Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,810,636	C→A	9.6%	P334T (CCG→ACG)	thrS →	threonine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,810,636	0	C	A	9.6%	215.2 / 10.3	74	P334T (CCG→ACG)	thrS	threonine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (35/31);  new base A (4/3);  total (39/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.48e-01

GCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATGC  >  NC_002947/2810488‑2810784                                                                                                                                                     |                                                                                                                                                      gcgcGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGccc                                                                                                                                                      >  2:907139/1‑150 (MQ=255)     gAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGc                                                                                                                                                  >  2:1427782/1‑150 (MQ=255)       aaaCGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCa                                                                                                                                                <  1:547563/150‑1 (MQ=255)           ggCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTg                                                                                                                                            <  2:1061155/150‑1 (MQ=255)              tACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAg                                                                                                                                         <  2:896519/150‑1 (MQ=255)              tACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAg                                                                                                                                         <  1:52371/150‑1 (MQ=255)                ccAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCATGTGCAGgt                                                                                                                                       >  1:2113132/1‑150 (MQ=255)                ccAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGgt                                                                                                                                       >  2:1915186/1‑150 (MQ=255)                ccAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGgt                                                                                                                                       <  2:1735758/150‑1 (MQ=255)                 cAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGgtg                                                                                                                                      <  2:15442/150‑1 (MQ=255)                 cAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGgtg                                                                                                                                      >  1:2017875/1‑150 (MQ=255)                       aTCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAAc                                                                                                                                <  2:1252038/150‑1 (MQ=255)                          aaGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAg                                                                                                                             <  1:907139/150‑1 (MQ=255)                          aaGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCACGTGGCACGGGCAgttc                                                                                                                                      <  1:425212/141‑4 (MQ=255)                          aaGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCACGTGGCACGGGCAgttc                                                                                                                                      >  2:425212/1‑138 (MQ=255)                            gACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAggg                                                                                                                           <  1:40384/150‑1 (MQ=255)                            gACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAggg                                                                                                                           >  2:1116391/1‑150 (MQ=255)                               cccGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCt                                                                                                                        >  2:212095/1‑150 (MQ=255)                                ccGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTg                                                                                                                       >  1:1247612/1‑150 (MQ=255)                                 cGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGa                                                                                                                      <  2:2093212/150‑1 (MQ=255)                                   cAGGTTGTCGACCGCATCCTGTGGGAGCGTTTCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGACCGTGCCACGTGAAGGGGTTGAACCAGGGCCTGaaa                                                                                                                    >  1:435063/1‑150 (MQ=255)                                    aGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGaaaa                                                                                                                   <  2:716227/150‑1 (MQ=255)                                    aGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGAAACTGGTCCAACTATGACGAAAACCTGTTCAACACTTCGTCGGAAAGCCGTGACTACGCGGTAAACCCGATGAACTGCCCATGCCACGTGCAGGTGTTCAACCAGGGCCTGaaaa                                                                                                                   <  2:1108787/150‑1 (MQ=255)                                     ggTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAg                                                                                                                  >  2:1552694/1‑150 (MQ=255)                                      gTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGc                                                                                                                 >  1:2321914/1‑150 (MQ=255)                                      gTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGc                                                                                                                 >  1:1591856/1‑150 (MQ=255)                                        tGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTa                                                                                                               >  2:317072/1‑150 (MQ=255)                                         gTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGt                                                                                                                                             >  2:627082/1‑119 (MQ=255)                                         gTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGt                                                                                                                                             <  1:627082/119‑1 (MQ=255)                                         gTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTAc                                                                                                              <  2:1247612/150‑1 (MQ=255)                                          tCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTAcc                                                                                                             >  2:598694/1‑150 (MQ=255)                                                     cTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCg                                                                                                  >  2:1534620/1‑150 (MQ=255)                                                        tgGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTg                                                                                               <  2:1980908/150‑1 (MQ=255)                                                         gggAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGc                                                                                              <  1:212095/150‑1 (MQ=255)                                                                ttCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGCAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGc                                                                                       >  1:440134/1‑150 (MQ=255)                                                                   cGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGa                                                                                    <  1:1116391/150‑1 (MQ=255)                                                                    ggCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCATGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAg                                                                                   <  2:2113132/150‑1 (MQ=255)                                                                       cACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCACGTgg                                                                                                                                                  >  1:838635/1‑83 (MQ=255)                                                                       cACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCACGTgg                                                                                                                                                  <  2:838635/84‑2 (MQ=255)                                                                         cTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCgg                                                                              <  1:1427782/150‑1 (MQ=255)                                                                             tCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTcagatcg                                                                          >  2:1750301/1‑144 (MQ=255)                                                                                ccgatctccGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGtgcctgc                                                                       <  1:442509/143‑1 (MQ=255)                                                                                aaCTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGtgcctgc                                                                       >  1:96103/1‑150 (MQ=255)                                                                                aaCTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGtgcctgc                                                                       <  1:1534620/150‑1 (MQ=255)                                                                                   tATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGtctc                                                                                         <  1:1887435/129‑1 (MQ=255)                                                                                   tATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGtctc                                                                                         >  2:1887435/1‑129 (MQ=255)                                                                                        cGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTaa                                                               >  1:993732/1‑150 (MQ=255)                                                                                        cGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGACACTTGCAGGTGTTCAACCAGAGCCTGACAAGCTACCGCGCCCAGCCGCTACGTCGCTCCGAGGTCCGTGCTGGCCagatcgg                                                               >  2:442509/1‑144 (MQ=255)                                                                                         gAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAAc                                                              >  1:1382072/1‑150 (MQ=255)                                                                                          aaaaCATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGATACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACg                                                             >  2:1470849/1‑150 (MQ=255)                                                                                              cATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGcc                                                         >  1:593612/1‑150 (MQ=255)                                                                                               aTGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTAc                                                                                                              <  1:1566439/96‑1 (MQ=255)                                                                                               aTGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTAc                                                                                                              >  2:1566439/1‑96 (MQ=255)                                                                                                tGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGt                                                       >  1:1943844/1‑150 (MQ=255)                                                                                                      ccacTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGgcg                                                 >  2:251262/1‑150 (MQ=255)                                                                                                          ttCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTc                                                                                <  2:2387704/115‑1 (MQ=255)                                                                                                          ttCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTc                                                                                >  1:2387704/1‑115 (MQ=255)                                                                                                                 gAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑Gc                                     >  2:560309/1‑150 (MQ=255)                                                                                                                 gAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑Gc                                     >  1:159919/1‑150 (MQ=255)                                                                                                                        gTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATgc                              >  1:831387/1‑150 (MQ=255)                                                                                                                        gTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGAGCCCTGCACG‑GCATCATgc                              >  2:1582980/1‑150 (MQ=255)                                                                                                                               cgcgGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACGCGCATGATGc‑cgtgca                       <  2:1184374/150‑2 (MQ=255)                                                                                                                                gcgGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCgcgtgcgt                      >  2:779314/1‑150 (MQ=255)                                                                                                                                  ggTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACGCGCATGATGc‑cgtgcaggg                    <  1:1061201/150‑5 (MQ=255)                                                                                                                                     aaaaCCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCtt                 <  2:440134/150‑1 (MQ=255)                                                                                                                                      aaaCCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCg                                                    <  2:112660/115‑1 (MQ=255)                                                                                                                                      aaaCCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCg                                                    >  1:112660/1‑115 (MQ=255)                                                                                                                                         ccGATGAACTCACCGTGCCACGAGCAGGTGTTCACCCAGCGCCTCAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACTAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTa             <  1:1552694/150‑1 (MQ=255)                                                                                                                                          cGATGAACCGCGCGTTCCCCGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTAc            <  2:1943844/150‑1 (MQ=255)                                                                                                                                            aTGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAg          <  1:598694/150‑1 (MQ=255)                                                                                                                                              gAACTGCCCGTGCCAGGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGa        <  2:2137304/150‑1 (MQ=255)                                                                                                                                                aCTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTAAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACg      >  1:1647925/1‑150 (MQ=255)                                                                                                                                                 cTGCACGTGGCACGGGCAGTTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCgcgtgcgt                      >  2:1986676/1‑133 (MQ=255)                                                                                                                                                 cTGCACGTGGCACGGGCAGTTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCgcgtgcgt                      <  1:1986676/133‑1 (MQ=255)                                                                                                                                                 cTGCACGTGGCACGGGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGa     >  1:1379118/1‑150 (MQ=255)                                                                                                                                                    cccGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATgc  >  1:1205825/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                      GCGCGAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATGC  >  NC_002947/2810488‑2810784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑