Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,698,550	G→A	7.8%	D235D (GAC→GAT) ‡	PP_2365 ←	AraC family transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,698,550	0	G	A	7.8%	174.0 / 2.5	68	D235D (GAC→GAT) ‡	PP_2365	AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base G (37/21);  new base A (3/2);  total (41/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.57e-01

GCAAGAACAGGCAGGGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCTTTTC  >  NC_002947/2698403‑2698693                                                                                                                                                     |                                                                                                                                                 gCAAGAACAGGCAGGGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTc                                                                                                                                                >  1:319124/1‑150 (MQ=255)  cAAGAACAGGCAGGGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTTAACATGCTGGCGAAGG‑CACTTGGGCTCTCa                                                                                                                                               >  2:1189649/1‑150 (MQ=255)  cAAGAACAGGCAGGGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCa                                                                                                                                               <  2:1138969/150‑1 (MQ=255)     gAACAGGCAGGGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAg                                                                                                                                            <  1:1070560/150‑1 (MQ=255)      aaCAGGCAGGGCGAGCCGTCAGCGGAAAGCCAGTAAGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGc                                                                                                                                           <  2:759935/150‑1 (MQ=255)        caggcaggGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGccc                                                                                                                                         <  2:2046341/150‑1 (MQ=255)          ggcaggGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCaa                                                                                                                                       <  1:2127534/150‑1 (MQ=255)           gcaggGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAg                                                                                                                                      <  2:2123465/150‑1 (MQ=255)           gcaggGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAg                                                                                                                                      >  1:1679686/1‑150 (MQ=255)           gcaggGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAg                                                                                                                                      >  2:2379871/1‑150 (MQ=255)                      cGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCcac                                                                                                                          >  1:2070752/1‑150 (MQ=255)                        tCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACAt                                                                                                                        >  2:1448238/1‑150 (MQ=255)                           gCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCga                                                                                                                     >  2:1018589/1‑150 (MQ=255)                           gCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGCGCGTGGTACCGAACTGGCGCTTGCACATGCTGGCGAACG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCga                                                                                                                     >  2:1018761/1‑150 (MQ=255)                            ggaaCAGCCCGTATGGGGGGGGCCAGTGGGGGAAAATGGGTGGATTTCAGTTTATTTGAAAAATTGAGTGGGGGTGTTACCGAACTGGCGTTTGAACATGCTGGCGAAGG‑CATTGGGGCTTCAATAGCCCAGC‑GCCCCCGCCACATCgat                                                                                                                    <  2:1731809/147‑1 (MQ=255)                             ggACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCgatg                                                                                                                   >  1:282150/1‑150 (MQ=255)                              gACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCaa                                                                                                                                       >  2:1731601/1‑130 (MQ=255)                              gACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCaa                                                                                                                                       <  1:1731601/130‑1 (MQ=255)                                 aGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGGGGACTTCAGGGTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATtc                                                                                                               >  1:1862928/1‑150 (MQ=255)                                   cccGTATGCGGCGGGCCCGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATtcgc                                                                                                             >  2:2021976/1‑150 (MQ=255)                                    ccGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATtcgct                                                                                                            >  1:1008583/1‑150 (MQ=255)                                          gcggcgGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCa                                                                                                      >  2:52255/1‑150 (MQ=255)                                               gggCCAGTGCCGGCAAATGCGTGGACTTCGGTTTATCTGAAAAACTGACTGGGCGTGGTACCGAACTGGCGCTTAAACATGCTGGGGAAGG‑CACTTGGGCTGTCATAGCCAAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCa                                                                                                 <  1:2379871/150‑1 (MQ=255)                                                 gCCAGTGCCGGAAAATGCGTGGACTTCAGTTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAGGG‑CACTGGGGCTGTCATAGCCCAGG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAAt                                                                                               <  1:1448238/150‑1 (MQ=255)                                                           gCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGt                                                                                     >  2:1710922/1‑150 (MQ=255)                                                               aTGCGTGGACTTCAGGTTATCGGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCGGGCGAAGG‑CACTTGGCCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCt                                                                                 <  1:582978/150‑1 (MQ=255)                                                                   gTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGc                                                                                                                             <  2:597297/102‑1 (MQ=255)                                                                   gTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGc                                                                                                                             >  1:597297/1‑102 (MQ=255)                                                                   gTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCAt                                                                             >  1:373544/1‑150 (MQ=255)                                                                     gcaCTTCAGTTTATCTGAAGAACTGCCGGGGTGGGGTACCGAACGGGCGTTTGAACATGCTGGCAAAGC‑CACTGGGGTTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCa                                                                           <  1:1255978/148‑1 (MQ=255)                                                                       aattaaGGTTATGTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGc                                                                         >  2:1730473/6‑150 (MQ=255)                                                                        cTTCAGGTTATCTGAATAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGcc                                                                        >  2:1459862/1‑150 (MQ=255)                                                                              gTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAGCATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGAGCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGccg                                                                  >  1:2308410/1‑149 (MQ=255)                                                                              gTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAg                                                                                                                                      >  1:1198456/1‑83 (MQ=255)                                                                              gTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAg                                                                                                                                      <  2:1198456/83‑1 (MQ=255)                                                                                 acctaaagagCTTACGGGGCGTGCTCCCGAACTGGCGCTTAAACCGGCTGG‑GAAGGTCTCTTGGTTTCTCTTAGCGAAAG‑CCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCctgct                                                               <  2:282150/145‑1 (MQ=255)                                                                                   cTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGGGGG‑CACTTGGGCTGTCATAGCCCAAG‑Gc                                                                                                                                   <  1:328159/80‑1 (MQ=255)                                                                                   cTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGGGGG‑CACTTGGGCTGTCATAGCCCAAG‑Gc                                                                                                                                   >  2:328159/1‑80 (MQ=255)                                                                                     gaagaaCTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTATGACAGCCCAAgt                                                                                                                                     >  2:626497/1‑76 (MQ=38)                                                                                     gaagaaCTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTATGACAGCCCAAgt                                                                                                                                     <  1:626497/77‑2 (MQ=38)                                                                                          ccgccgggggcgggcTCCCGCACGGGCGATTGAACATGTGGGGTAAGC‑CCCTGGGGCTCGGAGAGCCAAGG‑CCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACt                                                      <  1:1189649/143‑1 (MQ=255)                                                                                          actgactgGGCGTGGTACCGAACTGGCGCTTGAAAATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACt                                                      >  1:1066780/1‑150 (MQ=255)                                                                                                tgGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGa                                                >  1:2028264/1‑150 (MQ=255)                                                                                                  ggCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑Gccccc                                                                                                                               <  2:2259965/69‑1 (MQ=255)                                                                                                  ggCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑Gccccc                                                                                                                               >  1:2259965/1‑69 (MQ=255)                                                                                                     gTGGTACCGAACTGGCGCTTGAACAGGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTc                                           >  1:1873975/1‑150 (MQ=255)                                                                                                             gAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGt                                   >  2:209089/1‑150 (MQ=255)                                                                                                             gAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGt                                   >  1:223334/1‑150 (MQ=255)                                                                                                              aaCTGGCGCTTAACCATGCGGGCGAAGG‑CATTGGGGCTTCCATAGCCGAAG‑CCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGtt                                  <  1:1430170/150‑1 (MQ=255)                                                                                                                   gcgcTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCtt                             >  1:2062773/1‑150 (MQ=255)                                                                                                                     gcgtGAACGTGCTGGCAAGGG‑CACTGGGGCTGTAACAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGc                           <  2:1679686/147‑1 (MQ=255)                                                                                                                           aCATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGaaca                     >  2:1265192/1‑150 (MQ=255)                                                                                                                            cATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGaacaa                    >  1:1819049/1‑150 (MQ=255)                                                                                                                                 tGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAg                                                                                                    <  1:1537416/65‑1 (MQ=255)                                                                                                                                 tGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAg                                                                                                    >  2:1537416/1‑65 (MQ=255)                                                                                                                                 tGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCt               >  1:1699679/1‑150 (MQ=255)                                                                                                                                    cGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGa            <  1:209089/150‑1 (MQ=255)                                                                                                                                      aaGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGt                                            >  2:667811/1‑116 (MQ=255)                                                                                                                                      aaGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGt                                            <  1:667811/116‑1 (MQ=255)                                                                                                                                        ggggccTTGGGCTATGACAGCCCAAGTGCCTTCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATg          >  2:2387053/6‑150 (MQ=38)                                                                                                                                        gg‑CAATTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTAGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCCTGCCGGTTTCCTTGCGGAACAACCGATGGATGGt        >  2:2299556/1‑150 (MQ=255)                                                                                                                                            aCTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCtt     >  1:650811/1‑150 (MQ=255)                                                                                                                                            aCTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCtt     >  2:681052/1‑150 (MQ=255)                                                                                                                                             cTTGGGCTATGACAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCttt    >  1:2196903/1‑150 (MQ=255)                                                                                                                                              ttGGGCTATGACAGCCCAAGTGCCTTCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCttt    <  1:2387053/150‑1 (MQ=255)                                                                                                                                               tGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCTTTTc  <  2:1819049/150‑1 (MQ=255)                                                                                                                                                   cTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGaac                      >  2:287694/1‑126 (MQ=255)                                                                                                                                                   cTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGaac                      <  1:287694/126‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                 GCAAGAACAGGCAGGGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCTTTTC  >  NC_002947/2698403‑2698693

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑