2:2019005/2‑86 (MQ=255)

                                                                                                                                 tcGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCg                                                                  <  1:2019005/85‑1 (MQ=255)

                                                                                                                                 tcGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAgg                                                   >  1:1097246/2‑101 (MQ=255)

                                                                                                                                 aCGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATgc  <  1:2367570/150‑1 (MQ=255)

                                                                                                                                  cGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGAt        >  1:2344377/1‑143 (MQ=255)

                                                                                                                                  cGCCTGG‑GCGCTCCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGAt        <  2:2344377/143‑1 (MQ=255)

                                                                                                                                   gCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGc                   >  2:1635750/1‑131 (MQ=255)

                                                                                                                                        gAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTg                                              >  1:2325583/1‑99 (MQ=255)

                                                                                                                                        gAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTg                                              <  2:2325583/99‑1 (MQ=255)

AGTGTCCCACCGCCCCAGCACTACTCGCCATGTGGAAGGTGCACGGCTGTCGGTGGACGGTGAACCTTTGCTGGAGCTGTACGACACGCCCGGCCTGGAAGATGCCATCGCCCTGCTCGACTACCTTGAACGCCTGGAGCGC‑CCAGGCGAGCGCCTGGACGGCCCTGCCCGGCTGGAGCGTTTTCTGCAGGGCAGCGAAGCGCGCCAACGCTTCGAGCAGGAGGCCAAGGTATTGCGCCAACTGCTGGCCAGTCATGCCGGCCTGTATGTGATCGATGC  >  NC_002947/5857609‑5857887

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`