Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,367,454 G→T 6.7% V77L (GTG→TTG)  gdhB → NAD‑specific glutamate dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,367,4540GT 6.7% 336.6 / 8.7 104V77L (GTG→TTG) gdhBNAD‑specific glutamate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (48/49);  new base T (4/3);  total (52/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 104 total aligned reads displayed.

CCTGCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTGCAAAC  >  NC_002947/2367309‑2367602
                                                                                                                                                 |                                                                                                                                                    
ccTGCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCg                                                                                                                                                  >  1:1020812/1‑150 (MQ=255)
 cTGCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCgg                                                                                                                                                 <  1:219842/150‑1 (MQ=255)
 cTGCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCgg                                                                                                                                                 <  1:1548268/150‑1 (MQ=255)
 cTGCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCgg                                                                                                                                                 >  1:1544037/1‑150 (MQ=255)
   gCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGgt                                                                                                                                               <  2:2231452/150‑1 (MQ=255)
   gCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATAATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGgt                                                                                                                                               >  2:6861/1‑150 (MQ=255)
     cacaAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGgtgt                                                                                                                                             >  1:401215/1‑150 (MQ=255)
      acaAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTa                                                                                                                                            >  2:685626/1‑150 (MQ=255)
       caAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAACACCCGCACTTGCGGgtattc                                                                                                                                           <  2:1824046/150‑5 (MQ=255)
        aaGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCgg                                                                                                                                                 <  1:49569/143‑1 (MQ=255)
        aaGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCgg                                                                                                                                                 >  2:49569/1‑143 (MQ=255)
            ggCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAAccc                                                                                                                                      <  1:685626/150‑1 (MQ=255)
             gcgcTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGTGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCt                                                                                                                                     <  1:561349/150‑1 (MQ=255)
             gcgcTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCACTTGCGGgtattcgg                                                                                                                                         <  1:2277766/146‑7 (MQ=255)
             gcgcTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCACTTGCGGgtattcgg                                                                                                                                         >  2:2277766/1‑140 (MQ=255)
             gcgcTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCt                                                                                                                                     >  2:1848650/1‑150 (MQ=255)
               gcTGTTCGCCGAGCAGTTCTTCGGCATCGCCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGa                                                                                                                                   >  1:1642560/1‑150 (MQ=255)
               gcTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGa                                                                                                                                   >  2:1418070/1‑150 (MQ=255)
                cTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGAt                                                                                                                                  <  1:1177361/150‑1 (MQ=255)
                      gCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGaa                                                                                                                            >  2:2088013/1‑150 (MQ=255)
                       ccGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAAc                                                                                                                           <  2:2150700/150‑1 (MQ=255)
                       ccGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAAc                                                                                                                           <  2:736281/150‑1 (MQ=255)
                            cAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAt                                                                                                                      <  1:1319898/150‑1 (MQ=255)
                              gTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtgg                                                                                                                    >  2:2380983/1‑150 (MQ=255)
                              gTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtgg                                                                                                                    <  1:2380983/150‑1 (MQ=255)
                                  ttcGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtggctgg                                                                                                                <  1:708490/150‑1 (MQ=255)
                                   tcGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCaa                                                                                                                       >  2:597254/1‑142 (MQ=255)
                                   tcGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCaa                                                                                                                       <  1:597254/142‑1 (MQ=255)
                                      gCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGt                                                                                                            >  1:1686942/1‑150 (MQ=255)
                                        atcatcTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTa                                                                                                                                <  2:2275939/128‑1 (MQ=255)
                                        atcatcTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTa                                                                                                                                >  1:2275939/1‑128 (MQ=255)
                                         tcatcTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGCCCCCGAATACCCGCAAGTGCGGGTGTACAACCCTCATTACGAACGCAATGGCTGGCAGTCGa                                                                                                         <  1:931827/150‑1 (MQ=255)
                                            tctctcTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGAccc                                                                                                      <  2:1020812/150‑1 (MQ=255)
                                                tctGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCCGTCGACCCATAc                                                                                                  >  1:1574167/1‑150 (MQ=255)
                                                tctGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATAc                                                                                                  >  2:672013/1‑150 (MQ=255)
                                                   ggACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGt                                                                                               <  2:401215/150‑1 (MQ=255)
                                                         aCTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtggctgg                                                                                                                >  1:1817086/1‑127 (MQ=255)
                                                         aCTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtggctgg                                                                                                                <  2:1817086/127‑1 (MQ=255)
                                                          cTCACCCAGCGCAGGCGTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGGGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGaa                                                                                        >  2:165055/1‑150 (MQ=255)
                                                           tCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCACTTGCGGgtattc                                                                                                                                           <  1:1449600/98‑5 (MQ=255)
                                                           tCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCACTTGCGGgtattc                                                                                                                                           >  2:1449600/1‑94 (MQ=255)
                                                             aCCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGtg                                                                                     >  1:1272746/1‑150 (MQ=255)
                                                               ccAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGtgct                                                                                   >  2:2292194/1‑150 (MQ=255)
                                                               ccAGAGCAGGCTTTCCGACCTGGCCTGGTTGACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGCATACCCGCAAGTGCCGGTCCACTGCGCAGATGTCGAGCGCAATCGGTGTCAGTCTACCCATACCGTGGTCGACGtgcg                                                                                   >  2:1130964/1‑149 (MQ=255)
                                                                    gcAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCAcc                                                                              <  1:1418070/150‑1 (MQ=255)
                                                                    gcAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCAcc                                                                              >  2:2267613/1‑150 (MQ=255)
                                                                     cAGGCTTTCCGACCTGGCCGGCTGGACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAAAGCAATGGCTCGCAGTCGACCCATACCCTGGTCGAAGAGATGGACCa                                                                             >  2:1822698/1‑150 (MQ=255)
                                                                        gCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGTGGGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTTCTGCAACATGa                                                                          >  1:498912/1‑150 (MQ=255)
                                                                        gCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGa                                                                          >  2:912810/1‑150 (MQ=255)
                                                                         cTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGAc                                                                         <  1:1207623/150‑1 (MQ=255)
                                                                         cTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGAc                                                                         <  2:704922/150‑1 (MQ=255)
                                                                          tttCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTg                                                                                              <  2:470257/128‑1 (MQ=255)
                                                                          tttCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTg                                                                                              >  1:470257/1‑128 (MQ=255)
                                                                              cGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGcc                                                                    <  2:1574167/150‑1 (MQ=255)
                                                                               gACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTACCg                                                                   >  2:314408/1‑150 (MQ=255)
                                                                                 ccTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAAc                                                                                                                           <  1:1918307/92‑1 (MQ=255)
                                                                                 ccTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAAc                                                                                                                           >  2:1918307/1‑92 (MQ=255)
                                                                                   tGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGCCCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTcc                                                               <  2:1642560/150‑1 (MQ=255)
                                                                                           tGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGAc                                                                         <  1:1997302/132‑1 (MQ=255)
                                                                                           tGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGAc                                                                         >  2:1997302/1‑132 (MQ=255)
                                                                                           tGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGAc                                                       >  2:898581/1‑150 (MQ=255)
                                                                                              aCCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTc                                                                                           <  2:2094507/111‑1 (MQ=255)
                                                                                              aCCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCg                                                    >  2:2233067/1‑150 (MQ=255)
                                                                                                ccTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGt                                                  <  2:1686942/150‑1 (MQ=255)
                                                                                                  tGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGTCTCGGTgc                                                <  1:165055/150‑1 (MQ=255)
                                                                                                      aGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCAc                                            >  1:438527/1‑150 (MQ=255)
                                                                                                       gCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCAcc                                           <  1:672013/150‑1 (MQ=255)
                                                                                                           ggCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAgt                                       <  1:912810/150‑2 (MQ=255)
                                                                                                              gcATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtggctgg                                                                                                                >  2:2135568/1‑74 (MQ=255)
                                                                                                              gcATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAAtggctgg                                                                                                                <  1:2135568/74‑1 (MQ=255)
                                                                                                               catcatCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCAc                                                                               <  1:1551490/106‑1 (MQ=255)
                                                                                                               catcatCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCAc                                                                               >  2:1551490/1‑106 (MQ=255)
                                                                                                                 tcatcGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAgtcgacca                                 <  1:1107898/150‑8 (MQ=255)
                                                                                                                 tcatcGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAAcc                                 <  2:1272746/150‑1 (MQ=255)
                                                                                                                    tcGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAAccgcc                              >  1:690030/1‑150 (MQ=255)
                                                                                                                      gAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGt                            <  1:2267613/150‑1 (MQ=255)
                                                                                                                      gAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGt                            <  1:1080787/150‑1 (MQ=255)
                                                                                                                           tttCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTa                       >  2:350422/1‑150 (MQ=255)
                                                                                                                            ttCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTAc                      >  1:1704382/1‑150 (MQ=255)
                                                                                                                                 ccccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCAt                 >  2:2336891/1‑150 (MQ=255)
                                                                                                                                 ccccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCAt                 <  1:898581/150‑1 (MQ=255)
                                                                                                                                 ccccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACAAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGcct                 >  2:698056/1‑148 (MQ=255)
                                                                                                                                  cccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTc                                                                                           <  2:1559474/75‑1 (MQ=255)
                                                                                                                                  cccGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTc                                                                                           >  1:1559474/1‑75 (MQ=255)
                                                                                                                                  cccGAATACCCGCAAGTGCGGGTGCACAACCCTCATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGATCTGCCGTTCCTGNTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATc                <  1:1822698/150‑1 (MQ=255)
                                                                                                                                       acaCCCGCACTTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCacac           >  2:2269311/3‑150 (MQ=255)
                                                                                                                                       acaCCCGCACTTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCacac           >  2:1248585/3‑150 (MQ=255)
                                                                                                                                           ccGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGtgct                                                                                   >  1:2178405/1‑74 (MQ=255)
                                                                                                                                           ccGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAAc                                  >  2:899176/1‑123 (MQ=255)
                                                                                                                                           ccGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGt                            >  2:2159580/1‑129 (MQ=255)
                                                                                                                                           ccGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGt                            <  1:2159580/129‑1 (MQ=255)
                                                                                                                                           ccGCAAGTGCGGGTGTACAACCCTGATTACGAACGAAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAAc                                  <  1:899176/123‑1 (MQ=255)
                                                                                                                                            cGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCAcc                                           >  1:2006574/1‑113 (MQ=255)
                                                                                                                                            cGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCAcc                                           <  2:2006574/113‑1 (MQ=255)
                                                                                                                                            cGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTGc      >  1:1794757/1‑150 (MQ=255)
                                                                                                                                             gCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTg                                                             <  2:588850/94‑1 (MQ=255)
                                                                                                                                             gCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTg                                                             >  1:588850/1‑94 (MQ=255)
                                                                                                                                              cAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTGCaa    >  2:2004699/1‑150 (MQ=255)
                                                                                                                                                aGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTGCAAac  <  1:1848650/150‑1 (MQ=255)
                                                                                                                                                aGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTGCAAac  <  1:2233067/150‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
CCTGCCACAAGTGGCGCTGTTCGCCGAGCAGTTCTTCGGCATCATCTCTCTGGACGAACTCACCCAGCGCAGGCTTTCCGACCTGGCCGGCTGCACCCTGTCAGCCTGGCGCATCATCGAGCGTTTCGACCCCGAATACCCGCAAGTGCGGGTGTACAACCCTGATTACGAACGCAATGGCTGGCAGTCGACCCATACCGTGGTCGAAGTGCTGCACCATGACCTGCCGTTCCTGGTCGACTCGGTGCGCACCGAGCTCAACCGCCGTGGCTACAGCATCCACACCCTGCAAAC  >  NC_002947/2367309‑2367602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑