BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,410,904	T→G	6.7%	E196A (GAG→GCG)	acnA‑I ←	aconitate hydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,410,904	0	T	G	6.7%	298.3 / 11.8	104	E196A (GAG→GCG)	acnA‑I	aconitate hydratase
Reads supporting (aligned to +/- strand): ref base T (53/44); new base G (5/2); total (58/46)
Fisher's exact test for biased strand distribution p-value = 4.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.64e-01

Only 100 of 104 total aligned reads displayed.

GCATCGATACCGGCTGGCCGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTGCGCTGCATTTCGATG  >  NC_002947/2410758‑2411050

gCATCGATACCGGCTGGCCGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCAc                                                                                                                                                 >  2:1966430/1‑150 (MQ=255)

  aTCGATACCGGCTGGCCGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCAc                                                                                                                                                 >  2:1378780/1‑148 (MQ=255)

  aTCGATACCGGCTGGCCGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCAc                                                                                                                                                 <  1:1378780/148‑1 (MQ=255)

                  cGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGc                                                                                                                               >  1:1925094/1‑150 (MQ=255)

                  cGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGc                                                                                                                               >  2:1886129/1‑150 (MQ=255)

                  cGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGc                                                                                                                               >  2:585110/1‑150 (MQ=255)

                         ggCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCgg                                                                                                                                >  1:378191/1‑142 (MQ=255)

                         ggCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCgg                                                                                                                                <  2:378191/142‑1 (MQ=255)

                            cgcttccgcttcGATGCCGCCGACACCCCAGCCGAGTCCGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTc                                                                                                                     <  2:484713/150‑1 (MQ=255)

                            cgcttccgcttcGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTc                                                                                                                     <  1:1552184/150‑1 (MQ=255)

                                tccgcttcGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAgg                                                                                                                 <  1:829612/150‑1 (MQ=255)

                                 ccgcttcGATGCCGCCGACACCGCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGt                                                                                                                <  2:1856690/150‑1 (MQ=255)

                                     ttcGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGAc                                                                                                            >  2:1166639/1‑150 (MQ=255)

                                       cGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCt                                                                                                          <  2:367267/150‑1 (MQ=255)

                                        gATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCtg                                                                                                         <  1:40274/150‑1 (MQ=255)

                                        gATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCAGGGTGGTGCGGAAGACTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCtg                                                                                                         >  2:1791354/1‑150 (MQ=255)

                                         aTGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCTCGGGTCCAAACCGTGCGGCCCAGGTATTCCAGGTTGACCtgg                                                                                                        <  2:1077561/150‑1 (MQ=255)

                                            ccgccgACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCtggtgg                                                                                                     <  2:1864200/150‑1 (MQ=255)

                                             cgccgACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGc                                                                                                    >  1:2032944/1‑150 (MQ=255)

                                             cgccgACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGGGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGc                                                                                                    <  1:35863/150‑1 (MQ=255)

                                                     ccccAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCg                                                                                            <  2:2032944/150‑1 (MQ=255)

                                                      cccAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCtggtgg                                                                                                     >  1:1805940/1‑140 (MQ=255)

                                                      cccAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCtggtgg                                                                                                     <  2:1805940/140‑1 (MQ=255)

                                                      cccAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCgg                                                                                           >  2:1632428/1‑150 (MQ=255)

                                                      cccAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCgg                                                                                           >  1:2064431/1‑150 (MQ=255)

                                                      cccAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCAGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCgg                                                                                           >  1:19948/1‑150 (MQ=255)

                                                      cccAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGGGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTGGACCTGGTGGCAAATGCCgg                                                                                           >  1:445685/1‑150 (MQ=255)

                                                        aaGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGTGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTg                                                                                         >  2:1653998/2‑150 (MQ=255)

                                                         aGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCa                                                                                                                            >  1:1915348/1‑114 (MQ=255)

                                                             gAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCgg                                                                                    >  1:1662150/1‑150 (MQ=255)

                                                              aGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCggg                                                                                   >  1:33374/1‑150 (MQ=255)

                                                               gTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCgggg                                                                                  <  2:269379/150‑1 (MQ=255)

                                                                tACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTAGTCTAGGTTGCCCTGGTGGCAAATGCCGGTGCCCggggg                                                                                 <  1:1067521/150‑1 (MQ=255)

                                                                 aCGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCggg                                                                                   >  2:1217129/1‑147 (MQ=255)

                                                                 aCGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCggg                                                                                   <  1:1217129/147‑1 (MQ=255)

                                                                   gCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGAc                                                                              >  1:717388/1‑150 (MQ=255)

                                                                     ccAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCg                                                                                            >  2:1169154/1‑134 (MQ=255)

                                                                     ccAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCa                                                                            >  1:821108/1‑150 (MQ=255)

                                                                     ccAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGGCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCg                                                                                            <  1:1169154/134‑1 (MQ=255)

                                                                      cAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCAc                                                                           >  1:188664/1‑150 (MQ=255)

                                                                        ggCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCTACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCAcgc                                                                         <  2:605280/150‑1 (MQ=255)

                                                                               gATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGtt                                                                  >  1:194291/1‑150 (MQ=255)

                                                                                   aTGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCg                                                              <  1:358203/150‑1 (MQ=255)

                                                                                   aTGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGAAGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCg                                                              >  2:1534711/1‑150 (MQ=255)

                                                                                    tggtggTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTCACACCGAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGCGGGACCACGCGGAAGTTGTCGa                                                             >  1:1956557/1‑150 (MQ=255)

                                                                                      gtggtgTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAg                                                           <  1:288717/150‑1 (MQ=255)

                                                                                       tggtgTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAgg                                                          <  2:1192838/150‑1 (MQ=255)

                                                                                        ggtgTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGgc                                                         <  2:2064431/150‑1 (MQ=255)

                                                                                           gtgCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGct                                                      >  1:396812/1‑150 (MQ=255)

                                                                                               gAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCCGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGctctct                                                  <  2:445685/150‑3 (MQ=255)

                                                                                                aGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGTTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGc                                                 <  1:1632428/150‑1 (MQ=255)

                                                                                                aGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGc                                                 <  1:1886129/150‑1 (MQ=255)

                                                                                                     gtgCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCaa                                            <  1:1791354/150‑1 (MQ=255)

                                                                                                      tgCCCACCATGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCTAATGCGCTCTGGCCCCAAc                                           >  2:2113462/1‑150 (MQ=255)

                                                                                                      tgCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAAc                                           <  2:1925094/150‑1 (MQ=255)

                                                                                                       gCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACg                                          >  1:175930/1‑150 (MQ=255)

                                                                                                       gCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGAAGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACg                                          >  1:2000435/1‑150 (MQ=255)

                                                                                                        cccACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGc                                         >  1:890645/1‑150 (MQ=255)

                                                                                                        cccACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGc                                         >  2:2089752/1‑150 (MQ=255)

                                                                                                          caccaAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAg                                       >  1:474886/1‑150 (MQ=255)

                                                                                                             caAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGaaa                                    <  2:1662150/150‑1 (MQ=255)

                                                                                                             caAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGaaa                                    >  1:660493/1‑150 (MQ=255)

                                                                                                             caAGGTATCGGGGAAGGCATAGGTGCGGCAGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGaaa                                    <  2:19948/150‑1 (MQ=255)

                                                                                                              aaGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAg                                   >  2:1859326/1‑150 (MQ=255)

                                                                                                              aaGGTATCCGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAg                                   <  2:1939511/150‑1 (MQ=255)

                                                                                                                   aTCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATa                              <  2:175930/150‑1 (MQ=255)

                                                                                                                    tCGGGGAAGGCATAGGTGCGGCCGTCGGCCTGACGGGCTTACGCCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAg                             <  2:2000435/150‑1 (MQ=255)

                                                                                                                    tCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAg                             >  2:367163/1‑150 (MQ=255)

                                                                                                                      ggggAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGTACCAACGCAGAAAGGCATAgcg                           >  1:353852/1‑150 (MQ=255)

                                                                                                                         gAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTc                        <  2:890645/150‑1 (MQ=255)

                                                                                                                            ggCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGaa                                                                     >  2:1827113/1‑102 (MQ=255)

                                                                                                                            ggCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGaa                                                                     <  1:1827113/102‑1 (MQ=255)

                                                                                                                             gCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTGGcga                    >  1:1611055/1‑148 (MQ=255)

                                                                                                                               aTAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGAc                                                                                                            <  1:2319847/60‑1 (MQ=255)

                                                                                                                               aTAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGAc                                                                                                            >  2:2319847/1‑60 (MQ=255)

                                                                                                                               aTAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCg                                                                                            <  2:91929/76‑1 (MQ=255)

                                                                                                                               aTAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCg                                                                                            >  1:91929/1‑76 (MQ=255)

                                                                                                                               aTAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGc                                                 <  1:1086024/119‑1 (MQ=255)

                                                                                                                               aTAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGc                                                 >  2:1086024/1‑119 (MQ=255)

                                                                                                                               aTAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCAtt                  >  2:923029/1‑150 (MQ=255)

                                                                                                                                tAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTg                 <  2:1709559/150‑1 (MQ=255)

                                                                                                                                 ccgtgaggCCGACGGCCGCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTgc                >  1:155644/7‑150 (MQ=255)

                                                                                                                                 ccgtgaggCCGACGGCCGCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTgc                >  1:233119/7‑150 (MQ=255)

                                                                                                                                 ccgTGAGGTCGACGGCCGCACGGGTCCACACCGTGCAACCCAAGTCTTTCATGGTTACCGGCTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTgc                >  1:47700/3‑150 (MQ=255)

                                                                                                                                 aGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCgggg                                                                                  <  2:1997146/84‑1 (MQ=255)

                                                                                                                                   gtgaggCCGACGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTg                 <  1:1517493/143‑1 (MQ=255)

                                                                                                                                   gtgaggCCGACGGCCGCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGATGCCCGGGGGGACCACGCGGaa                                                                     <  1:1089330/91‑1 (MQ=255)

                                                                                                                                   gTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTgcgc              >  1:1018377/1‑150 (MQ=255)

                                                                                                                                   gTGAGGCCGACGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTg                 >  2:1517493/1‑147 (MQ=255)

                                                                                                                                   gTGAGGCCGACGGCCGCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGATGCCCGGGGGGACCACGCGGaa                                                                     >  2:1089330/1‑95 (MQ=255)

                                                                                                                                    tgaggCCGACGGCCGCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTGCGCt             <  1:999831/147‑1 (MQ=255)

                                                                                                                                       ggCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTGCGCTGCa          >  1:591052/1‑150 (MQ=255)

                                                                                                                                         ccGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTGCGCTGCAtt        >  2:475404/1‑150 (MQ=255)

                                                                                                                                          cGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCa                                             <  2:1399673/112‑1 (MQ=255)

                                                                                                                                          cGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCa                                             >  1:1399673/1‑112 (MQ=255)

                                                                                                                                           gTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTGCGCTGCATTTc      >  1:961553/1‑150 (MQ=255)

                                                                                                                                           gTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTGCGCTGCATTTc      >  2:2104110/1‑150 (MQ=255)

                                                                                                                                              ggCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTGCGCTGCATTTCGAt   <  2:717388/150‑1 (MQ=255)

                                                                                                                                              ggCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTGCGCTGCATTTCGAt   >  2:1896780/1‑150 (MQ=255)

                                                                                                                                               gCCTCACGGGTCCACACCGTGCGGCCCAGGTATTACAGGTTGACCTGGTGGCAAATGGCTGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTGCGCTGCATTTCGATg  >  1:297747/1‑150 (MQ=255)

GCATCGATACCGGCTGGCCGAGCATGGCCGCTTCCGCTTCGATGCCGCCGACACCCCAGCCGAGTACGCCCAGGCCGTTGATCATGGTGGTGTGCGAGTCTGTGCCCACCAAGGTATCGGGGAAGGCATAGGTGCGGCCGTCGGCCTCACGGGTCCACACCGTGCGGCCCAGGTATTCCAGGTTGACCTGGTGGCAAATGCCGGTGCCCGGGGGGACCACGCGGAAGTTGTCGAAGGCGCTCTGGCCCCAACGCAGAAAGGCATAGCGCTCGCCATTGCGCTGCATTTCGATG  >  NC_002947/2410758‑2411050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`