BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_002947	3,001,858	C→G	5.4%	A180G (GCC→GGC)	PP_2625 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_002947	3,001,858	0	C	G	5.4%	354.2 / 8.9	114	A180G (GCC→GGC)	PP_2625	hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (53/53); new base G (3/3); total (56/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.72e-01

Only 100 of 115 total aligned reads displayed.

AATCAGTTGCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGCACA  >  NC_002947/3001709‑3002003

aatccgctGCTTACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGc                                                                                                                                                   >  1:215549/8‑150 (MQ=255)

     gTTGCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGGACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCcgggc                                                                                                                                              >  1:1976350/1‑150 (MQ=255)

        gCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCcgggcggg                                                                                                                                           <  1:1516606/150‑1 (MQ=255)

        gCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGCCCGATCGAGGCAAGCTGGGTCAAGGGACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGCCGCCCTGGCAAGGGATGGAGAACGGCCAAGTGCTCGATGCcgggcggg                                                                                                                                           >  2:1611645/1‑150 (MQ=255)

          aTACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGc                                                                                                                                         <  2:1976350/150‑1 (MQ=255)

          aTACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGc                                                                                                                                         <  1:250661/150‑1 (MQ=255)

             cAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACTCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGCGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCGTg                                                                                                                                      >  1:960118/1‑150 (MQ=255)

              aGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGc                                                                                                                                     >  2:1350957/1‑150 (MQ=255)

                    tctcCCGGCTACAGCTGATTGCCGGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCa                                                                                                                               <  2:629999/150‑1 (MQ=255)

                    tctcCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCa                                                                                                                               <  2:1126916/150‑1 (MQ=255)

                    tctcCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCa                                                                                                                               >  2:400407/1‑150 (MQ=255)

                      tcCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATc                                                                                                                             <  2:896084/150‑1 (MQ=255)

                      tcCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATc                                                                                                                             <  2:709607/150‑1 (MQ=255)

                      tcCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTAAGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATc                                                                                                                             >  1:1841785/1‑150 (MQ=255)

                        ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGa                                                                                                                           <  1:1415273/150‑1 (MQ=255)

                        ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGa                                                                                                                           >  1:1446993/1‑150 (MQ=255)

                        ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGa                                                                                                                           >  2:1472282/1‑150 (MQ=255)

                        ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGa                                                                                                                           >  1:78673/1‑150 (MQ=255)

                        ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGa                                                                                                                           >  1:1736453/1‑150 (MQ=255)

                        ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGa                                                                                                                           >  1:1613466/1‑150 (MQ=255)

                        ccGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGAGGGAGCCCGGCCAATTGAAGAATGCCGAGCGGGGCCTGCCCGCCATCGa                                                                                                                           >  2:633152/1‑150 (MQ=255)

                         cGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGTCGGGCAGGCCCCGCCCGGCATCGAg                                                                                                                          <  2:275743/150‑1 (MQ=255)

                           gCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCa                                                                                                                        <  2:610345/150‑1 (MQ=255)

                              aCAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATc                                                                                                                             >  1:282255/1‑142 (MQ=255)

                              aCAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATc                                                                                                                             <  2:282255/142‑1 (MQ=255)

                               cAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATc                                                                                                                    >  1:16050/1‑150 (MQ=255)

                                aGCTGATTGCCAGGCGTCCGATCGCGGCAAGCTGGTTCAAGGTACTCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCa                                                                                                                   >  1:54253/1‑150 (MQ=255)

                                  cTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGa                                                                                                                           <  1:174518/140‑1 (MQ=39)

                                  cTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGa                                                                                                                           >  2:174518/1‑140 (MQ=39)

                                  cTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAgg                                                                                                                 >  1:220907/1‑150 (MQ=255)

                                   tGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGa                                                                                                                >  1:706300/1‑150 (MQ=255)

                                   tGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGa                                                                                                                <  2:220907/150‑1 (MQ=255)

                                     aTTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAg                                                                                                              <  2:2075321/150‑1 (MQ=255)

                                                tCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGc                                                                                                                                     <  2:1115868/116‑1 (MQ=255)

                                                tCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGc                                                                                                                                     >  1:1115868/1‑116 (MQ=255)

                                                  cGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGccc                                                                                                 <  2:1446993/150‑1 (MQ=255)

                                                   gATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATc                                                                                                        >  2:580324/1‑142 (MQ=255)

                                                   gATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATc                                                                                                        <  1:580324/142‑1 (MQ=255)

                                                   gATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCa                                                                                                >  2:178078/1‑150 (MQ=255)

                                                     tCGAGGCAAGCTGGGTCAAGGTAAGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGc                                                                                              <  2:1841785/150‑1 (MQ=255)

                                                        aGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCCGCGTa                                                                                           >  1:327158/1‑150 (MQ=255)

                                                        aGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTa                                                                                           >  1:1342589/1‑150 (MQ=255)

                                                              gCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGGCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAgg                                                                                     >  2:489293/1‑150 (MQ=255)

                                                               cTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGa                                                                                                                           >  1:1526469/1‑111 (MQ=38)

                                                               cTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGGCGGGCAGGCCCCGCCCGGCATCGa                                                                                                                           <  2:1526469/111‑1 (MQ=37)

                                                                  ggTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTtgt                                                                                 <  1:1002000/150‑1 (MQ=255)

                                                                    tCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACTGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtga                                                                               <  2:178536/150‑1 (MQ=255)

                                                                    tCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtga                                                                               <  1:1168333/150‑1 (MQ=255)

                                                                    tCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtga                                                                               <  1:1350957/150‑1 (MQ=255)

                                                                    tCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtga                                                                               <  2:1891162/150‑1 (MQ=255)

                                                                     cAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGtt                                                                                   <  1:483284/145‑1 (MQ=255)

                                                                     cAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGtt                                                                                   <  1:483249/145‑1 (MQ=255)

                                                                     cAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGtt                                                                                   >  2:483249/1‑145 (MQ=255)

                                                                              ccTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACgg                                                                     >  1:267032/1‑150 (MQ=255)

                                                                                tGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATAGAGGTtgt                                                                                 <  2:2181798/136‑1 (MQ=255)

                                                                                tGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATAGAGGTtgt                                                                                 >  1:2181798/1‑136 (MQ=255)

                                                                                 gAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGa                                                                  <  2:54253/150‑1 (MQ=255)

                                                                                 gAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGa                                                                  <  1:271419/150‑1 (MQ=255)

                                                                                    cGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGt                                                               <  2:78673/150‑1 (MQ=255)

                                                                                     ggggAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATAGAGCAGATGAGTAGGCGGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTc                                                              <  2:215549/150‑1 (MQ=255)

                                                                                           ggCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATAGGGGGTGTGGGTGGTGACGGCGAGGTCGACggg                                                        >  1:953286/1‑150 (MQ=255)

                                                                                            gCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGCACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGc                                                       <  1:633152/150‑1 (MQ=255)

                                                                                            gCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGAt                                                                                                         <  1:1108130/100‑1 (MQ=255)

                                                                                            gCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGAt                                                                                                         >  2:1108130/1‑100 (MQ=255)

                                                                                             cTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCt                                                      <  1:2139475/150‑1 (MQ=255)

                                                                                                gTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGAc                                                   <  2:2202958/150‑1 (MQ=255)

                                                                                                   tACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTgtgagtga                                                                           <  1:1096111/123‑1 (MQ=255)

                                                                                                      ccGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGa                                             <  1:1352926/150‑1 (MQ=255)

                                                                                                      ccGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGa                                             >  1:1249566/1‑150 (MQ=255)

                                                                                                      ccGTGAGACGCCCTGGCAAGTGATGGAGAACGGACAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGa                                             >  2:903573/1‑150 (MQ=255)

                                                                                                         tGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATgc                                          <  1:178078/150‑1 (MQ=255)

                                                                                                           agaCGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATgcgc                                        >  2:1798115/1‑150 (MQ=255)

                                                                                                             aCGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCaa                                      >  2:1083069/1‑150 (MQ=255)

                                                                                                               gCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATc                                                                                                                             <  2:521625/61‑1 (MQ=255)

                                                                                                               gCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATc                                                                                                                             >  1:521625/1‑61 (MQ=255)

                                                                                                               gCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTCTCCGTGCTGGGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAgg                                    >  1:150075/1‑150 (MQ=255)

                                                                                                                 ccTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCcgggcggg                                                                                                                                           <  2:359043/45‑1 (MQ=255)

                                                                                                                 ccTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCcgggcggg                                                                                                                                           >  1:359043/1‑45 (MQ=255)

                                                                                                                  cTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGTCCGCCATCGAGCAGATCAGGAAGCTGAt                                                                                                         <  2:2235855/78‑1 (MQ=255)

                                                                                                                          tGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCt                         <  1:1083069/150‑1 (MQ=255)

                                                                                                                          tGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCt                         >  1:767003/1‑150 (MQ=255)

                                                                                                                            aTGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTg                       >  1:1745286/1‑150 (MQ=255)

                                                                                                                             tGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGa                      <  1:2213784/150‑1 (MQ=255)

                                                                                                                                  aaCGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGc                 >  1:1527967/1‑150 (MQ=255)

                                                                                                                                   aCGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGcc                <  2:1613466/150‑1 (MQ=255)

                                                                                                                                     ggCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCgg              <  2:327158/150‑1 (MQ=255)

                                                                                                                                     ggCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCgg              <  1:1482135/150‑1 (MQ=255)

                                                                                                                                         aagaaaccGATGTCGGGGTGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCATTGATCAGCCGGgcgc          <  2:1342589/143‑1 (MQ=255)

                                                                                                                                         aaGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGgcgc          >  1:1438313/1‑150 (MQ=255)

                                                                                                                                              cTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGc     >  2:902177/1‑150 (MQ=255)

                                                                                                                                              cTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGc     <  2:16050/150‑1 (MQ=255)

                                                                                                                                               tCGATGGCGGGCAGGCCCCGCCCGGCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGaa       <  1:827218/147‑1 (MQ=255)

                                                                                                                                               tCGATGGCGGGCAGGCCCCGCCCGGCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGaa       >  2:827218/1‑147 (MQ=255)

                                                                                                                                               tCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGca    >  1:1187864/1‑150 (MQ=255)

                                                                                                                                                 gATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGc                                                       >  1:2166441/1‑97 (MQ=255)

                                                                                                                                                 gATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGc                                                       <  2:2166441/97‑1 (MQ=255)

                                                                                                                                                 gATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCtt                                                <  1:1616445/104‑1 (MQ=255)

                                                                                                                                                 gATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCtt                                                >  2:1616445/1‑104 (MQ=255)

                                                                                                                                                 gATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGcaca  >  1:442776/1‑150 (MQ=255)

                                                                                                                                                 gATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGcaca  <  2:1187864/150‑1 (MQ=255)

AATCAGTTGCATACAGGCCATCTCCCGGCTACAGCTGATTGCCAGGCGTCCGATCGAGGCAAGCTGGGTCAAGGTACGCCTGAGCGGGGAGGGCTGGTCTACCCGTGAGACGCCCTGGCAAGTGATGGAGAACGGCCAAGTGCTCGATGCCGGGCGGGGCCTGCCCGCCATCGAGCAGATCAGGAAGCTGATCGGTGCCCAGCGTATCGAGGTTGTGAGTGATCACGGCGAGGTCGACGGGCTGACCTTCGATGCGCAAGGCCTGGCCCCCTTGATCAGCCGGGCGCGAAGCACA  >  NC_002947/3001709‑3002003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`