Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,698,554 T→C 8.0% Y234C (TAT→TGT)  PP_2365 ← AraC family transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,698,5540TC 8.0% 180.7 / 3.2 68Y234C (TAT→TGT) PP_2365AraC family transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (38/23);  new base C (3/3);  total (41/27)
Fisher's exact test for biased strand distribution p-value = 6.70e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACAGGCAGGGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCTTTTCGTCCACC  >  NC_002947/2698407‑2698700
                                                                                                                                                    |                                                                                                                                                   
gAACAGGCAGGGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAg                                                                                                                                                   <  1:1070560/150‑1 (MQ=255)
 aaCAGGCAGGGCGAGCCGTCAGCGGAAAGCCAGTAAGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGc                                                                                                                                                  <  2:759935/150‑1 (MQ=255)
   caggcaggGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGccc                                                                                                                                                <  2:2046341/150‑1 (MQ=255)
     ggcaggGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCaa                                                                                                                                              <  1:2127534/150‑1 (MQ=255)
      gcaggGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAg                                                                                                                                             <  2:2123465/150‑1 (MQ=255)
      gcaggGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAg                                                                                                                                             >  2:2379871/1‑150 (MQ=255)
      gcaggGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAg                                                                                                                                             >  1:1679686/1‑150 (MQ=255)
                 cGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCcac                                                                                                                                 >  1:2070752/1‑150 (MQ=255)
                   tCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACAt                                                                                                                               >  2:1448238/1‑150 (MQ=255)
                      gCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCga                                                                                                                            >  2:1018589/1‑150 (MQ=255)
                      gCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGCGCGTGGTACCGAACTGGCGCTTGCACATGCTGGCGAACG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCga                                                                                                                            >  2:1018761/1‑150 (MQ=255)
                       ggaaCAGCCCGTATGGGGGGGGCCAGTGGGGGAAAATGGGTGGATTTCAGTTTATTTGAAAAATTGAGTGGGGGTGTTACCGAACTGGCGTTTGAACATGCTGGCGAAGG‑CATTGGGGCTTCAATAGCCCAGC‑GCCCCCGCCACATCgat                                                                                                                           <  2:1731809/147‑1 (MQ=255)
                        ggACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCgatg                                                                                                                          >  1:282150/1‑150 (MQ=255)
                         gACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCaa                                                                                                                                              <  1:1731601/130‑1 (MQ=255)
                         gACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCaa                                                                                                                                              >  2:1731601/1‑130 (MQ=255)
                            aGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGGGGACTTCAGGGTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATtc                                                                                                                      >  1:1862928/1‑150 (MQ=255)
                              cccGTATGCGGCGGGCCCGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATtcgc                                                                                                                    >  2:2021976/1‑150 (MQ=255)
                               ccGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATtcgct                                                                                                                   >  1:1008583/1‑150 (MQ=255)
                                     gcggcgGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCa                                                                                                             >  2:52255/1‑150 (MQ=255)
                                          gggCCAGTGCCGGCAAATGCGTGGACTTCGGTTTATCTGAAAAACTGACTGGGCGTGGTACCGAACTGGCGCTTAAACATGCTGGGGAAGG‑CACTTGGGCTGTCATAGCCAAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCa                                                                                                        <  1:2379871/150‑1 (MQ=255)
                                            gCCAGTGCCGGAAAATGCGTGGACTTCAGTTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAGGG‑CACTGGGGCTGTCATAGCCCAGG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAAt                                                                                                      <  1:1448238/150‑1 (MQ=255)
                                                      gCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGt                                                                                            >  2:1710922/1‑150 (MQ=255)
                                                          aTGCGTGGACTTCAGGTTATCGGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCGGGCGAAGG‑CACTTGGCCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCt                                                                                        <  1:582978/150‑1 (MQ=255)
                                                              gTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGc                                                                                                                                    <  2:597297/102‑1 (MQ=255)
                                                              gTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGc                                                                                                                                    >  1:597297/1‑102 (MQ=255)
                                                              gTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCAt                                                                                    >  1:373544/1‑150 (MQ=255)
                                                                gcaCTTCAGTTTATCTGAAGAACTGCCGGGGTGGGGTACCGAACGGGCGTTTGAACATGCTGGCAAAGC‑CACTGGGGTTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCa                                                                                  <  1:1255978/148‑1 (MQ=255)
                                                                  aattaaGGTTATGTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGc                                                                                >  2:1730473/6‑150 (MQ=255)
                                                                   cTTCAGGTTATCTGAATAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGcc                                                                               >  2:1459862/1‑150 (MQ=255)
                                                                         gTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAGCATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGAGCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGccg                                                                         >  1:2308410/1‑149 (MQ=255)
                                                                         gTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAg                                                                                                                                             <  2:1198456/83‑1 (MQ=255)
                                                                         gTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAg                                                                                                                                             >  1:1198456/1‑83 (MQ=255)
                                                                            acctaaagagCTTACGGGGCGTGCTCCCGAACTGGCGCTTAAACCGGCTGG‑GAAGGTCTCTTGGTTTCTCTTAGCGAAAG‑CCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCctgct                                                                      <  2:282150/145‑1 (MQ=255)
                                                                              cTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGGGGG‑CACTTGGGCTGTCATAGCCCAAG‑Gc                                                                                                                                          >  2:328159/1‑80 (MQ=255)
                                                                              cTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGGGGG‑CACTTGGGCTGTCATAGCCCAAG‑Gc                                                                                                                                          <  1:328159/80‑1 (MQ=255)
                                                                                gaagaaCTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTATGACAGCCCAAgt                                                                                                                                            >  2:626497/1‑76 (MQ=38)
                                                                                gaagaaCTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTATGACAGCCCAAgt                                                                                                                                            <  1:626497/77‑2 (MQ=38)
                                                                                     ccgccgggggcgggcTCCCGCACGGGCGATTGAACATGTGGGGTAAGC‑CCCTGGGGCTCGGAGAGCCAAGG‑CCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACt                                                             <  1:1189649/143‑1 (MQ=255)
                                                                                     actgactgGGCGTGGTACCGAACTGGCGCTTGAAAATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACt                                                             >  1:1066780/1‑150 (MQ=255)
                                                                                           tgGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGa                                                       >  1:2028264/1‑150 (MQ=255)
                                                                                             ggCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑Gccccc                                                                                                                                      <  2:2259965/69‑1 (MQ=255)
                                                                                             ggCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑Gccccc                                                                                                                                      >  1:2259965/1‑69 (MQ=255)
                                                                                                gTGGTACCGAACTGGCGCTTGAACAGGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTc                                                  >  1:1873975/1‑150 (MQ=255)
                                                                                                        gAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGt                                          >  1:223334/1‑150 (MQ=255)
                                                                                                        gAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGt                                          >  2:209089/1‑150 (MQ=255)
                                                                                                         aaCTGGCGCTTAACCATGCGGGCGAAGG‑CATTGGGGCTTCCATAGCCGAAG‑CCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGtt                                         <  1:1430170/150‑1 (MQ=255)
                                                                                                              gcgcTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCtt                                    >  1:2062773/1‑150 (MQ=255)
                                                                                                                gcgtGAACGTGCTGGCAAGGG‑CACTGGGGCTGTAACAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGc                                  <  2:1679686/147‑1 (MQ=255)
                                                                                                                      aCATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGaaca                            >  2:1265192/1‑150 (MQ=255)
                                                                                                                       cATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGaacaa                           >  1:1819049/1‑150 (MQ=255)
                                                                                                                            tGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAg                                                                                                           <  1:1537416/65‑1 (MQ=255)
                                                                                                                            tGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAg                                                                                                           >  2:1537416/1‑65 (MQ=255)
                                                                                                                            tGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCt                      >  1:1699679/1‑150 (MQ=255)
                                                                                                                               cGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGa                   <  1:209089/150‑1 (MQ=255)
                                                                                                                                 aaGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGt                                                   >  2:667811/1‑116 (MQ=255)
                                                                                                                                 aaGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGt                                                   <  1:667811/116‑1 (MQ=255)
                                                                                                                                   ggggccTTGGGCTATGACAGCCCAAGTGCCTTCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATg                 >  2:2387053/6‑150 (MQ=38)
                                                                                                                                   gg‑CAATTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTAGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCCTGCCGGTTTCCTTGCGGAACAACCGATGGATGGt               >  2:2299556/1‑150 (MQ=255)
                                                                                                                                       aCTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCtt            >  2:681052/1‑150 (MQ=255)
                                                                                                                                       aCTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCtt            >  1:650811/1‑150 (MQ=255)
                                                                                                                                        cTTGGGCTATGACAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCttt           >  1:2196903/1‑150 (MQ=255)
                                                                                                                                         ttGGGCTATGACAGCCCAAGTGCCTTCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCttt           <  1:2387053/150‑1 (MQ=255)
                                                                                                                                          tGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCTTTTc         <  2:1819049/150‑1 (MQ=255)
                                                                                                                                              cTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGaac                             >  2:287694/1‑126 (MQ=255)
                                                                                                                                              cTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGaac                             <  1:287694/126‑1 (MQ=255)
                                                                                                                                                 tCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCtt            <  1:1579980/140‑1 (MQ=255)
                                                                                                                                                 tCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCtt            >  2:1579980/1‑140 (MQ=255)
                                                                                                                                                 tCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCTTTTCGTCCAcc  >  1:1226022/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
GAACAGGCAGGGCGAGCCGTCAGCGGACAGCCCGTATGCGGCGGGCCAGTGCCGGCAAATGCGTGGACTTCAGGTTATCTGAAGAACTGACTGGGCGTGGTACCGAACTGGCGCTTGAACATGCTGGCGAAGG‑CACTTGGGCTGTCATAGCCCAAG‑GCCCCCGCCACATCGATGATTCGCTCGCCCAAGGCAATCCGCTCCAGTGCCTGCATCAGCCGCGCCTGCTGGCGCCACTGGCCGAAGGTCATGCCGGTTTCCTTGCGGAACAACCGCTGGATGGTCTTTTCGTCCACC  >  NC_002947/2698407‑2698700

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑