Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,442,716	A→C	7.3%	K604T (AAG→ACG)	topA →	DNA topoisomerase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,442,716	0	A	C	7.3%	229.5 / 9.9	82	K604T (AAG→ACG)	topA	DNA topoisomerase I
Reads supporting (aligned to +/- strand):  ref base A (42/34);  new base C (3/3);  total (45/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-01

GAACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAGGGCGAGT  >  NC_002947/2442567‑2442865                                                                                                                                                      |                                                                                                                                                       gaACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCaa                                                                                                                                                        <  1:1070472/150‑1 (MQ=255) gaACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCaa                                                                                                                                                        >  1:729086/1‑150 (MQ=255)     cTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGaa                                                                                                                                                    >  2:1513310/1‑150 (MQ=255)         acgacgTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAAtg‑tg                                                                                                                                               >  1:383051/1‑150 (MQ=255)          cgacgTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑Tgg                                                                                                                                              <  1:1513310/150‑1 (MQ=255)            acgTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTc                                                                                                                                            <  1:292411/150‑1 (MQ=255)            acgTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACC‑ACATTCCTTGCACGGAAtg‑ttggtc                                                                                                                                           <  1:135808/150‑6 (MQ=255)              gTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCgg                                                                                                                                          >  1:1007922/1‑150 (MQ=255)              gTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCgg                                                                                                                                          >  2:707455/1‑150 (MQ=255)                  ccctgggCGA‑CGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgat                                                                                                                                     <  1:832040/146‑1 (MQ=255)                  cccAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCga                                                                                                                                      >  1:2003631/1‑150 (MQ=255)                   ccAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgat                                                                                                                                     >  2:975673/1‑150 (MQ=255)                   ccAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgat                                                                                                                                     >  2:866188/1‑150 (MQ=255)                       ggCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgatgatg                                                                                                                                 <  2:2003631/150‑1 (MQ=255)                           aGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAAAATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTc                                                                                                                             <  1:1259563/150‑1 (MQ=255)                            gCGTGACTGGAAGAAAGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCg                                                                                                                            <  2:959468/150‑1 (MQ=255)                                   tGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCAc                                                                                                                         <  2:2279363/146‑1 (MQ=255)                                   tGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCAc                                                                                                                         >  1:2279363/1‑146 (MQ=255)                                          aCGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACgg                                                                                                              <  2:1293204/150‑1 (MQ=255)                                            gTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGgt                                                                                                            <  2:1007922/150‑1 (MQ=255)                                             tGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGgtg                                                                                                           >  2:680477/1‑150 (MQ=255)                                              gCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGgtgt                                                                                                          >  2:1620536/1‑150 (MQ=255)                                                       gTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTggg                                                                                                 >  2:2038498/1‑150 (MQ=255)                                                             cGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACgg                                                                                                              >  2:2279587/1‑131 (MQ=255)                                                             cGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACgg                                                                                                              <  1:2279587/131‑1 (MQ=255)                                                              ggCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgat                                                                                                                                     <  2:1882804/107‑1 (MQ=255)                                                              ggCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgat                                                                                                                                     >  1:1882804/1‑107 (MQ=255)                                                              ggCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGCTGATGATTAGCACCGCTTCTACAGCGGTGTTCCTGGGTTGctgt                                                                                          >  2:1127814/1‑148 (MQ=255)                                                              ggCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGctct                                                                                          >  1:842360/1‑150 (MQ=255)                                                               gCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTg                                                                                         <  2:1770078/150‑1 (MQ=255)                                                                cGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTgg                                                                                        <  2:383051/150‑1 (MQ=255)                                                                   tttCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTa                                                                                     <  2:805169/150‑1 (MQ=255)                                                                        gCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGcc                                                                                >  2:612948/1‑150 (MQ=255)                                                                          aaaaaGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTg                                                                              >  1:400166/1‑150 (MQ=255)                                                                          aaaaaGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTg                                                                              >  2:1763166/1‑150 (MQ=255)                                                                          aaaaaGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTg                                                                              <  1:1883185/150‑1 (MQ=255)                                                                           ccagagcaacccaggaacacacccgtagaagcggtgcgaatcatcatcgGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgatg                                                                                                                                    >  1:201330‑M1/50‑95 (MQ=255)                                                                           ccagagcaacccaggaacacacccgtagaagcggtgcgaatcatcatcgGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCgatg                                                                                                                                    <  2:201330‑M1/46‑1 (MQ=255)                                                                             agagcaacccaggaacacacccgtagaagcggtgcgaatcatcatcgGCCGACCATGACCAACATTCCGTGCAAGGAATG‑Tgg                                                                                                                                              <  2:1522381‑M1/36‑1 (MQ=255)                                                                             agagcaacccaggaacacacccgtagaagcggtgcgaatcatcatcgGCCGACCATGACCAACATTCCGTGCAAGGAATG‑Tgg                                                                                                                                              >  1:1522381‑M1/48‑83 (MQ=255)                                                                                   cAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGCGTGTGTGCCTGGGTTTCTCTGGCTACAGCCTGCCGCCGaaa                                                                     <  2:2081467/150‑1 (MQ=255)                                                                                     gACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGTATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAaga                                                                   <  1:1620536/150‑1 (MQ=255)                                                                                        tGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCAc                                                                                                                         >  1:383020/1‑93 (MQ=255)                                                                                        tGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCAc                                                                                                                         <  2:383020/93‑1 (MQ=255)                                                                                               tCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAg                                                         <  2:729086/150‑1 (MQ=255)                                                                                               tCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAg                                                         <  2:400166/150‑1 (MQ=255)                                                                                                  aGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGACt                                                      >  2:1684453/1‑150 (MQ=255)                                                                                                   gCGAAGATGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTa                                                     >  2:29988/1‑150 (MQ=255)                                                                                                     gcaGACGGCATGCGTGCCAACCAGCCCACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCTCCGAAAGAGCGCTGCAAGGCTAcc                                                   <  1:1874442/148‑1 (MQ=255)                                                                                                         aCGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCa                                               >  1:2158546/1‑150 (MQ=255)                                                                                                                  gTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGc                                      <  1:37615/150‑1 (MQ=255)                                                                                                                    gCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCg                                    >  1:602236/1‑150 (MQ=255)                                                                                                                     ccAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCgg                                   >  2:1539963/1‑150 (MQ=255)                                                                                                                       aaCCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TTGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGc                                 >  1:607063/1‑150 (MQ=255)                                                                                                                       aaCCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCaa                                              >  1:2203326/1‑137 (MQ=255)                                                                                                                       aaCCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCaa                                              <  2:2203326/137‑1 (MQ=255)                                                                                                                       aaCCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGc                                 >  1:1452552/1‑150 (MQ=255)                                                                                                                              CGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACgaga                          >  1:707455‑M1/1‑150 (MQ=255)                                                                                                                                ACCATGACCAACATTCCGTGCAAGGAATG‑TTGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGAtt                        >  2:607063‑M1/1‑150 (MQ=255)                                                                                                                                ACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGctct                                                                                          >  1:2676‑M1/1‑84 (MQ=255)                                                                                                                                ACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGctct                                                                                          <  2:2676‑M1/84‑1 (MQ=255)                                                                                                                                ACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCt                                                                               >  2:1603667‑M1/1‑95 (MQ=255)                                                                                                                                ACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCt                                                                               <  1:1603667‑M1/95‑1 (MQ=255)                                                                                                                                ACCATGACCAACATCCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGAtt                        >  1:866188‑M1/1‑150 (MQ=255)                                                                                                                                 CCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGGGATTg                       >  1:612948‑M1/1‑150 (MQ=255)                                                                                                                                    tGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCg                    <  1:608993/150‑1 (MQ=255)                                                                                                                                      gaccaCATTCCTTGCACGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGc                   <  2:478320/145‑1 (MQ=255)                                                                                                                                      gaccaCATTCCTTGCACGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGc                   >  1:478320/5‑149 (MQ=255)                                                                                                                                      gaccaCATTCCTTGCACGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCt                  >  2:2297338/5‑150 (MQ=255)                                                                                                                                       ccAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGCGTTCCTGGGTTGctct                                                                                          <  1:756554/77‑1 (MQ=255)                                                                                                                                       ccAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGCGTTCCTGGGTTGctct                                                                                          >  2:756554/1‑77 (MQ=255)                                                                                                                                         caCATTCCTTGCACGGAATGTTGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCaa                                              <  1:1035037/119‑1 (MQ=255)                                                                                                                                         caCATTCCTTGCACGGAATGTTGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCaa                                              >  2:1035037/2‑120 (MQ=255)                                                                                                                                         aaCATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTgac               >  1:1674690/1‑150 (MQ=255)                                                                                                                                         aaCATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTgac               >  1:273171/1‑150 (MQ=255)                                                                                                                                             ttCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGacgacg           >  1:613504/1‑150 (MQ=255)                                                                                                                                             ttCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGacgacg           >  1:175278/1‑150 (MQ=255)                                                                                                                                              tCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGAcgacga          <  1:680477/150‑1 (MQ=255)                                                                                                                                               ccGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAg         <  2:842360/150‑1 (MQ=255)                                                                                                                                                cGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAgg        <  1:225793/150‑1 (MQ=255)                                                                                                                                                cGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTAAAGTGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAgg        <  1:1233295/150‑1 (MQ=255)                                                                                                                                                 gTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAggg       >  2:2230207/1‑150 (MQ=255)                                                                                                                                                    cAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGctct                                                                                          >  2:2291815/1‑64 (MQ=255)                                                                                                                                                    cAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGctct                                                                                          <  1:2291815/64‑1 (MQ=255)                                                                                                                                                      aGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAGGGCGAGt  <  1:2038498/150‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                       GAACCTCGACGACGTCGCCCAGGGCGAGCGTGACTGGAAGAACGTGCTCGACGAGTTCTACGGCGATTTCAGCAAAAAGCTGCAGACTGCCGAGTCCAGCGAAGACGGCATGCGTGCCAACCAGCCGACCATGACCAACATTCCGTGCAAGGAATG‑TGGTCGGCCGATGATGATTCGCACCGCTTCTACGGGTGTGTTCCTGGGTTGCTCTGGCTACAGCCTGCCGCCGAAAGAGCGCTGCAAGGCTACCGTCAACCTGGTGCCGGGCGACGAGATTGCCGCTGACGACGAGGGCGAGT  >  NC_002947/2442567‑2442865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑