BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,410,898	T→A	8.5%	D198V (GAC→GTC)	acnA‑I ←	aconitate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,410,898	0	T	A	8.5%	316.7 / 16.7	106	D198V (GAC→GTC)	acnA‑I	aconitate hydratase
Reads supporting (aligned to +/- strand): ref base T (50/46); new base A (6/3); total (56/50)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.21e-01

Only 100 of 106 total aligned reads displayed.

GGGGATCAGCATCGATACCGGCTGGCCGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTGCGCTGCATTTC  >  NC_002947/2410750‑2411046

ggggATCAGCATCGATACCGGCTGGCCGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTc                                                                                                                                                     <  1:399670/150‑1 (MQ=255)

ggggATCAGCATCGATACCGGCTGGCCGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTc                                                                                                                                                     <  1:483726/150‑1 (MQ=255)

 gggATCAGCATCGATACCGGCTGGCCGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCg                                                                                                                                                    <  2:535203/150‑1 (MQ=255)

  ggATCAGCATCGATACCGGCTGGCCGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCgg                                                                                                                                                   <  1:1366700/150‑1 (MQ=255)

   gATCAGCATCGATACCGGCTGGCCGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCCAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGc                                                                                                                                                  <  2:841136/150‑1 (MQ=255)

        gCATCGATACCGGCTGGCCGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCAc                                                                                                                                             >  2:1966430/1‑150 (MQ=255)

          aTCGATACCGGCTGGCCGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCAc                                                                                                                                             >  2:1378780/1‑148 (MQ=255)

          aTCGATACCGGCTGGCCGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCAc                                                                                                                                             <  1:1378780/148‑1 (MQ=255)

                          cGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGc                                                                                                                           >  2:1886129/1‑150 (MQ=255)

                          cGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGc                                                                                                                           >  1:1925094/1‑150 (MQ=255)

                          cGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGc                                                                                                                           >  2:585110/1‑150 (MQ=255)

                                 ggCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCgg                                                                                                                            >  1:378191/1‑142 (MQ=255)

                                 ggCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCgg                                                                                                                            <  2:378191/142‑1 (MQ=255)

                                    cgcttccgcttcGATGCCGCCGACACCCCAGCCGAGTCCGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTc                                                                                                                 <  2:484713/150‑1 (MQ=255)

                                    cgcttccgcttcGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTc                                                                                                                 <  1:1552184/150‑1 (MQ=255)

                                        tccgcttcGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAgg                                                                                                             <  1:829612/150‑1 (MQ=255)

                                         ccgcttcGATGCCGCCGACACCGCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGt                                                                                                            <  2:1856690/150‑1 (MQ=255)

                                             ttcGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGAc                                                                                                        >  2:1166639/1‑150 (MQ=255)

                                               cGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCt                                                                                                      <  2:367267/150‑1 (MQ=255)

                                                gATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCtg                                                                                                     <  1:40274/150‑1 (MQ=255)

                                                gATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCAGGGTGGTGCGGAAGACTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCtg                                                                                                     >  2:1791354/1‑150 (MQ=255)

                                                 aTGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCTCGGGTCCAAACCGTGCGGCCCAGGTATTCCAGGTTGACCtgg                                                                                                    <  2:1077561/150‑1 (MQ=255)

                                                    ccgccgACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCtggtgg                                                                                                 <  2:1864200/150‑1 (MQ=255)

                                                     cgccgACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGc                                                                                                >  1:2032944/1‑150 (MQ=255)

                                                     cgccgACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGGGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGc                                                                                                <  1:35863/150‑1 (MQ=255)

                                                             ccccAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCg                                                                                        <  2:2032944/150‑1 (MQ=255)

                                                              cccAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCtggtgg                                                                                                 <  2:1805940/140‑1 (MQ=255)

                                                              cccAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCgg                                                                                       >  1:2064431/1‑150 (MQ=255)

                                                              cccAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCgg                                                                                       >  2:1632428/1‑150 (MQ=255)

                                                              cccAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCAGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCgg                                                                                       >  1:19948/1‑150 (MQ=255)

                                                              cccAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGGGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTGGACCTGGTGGCAAATGCCgg                                                                                       >  1:445685/1‑150 (MQ=255)

                                                                aaGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGTGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTg                                                                                     >  2:1653998/2‑150 (MQ=255)

                                                                 aGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCa                                                                                                                        >  1:1915348/1‑114 (MQ=255)

                                                                 aGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCa                                                                                                                        <  2:1915348/114‑1 (MQ=255)

                                                                     gAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCgg                                                                                >  1:1662150/1‑150 (MQ=255)

                                                                      aGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCggg                                                                               >  1:33374/1‑150 (MQ=255)

                                                                       gTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCgggg                                                                              <  2:269379/150‑1 (MQ=255)

                                                                        tACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTAGTCTAGGTTGCCCTGGTGGCAAATGCCGGTGCCCggggg                                                                             <  1:1067521/150‑1 (MQ=255)

                                                                         aCGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCggg                                                                               <  1:1217129/147‑1 (MQ=255)

                                                                         aCGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCggg                                                                               >  2:1217129/1‑147 (MQ=255)

                                                                           gCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGAc                                                                          >  1:717388/1‑150 (MQ=255)

                                                                             ccAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCg                                                                                        >  2:1169154/1‑134 (MQ=255)

                                                                             ccAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCa                                                                        >  1:821108/1‑150 (MQ=255)

                                                                             ccAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGGCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCg                                                                                        <  1:1169154/134‑1 (MQ=255)

                                                                              cAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCAc                                                                       >  1:188664/1‑150 (MQ=255)

                                                                                ggCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCTACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCAcgc                                                                     <  2:605280/150‑1 (MQ=255)

                                                                                       gATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGtt                                                              >  1:194291/1‑150 (MQ=255)

                                                                                           aTGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGAAGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCg                                                          >  2:1534711/1‑150 (MQ=255)

                                                                                            tggtggTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTCACACCGAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGCGGGACCACGCGGAAGTTGTCGa                                                         >  1:1956557/1‑150 (MQ=255)

                                                                                              gtggtgTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAg                                                       <  1:288717/150‑1 (MQ=255)

                                                                                               tggtgTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAgg                                                      <  2:1192838/150‑1 (MQ=255)

                                                                                                ggtgTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGgc                                                     <  2:2064431/150‑1 (MQ=255)

                                                                                                   gtgCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGct                                                  >  1:396812/1‑150 (MQ=255)

                                                                                                       gAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCCGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGctctct                                              <  2:445685/150‑3 (MQ=255)

                                                                                                        aGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGTTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGc                                             <  1:1632428/150‑1 (MQ=255)

                                                                                                        aGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGc                                             <  1:1886129/150‑1 (MQ=255)

                                                                                                             gtgCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCaa                                        <  1:1791354/150‑1 (MQ=255)

                                                                                                              tgCCCACCATGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCTAATGCGCTCTGGCCCCAAc                                       >  2:2113462/1‑150 (MQ=255)

                                                                                                              tgCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAAc                                       <  2:1925094/150‑1 (MQ=255)

                                                                                                               gCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACg                                      >  1:175930/1‑150 (MQ=255)

                                                                                                               gCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGAAGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACg                                      >  1:2000435/1‑150 (MQ=255)

                                                                                                                cccACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGc                                     >  1:890645/1‑150 (MQ=255)

                                                                                                                cccACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGc                                     >  2:2089752/1‑150 (MQ=255)

                                                                                                                  caccaAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAg                                   >  1:474886/1‑150 (MQ=255)

                                                                                                                     caAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGaaa                                >  1:660493/1‑150 (MQ=255)

                                                                                                                     caAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGaaa                                <  2:1662150/150‑1 (MQ=255)

                                                                                                                     caAGGTATCGGGGAAGGCATAGGTGCGGCAGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGaaa                                <  2:19948/150‑1 (MQ=255)

                                                                                                                      aaGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAg                               >  2:1859326/1‑150 (MQ=255)

                                                                                                                      aaGGTATCCGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAg                               <  2:1939511/150‑1 (MQ=255)

                                                                                                                         gTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCa                            <  1:983001/150‑1 (MQ=255)

                                                                                                                            tCGGGGAAGGCATAGGTGCGGCCGTCGGCCTGACGGGCTTACGCCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAg                         <  2:2000435/150‑1 (MQ=255)

                                                                                                                            tCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAg                         >  2:367163/1‑150 (MQ=255)

                                                                                                                              ggggAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGTACCAACGCAGAAAGGCATAgcg                       >  1:353852/1‑150 (MQ=255)

                                                                                                                                 gAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTc                    <  2:890645/150‑1 (MQ=255)

                                                                                                                                    ggCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGaa                                                                 >  2:1827113/1‑102 (MQ=255)

                                                                                                                                    ggCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGaa                                                                 <  1:1827113/102‑1 (MQ=255)

                                                                                                                                     gCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTGGcga                >  1:1611055/1‑148 (MQ=255)

                                                                                                                                       aTAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGAc                                                                                                        <  1:2319847/60‑1 (MQ=255)

                                                                                                                                       aTAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGAc                                                                                                        >  2:2319847/1‑60 (MQ=255)

                                                                                                                                       aTAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCg                                                                                        <  2:91929/76‑1 (MQ=255)

                                                                                                                                       aTAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCg                                                                                        >  1:91929/1‑76 (MQ=255)

                                                                                                                                       aTAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGc                                             <  1:1086024/119‑1 (MQ=255)

                                                                                                                                       aTAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGc                                             >  2:1086024/1‑119 (MQ=255)

                                                                                                                                       aTAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCAtt              >  2:923029/1‑150 (MQ=255)

                                                                                                                                        tAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTg             <  2:1709559/150‑1 (MQ=255)

                                                                                                                                         ccgtgaggCCGACGGCCGCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTgc            >  1:233119/7‑150 (MQ=255)

                                                                                                                                         ccgtgaggCCGACGGCCGCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTgc            >  1:155644/7‑150 (MQ=255)

                                                                                                                                         ccgTGAGGTCGACGGCCGCACGGGTCCACACCGTGCAACCCAAGTCTTTCATGGTTACCGGCTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTgc            >  1:47700/3‑150 (MQ=255)

                                                                                                                                         aGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCgggg                                                                              <  2:1997146/84‑1 (MQ=255)

                                                                                                                                         aGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCgggg                                                                              >  1:1997146/1‑84 (MQ=255)

                                                                                                                                           gtgaggCCGACGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTg             <  1:1517493/143‑1 (MQ=255)

                                                                                                                                           gtgaggCCGACGGCCGCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGATGCCCGGGGGGACCACGCGGaa                                                                 <  1:1089330/91‑1 (MQ=255)

                                                                                                                                           gTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTgcgc          >  1:1018377/1‑150 (MQ=255)

                                                                                                                                           gTGAGGCCGACGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTg             >  2:1517493/1‑147 (MQ=255)

                                                                                                                                           gTGAGGCCGACGGCCGCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGATGCCCGGGGGGACCACGCGGaa                                                                 >  2:1089330/1‑95 (MQ=255)

                                                                                                                                            tgaggCCGACGGCCGCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTGCGCt         <  1:999831/147‑1 (MQ=255)

                                                                                                                                               ggCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTGCGCTGCa      >  1:591052/1‑150 (MQ=255)

                                                                                                                                                 ccGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTGCGCTGCAtt    >  2:475404/1‑150 (MQ=255)

                                                                                                                                                  cGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCa                                         >  1:1399673/1‑112 (MQ=255)

                                                                                                                                                  cGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCa                                         <  2:1399673/112‑1 (MQ=255)

GGGGATCAGCATCGATACCGGCTGGCCGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTGCGCTGCATTTC  >  NC_002947/2410750‑2411046

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`