Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,390,910 T→C 5.6% R576R (CGT→CGC)  rlmKL → 23S rRNA (guanine(2445)‑N(2))/(guanine(2069)‑N(7))‑ methyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,390,9100TC 5.6% 336.5 / 6.1 106R576R (CGT→CGC) rlmKL23S rRNA (guanine(2445)‑N(2))/(guanine(2069)‑N(7))‑ methyltransferase
Reads supporting (aligned to +/- strand):  ref base T (49/51);  new base C (4/2);  total (53/53)
Fisher's exact test for biased strand distribution p-value = 6.78e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

Only 100 of 106 total aligned reads displayed.

GCGAGCGGCAAAGCGGCACGCGCCAGTACGAACGTCAGGCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTCGAAAACCTACCTGGACTGGGCACGGCGTA  >  NC_002947/2390765‑2391058
                                                                                                                                                 |                                                                                                                                                    
gcgAGCGGCAAATCGGCACGCGCCAGTACGAACGTCAGGCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATgc                                                                                                                                                  <  1:2143687/150‑1 (MQ=255)
     cGGCAAAGCGGCACGCGCCAGTACGAACGTCAGGCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATc                                                                                                                                             <  2:2164664/150‑1 (MQ=255)
        cAAAGCGGCACGCGCCAGTACGAACGTCAGGCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAg                                                                                                                                          >  2:756433/1‑150 (MQ=255)
         aaaGCGGCACGCGCCAGTACGAACGTCAGGCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAgc                                                                                                                                         >  1:2050790/1‑150 (MQ=255)
           aGCGGCACGCGCCAGTACGAACGTCAGGCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAgcgc                                                                                                                                       <  2:862954/150‑1 (MQ=255)
             cGGCACGCGCCAGTACGAACGTCAGGCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGa                                                                                                                                     <  2:61374/150‑1 (MQ=255)
              ggCACGCGCCAGTACGAACGTCAGGCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAg                                                                                                                                    <  1:462859/150‑1 (MQ=255)
               gCACGCGCCAGTACGAACGTCAGGCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAgg                                                                                                                                   <  2:371088/150‑1 (MQ=255)
               gCACGCGCCAGTACGAACGTCAGGCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAgg                                                                                                                                   <  2:1246281/150‑1 (MQ=255)
                 aCGCGCCAGTACGAACGTCAGGCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCt                                                                                                                                 >  1:689080/1‑150 (MQ=255)
                   gcgcCAGTACGAACGTCAGGCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTGCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGc                                                                                                                               >  1:263664/1‑150 (MQ=255)
                          tACGAACGTCAGGCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAg                                                                                                                        <  2:1882029/150‑1 (MQ=255)
                           aCGAACGTCAGGCTACCGAGGGCCGTTTCCAGGACGCTAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAgc                                                                                                                       <  2:193920/150‑1 (MQ=255)
                             gAACGTCAGGCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGCATACGCATcggg                                                                                                                                          <  2:890624/129‑4 (MQ=255)
                                 gTCAGGCTACCGATGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTcc                                                                                                                 >  2:1986758/1‑150 (MQ=255)
                                     ggCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCaa                                                                                                             >  2:1357477/1‑150 (MQ=255)
                                      gCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAAc                                                                                                            <  1:1336442/150‑1 (MQ=255)
                                          ccGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGt                                                                                                        <  1:1286170/150‑1 (MQ=255)
                                           cGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGtt                                                                                                       <  1:950626/150‑1 (MQ=255)
                                           cGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGtt                                                                                                       <  1:1128219/150‑1 (MQ=255)
                                            gatggCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTc                                                                                                      <  1:1986758/147‑1 (MQ=255)
                                            gAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTc                                                                                                      >  1:996720/1‑150 (MQ=255)
                                               ggCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATgcgc                                                                                                                                                >  2:771872/1‑105 (MQ=255)
                                               ggCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGc                                                                                                                               >  2:1460713/1‑122 (MQ=255)
                                               ggCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGc                                                                                                                               <  1:1460713/122‑1 (MQ=255)
                                               ggCCGTTTCCAGGAAGTGAACGAAGGTGGAGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATgcgc                                                                                                                                                <  1:771872/105‑1 (MQ=255)
                                                  cGTTTCCAGGAAGTGAACGAAGGTGGCGCCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTac                                                                                                >  2:814365/1‑150 (MQ=255)
                                                   gTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTaca                                                                                               <  2:689080/150‑1 (MQ=255)
                                                    tttCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTacac                                                                                              <  2:1008251/150‑1 (MQ=255)
                                                    tttCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTacac                                                                                              >  1:1998117/1‑150 (MQ=255)
                                                    tttCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTacac                                                                                              <  1:2067154/150‑1 (MQ=255)
                                                         aGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATCCCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCa                                                                                         >  2:130523/1‑150 (MQ=255)
                                                         aGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACAcc                                                                                             <  2:177849/146‑1 (MQ=255)
                                                         aGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACAcc                                                                                             >  1:177849/1‑146 (MQ=255)
                                                           gAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACg                                                                                       <  1:2163735/150‑1 (MQ=255)
                                                                 aaCGAAGGTGGCGTCAAGCTGCTGGTCAACCCCACAGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCAcc                                                                                 <  1:20915/150‑1 (MQ=255)
                                                                    gAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTg                                                                              <  2:2340253/150‑1 (MQ=255)
                                                                      aGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCa                                                                            >  1:1247019/1‑150 (MQ=255)
                                                                        gTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCAcg                                                                          >  1:144141/1‑150 (MQ=255)
                                                                         tGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCAcgc                                                                         <  1:1319030/150‑1 (MQ=255)
                                                                           gCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTa                                                                                                 <  1:718766/124‑1 (MQ=255)
                                                                           gCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTa                                                                                                 >  2:718766/1‑124 (MQ=255)
                                                                             gTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGcc                                                                     >  2:722990/1‑150 (MQ=255)
                                                                                 aGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAgg                                                                 <  2:1859249/150‑1 (MQ=255)
                                                                                 aGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAgg                                                                 <  1:130523/150‑1 (MQ=255)
                                                                                  gctgctGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGNTCTNCTACACCGCCACGGCCACCGTGCACGCGGCCCAggg                                                                >  2:2200061/1‑150 (MQ=255)
                                                                                     gctgGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTg                                                                                                    >  2:1641573/1‑111 (MQ=255)
                                                                                     gctgGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGAGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTg                                                                                                    <  1:1641573/111‑1 (MQ=255)
                                                                                      ctgGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGt                                                            <  2:2039092/150‑1 (MQ=255)
                                                                                       tgGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTg                                                           >  2:42253/1‑150 (MQ=255)
                                                                                            aaCCTCACCGACTCCCTGGCTCCCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTgcgcgc                                                      >  2:2020412/1‑150 (MQ=255)
                                                                                            aaCCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGGCACGGCCACCGTGCACGCGGCCAAGGGCGGTgcgcgc                                                      >  2:921184/1‑150 (MQ=255)
                                                                                            aaCCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTgcgcgc                                                      >  1:1427021/1‑150 (MQ=255)
                                                                                            aaCCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTgcgcgc                                                      >  1:221651/1‑150 (MQ=255)
                                                                                             aCCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCgca                                                     >  1:1596966/1‑150 (MQ=255)
                                                                                              ccTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACgg                                                                                      >  2:1296884/1‑116 (MQ=255)
                                                                                              ccTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACgg                                                                                      <  1:1296884/116‑1 (MQ=255)
                                                                                              ccTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCgcag                                                    >  2:895432/1‑150 (MQ=255)
                                                                                                tCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTg                                                                              >  2:2272845/1‑122 (MQ=255)
                                                                                                 cACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGcac                                                 >  1:114744/1‑150 (MQ=255)
                                                                                                   ccGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGcacca                                               <  1:756433/150‑1 (MQ=255)
                                                                                                   ccGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGcacca                                               >  2:187794/1‑150 (MQ=255)
                                                                                                    cGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAg                                                                                                                                    >  2:1368135/1‑64 (MQ=255)
                                                                                                    cGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAg                                                                                                                                    >  2:1368716/1‑64 (MQ=255)
                                                                                                    cGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAg                                                                                                                                    <  1:1368135/64‑1 (MQ=255)
                                                                                                    cGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAg                                                                                                                                    <  1:1368716/64‑1 (MQ=255)
                                                                                                        tACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGc                                                                                           >  1:1191395/1‑101 (MQ=255)
                                                                                                        tACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCAGCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGc                                                                                           <  2:1191395/101‑1 (MQ=255)
                                                                                                          ccTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAgg                                                                 <  2:835509/125‑1 (MQ=255)
                                                                                                          ccTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAgg                                                                 >  1:835509/1‑125 (MQ=255)
                                                                                                           cTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTc                                       >  2:1551807/1‑150 (MQ=255)
                                                                                                           cTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTc                                       >  1:2388171/1‑150 (MQ=255)
                                                                                                           cTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTc                                       >  2:2183008/1‑150 (MQ=255)
                                                                                                              gATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGAc                                    <  1:895432/150‑1 (MQ=255)
                                                                                                                tACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCt                                  <  1:415708/150‑1 (MQ=255)
                                                                                                                tACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCt                                  <  1:1357477/150‑1 (MQ=255)
                                                                                                                  ccGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGt                                <  2:2050790/150‑1 (MQ=255)
                                                                                                                   cGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGcacca                                               >  2:923987/1‑134 (MQ=255)
                                                                                                                   cGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGcacca                                               <  1:923987/134‑1 (MQ=255)
                                                                                                                   cGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTc                               <  2:144141/150‑1 (MQ=255)
                                                                                                                   cGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTc                               <  1:2364965/150‑1 (MQ=255)
                                                                                                                     ggCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTCGa                             >  1:1337643/1‑150 (MQ=255)
                                                                                                                        tGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTCGaaaa                          <  1:722990/150‑1 (MQ=255)
                                                                                                                        tGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTCGaaaa                          <  1:1551807/150‑1 (MQ=255)
                                                                                                                         gTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTCGAAAac                         >  1:1714/1‑150 (MQ=255)
                                                                                                                          ttCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTCGAAAacc                        >  2:308685/1‑150 (MQ=255)
                                                                                                                             cTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTCGAAAacctac                     <  1:308685/150‑1 (MQ=255)
                                                                                                                             cTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTCGAAAacctac                     <  1:2020412/150‑1 (MQ=255)
                                                                                                                                gACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTCGAAAACCTACCTg                  <  1:2183008/150‑1 (MQ=255)
                                                                                                                                   caccGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTCGAAAACCTACCTGGAc               >  1:2396870/1‑150 (MQ=255)
                                                                                                                                     ccGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTCGAAAACCTACCTGGACTg             >  1:1675300/1‑150 (MQ=255)
                                                                                                                                      cGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTg                                                                                                    <  2:342757/62‑1 (MQ=255)
                                                                                                                                      cGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTg                                                                                                    >  1:342757/1‑62 (MQ=255)
                                                                                                                                      cGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTCGAAAACCTACCTGGACTgg            >  2:2223264/1‑150 (MQ=255)
                                                                                                                                         tggATGCGCATACGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCGGCACCACCAGCGTCGACCTGTCGAAAACCTACCTGGACTGGGCa         >  1:263251/3‑150 (MQ=255)
                                                                                                                                          ggATGCGCATACGCATCGGGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGt                                                                               <  2:1143348/78‑1 (MQ=37)
                                                                                                                                          ggATGCGCATACGCATCGGGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGt                                                                               >  1:1143348/2‑79 (MQ=37)
                                                                                                                                           gATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTCGAAAACCTACCTGGACTGGGCACg       <  2:1519787/150‑1 (MQ=255)
                                                                                                                                           gATGCGCATACGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTCGAAAACCTACCTGGACTGGGCACg       >  1:1665938/1‑150 (MQ=255)
                                                                                                                                                gTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTCGAAAACCTACCTGGACTGGGCACGGCGTa  >  1:846963/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                    
GCGAGCGGCAAAGCGGCACGCGCCAGTACGAACGTCAGGCTACCGAGGGCCGTTTCCAGGAAGTGAACGAAGGTGGCGTCAAGCTGCTGGTCAACCTCACCGACTACCTGGATACCGGGCTGTTCCTCGACCACCGCCCGATGCGTATGCGCATCCAGCGCGAGGCTGCCGGCAAGCGCTTCCTCAACCTGTTCTGCTACACCGCCACGGCCACCGTGCACGCGGCCAAGGGCGGTGCGCGCAGCACCACCAGCGTCGACCTGTCGAAAACCTACCTGGACTGGGCACGGCGTA  >  NC_002947/2390765‑2391058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑