Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,442,711	G→T	6.9%	P602P (CCG→CCT)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,442,711	0	G	T	6.9%	272.7 / 7.6	87	P602P (CCG→CCT)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base G (43/38);  new base T (3/3);  total (46/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.06e-01

AAGAGAACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAGGG  >  NC_002947/2442563‑2442860                                                                                                                                                     |                                                                                                                                                       aaGAGAACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGt                                                                                                                                                       <  1:1868679/150‑1 (MQ=255)  agagAACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTg                                                                                                                                                      <  1:255096/150‑1 (MQ=255)     gaACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCaa                                                                                                                                                   <  1:1070472/150‑1 (MQ=255)     gaACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCaa                                                                                                                                                   >  1:729086/1‑150 (MQ=255)         cTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGaa                                                                                                                                               >  2:1513310/1‑150 (MQ=255)             acgacgTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAAtg‑tg                                                                                                                                          >  1:383051/1‑150 (MQ=255)              cgacgTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑Tgg                                                                                                                                         <  1:1513310/150‑1 (MQ=255)                acgTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTc                                                                                                                                       <  1:292411/150‑1 (MQ=255)                acgTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACC‑ACATTCCTTGCACGGAAtg‑ttggtc                                                                                                                                      <  1:135808/150‑6 (MQ=255)                  gTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCgg                                                                                                                                     >  1:1007922/1‑150 (MQ=255)                  gTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCgg                                                                                                                                     >  2:707455/1‑150 (MQ=255)                      ccctgggCGA‑CGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgat                                                                                                                                <  1:832040/146‑1 (MQ=255)                      cccAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCga                                                                                                                                 >  1:2003631/1‑150 (MQ=255)                       ccAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgat                                                                                                                                >  2:975673/1‑150 (MQ=255)                       ccAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgat                                                                                                                                >  2:866188/1‑150 (MQ=255)                           ggCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgatgatg                                                                                                                            <  2:2003631/150‑1 (MQ=255)                               aGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAAAATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTc                                                                                                                        <  1:1259563/150‑1 (MQ=255)                                gCGTGACTGGAAGAAAGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCg                                                                                                                       <  2:959468/150‑1 (MQ=255)                                       tGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGt                                                                                                                                                       >  2:357575/1‑112 (MQ=255)                                       tGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGt                                                                                                                                                       <  1:357575/112‑1 (MQ=255)                                       tGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCAc                                                                                                                    <  2:2279363/146‑1 (MQ=255)                                       tGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCAc                                                                                                                    >  1:2279363/1‑146 (MQ=255)                                              aCGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACgg                                                                                                         <  2:1293204/150‑1 (MQ=255)                                                gTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGgt                                                                                                       <  2:1007922/150‑1 (MQ=255)                                                 tGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGgtg                                                                                                      >  2:680477/1‑150 (MQ=255)                                                  gCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGgtgt                                                                                                     >  2:1620536/1‑150 (MQ=255)                                                       acgaGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGt                                                                                                                                                       <  1:347951/96‑1 (MQ=255)                                                       acgaGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGt                                                                                                                                                       >  2:347951/1‑96 (MQ=255)                                                           gTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTggg                                                                                            >  2:2038498/1‑150 (MQ=255)                                                                 cGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACgg                                                                                                         <  1:2279587/131‑1 (MQ=255)                                                                 cGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACgg                                                                                                         >  2:2279587/1‑131 (MQ=255)                                                                  ggCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgat                                                                                                                                >  1:1882804/1‑107 (MQ=255)                                                                  ggCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgat                                                                                                                                <  2:1882804/107‑1 (MQ=255)                                                                  ggCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGCTGATGATTAGCACCGCTTCTACAGCGGTGTTCCTGGGTTGctgt                                                                                     >  2:1127814/1‑148 (MQ=255)                                                                  ggCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGctct                                                                                     >  1:842360/1‑150 (MQ=255)                                                                   gCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTg                                                                                    <  2:1770078/150‑1 (MQ=255)                                                                    cGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTgg                                                                                   <  2:383051/150‑1 (MQ=255)                                                                       tttCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTa                                                                                <  2:805169/150‑1 (MQ=255)                                                                            gCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGcc                                                                           >  2:612948/1‑150 (MQ=255)                                                                              aaaaaGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTg                                                                         <  1:1883185/150‑1 (MQ=255)                                                                              aaaaaGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTg                                                                         >  2:1763166/1‑150 (MQ=255)                                                                              aaaaaGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTg                                                                         >  1:400166/1‑150 (MQ=255)                                                                               ccagagcaacccaggaacacacccgtagaagcggtgcgaatcatcatcgGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgatg                                                                                                                               <  2:201330‑M1/46‑1 (MQ=255)                                                                               ccagagcaacccaggaacacacccgtagaagcggtgcgaatcatcatcgGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgatg                                                                                                                               >  1:201330‑M1/50‑95 (MQ=255)                                                                                 agagcaacccaggaacacacccgtagaagcggtgcgaatcatcatcgGCCGACCATGACCAACATTCCGTGCAAGGAATG‑Tgg                                                                                                                                         >  1:1522381‑M1/48‑83 (MQ=255)                                                                                 agagcaacccaggaacacacccgtagaagcggtgcgaatcatcatcgGCCGACCATGACCAACATTCCGTGCAAGGAATG‑Tgg                                                                                                                                         <  2:1522381‑M1/36‑1 (MQ=255)                                                                                       cAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGCGTGTGTGCCTGGGTTTCTCTGGCTACAGCCTGCCGCCGaaa                                                                <  2:2081467/150‑1 (MQ=255)                                                                                         gACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGTATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAaga                                                              <  1:1620536/150‑1 (MQ=255)                                                                                            tGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCAc                                                                                                                    <  2:383020/93‑1 (MQ=255)                                                                                            tGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCAc                                                                                                                    >  1:383020/1‑93 (MQ=255)                                                                                                   tCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAg                                                    <  2:400166/150‑1 (MQ=255)                                                                                                   tCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAg                                                    <  2:729086/150‑1 (MQ=255)                                                                                                      aGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGACt                                                 >  2:1684453/1‑150 (MQ=255)                                                                                                       gCGAAGATGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTa                                                >  2:29988/1‑150 (MQ=255)                                                                                                         gcaGACGGCATGCGTGCCAACCAGCCCACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCTCCGAAAGAGCGCTGCAAGGCTAcc                                              <  1:1874442/148‑1 (MQ=255)                                                                                                             aCGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCa                                          >  1:2158546/1‑150 (MQ=255)                                                                                                                      gTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGc                                 <  1:37615/150‑1 (MQ=255)                                                                                                                        gCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCg                               >  1:602236/1‑150 (MQ=255)                                                                                                                         ccAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCgg                              >  2:1539963/1‑150 (MQ=255)                                                                                                                           aaCCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TTGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGc                            >  1:607063/1‑150 (MQ=255)                                                                                                                           aaCCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCaa                                         <  2:2203326/137‑1 (MQ=255)                                                                                                                           aaCCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCaa                                         >  1:2203326/1‑137 (MQ=255)                                                                                                                           aaCCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGc                            >  1:1452552/1‑150 (MQ=255)                                                                                                                                  CGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACgaga                     >  1:707455‑M1/1‑150 (MQ=255)                                                                                                                                    ACCATGACCAACATTCCGTGCAAGGAATG‑TTGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGAtt                   >  2:607063‑M1/1‑150 (MQ=255)                                                                                                                                    ACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGctct                                                                                     <  2:2676‑M1/84‑1 (MQ=255)                                                                                                                                    ACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGctct                                                                                     >  1:2676‑M1/1‑84 (MQ=255)                                                                                                                                    ACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCt                                                                          >  2:1603667‑M1/1‑95 (MQ=255)                                                                                                                                    ACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCt                                                                          <  1:1603667‑M1/95‑1 (MQ=255)                                                                                                                                    ACCATGACCAACATCCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGAtt                   >  1:866188‑M1/1‑150 (MQ=255)                                                                                                                                     CCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGGGATTg                  >  1:612948‑M1/1‑150 (MQ=255)                                                                                                                                        tGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCg               <  1:608993/150‑1 (MQ=255)                                                                                                                                          gaccaCATTCCTTGCACGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGc              >  1:478320/5‑149 (MQ=255)                                                                                                                                          gaccaCATTCCTTGCACGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGc              <  2:478320/145‑1 (MQ=255)                                                                                                                                          gaccaCATTCCTTGCACGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCt             >  2:2297338/5‑150 (MQ=255)                                                                                                                                           ccAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGCGTTCCTGGGTTGctct                                                                                     >  2:756554/1‑77 (MQ=255)                                                                                                                                           ccAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGCGTTCCTGGGTTGctct                                                                                     <  1:756554/77‑1 (MQ=255)                                                                                                                                             caCATTCCTTGCACGGAATGTTGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCaa                                         <  1:1035037/119‑1 (MQ=255)                                                                                                                                             caCATTCCTTGCACGGAATGTTGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCaa                                         >  2:1035037/2‑120 (MQ=255)                                                                                                                                             aaCATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTgac          >  1:1674690/1‑150 (MQ=255)                                                                                                                                             aaCATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTgac          >  1:273171/1‑150 (MQ=255)                                                                                                                                                 ttCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGacgacg      >  1:613504/1‑150 (MQ=255)                                                                                                                                                 ttCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGacgacg      >  1:175278/1‑150 (MQ=255)                                                                                                                                                  tCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGAcgacga     <  1:680477/150‑1 (MQ=255)                                                                                                                                                   ccGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAg    <  2:842360/150‑1 (MQ=255)                                                                                                                                                    cGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAgg   <  1:225793/150‑1 (MQ=255)                                                                                                                                                    cGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTAAAGTGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAgg   <  1:1233295/150‑1 (MQ=255)                                                                                                                                                     gTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAggg  >  2:2230207/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                       AAGAGAACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAGGG  >  NC_002947/2442563‑2442860

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑