Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_002947 3,097,467 G→T 5.6% E9D (GAG→GAT)  PP_2706 → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0029473,097,4670GT 5.6% 288.5 / 3.6 89E9D (GAG→GAT) PP_2706hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (44/40);  new base T (3/2);  total (47/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

TGCGGACAAGCTGGAGAGCGACATGGCACGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCATCGACTGGGACGACTGGTTGGCGCTCAAGGCGC  >  NC_002947/3097324‑3097612
                                                                                                                                               |                                                                                                                                                 
tGCGGACAAGCTGGAGAGCGACATGGCACGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCTCTGTGCACGAGTTATGACAAGCAAAGTAGATAGCGGTTACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGaa                                                                                                                                             <  1:1855020/150‑1 (MQ=255)
   ggACAAGCTGGAGAGCGACATGGCACGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGcc                                                                                                                                          >  1:2178763/1‑150 (MQ=255)
    gACAAGCTGGAGAGCGACATGGCACGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCt                                                                                                                                         >  2:931876/1‑150 (MQ=255)
    gACAAGCTGGAGAGCGACATGGCACGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCt                                                                                                                                         >  1:1160794/1‑150 (MQ=255)
     aCAAGCTGGAGAGCGACATGGCACGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTg                                                                                                                                        <  2:566182/150‑1 (MQ=255)
       aaGCTGGAGAGCGACATGGCACGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGTGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGt                                                                                                                                      <  2:1838582/150‑1 (MQ=255)
            ggAGAGCGACATGGCACGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGcc                                                                                                                                 >  2:117630/1‑150 (MQ=255)
             gagaGCGACATGGCACGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGccc                                                                                                                                <  1:1078797/150‑1 (MQ=255)
               gagCGACATGGCACGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTg                                                                                                                                        <  1:2205715/140‑1 (MQ=255)
               gagCGACATGGCACGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTg                                                                                                                                        >  2:2205715/1‑140 (MQ=255)
                    aCATGGCACGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTacga                                                                                                                         <  1:1646330/150‑1 (MQ=255)
                      aTGGCACGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGTGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTg                                                                                                                                        <  1:2099514/133‑1 (MQ=255)
                      aTGGCACGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTg                                                                                                                                        >  2:2099514/1‑133 (MQ=255)
                         gCACGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGGAAAGGGGGCAGCGAGGAAGCATTCATAGGCCGCCAACTCCCCATTGCCTATCGTGTAGCGGGGTAGGCTCAGTAAGACGGCa                                                                                                                    >  2:397938/1‑150 (MQ=255)
                            cGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCa                                                                                                                 <  1:931876/150‑1 (MQ=255)
                            cGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCa                                                                                                                 <  1:219133/150‑1 (MQ=255)
                              gTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGt                                                                                                               <  1:1483052/150‑1 (MQ=255)
                                   cccATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCg                                                                                                          >  1:2118086/1‑150 (MQ=255)
                                     cATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCGGACAAGCAAAGGGGACAACGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGgc                                                                                                        >  2:700698/1‑150 (MQ=255)
                                      aggaCTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGc                                                                                                                                           <  2:194749/111‑1 (MQ=255)
                                      aTTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGc                                                                                                                                           >  1:194749/1‑114 (MQ=255)
                                         aCTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGgcgcgc                                                                                                    <  1:117630/150‑1 (MQ=255)
                                                gTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCg                                                                                             <  1:1608962/150‑1 (MQ=255)
                                                 tGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCgg                                                                                            >  1:1181374/1‑150 (MQ=255)
                                                   aCGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGAt                                                                                          >  2:268131/1‑150 (MQ=255)
                                                    cGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATg                                                                                         <  1:661688/150‑1 (MQ=255)
                                                      ccTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCCAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGcc                                                                                       <  2:4081/150‑1 (MQ=255)
                                                        tGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTa                                                                                     >  1:1867528/1‑150 (MQ=255)
                                                         gATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTg                                                                                                                                        <  1:211298/98‑1 (MQ=255)
                                                         gATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTg                                                                                                                                        >  2:211298/1‑98 (MQ=255)
                                                             gAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACa                                                                                                                    >  2:12842/1‑114 (MQ=255)
                                                             gAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACa                                                                                                                    <  1:12842/114‑1 (MQ=255)
                                                             gAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTaccacc                                                                                <  2:127629/150‑1 (MQ=255)
                                                               aCGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCAt                                                                              >  1:865228/1‑150 (MQ=255)
                                                               aCGTCGCGCTGTGCACGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCAt                                                                              <  1:1052016/150‑1 (MQ=255)
                                                                cGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGgcgcgc                                                                                                    >  2:1043829/1‑127 (MQ=255)
                                                                cGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATg                                                                             >  1:1292846/1‑150 (MQ=255)
                                                                cGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCGTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGgcgcgc                                                                                                    <  1:1043829/127‑1 (MQ=255)
                                                                        tgtgCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCtt                                                                     <  1:268131/150‑1 (MQ=255)
                                                                        tgtgCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCtt                                                                     >  2:690716/1‑150 (MQ=255)
                                                                        tgtgCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCtt                                                                     <  2:112131/150‑1 (MQ=255)
                                                                          tgCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCg                                                                   <  2:1603762/150‑1 (MQ=255)
                                                                            tccGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGATCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGca                                                                 <  1:397038/149‑1 (MQ=255)
                                                                            cccGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCCCCGGATGCCCACCACCCTGAACTGCTTCGcc                                                                 >  2:799393/1‑149 (MQ=255)
                                                                             ccGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGcac                                                                >  1:223884/1‑150 (MQ=255)
                                                                             ccGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGcac                                                                >  2:1541517/1‑150 (MQ=255)
                                                                              cGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGATCTTGAAGCCTGGTACGCCCAGTACGCCGACATCAGTTACCGCCGCTCATCACCTGATTCCTACCACCATGAACTGGTTGGcaca                                                               >  2:397038/1‑150 (MQ=255)
                                                                              cGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGcaca                                                               >  2:1972406/1‑150 (MQ=255)
                                                                              cGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGcaca                                                               >  1:245498/1‑150 (MQ=255)
                                                                              cGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGcaca                                                               >  1:1923797/1‑150 (MQ=255)
                                                                                 gTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGcc                                                            <  2:1292846/150‑1 (MQ=255)
                                                                                  ttCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCg                                                           >  2:1074526/1‑150 (MQ=255)
                                                                                     tGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAgg                                                        >  2:1547253/1‑150 (MQ=255)
                                                                                     tGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAgg                                                        >  1:715856/1‑150 (MQ=255)
                                                                                      gACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGc                                                       >  1:1607224/1‑150 (MQ=255)
                                                                                      caCAACCACAGCGGACATCGAGGCCGCTTTCATGAGCATCGAACACACCATTCCCGAGCTTTAAACCTAGTACGCCCCGTACGACACCATCAGCTACCGGCGCGCGTCACCCGATTCCTACCACCATGACCTACGTCGCGCCGCCGAGGc                                                       >  2:978288/2‑150 (MQ=255)
                                                                                       aCAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCa                                                                                                                 >  2:336508/1‑91 (MQ=255)
                                                                                       aCAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCa                                                                                                                 <  1:336508/91‑1 (MQ=255)
                                                                                           gCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGAAATCAGTTACCGGCGCGCGTCACCGGATGCCTacca                                                                                  <  2:1763505/118‑1 (MQ=255)
                                                                                           gCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGAAATCAGTTACCGGCGCGCGTCACCGGATGCCTacca                                                                                  >  1:1763505/1‑118 (MQ=255)
                                                                                            cAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTgcg                                                 >  1:710516/1‑150 (MQ=255)
                                                                                             aaaGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGATCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTgcgc                                                >  2:2032044/1‑150 (MQ=255)
                                                                                                   gACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCgacgac                                          >  1:1561166/1‑150 (MQ=255)
                                                                                                      aGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGt                                       <  1:2125682/150‑1 (MQ=255)
                                                                                                           ggACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCAt                                  >  1:1549187/1‑150 (MQ=255)
                                                                                                               gCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTacca                                                                                  >  2:86910/1‑98 (MQ=255)
                                                                                                               gCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTacca                                                                                  <  1:86910/98‑1 (MQ=255)
                                                                                                               gCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGcc                                                      <  2:2002931/126‑1 (MQ=255)
                                                                                                               gCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGcc                                                      >  1:2002931/1‑126 (MQ=255)
                                                                                                               gCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCATCGAc                              >  1:801840/1‑150 (MQ=255)
                                                                                                                cTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCATCGACt                             <  2:715856/150‑1 (MQ=255)
                                                                                                                    cATGAGCATCGAACGCACCATTGCCGATCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCATCGACTGGga                         <  1:2032044/150‑1 (MQ=255)
                                                                                                                     aTGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCATCGACTGGgac                        <  2:1923797/150‑1 (MQ=255)
                                                                                                                       gAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGAt                                                                                          <  1:2274181/82‑1 (MQ=255)
                                                                                                                       gAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGAt                                                                                          >  2:2274181/1‑82 (MQ=255)
                                                                                                                       gAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCATCGACTGGgacga                      >  2:262570/1‑150 (MQ=255)
                                                                                                                         gCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCATCGACTGGGACGACt                    <  2:801840/150‑1 (MQ=255)
                                                                                                                          cATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAg                                                         <  1:2199940/112‑1 (MQ=255)
                                                                                                                          cATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAg                                                         >  2:2199940/1‑112 (MQ=255)
                                                                                                                           aTCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCATCGACTGGGACGACTgg                  <  1:690716/150‑1 (MQ=255)
                                                                                                                           aTCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCATCGACTGGGACGACTgg                  >  1:1246074/1‑150 (MQ=255)
                                                                                                                               aaCGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCATCGACTGGGACGACTGGTTgg              <  2:2118086/150‑1 (MQ=255)
                                                                                                                                aCGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCATCGACTGGGACGACTGGTTGgc             <  2:2178763/150‑1 (MQ=255)
                                                                                                                                  gCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCATCGACTGGGACGACTGGTTGgcgc           <  1:1541517/150‑1 (MQ=255)
                                                                                                                                      cATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAAc                                                                          >  1:1445912/1‑83 (MQ=255)
                                                                                                                                      cATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAAc                                                                          <  2:1445912/83‑1 (MQ=255)
                                                                                                                                      cATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCATCGACTGGGACGACTGGTTGGCGCTCaa       >  2:2242146/1‑150 (MQ=255)
                                                                                                                                       aTTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCATCGACTGGGACGACTGGTTGGCGCTCAAg      <  2:865228/150‑1 (MQ=255)
                                                                                                                                           ccGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCATCGACTGGGACGACTGGTTGGCGCTCAAGgcgc  >  1:1588066/1‑150 (MQ=255)
                                                                                                                                               |                                                                                                                                                 
TGCGGACAAGCTGGAGAGCGACATGGCACGGTATCCCCATTACTTTCAGTGACGCCTGATTGAACGTCGCGCTGTGCCCGAGTTCTGACAAGCAAAGGGGACAGCGAGGACGCTTTCATGAGCATCGAACGCACCATTGCCGAGCTTGAAGCCTGGTACGCCCAGTACGACGACATCAGTTACCGGCGCGCGTCACCGGATGCCTACCACCATGAACTGCTTCGCACAGCCGAGGCCCTGCGCGACGACGGTGCCATCGACTGGGACGACTGGTTGGCGCTCAAGGCGC  >  NC_002947/3097324‑3097612

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑