Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 2,894,016 G→C 6.8% A321A (GCG→GCC)  PP_2548 → sugar MFS transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029472,894,0160GC 6.8% 284.0 / 5.7 92A321A (GCG→GCC) PP_2548sugar MFS transporter
Reads supporting (aligned to +/- strand):  ref base G (39/46);  new base C (3/4);  total (42/50)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCATCCCGACTGCTTTCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGCAGCAGGTTTTGTC  >  NC_002947/2893871‑2894163
                                                                                                                                                 |                                                                                                                                                   
tcatcCCGACTGCTTTCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCa                                                                                                                                                 <  1:413381/150‑1 (MQ=255)
     ccGACTGCTTTCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCCGCCATGAac                                                                                                                                            <  1:2024720/150‑1 (MQ=255)
      cGACTGCTTTCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAaca                                                                                                                                           >  2:1168996/1‑150 (MQ=255)
      cGACTGCTTTCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACACTGTCGGCGTTCATGGCGGCCATGAaca                                                                                                                                           <  1:328581/150‑1 (MQ=255)
       gACTGCTTTCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGTTCAGCGTCGTGCTGCGGGCCGTGACATTGGCGGCGTTCATGGCGGCCATGAacac                                                                                                                                          <  1:910200/150‑1 (MQ=255)
          tGCTTTCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCgg                                                                                                                                       >  2:1741379/1‑150 (MQ=255)
            cTTTCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCa                                                                                                                                     <  1:403262/150‑1 (MQ=255)
             tttCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACcagcag                                                                                                                                    >  1:78790/1‑150 (MQ=255)
             tttCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCgg                                                                                                                                       <  1:2327759/147‑1 (MQ=255)
             tttCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCgg                                                                                                                                       >  2:2327759/1‑147 (MQ=255)
             tttCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAg                                                                                                                                    >  1:271275/1‑150 (MQ=255)
             tttCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGAAAAACAGTTAGGAGCCCGGCACCCGTCAGCCTTCGTTGTCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAg                                                                                                                                    >  1:294739/1‑150 (MQ=255)
             tttCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCCAACCTCGGACATACTTTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAg                                                                                                                                    >  1:1253951/1‑150 (MQ=255)
              ttCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGTGTTCATGGCCGGCATGAACGCCgccaat                                                                                                                                   <  1:2000801/150‑6 (MQ=255)
                 gAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCAt                                                                                                                                                >  1:915145/1‑134 (MQ=255)
                 gAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCAt                                                                                                                                                <  2:915145/134‑1 (MQ=255)
                   aCTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGcacgct                                                                                                                              <  2:587921/150‑6 (MQ=255)
                    cTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGtgctg                                                                                                                             >  2:1056522/1‑150 (MQ=255)
                     tGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGtgctgc                                                                                                                            >  1:519804/1‑150 (MQ=255)
                       tccGGGCTGCTTTGCCCCAGCAGGAGCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATCGCGGCCATGAACACCGGCAGCGTGctgctg                                                                                                                          <  2:271275/149‑1 (MQ=255)
                            gctgctTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCtggtggt                                                                                                                     <  2:1682446/150‑1 (MQ=255)
                             ctgctTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCtggtggtg                                                                                                                    <  1:702731/150‑1 (MQ=255)
                              tgctTGGCCCGATCATGATCGGCCTGGCCTCGGACAACCTCTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCAGCAGCACGCTGCcggtgttca                                                                                                                   <  1:23653/150‑5 (MQ=255)
                              tgctTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCAGCAGCACGCTGCcggtgttca                                                                                                                   <  1:2212834/150‑5 (MQ=255)
                              tgctTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCGGGCGCTGCGGACCGTGACAGTGGCGGGGGTCGTGGCGTCGGTGAACACCGGCAGCGGGCGGCtgacggtgg                                                                                                                   >  2:2308553/1‑150 (MQ=255)
                               gtttGGCGCGATCATGATCGGCCTGGCCTGGGACAAAGGGTTTGGGGGCCGGCGCATGCCAGCCTGGGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCAGCAGCACGCTGCcggtgttcat                                                                                                                  <  2:966670/148‑6 (MQ=255)
                                 ttGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCt                                                                                                                <  2:643850/150‑1 (MQ=255)
                                 ttGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCt                                                                                                                >  1:483089/1‑150 (MQ=255)
                                 ttGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCt                                                                                                                >  2:970128/1‑150 (MQ=255)
                                 ttGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGAAATTGCCGGTGTTCATGGCCGCCATGAACGCCgc                                                                                                                                       >  2:632365/1‑126 (MQ=39)
                                 ttGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGAAATTGCCGGTGTTCATGGCCGCCATGAACGCCgc                                                                                                                                       <  1:632365/127‑2 (MQ=38)
                                    gCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGt                                                                                                                                 <  2:570147/130‑1 (MQ=255)
                                    gCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGt                                                                                                                                 >  1:570147/1‑130 (MQ=255)
                                    gCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGc                                                                                                             >  1:193308/1‑150 (MQ=255)
                                      ccGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTg                                                                                                           >  2:92958/1‑150 (MQ=255)
                                      ccGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTACTGACCGTGACATTGGCGGCTTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTg                                                                                                           >  1:2091919/1‑150 (MQ=255)
                                             tGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCa                                                                                                    <  2:2143507/150‑1 (MQ=255)
                                                      tggcctCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCtt                                                                                                               >  2:1907038/1‑130 (MQ=255)
                                                      tggcctCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCtt                                                                                                               <  1:1907038/130‑1 (MQ=255)
                                                        gcctCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGAcc                                                                                         <  2:1546063/150‑1 (MQ=255)
                                                          ctCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCtggtgt                                                                                                                      >  1:2381874/1‑118 (MQ=255)
                                                          ctCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCtggtgt                                                                                                                      <  2:2381874/119‑2 (MQ=255)
                                                           tCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTc                                                                                                        >  1:1535177/1‑132 (MQ=255)
                                                           tCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTc                                                                                                        <  2:1535177/132‑1 (MQ=255)
                                                           tCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGAc                                                                                          >  2:6004/1‑146 (MQ=255)
                                                           tCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGAc                                                                                          <  1:6004/146‑1 (MQ=255)
                                                           tCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTg                                                                                      <  1:1429920/150‑1 (MQ=255)
                                                               aCAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACg                                                                                  >  2:1485055/1‑150 (MQ=255)
                                                                cAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTTCTGCTGGTGGTGGCCTTGATGTTCGTCATGGGTCTGACCCTGTACgg                                                                                 >  1:976545/1‑150 (MQ=255)
                                                                 aaaCTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTTCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACggg                                                                                >  2:226733/1‑150 (MQ=255)
                                                                 aaaCTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACggg                                                                                >  1:742974/1‑150 (MQ=255)
                                                                 aaaCTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACggg                                                                                >  2:101674/1‑150 (MQ=255)
                                                                         tGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTc                                                                        >  2:1880610/1‑150 (MQ=255)
                                                                         tGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTc                                                                        <  1:1724746/150‑1 (MQ=255)
                                                                           gcgcCCGGCGCATGCCAGCCTGCGTGCTCCGCCGGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCga                                                                      <  2:193308/150‑1 (MQ=255)
                                                                            ggcCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCgat                                                                     <  2:1457526/149‑1 (MQ=255)
                                                                            cgcCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCgat                                                                     >  1:1811161/1‑150 (MQ=255)
                                                                            cgcCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCgat                                                                     <  1:970128/150‑1 (MQ=255)
                                                                            cgcCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCgat                                                                     <  2:958226/150‑1 (MQ=255)
                                                                             gcCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATgg                                                                                                 >  2:1390940/1‑121 (MQ=255)
                                                                             gcCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATgg                                                                                                 <  1:1390940/121‑1 (MQ=255)
                                                                               ccGACGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGCCATTGGCGGCGTTCATGGCGGCCATGAAAACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCgatgat                                                                  >  1:660914/1‑150 (MQ=255)
                                                                                   cgcATGCCAGCCGGCGTGATCAGCCTGGTGCTGCTGACCTGGACATTGGCGGCGTACATGGCGGCCATGAACACCGGCAGCGGGCTGCTGGTGGTGGCCTTGCTCTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGagcagc                                                              <  2:742974/150‑1 (MQ=255)
                                                                                      aTGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATg                                                                                                                                               >  1:506820/1‑66 (MQ=255)
                                                                                      aTGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATg                                                                                                                                               <  2:506820/66‑1 (MQ=255)
                                                                                       tGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAgcggcg                                                          >  1:1264582/1‑150 (MQ=255)
                                                                                             ccTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAg                                                    <  1:1168996/150‑1 (MQ=255)
                                                                                               tGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGcc                                                  <  1:92958/150‑1 (MQ=255)
                                                                                                 cGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCAt                                                <  1:1056522/150‑1 (MQ=255)
                                                                                                    gATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATc                                                                 >  2:2251697/1‑130 (MQ=255)
                                                                                                    gATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATc                                                                 <  1:2251697/130‑1 (MQ=255)
                                                                                                     aTCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGAc                                            <  1:193206/150‑1 (MQ=255)
                                                                                                                   gctgACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCaa                              >  2:1206165/1‑150 (MQ=255)
                                                                                                                     tgACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGACCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAgg                            <  1:1880610/150‑1 (MQ=255)
                                                                                                                        ccGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCg                         <  1:101674/150‑1 (MQ=255)
                                                                                                                         cGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCggg                        >  1:594444/1‑150 (MQ=255)
                                                                                                                           tGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGgcg                      >  2:2397300/1‑150 (MQ=255)
                                                                                                                                  ggcggcGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTg                                                                                                              >  2:919602/1‑55 (MQ=255)
                                                                                                                                  ggcggcGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTg                                                                                                              <  1:919602/55‑1 (MQ=255)
                                                                                                                                  ggcggcGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCgc               <  2:1811161/150‑1 (MQ=255)
                                                                                                                                   gcggcgTTCATGGCGGCCATGACCACCGGCATGCTCCTGCTGGTGGTGGACTTGCTCTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCgca              <  1:2308553/150‑1 (MQ=255)
                                                                                                                                       tgTTCATGGCCGCCATGAACGCCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGcagcag          >  2:217531/2‑150 (MQ=255)
                                                                                                                                         ttCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGc                                                                                               <  2:2237530/63‑1 (MQ=255)
                                                                                                                                         ttCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGc                                                                                               >  1:2237530/1‑63 (MQ=255)
                                                                                                                                         ttCATGGCCGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGCAGCAGGt        >  1:487780/1‑150 (MQ=255)
                                                                                                                                          tCATGGCGGCAATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGCAGCAGGtt       <  2:822540/150‑1 (MQ=255)
                                                                                                                                           cATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGCAGCAGGttt      <  2:1664996/150‑1 (MQ=255)
                                                                                                                                            aTGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGCAGCAGGtttt     <  1:226733/150‑1 (MQ=255)
                                                                                                                                            aTGGCCGCCATGAACGCCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGCAGCAGGtttt     <  1:217531/150‑1 (MQ=255)
                                                                                                                                             tGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCAt                                                                                                   <  1:1223678/55‑1 (MQ=255)
                                                                                                                                             tGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCAt                                                                                                   >  2:1223678/1‑55 (MQ=255)
                                                                                                                                             tGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCATCGCAGACGGTCTGGTGCCGTATCCTGTACGGGCAGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGCAGCAGGTTTTg    <  1:1741379/150‑1 (MQ=255)
                                                                                                                                               gcggcCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGCAGCAGGTTTTGTc  >  2:819675/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                                   
TCATCCCGACTGCTTTCGAACTGGCCGGGCTGCTTGGCCCGATCATGATCGGCCTGGCCTCGGACAAACTGTTTGGCGCCCGGCGCATGCCAGCCTGCGTGATCAGCCTGGTGCTGCTGACCGTGACATTGGCGGCGTTCATGGCGGCCATGAACACCGGCAGCGTGCTGCTGGTGGTGGCCTTGCTGTTCGTCATGGGCCTGACCCTGTACGGGCCGGACTCGATGATCAGCGGCGCTGCAGCCATCGACTTCGGCACCGCCAAGGCCGGCGCCACCGCAGCAGGTTTTGTC  >  NC_002947/2893871‑2894163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑