Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,049,417 C→T 5.1% S178S (AGC→AGT)  PP_2662 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,049,4170CT 5.1% 237.4 / 2.3 80S178S (AGC→AGT) PP_2662hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (38/37);  new base T (2/2);  total (41/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.77e-01

GGCGAGGTGCTGATCGGCAAGGACTTCGGGCTGTTCGCCCGATCCAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGACACCAACGACAGCAGCCCGACCGGAAAGGCCTACCAGGAAA  >  NC_002947/3049274‑3049553
                                                                                                                                               |                                                                                                                                         
ggCGAGGTGCTGATCGGCAAGGACTTCGGGCTGTTCGCCCGATCCAACATCCTGCTCGACGAACTGCTGGCTGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTAc                                                                                                                                     <  1:292863/150‑1 (MQ=255)
ggCGAGGTGCTGATCGGCAAGGACTTCGGGCTGTTCGCCCGATCCAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTAc                                                                                                                                     >  2:936932/1‑150 (MQ=255)
          tGATCGGCAAGGACTTCGGGCTGTTCGCCCGATCCAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACACTGACCACGGGAACCCATACTCGt                                                                                                                           >  2:2244469/1‑150 (MQ=255)
                 cAAGGACTTCGGGCTGTTCGCCCGATCCAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTAc                                                                                                                    >  2:1326541/1‑150 (MQ=255)
                   aGGACTTCGGGCTGTTCGCCCGATCCAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCt                                                                                                                  <  1:2051280/150‑1 (MQ=255)
                    ggACTTCGGGCTGTTCGCCCGATCCAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTcc                                                                                                                        >  2:643809/1‑143 (MQ=255)
                    ggACTTCGGGCTGTTCGCCCGATCCAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTcc                                                                                                                        <  1:643809/143‑1 (MQ=255)
                    ggACTTCGGGCTGTTCGCCCGATCCAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTc                                                                                                                 >  1:2013789/1‑150 (MQ=255)
                        ttCGGGCTGTTCGCCCGATCCAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCCGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAg                                                                                                             >  2:348575/1‑150 (MQ=255)
                            ggCTGTTCGCCCGATCCAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGTGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCa                                                                                                         <  2:196249/150‑1 (MQ=255)
                              cTGTTCGCCCGATCCAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCAcc                                                                                                       >  1:282134/1‑150 (MQ=255)
                                    gCCCGATCCAACATCCTGCTCGACGAACTGCTGGCCGGTTCTGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGt                                                                                                 >  1:2292680/1‑150 (MQ=255)
                                      ccGATCCAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGTTCCGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTAc                                                                                               <  1:1620886/150‑1 (MQ=255)
                                      ccGATCCAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTAc                                                                                               <  1:1878877/150‑1 (MQ=255)
                                        gATCCAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTGCCGACCTCACAGATCAAATAACGTACgg                                                                                             >  2:1600310/1‑149 (MQ=255)
                                         aTCCAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGcc                                                                                            >  1:67350/1‑150 (MQ=255)
                                           ccAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCg                                                                                          >  1:547263/1‑150 (MQ=255)
                                            cAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACACATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCgg                                                                                         >  1:1122200/1‑149 (MQ=255)
                                              aCATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGa                                                                                       <  1:1326541/150‑1 (MQ=255)
                                                  ccTGCTAGACGAACTGCTGGCCGCTTATGGCCAGGTCAGCGACACGCTGGGGGTCGTGGGCGGCGGCGGGGTGTCGTTCGGCAACGTTGGCAGGGGTTACCCATACCCGTTACCTACCTCACAGATCACGTCCCCCAAGCCCGtcctcga                                                                                   >  2:400717/1‑144 (MQ=255)
                                                   cTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGTGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑Ag                                                                                 <  2:2336445/150‑1 (MQ=255)
                                                   cTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑Ag                                                                                 >  1:970471/1‑150 (MQ=255)
                                                       tCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGcc                                                                             <  2:282134/150‑1 (MQ=255)
                                                         gacgaACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTa                                                                           >  2:1349342/1‑150 (MQ=255)
                                                         gacgaACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTAACCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTa                                                                           >  1:1479689/1‑150 (MQ=255)
                                                          acgaACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTAc                                                                          >  2:655967/1‑150 (MQ=255)
                                                            gaACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGTGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATg                                                                                     <  1:2086039/138‑1 (MQ=255)
                                                            gaACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGTGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATg                                                                                     >  2:2086039/1‑138 (MQ=255)
                                                            gaACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACgg                                                                        >  2:1622728/1‑150 (MQ=255)
                                                               ctgctgGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGtt                                                                     <  1:1645732/150‑1 (MQ=255)
                                                                tgctgGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGTGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTg                                                                    >  2:650043/1‑150 (MQ=255)
                                                                  ctgGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGc                                                                                                                                           >  1:504945/1‑78 (MQ=255)
                                                                  ctgGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGc                                                                                                                                           <  2:504945/78‑1 (MQ=255)
                                                                     gCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCAGACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTg                                                               <  2:1780854/150‑1 (MQ=255)
                                                                      ccGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTgg                                                              <  1:400717/150‑1 (MQ=255)
                                                                       cGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTa                                                                           >  2:970335/1‑136 (MQ=255)
                                                                       cGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTa                                                                           <  1:970335/136‑1 (MQ=255)
                                                                             tGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGcatcat                                                       <  2:67350/150‑1 (MQ=255)
                                                                               gCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGTGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTcac                                                                                                               <  1:1498825/93‑1 (MQ=255)
                                                                               gCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTcac                                                                                                               >  2:1498825/1‑93 (MQ=255)
                                                                                ccAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGa                                                    >  1:908716/1‑150 (MQ=255)
                                                                                ccAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGa                                                    <  2:2292680/150‑1 (MQ=255)
                                                                                ccAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGa                                                    >  1:202826/1‑150 (MQ=255)
                                                                                 cAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGAc                                                   >  2:967038/1‑150 (MQ=255)
                                                                                 cAGGTCAGCGACACCCTGGGGCTGGTGGACGACGGCGGGTTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGAc                                                   >  1:1717466/1‑150 (MQ=255)
                                                                                  aGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTa                                                                                                >  1:1702704/1‑105 (MQ=255)
                                                                                  aGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTa                                                                                                <  2:1702704/105‑1 (MQ=255)
                                                                                    gTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCa                                                                                                         >  2:611419/1‑94 (MQ=255)
                                                                                    gTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCa                                                                                                         <  1:611419/94‑1 (MQ=255)
                                                                                         cGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTa                                                                                                     <  1:1321177/93‑1 (MQ=255)
                                                                                         cGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTa                                                                                                     >  2:1321177/1‑93 (MQ=255)
                                                                                            cacCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGacac                                        <  2:610570/150‑1 (MQ=255)
                                                                                               ccTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGACACCaa                                     >  1:2036848/1‑150 (MQ=255)
                                                                                               ccTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGACACCaa                                     >  2:241415/1‑150 (MQ=255)
                                                                                                ctcgggTTGGGGGACGGTGGCGCAGTGTCGCGCGTCAACATTCGCAGCTGTTAACCATACCCGTGCCCTACCTCACAGCACACCCACCGTCCGCCCGTGATGG‑AGGGCCTACGGGTTGCTGTGGGCATCATGGACCCGGTGGACACCAAc                                    <  2:970471/147‑1 (MQ=255)
                                                                                                  ggggCCGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGACACCAACGa                                  <  1:348575/150‑1 (MQ=255)
                                                                                                   gggCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGTGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGACACCAACGAc                                 <  1:650043/150‑1 (MQ=255)
                                                                                                    ggCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGACACCAACGAca                                <  2:547263/150‑1 (MQ=255)
                                                                                                     gCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGACACCAACGAcag                               <  2:2013789/150‑1 (MQ=255)
                                                                                                       tggtggACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATc                                                                                                          >  1:1710160/1‑74 (MQ=255)
                                                                                                       tggtggACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATc                                                                                                          <  2:1710160/74‑1 (MQ=255)
                                                                                                         gtggACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTAc                                                                                               <  2:1993947/83‑1 (MQ=255)
                                                                                                         gtggACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTAc                                                                                               >  1:1993947/1‑83 (MQ=255)
                                                                                                         gtggACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGACACCAACGCcagaaca                           >  2:1466664/1‑146 (MQ=255)
                                                                                                                       ggTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGACACCAACGACAGCAGCCCGACCGGAAAGGc             <  1:241415/150‑1 (MQ=255)
                                                                                                                       ggTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGACGG‑AGGGCCTACGGGTTCCGCATAAAATGGTCTACGCGGTGGACACCAACGACAGCAGCCCGACCGGAAAGGc             <  1:1622728/150‑1 (MQ=255)
                                                                                                                         tgtCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATg                                                      <  1:2301776/107‑1 (MQ=255)
                                                                                                                         tgtCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATg                                                      >  2:2301776/1‑107 (MQ=255)
                                                                                                                         tgtCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGACACCAACGACAGCAGCCCGACCGGAAAGGCCt           >  2:1711272/1‑150 (MQ=255)
                                                                                                                             gTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTg                                             <  1:1040750/112‑1 (MQ=255)
                                                                                                                             gTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTg                                             >  2:1040750/1‑112 (MQ=255)
                                                                                                                             gTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGACACCAACGACAGCAGCCCGACCGGAAAGGCCTACCa       <  1:1711272/150‑1 (MQ=255)
                                                                                                                              ttCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCCCAGATCCACTTCCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCGCAGTGGCGTTGGACACCAACGACAGCAGCCCGACCGGAAAGGCCTACCAg      <  1:2244469/150‑1 (MQ=255)
                                                                                                                              ttCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGACACCAACGACAGCAGCCCGACCGGAAAGGCCTACCAg      <  2:550891/150‑1 (MQ=255)
                                                                                                                              ttCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGACACCAACGACAGCAGCCCGACCGGAAAGGCCTACCAg      <  1:1600310/150‑1 (MQ=255)
                                                                                                                              ttCGGCAACATTGGCAGCGGTTACCCATACCCATTCGCTACCTCACAGGTCACCTACCGTACGCCCGTGATGGAAGAAACTGGCGATAACAGT‑GGTATCATGGACCCGGTGGACACCAACGACAGCAGCCCGACAGCAAAGGCCTACCAg      >  1:550891/1‑150 (MQ=255)
                                                                                                                                cGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGACACCAACGACAGCAGCCCGACCGGAAAGGCCTACCAGGa    <  1:967038/150‑1 (MQ=255)
                                                                                                                                 ggCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑a                                                                                  >  1:1448395/1‑71 (MQ=255)
                                                                                                                                 ggCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑a                                                                                  <  2:1448395/71‑1 (MQ=255)
                                                                                                                                  gCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGa                                                                                       <  2:926933/66‑1 (MQ=255)
                                                                                                                                  gCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGa                                                                                       >  1:926933/1‑66 (MQ=255)
                                                                                                                                  gCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGACACCAACGACAGCAGCCCGACCGGAAAGGCCTACCAGGaaa  >  2:1087569/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                         
GGCGAGGTGCTGATCGGCAAGGACTTCGGGCTGTTCGCCCGATCCAACATCCTGCTCGACGAACTGCTGGCCGGTTATGGCCAGGTCAGCGACACCCTGGGGCTGGTGGACGGCGGCGGGGTGTCGTTCGGCAACATTGGCAGCGGTTACCCATACCCGTTCCCTACCTCACAGATCACCTACCGTACGCCCGTGATGG‑AGGGCCTACGGGTTGCGGTGGGCATCATGGACCCGGTGGACACCAACGACAGCAGCCCGACCGGAAAGGCCTACCAGGAAA  >  NC_002947/3049274‑3049553

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑