Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	2,810,646	T→G	6.8%	V337G (GTG→GGG)	thrS →	threonine‑‑tRNA ligase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	2,810,646	0	T	G	6.8%	214.2 / 6.9	75	V337G (GTG→GGG)	thrS	threonine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base T (36/33);  new base G (3/2);  total (39/36)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.68e-01

GGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATGCGCACATTTTCT  >  NC_002947/2810498‑2810795                                                                                                                                                     |                                                                                                                                                       ggCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTg                                                                                                                                                       <  2:1061155/150‑1 (MQ=255)    tACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAg                                                                                                                                                    <  2:896519/150‑1 (MQ=255)    tACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAg                                                                                                                                                    <  1:52371/150‑1 (MQ=255)      ccAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCATGTGCAGgt                                                                                                                                                  >  1:2113132/1‑150 (MQ=255)      ccAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGgt                                                                                                                                                  >  2:1915186/1‑150 (MQ=255)      ccAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGgt                                                                                                                                                  <  2:1735758/150‑1 (MQ=255)       cAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGgtg                                                                                                                                                 >  1:2017875/1‑150 (MQ=255)       cAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGgtg                                                                                                                                                 <  2:15442/150‑1 (MQ=255)             aTCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAAc                                                                                                                                           <  2:1252038/150‑1 (MQ=255)                aaGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAg                                                                                                                                        <  1:907139/150‑1 (MQ=255)                aaGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCACGTGGCACGGGCAgttc                                                                                                                                                 <  1:425212/141‑4 (MQ=255)                aaGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCACGTGGCACGGGCAgttc                                                                                                                                                 >  2:425212/1‑138 (MQ=255)                  gACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAggg                                                                                                                                      >  2:1116391/1‑150 (MQ=255)                  gACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAggg                                                                                                                                      <  1:40384/150‑1 (MQ=255)                     cccGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCt                                                                                                                                   >  2:212095/1‑150 (MQ=255)                      ccGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTg                                                                                                                                  >  1:1247612/1‑150 (MQ=255)                       cGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGa                                                                                                                                 <  2:2093212/150‑1 (MQ=255)                         cAGGTTGTCGACCGCATCCTGTGGGAGCGTTTCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGACCGTGCCACGTGAAGGGGTTGAACCAGGGCCTGaaa                                                                                                                               >  1:435063/1‑150 (MQ=255)                          aGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGaaaa                                                                                                                              <  2:716227/150‑1 (MQ=255)                          aGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGAAACTGGTCCAACTATGACGAAAACCTGTTCAACACTTCGTCGGAAAGCCGTGACTACGCGGTAAACCCGATGAACTGCCCATGCCACGTGCAGGTGTTCAACCAGGGCCTGaaaa                                                                                                                              <  2:1108787/150‑1 (MQ=255)                           ggTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAg                                                                                                                             >  2:1552694/1‑150 (MQ=255)                            gTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGc                                                                                                                            >  1:2321914/1‑150 (MQ=255)                            gTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGc                                                                                                                            >  1:1591856/1‑150 (MQ=255)                              tGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTa                                                                                                                          >  2:317072/1‑150 (MQ=255)                               gTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGt                                                                                                                                                        >  2:627082/1‑119 (MQ=255)                               gTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGt                                                                                                                                                        <  1:627082/119‑1 (MQ=255)                               gTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTAc                                                                                                                         <  2:1247612/150‑1 (MQ=255)                                tCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTAcc                                                                                                                        >  2:598694/1‑150 (MQ=255)                                           cTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCg                                                                                                             >  2:1534620/1‑150 (MQ=255)                                              tgGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTg                                                                                                          <  2:1980908/150‑1 (MQ=255)                                               gggAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGc                                                                                                         <  1:212095/150‑1 (MQ=255)                                                      ttCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGCAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGc                                                                                                  >  1:440134/1‑150 (MQ=255)                                                         cGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGa                                                                                               <  1:1116391/150‑1 (MQ=255)                                                          ggCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCATGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAg                                                                                              <  2:2113132/150‑1 (MQ=255)                                                               cTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCgg                                                                                         <  1:1427782/150‑1 (MQ=255)                                                                   tCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTcagatcg                                                                                     >  2:1750301/1‑144 (MQ=255)                                                                      ccgatctccGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGtgcctgc                                                                                  <  1:442509/143‑1 (MQ=255)                                                                      aaCTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGtgcctgc                                                                                  <  1:1534620/150‑1 (MQ=255)                                                                      aaCTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGtgcctgc                                                                                  >  1:96103/1‑150 (MQ=255)                                                                         tATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGtctc                                                                                                    >  2:1887435/1‑129 (MQ=255)                                                                         tATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGtctc                                                                                                    <  1:1887435/129‑1 (MQ=255)                                                                              cGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTaa                                                                          >  1:993732/1‑150 (MQ=255)                                                                              cGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGACACTTGCAGGTGTTCAACCAGAGCCTGACAAGCTACCGCGCCCAGCCGCTACGTCGCTCCGAGGTCCGTGCTGGCCagatcgg                                                                          >  2:442509/1‑144 (MQ=255)                                                                               gAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAAc                                                                         >  1:1382072/1‑150 (MQ=255)                                                                                aaaaCATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGATACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACg                                                                        >  2:1470849/1‑150 (MQ=255)                                                                                    cATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGcc                                                                    >  1:593612/1‑150 (MQ=255)                                                                                     aTGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTAc                                                                                                                         <  1:1566439/96‑1 (MQ=255)                                                                                     aTGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTAc                                                                                                                         >  2:1566439/1‑96 (MQ=255)                                                                                      tGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGt                                                                  >  1:1943844/1‑150 (MQ=255)                                                                                            ccacTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGgcg                                                            >  2:251262/1‑150 (MQ=255)                                                                                                ttCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTc                                                                                           <  2:2387704/115‑1 (MQ=255)                                                                                                ttCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTc                                                                                           >  1:2387704/1‑115 (MQ=255)                                                                                                       gAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑Gc                                                >  2:560309/1‑150 (MQ=255)                                                                                                       gAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑Gc                                                >  1:159919/1‑150 (MQ=255)                                                                                                              gTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATgc                                         >  1:831387/1‑150 (MQ=255)                                                                                                              gTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGAGCCCTGCACG‑GCATCATgc                                         >  2:1582980/1‑150 (MQ=255)                                                                                                                     cgcgGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACGCGCATGATGc‑cgtgca                                  <  2:1184374/150‑2 (MQ=255)                                                                                                                      gcgGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCgcgtgcgt                                 >  2:779314/1‑150 (MQ=255)                                                                                                                        ggTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACGCGCATGATGc‑cgtgcaggg                               <  1:1061201/150‑5 (MQ=255)                                                                                                                           aaaaCCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCtt                            <  2:440134/150‑1 (MQ=255)                                                                                                                            aaaCCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCg                                                               >  1:112660/1‑115 (MQ=255)                                                                                                                            aaaCCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCg                                                               <  2:112660/115‑1 (MQ=255)                                                                                                                               ccGATGAACTCACCGTGCCACGAGCAGGTGTTCACCCAGCGCCTCAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACTAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTa                        <  1:1552694/150‑1 (MQ=255)                                                                                                                                cGATGAACCGCGCGTTCCCCGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTAc                       <  2:1943844/150‑1 (MQ=255)                                                                                                                                  aTGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAg                     <  1:598694/150‑1 (MQ=255)                                                                                                                                    gAACTGCCCGTGCCAGGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGa                   <  2:2137304/150‑1 (MQ=255)                                                                                                                                      aCTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTAAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACg                 >  1:1647925/1‑150 (MQ=255)                                                                                                                                       cTGCACGTGGCACGGGCAGTTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCgcgtgcgt                                 <  1:1986676/133‑1 (MQ=255)                                                                                                                                       cTGCACGTGGCACGGGCAGTTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCgcgtgcgt                                 >  2:1986676/1‑133 (MQ=255)                                                                                                                                       cTGCACGTGGCACGGGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGa                >  1:1379118/1‑150 (MQ=255)                                                                                                                                          cccGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATgc             >  1:1205825/1‑150 (MQ=255)                                                                                                                                                ccACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGa                   <  2:286580/138‑1 (MQ=255)                                                                                                                                                ccACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGa                   >  1:286580/1‑138 (MQ=255)                                                                                                                                                ccACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATGCGCGCAt       >  2:2336054/1‑150 (MQ=255)                                                                                                                                                 cACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATGCGCTCAtt      <  1:779314/150‑1 (MQ=255)                                                                                                                                                   cGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATGCGCACAtttt    <  1:317072/150‑1 (MQ=255)                                                                                                                                                    gTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATGCGCACATTTTc   <  2:1205825/150‑1 (MQ=255)                                                                                                                                                     tGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGATCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATGCGCACATTTTCt  <  2:2017875/150‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                       GGCTACCAGGAAATCAAGACCCCGCAGGTTGTCGACCGCATCCTGTGGGAGCGTTCCGGCCACTGGTCCAACTATGCCGAAAACATGTTCACCACTTCGTCGGAAAGCCGTGACTACGCGGTAAAACCGATGAACTGCCCGTGCCACGTGCAGGTGTTCAACCAGGGCCTGAAAAGCTACCGCGACCTGCCGCTGCGTCTCGCCGAGTTCGGTGCCTGCCACCGTAACGAGCCGTCCGGCGCCCTGCACG‑GCATCATGCGCGTGCGTGGCTTCGTACAGGACGATGCGCACATTTTCT  >  NC_002947/2810498‑2810795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑