Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 211,374 T→A 8.9% L5627Q (CTG→CAG)  PP_0168 → putative surface adhesion protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_002947211,3740TA 8.9% 121.8 / 4.8 45L5627Q (CTG→CAG) PP_0168putative surface adhesion protein
Reads supporting (aligned to +/- strand):  ref base T (14/27);  new base A (2/2);  total (16/29)
Fisher's exact test for biased strand distribution p-value = 6.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

CCGACGAGCCTGGCACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACG  >  NC_002947/211227‑211445
                                                                                                                                                   |                                                                       
ccGACGAGCCTGGCACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTgg                                                                       <  1:2268121/150‑1 (MQ=35)
  gacgaGCCTGGCACCCCAGGCAACCCGGGCGGCAGCAACGAAGTCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTc                                                                     <  2:115801/150‑1 (MQ=21)
  gacgaGCCTGGCACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTc                                                                     <  1:387569/150‑1 (MQ=35)
  gacgaGCCTGGCACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTc                                                                     <  2:45005/150‑1 (MQ=35)
    cgaGCCTGGCACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGt                                                                   <  2:1607852/150‑1 (MQ=35)
       gCCTGGCACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGAc                                                                <  2:1940976/150‑1 (MQ=35)
         cTGGCACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGAccc                                                              >  1:1671772/1‑150 (MQ=35)
          tGGCACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCt                                                             <  1:129913/150‑1 (MQ=35)
            gCACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGa                                                           <  2:985009/150‑1 (MQ=35)
            gCACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGa                                                           <  2:2265384/150‑1 (MQ=35)
            gCACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGa                                                           <  1:142109/150‑1 (MQ=35)
            gCACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGGCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGa                                                           <  1:1703012/150‑1 (MQ=25)
              aCCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGc                                                         <  1:368226/150‑1 (MQ=34)
               ccccAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCAGGTCGt                                                                   >  1:146782/1‑139 (MQ=37)
               ccccAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCAGGTCGt                                                                   <  2:146782/139‑1 (MQ=37)
                cccAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCTTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGcaa                                                       >  1:543359/1‑150 (MQ=25)
                    ggCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCaacaac                                                   <  1:200160/150‑1 (MQ=35)
                     gCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACg                                                  >  1:1104162/1‑150 (MQ=35)
                       aaCCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACAATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGcc                                                <  1:712732/150‑1 (MQ=25)
                          ccGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAg                                             <  2:604934/150‑1 (MQ=32)
                          ccGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAg                                             <  2:1549103/150‑1 (MQ=32)
                          ccGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAg                                             >  1:1237657/1‑150 (MQ=32)
                          ccGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAg                                             <  2:2256096/150‑1 (MQ=32)
                             ggcggcAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTc                                          <  1:357639/150‑1 (MQ=32)
                               cggcAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCAc                                        <  2:756580/150‑1 (MQ=32)
                                  cagcaACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAt                                     <  2:1013044/150‑1 (MQ=32)
                                  cagcaACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCAt                                     >  2:6385/1‑150 (MQ=32)
                                    gcaACGAAGGCGACCTGGCCAAGGTCACCATCACGGCCGACCAGCCTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCa                                   >  1:792887/1‑150 (MQ=17)
                                         gAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCAGGTCGTgggc                                                               <  1:305701/117‑4 (MQ=16)
                                         gAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCAGGTCGTgggc                                                               >  2:305701/1‑114 (MQ=16)
                                          aaGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTg                             <  2:1903256/150‑1 (MQ=32)
                                               gACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCa                                              >  2:220133/1‑128 (MQ=33)
                                               gACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCa                                              <  1:220133/128‑1 (MQ=33)
                                                 ccTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGAc                      <  1:1705050/150‑1 (MQ=32)
                                                    ggTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAg                   <  2:577173/150‑1 (MQ=32)
                                                     gTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCTACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAgc                  <  1:1193278/150‑1 (MQ=17)
                                                          ggTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGcc             >  2:2056482/1‑150 (MQ=32)
                                                            tCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGt           >  1:2133063/1‑150 (MQ=32)
                                                               ccATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTc                                                                     >  2:2388060/1‑89 (MQ=35)
                                                               ccATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTc                                                                     <  1:2388060/89‑1 (MQ=35)
                                                                cATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTacac       >  1:475170/1‑150 (MQ=32)
                                                                 aTCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGCGCAACAACCACCAGGTCGCGATCGAAACCGGCGAGACTAGGGCGCCGTACAcc      >  2:264772/1‑150 (MQ=11)
                                                                  tCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACAccc     >  1:1404230/1‑150 (MQ=32)
                                                                    aCGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCAc   >  2:697990/1‑150 (MQ=32)
                                                                     cGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGCGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACg  <  1:90713/150‑1 (MQ=17)
                                                                                                                                                   |                                                                       
CCGACGAGCCTGGCACCCCAGGCAACCCGGGCGGCAGCAACGAAGGCGACCTGGTCAAGGTCACCATCACGGCCGACCAGACTTCGTTGGCCGAGAACGTCAAACCGACGTTCACCGTACACGTCAACCAGCCGCTGGCCCACGACCTGGTCGTGACCCTGAGCAACAACGCCCAGGTCACCATCAAGGCTGGCGAGACCAGCGCGCCGTACACCCACG  >  NC_002947/211227‑211445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑