Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,461,778 A→T 10.7% Q245L (CAG→CTG)  lolC → lipoprotein releasing system transmembrane protein LolC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,461,7780AT10.7% 266.6 / 21.4 96Q245L (CAG→CTG) lolClipoprotein releasing system transmembrane protein LolC
Reads supporting (aligned to +/- strand):  ref base A (41/42);  new base T (5/5);  total (48/48)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.85e-01

Only 100 of 102 total aligned reads displayed.

GGGGAGATCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCGTGGCGGTGGCGGCGTTCAACATCATTTCCACCCTGG  >  NC_002947/2461633‑2461924
                                                                                                                                                 |                                                                                                                                                  
ggggAGATCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCCCCCACCAGCTg                                                                                                                                                <  2:1198391/150‑1 (MQ=255)
 gggAGATCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTgg                                                                                                                                               <  1:300387/150‑1 (MQ=255)
 gggAGATCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCCCGCGTAGCCTGGAGCATCGCCCAGCAGCTgg                                                                                                                                               <  2:1484179/150‑1 (MQ=255)
   gagaTCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGc                                                                                                                                             <  2:1968208/150‑1 (MQ=255)
     gaTCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCTGCTGGGCGa                                                                                                                                           <  2:83465/150‑1 (MQ=255)
      aTCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCTGCTGGGCGat                                                                                                                                          <  2:1403455/150‑2 (MQ=255)
       tCGACTGCTACCTGGGCCTGCCCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGatg                                                                                                                                         <  2:621772/150‑3 (MQ=255)
       tCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCGGCTGGGCGAcc                                                                                                                                         >  1:1917820/1‑150 (MQ=255)
         gACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAg                                                                                                                                       <  2:1302824/150‑1 (MQ=255)
         gACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGACTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAg                                                                                                                                       >  1:1020993/1‑150 (MQ=255)
          aCGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCCGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAATTCGACCACCTGTTCCAGGCGCCGCGCGTAGCCTGACGCATAGCCCAGCGGCCCGGTGACCacg                                                                                                                                      >  2:750207/1‑148 (MQ=255)
                  cTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCtat                                                                                                                              <  1:1063206/150‑1 (MQ=255)
                  cTGGGCCTGACCAACATCAGCGACCTGTCCAGCCTTCACCTCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCtat                                                                                                                              >  1:665876/1‑150 (MQ=255)
                     ggCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGc                                                                                                                           >  1:788108/1‑150 (MQ=255)
                      gCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTgg                                                                                                                                               <  1:1530161/129‑1 (MQ=255)
                      gCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTgg                                                                                                                                               >  2:1530161/1‑129 (MQ=255)
                       ccTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCta                                                                                                                               <  1:1722185/144‑1 (MQ=255)
                       ccTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCta                                                                                                                               >  2:1722185/1‑144 (MQ=255)
                             cAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCTCCCAGCAGCTGGGCGACCAGGCATTCTATAGCCGTGACTg                                                                                                                   >  2:496435/1‑150 (MQ=255)
                              aaCATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTgg                                                                                                                  >  1:1653614/1‑150 (MQ=255)
                                cATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACt                                                                                                                    >  2:1427488/1‑146 (MQ=255)
                                cATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACt                                                                                                                    <  1:1427488/146‑1 (MQ=255)
                                cATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGAc                                                                                                                <  2:420960/150‑1 (MQ=255)
                                  tCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGAccc                                                                                                              >  1:254388/1‑150 (MQ=255)
                                     gCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTa                                                                                                           <  1:1575050/150‑1 (MQ=255)
                                      cGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTAc                                                                                                          <  1:2392964/150‑1 (MQ=255)
                                       gACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCGTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCa                                                                                                                                        >  1:2357645/1‑119 (MQ=255)
                                       gACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCa                                                                                                                                        <  2:2357645/119‑1 (MQ=255)
                                         ccTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCa                                                                                                       <  1:1317510/150‑1 (MQ=255)
                                          cTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCAc                                                                                                      >  1:2049282/1‑150 (MQ=255)
                                               ccGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGc                                                                                                                           >  2:774218/1‑124 (MQ=255)
                                               ccGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGc                                                                                                                           <  1:774218/124‑1 (MQ=255)
                                               ccGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCaa                                                                                                 >  2:53745/1‑150 (MQ=255)
                                               ccGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCaa                                                                                                 >  1:945386/1‑150 (MQ=255)
                                                cGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGTACCCGTACCCACGGCAAc                                                                                                >  2:1748174/1‑150 (MQ=255)
                                                  catGCACCGCTGGAAGCCCGACCAGGTGCAGGGCCGGCGCCTGAAGTTCAACCACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCGCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCt                                                                                              <  1:750207/148‑1 (MQ=255)
                                                   cTGCACCGCTAGAAGCCCGACCAAGTCCAGGGCCTGCGCCTGAAGTTCGACTACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAAGTCTATAGCCTTGGCTGGACCCGTACCCACGGCAACCTg                                                                                             >  2:759486/1‑150 (MQ=255)
                                                    tGCACCGCTGGCAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCt                                                                                                                                <  1:891891/114‑1 (MQ=255)
                                                    tGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCt                                                                                                                                >  2:891891/1‑114 (MQ=255)
                                                     gCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCcagcag                                                                                                                                                   >  1:304868/1‑94 (MQ=255)
                                                     gCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCcagcag                                                                                                                                                   <  2:304868/94‑1 (MQ=255)
                                                       aCCGCTGGAAGCCCGCCAAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTATAcc                                                                                         >  2:953531/1‑150 (MQ=255)
                                                        ccGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCa                                                                                        >  2:2033822/1‑150 (MQ=255)
                                                         cGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCcagcag                                                                                                                                                   >  2:1516539/1‑90 (MQ=255)
                                                         cGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCcagcag                                                                                                                                                   <  1:1516539/90‑1 (MQ=255)
                                                         cGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACAAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAg                                                                                       <  2:945386/150‑1 (MQ=255)
                                                         cGCTGGAAGAACGACTATGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAg                                                                                       <  2:61449/150‑1 (MQ=255)
                                                            tGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCta                                                                                                                               <  2:305928/107‑1 (MQ=255)
                                                            tGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCta                                                                                                                               >  1:305928/1‑107 (MQ=255)
                                                             ggAAGCCCGCCCAGGTCCAGGGCCTGCGCCTGAATTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGa                                                                                   <  1:2221752/150‑1 (MQ=255)
                                                              gAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTAc                                                                                                          <  1:1261532/126‑1 (MQ=255)
                                                              gAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTAc                                                                                                          >  2:1261532/1‑126 (MQ=255)
                                                                    cGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTAt                                                                            >  2:402389/1‑150 (MQ=255)
                                                                          ggTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGaaaa                                                                      <  2:1806734/150‑1 (MQ=255)
                                                                           gTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAg                                                                     >  1:1878376/1‑150 (MQ=255)
                                                                              cAGGGCTTGCGCCGGAAGTTCGTCGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGcc                                                                  <  1:2081419/150‑1 (MQ=255)
                                                                              cAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGAc                                                                                                                     >  2:2111365/1‑99 (MQ=255)
                                                                              cAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGAc                                                                                                                     <  1:2111365/99‑1 (MQ=255)
                                                                              cAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGcc                                                                  <  2:665876/150‑1 (MQ=255)
                                                                               aGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGccc                                                                 <  2:1653614/150‑2 (MQ=255)
                                                                               aGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCa                                                                 <  2:1028430/150‑1 (MQ=255)
                                                                                gggCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTCCCAGGCGATCCGTATGGAAAAGGCCAt                                                                <  2:2049282/150‑1 (MQ=255)
                                                                                 ggCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATg                                                               >  1:818379/1‑150 (MQ=255)
                                                                                 ggCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACGGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGAATGGAAAAGGCCagg                                                               >  2:612924/1‑148 (MQ=255)
                                                                                      gcgcCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCgg                                                          <  2:1020993/150‑1 (MQ=255)
                                                                                            gAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCg                                                           >  2:123569/1‑143 (MQ=255)
                                                                                            gAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCg                                                           <  1:123569/143‑1 (MQ=255)
                                                                                            gAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCctgct                                                    >  1:743645/1‑150 (MQ=255)
                                                                                                ttCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTtg                                                >  1:950368/1‑150 (MQ=255)
                                                                                                              ccAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCgtggc                                  >  2:437401/1‑150 (MQ=255)
                                                                                                                aGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCgtggcgg                                >  1:950189/1‑150 (MQ=255)
                                                                                                                aGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCgtggcgg                                <  1:437401/150‑1 (MQ=255)
                                                                                                                           gTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATg                                                                           >  1:806445/1‑96 (MQ=255)
                                                                                                                           gTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATg                                                                           <  2:806445/96‑1 (MQ=255)
                                                                                                                           gTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATc                                                            <  2:1968357/111‑1 (MQ=255)
                                                                                                                           gTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATc                                                            >  1:1968357/1‑111 (MQ=255)
                                                                                                                            tAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATcc                                                                                >  1:2240546/1‑90 (MQ=255)
                                                                                                                            tAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATcc                                                                                <  2:2240546/90‑1 (MQ=255)
                                                                                                                              gCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCctg                                                      >  1:1565499/1‑114 (MQ=255)
                                                                                                                              gCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCctg                                                      <  2:1565499/114‑1 (MQ=255)
                                                                                                                                cTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCGTGGCGGTGGCGGCGTTCAAcat                >  2:1305586/1‑150 (MQ=255)
                                                                                                                                  ggAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCGTGGCGGTGGCGGCGTTCAAcatc               <  2:715202/149‑1 (MQ=255)
                                                                                                                                  ggAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCGTGGCGGTGGCGGCGTTCAAcatc               >  1:715202/1‑149 (MQ=255)
                                                                                                                                  ggAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCGGGGCGGTGGCGGCGTTCAAcatca              <  1:53745/150‑1 (MQ=255)
                                                                                                                                  ggAGCATCGCCCAGAAGCTGGGCGACCAGGAATTCTAGAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAACGCCATGATCGGCCTGCTGTTGGTGCTTATCGTGGGGGGGGCGGCGTTCAAcctca              >  2:1163172/1‑150 (MQ=255)
                                                                                                                                    ctggtCGCCCAGCTGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCGTGGCGGTGGCGGCGTTCAACATCAtt            >  2:2381152/5‑150 (MQ=255)
                                                                                                                                    aGCATCGCCCAGCGGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCGTGGCGGTGGCGGCGTTCAACATCAtt            <  2:1917820/150‑1 (MQ=255)
                                                                                                                                     tggtCGCCCAGCTGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCgt                                     >  1:2037790/4‑124 (MQ=255)
                                                                                                                                     tggtCGCCCAGCTGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCgt                                     <  2:2037790/121‑1 (MQ=255)
                                                                                                                                      cATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCGTGGCGGTGGCGGCGTTCAACATCATTTc          >  2:796077/1‑150 (MQ=255)
                                                                                                                                       gtCGCCCAGCTGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTAt                                                                            <  1:1662329/82‑1 (MQ=255)
                                                                                                                                       gtCGCCCAGCTGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTAt                                                                            >  2:1662329/2‑83 (MQ=255)
                                                                                                                                       gtCGCCCAGCTGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCGTGGCGGTGGCGGCGTTCAACATCATTTcc         >  2:375918/2‑150 (MQ=255)
                                                                                                                                        tCGCCCAGCTGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGtt                                                 >  2:1337831/1‑109 (MQ=255)
                                                                                                                                        tCGCCCAGCTGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGtt                                                 <  1:1337831/109‑1 (MQ=255)
                                                                                                                                        tCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAgg                                                                    >  2:11409/1‑90 (MQ=255)
                                                                                                                                        tCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAgg                                                                    <  1:11409/90‑1 (MQ=255)
                                                                                                                                        tCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGTCCATGATCGGCCTGCTGTTGCTGCTGATCGTGGCGGTGGCGGCGTTCAACATCATTTCCa        >  2:1361946/1‑150 (MQ=255)
                                                                                                                                                cagcTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCGTGGCGGTGGCGGCGTTCAACATCATTTCCACCCTgg  <  1:1651777/148‑1 (MQ=255)
                                                                                                                                                cagcTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCGTGGCGGTGGCGGCGTTCAACATCATTTCCACCCTgg  >  2:1651777/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
GGGGAGATCGACGGCTACCTGGGCCTGACCAACATCAGCGACCTGTCCCGCCTGCACCGCTGGAAGCCCGACCAGGTCCAGGGCCTGCGCCTGAAGTTCGACGACCTGTTCCAGGCGCCGCGCGTAGCCTGGAGCATCGCCCAGCAGCTGGGCGACCAGGAATTCTATAGCCGTGACTGGACCCGTACCCACGGCAACCTGTACCAGGCGATCCGTATGGAAAAGGCCATGATCGGCCTGCTGTTGCTGCTGATCGTGGCGGTGGCGGCGTTCAACATCATTTCCACCCTGG  >  NC_002947/2461633‑2461924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑