Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,810,641	C→G	7.0%	C335W (TGC→TGG)	thrS →	threonine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,810,641	0	C	G	7.0%	220.1 / 7.4	72	C335W (TGC→TGG)	thrS	threonine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base C (34/32);  new base G (3/2);  total (38/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.19e-01

GAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATGCGCACAT  >  NC_002947/2810492‑2810790                                                                                                                                                      |                                                                                                                                                       gAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGc                                                                                                                                                        >  2:1427782/1‑150 (MQ=255)   aaaCGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCa                                                                                                                                                      <  1:547563/150‑1 (MQ=255)       ggCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTg                                                                                                                                                  <  2:1061155/150‑1 (MQ=255)          tACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAg                                                                                                                                               <  2:896519/150‑1 (MQ=255)          tACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAg                                                                                                                                               <  1:52371/150‑1 (MQ=255)            ccAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCATGTGCAGgt                                                                                                                                             >  1:2113132/1‑150 (MQ=255)            ccAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGgt                                                                                                                                             >  2:1915186/1‑150 (MQ=255)            ccAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGgt                                                                                                                                             <  2:1735758/150‑1 (MQ=255)             cAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGgtg                                                                                                                                            >  1:2017875/1‑150 (MQ=255)             cAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGgtg                                                                                                                                            <  2:15442/150‑1 (MQ=255)                   aTCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAAc                                                                                                                                      <  2:1252038/150‑1 (MQ=255)                      aaGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAg                                                                                                                                   <  1:907139/150‑1 (MQ=255)                      aaGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCACGTGGCACGGGCAgttc                                                                                                                                            >  2:425212/1‑138 (MQ=255)                      aaGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCACGTGGCACGGGCAgttc                                                                                                                                            <  1:425212/141‑4 (MQ=255)                        gACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAggg                                                                                                                                 >  2:1116391/1‑150 (MQ=255)                        gACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAggg                                                                                                                                 <  1:40384/150‑1 (MQ=255)                           cccGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCt                                                                                                                              >  2:212095/1‑150 (MQ=255)                            ccGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTg                                                                                                                             >  1:1247612/1‑150 (MQ=255)                             cGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGa                                                                                                                            <  2:2093212/150‑1 (MQ=255)                               cAGGTTGTCGACCGCATCCTGTGGGAGCGTTTCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGACCGTGCCACGTGAAGGGGTTGAACCAGGGCCTGaaa                                                                                                                          >  1:435063/1‑150 (MQ=255)                                aGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGaaaa                                                                                                                         <  2:716227/150‑1 (MQ=255)                                aGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGAAACTGGTCCAACTATGACGAAAACCTGTTCAACACTTCGTCGGAAAGCCGTGACTACGCGGTAAACCCGATGAACTGCCCATGCCACGTGCAGGTGTTCAACCAGGGCCTGaaaa                                                                                                                         <  2:1108787/150‑1 (MQ=255)                                 ggTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAg                                                                                                                        >  2:1552694/1‑150 (MQ=255)                                  gTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGc                                                                                                                       >  1:2321914/1‑150 (MQ=255)                                  gTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGc                                                                                                                       >  1:1591856/1‑150 (MQ=255)                                    tGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTa                                                                                                                     >  2:317072/1‑150 (MQ=255)                                     gTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGt                                                                                                                                                   >  2:627082/1‑119 (MQ=255)                                     gTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGt                                                                                                                                                   <  1:627082/119‑1 (MQ=255)                                     gTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTAc                                                                                                                    <  2:1247612/150‑1 (MQ=255)                                      tCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTAcc                                                                                                                   >  2:598694/1‑150 (MQ=255)                                                 cTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCg                                                                                                        >  2:1534620/1‑150 (MQ=255)                                                    tgGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTg                                                                                                     <  2:1980908/150‑1 (MQ=255)                                                     gggAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGc                                                                                                    <  1:212095/150‑1 (MQ=255)                                                            ttCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGCAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGc                                                                                             >  1:440134/1‑150 (MQ=255)                                                               cGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGa                                                                                          <  1:1116391/150‑1 (MQ=255)                                                                ggCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCATGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAg                                                                                         <  2:2113132/150‑1 (MQ=255)                                                                     cTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCgg                                                                                    <  1:1427782/150‑1 (MQ=255)                                                                         tCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTcagatcg                                                                                >  2:1750301/1‑144 (MQ=255)                                                                            ccgatctccGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGtgcctgc                                                                             <  1:442509/143‑1 (MQ=255)                                                                            aaCTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGtgcctgc                                                                             >  1:96103/1‑150 (MQ=255)                                                                            aaCTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGtgcctgc                                                                             <  1:1534620/150‑1 (MQ=255)                                                                               tATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGtctc                                                                                               <  1:1887435/129‑1 (MQ=255)                                                                               tATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGtctc                                                                                               >  2:1887435/1‑129 (MQ=255)                                                                                    cGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTaa                                                                     >  1:993732/1‑150 (MQ=255)                                                                                    cGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGACACTTGCAGGTGTTCAACCAGAGCCTGACAAGCTACCGCGCCCAGCCGCTACGTCGCTCCGAGGTCCGTGCTGGCCagatcgg                                                                     >  2:442509/1‑144 (MQ=255)                                                                                     gAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAAc                                                                    >  1:1382072/1‑150 (MQ=255)                                                                                      aaaaCATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGATACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACg                                                                   >  2:1470849/1‑150 (MQ=255)                                                                                          cATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGcc                                                               >  1:593612/1‑150 (MQ=255)                                                                                           aTGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTAc                                                                                                                    >  2:1566439/1‑96 (MQ=255)                                                                                           aTGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTAc                                                                                                                    <  1:1566439/96‑1 (MQ=255)                                                                                            tGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGt                                                             >  1:1943844/1‑150 (MQ=255)                                                                                                  ccacTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGgcg                                                       >  2:251262/1‑150 (MQ=255)                                                                                                      ttCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTc                                                                                      >  1:2387704/1‑115 (MQ=255)                                                                                                      ttCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTc                                                                                      <  2:2387704/115‑1 (MQ=255)                                                                                                             gAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑Gc                                           >  2:560309/1‑150 (MQ=255)                                                                                                             gAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑Gc                                           >  1:159919/1‑150 (MQ=255)                                                                                                                    gTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATgc                                    >  1:831387/1‑150 (MQ=255)                                                                                                                    gTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGAGCCCTGCACG‑GCATCATgc                                    >  2:1582980/1‑150 (MQ=255)                                                                                                                           cgcgGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACGCGCATGATGc‑cgtgca                             <  2:1184374/150‑2 (MQ=255)                                                                                                                            gcgGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCgcgtgcgt                            >  2:779314/1‑150 (MQ=255)                                                                                                                              ggTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACGCGCATGATGc‑cgtgcaggg                          <  1:1061201/150‑5 (MQ=255)                                                                                                                                 aaaaCCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCtt                       <  2:440134/150‑1 (MQ=255)                                                                                                                                  aaaCCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCg                                                          >  1:112660/1‑115 (MQ=255)                                                                                                                                  aaaCCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCg                                                          <  2:112660/115‑1 (MQ=255)                                                                                                                                     ccGATGAACTCACCGTGCCACGAGCAGGTGTTCACCCAGCGCCTCAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACTAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTa                   <  1:1552694/150‑1 (MQ=255)                                                                                                                                      cGATGAACCGCGCGTTCCCCGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTAc                  <  2:1943844/150‑1 (MQ=255)                                                                                                                                        aTGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAg                <  1:598694/150‑1 (MQ=255)                                                                                                                                          gAACTGCCCGTGCCAGGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGa              <  2:2137304/150‑1 (MQ=255)                                                                                                                                            aCTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTAAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACg            >  1:1647925/1‑150 (MQ=255)                                                                                                                                             cTGCACGTGGCACGGGCAGTTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCgcgtgcgt                            >  2:1986676/1‑133 (MQ=255)                                                                                                                                             cTGCACGTGGCACGGGCAGTTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCgcgtgcgt                            <  1:1986676/133‑1 (MQ=255)                                                                                                                                             cTGCACGTGGCACGGGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGa           >  1:1379118/1‑150 (MQ=255)                                                                                                                                                cccGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATgc        >  1:1205825/1‑150 (MQ=255)                                                                                                                                                      ccACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGa              <  2:286580/138‑1 (MQ=255)                                                                                                                                                      ccACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGa              >  1:286580/1‑138 (MQ=255)                                                                                                                                                      ccACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATGCGCGCAt  >  2:2336054/1‑150 (MQ=255)                                                                                                                                                      |                                                                                                                                                       GAAAACGGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATGCGCACAT  >  NC_002947/2810492‑2810790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑